• Journal of Microbiology and Biotechnology
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• 제31권9호
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• pp.1241-1255
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• 2021

This study was carried out to explore a non-chemical strategy for enhancing productivity by employing some antagonistic rhizobacteria. One hundred eighteen bacterial isolates were obtained from the rhizospheric zone of various crop fields of Gangwon-do, Korea, and screened for antifungal activity against Fusarium wilt (Fusarium oxysporum f. sp. lactucae) in lettuce crop under in vitro and in vivo conditions. In broth-based dual culture assay, fourteen bacterial isolates showed significant inhibition of mycelial growth of F. oxysporium f. sp. lactucae. All of the antagonistic isolates were further characterized for the antagonistic traits under in vitro conditions. The isolates were identified on the basis of biochemical characteristics and confirmed at their species level by 16S rRNA gene sequencing analysis. Arthrobacter sulfonivorans, Bacillus siamensis, Bacillus amyloliquefaciens, Pseudomonas proteolytica, four Paenibacillus peoriae strains, and Bacillus subtilis were identified from the biochemical characterization and 16S rRNA gene sequencing analysis. The isolates EN21 and EN23 showed significant decrease in disease severity on lettuce compared to infected control and other bacterial treatments under greenhouse conditions. Two bacterial isolates, EN4 and EN21, were evaluated to assess their disease reduction and growth promotion in lettuce in field conditions. The consortium of EN4 and EN21 showed significant enhancement of growth on lettuce by suppressing disease caused by F. oxysporum f. sp. lactucae respectively. This study clearly indicates that the promising isolates, EN4 (P. proteolytica) and EN21 (Bacillus siamensis), can be commercialized and used as biofertilizer and/or biopesticide for sustainable crop production.

• 한국초지조사료학회지
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• 제41권3호
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• pp.176-182
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• 2021

Ensiling is the most preferred technology to preserve the silage quality with high nutrients by the presence of lactic acid bacteria. In this study, lactic acid bacteria RJ1 and S22 were used to make the silages from different leguminous plants such as alfalfa, hairy vetch and red clover. Experimental groups were divided into control and LAB inoculated groups. LAB inoculated group; all legumes treated with a mixture of RJ1 and S22 and made an anaerobic condition for 45d. Without the addition of LAB considered the control group. The results showed that the lactic acid content was higher in all silages in response to LAB treatment and acetic acid content was slightly increased except red clover by LAB compared to control silages. A poor silage quality marker butyric acid was reduced all legume silages in response to LAB inoculation than control silages. The organic acid is closely associated with microbial population experimental silages. We noted that higher LAB and lower yeast were found in the silage in response to LAB treatment. The contents of crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), and total digestible nutrient (TDN) were not altered significantly between control and LAB treated silages. Overall data suggested that the inclusion of additional LAB potentially enhance the silage quality and preserved the nutrients for long period.

• 비교문화연구
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• 제23권
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• pp.243-264
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• 2011

The results of 2000 Population Census explains the context of a series of incidents happening in the Latino television broadcasting market recently. That is, the rapid growth of Latino population so fast as overtaking the Black population has needed media as a means of Latino's own social interests and communication. In this context, the television broadcasting market as a marketing means of capitals targeting for the Latinos has experienced more rapid changes. In other words, there has been some changes in the Latino television broadcasting market which divided by two major networks, Univision and Telemundo. It was 1970s when Latino media started to be considered as an important framework to understand the problems of the Latinos in American society. Experiencing the human rights movement of the 1960s, the Latino communities' sense of identity realized the importance of media as an expression of themselves from the interest on the factors which directly determine the quality of their life such as the immigration, education, health, and employment. The anglo media plays a role in introducing the Latinos and forming the images of the Latinos to the non-Latinos. It can be possible to criticize that the anglo media propagates the unilateral image of the Latinos by the mainstream white society, the stereotyped images of the Latinos. The spanish media targeting for the Latinos has grown continuously, combining the inside needs of forming the identity of the Latinos and communication and outside needs of commercialism. On the other hand, the needs for the programs based on the American Latinos has been increased, along with the increase of the Latino media based on the dual languages or English. This paper reviewed the history of the Latino media briefly, and then examined the relationship between the Latinos and the media through the television broadcasting which influence the Latino's everyday life enormously.

• Molecules and Cells
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• 제42권2호
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• pp.183-188
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• 2019

Osteoarthritis (OA) is a naturally occurring, irreversible disorder and a major health burden. The disease is multifactorial, involving both physiological and mechanical processes, but calcium crystals have been associated intimately with its pathogenesis. This study tested the hypothesis that these crystals have a detrimental effect on the differentiation of osteoclasts and bone homeostasis. This study employed an osteoblastosteoclast coculture system that resembles in vivo osteoblastdependent osteoclast differentiation along with $Ca^{2+}$-phosphate-coated culture dishes. The calcium-containing crystals upregulated the expression of RANKL and increased the differentiation of osteoclasts significantly as a result. On the other hand, osteoblast differentiation was unaffected. MicroRNA profiling showed that dual-specificity phosphatases 1 (DUSP1) was associated with the increased RANKL expression. DUSP1 belongs to a family of MAPK phosphatases and is known to inactivate all three groups of MAPKs, p38, JNK, and ERK. Furthermore, knockdown of DUSP1 gene expression suggested that RANKL expression increases significantly in the absence of DUSP1 regulation. Microarray analysis of the DUSP1 mRNA levels in patients with pathological bone diseases also showed that the downregulated DUSP1 expression leads to increased expression of RANKL and consequently to the destruction of the bone observed in these patients. These findings suggest that calcium-containing crystals may play a crucial role in promoting RANKL-induced osteoclastogenesis via DUSP1.

• 한국유기농업학회지
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• 제26권4호
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• pp.649-660
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• 2018

Colletotrichum acutatum에 의해 발생하는 고추탄저병을 방제하기 위해 토양 근권으로부터 분리한 미생물 중 길항효과가 있는 미생물을 선발하여 주요 작물병원균에 대한 항균활성 효과와 포장에서의 방제효과를 검정하였다. 기내 대치배양법을 이용한 길항력을 검정하였을 때, CAB13001 균주는 고추 탄저병을 일으키는 Colletotrichum acutatum을 포함한 식물 병원균 Sclerotinia cepivorum, Sclerotinia sclerotium, Botrytis cinerea에 대한 뛰어난 항균활성 효과를 보였다. 또한 풋고추를 이용한 생체 검정에서 선발균주는 고추 탄저병의 병반진전 억제효과가 우수하였으며, 포장에서의 방제가 역시 무처리 대비 82.4%로 매우 우수하였다. 이러한 결과로 볼 때 CAB13001 균주는 공기 전염을 하는 고추 탄저병의 생물적 방제제로 매우 유용할 것으로 판단된다. 한편, 본 연구에 사용된 길항미생물 CAB13001 균주는 16s rRNA gene의 계통학적 위치를 분석한 결과 Burkholderia lata로 동정하였다.

• 한국콘텐츠학회논문지
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• 제21권1호
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• pp.662-673
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• 2021

본 연구는 다문화 청소년의 문화적응 스트레스가 정신건강에 미치는 영향에서 사회적 지지의 매개 효과 규명을 목적으로 한다. 연구방법은 다문화 청소년 패널 제4차(중1)~제6차(중3)의 1,573명을 AMOS 22.0으로 분석한 결과는 다음과 같다. 첫째, 다문화 청소년의 문화적응 스트레스는 정신건강에 정적인 영향을 미치는 것으로 나타났다. 둘째, 다문화 청소년의 문화적응 스트레스는 사회적 지지에 부적인 영향을 미치는 것으로 나타났다. 셋째, 다문화 청소년의 사회적 지지는 정신건강에 부적인 영향을 미치는 것으로 나타났다. 넷째 다문화 청소년의 사회적 지지는 문화적응 스트레스가 정신건강에 미치는 영향에서 매개영향을 미치는 것으로 나타났다. 이는 다문화 청소년이 이중문화에 적응하는 과정에서 겪는 스트레스로 인하여 사회적 지지에 부적인 영향을 미치고, 이런 사회적 지지는 다문화 청소년의 정신건강에 정적인 영향을 미치는 것을 밝혔다.

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1029-1039
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• 2024

This study explores beneficial bacteria isolated from the roots and rhizosphere soil of Khao Rai Leum Pua Phetchabun rice plants. A total of 315 bacterial isolates (KK001 to KK315) were obtained. Plant growth-promoting traits (phosphate solubilization and indole-3-acetic acid (IAA) production), and antimicrobial activity against three rice pathogens (Curvularia lunata NUF001, Bipolaris oryzae 2464, and Xanthomonas oryzae pv. oryzae) were assessed. KK074 was the most prolific in IAA production, generating 362.6 ± 28.0 ㎍/ml, and KK007 excelled in tricalcium phosphate solubilization, achieving 714.2 ± 12.1 ㎍/ml. In antimicrobial assays using the dual culture method, KK024 and KK281 exhibited strong inhibitory activity against C. lunata, and KK269 was particularly effective against B. oryzae. In the evaluation of antimicrobial metabolite production, KK281 and KK288 exhibited strong antifungal activities in cell-free supernatants. Given the superior performance of KK281, taxonomically identified as Bacillus sp. KK281, it was investigated further. Lipopeptide extracts from KK281 had significant antimicrobial activity against C. lunata and a minimum inhibitory concentration (MIC) of 3.1 mg/ml against X. oryzae pv. oryzae. LC-ESI-MS/MS analysis revealed the presence of surfactin in the lipopeptide extract. The crude extract was non-cytotoxic to the L-929 cell line at tested concentrations. In conclusion, the in vitro plant growth-promoting and disease-controlling attributes of Bacillus sp. KK281 make it a strong candidate for field evaluation to boost plant growth and manage disease in upland rice.

• The Plant Pathology Journal
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• 제40권4호
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• pp.346-357
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• 2024

This study was carried out to screen the antifungal activity against Colletotrichum acutatum, Colletotrichum dematium, and Colletotrichum coccodes. Bacterial isolate GP-P8 from pepper soil was found to be effective against the tested pathogens with an average inhibition rate of 70.7% in in vitro dual culture assays. 16S rRNA gene sequencing analysis result showed that the effective bacterial isolate as Bacillus siamensis. Biochemical characterization of GP-P8 was also performed. According to the results, protease and cellulose, siderophore production, phosphate solubilization, starch hydrolysis, and indole-3-acetic acid production were shown by the GP-P8. Using specific primers, genes involved in the production of antibiotics, such as iturin, fengycin, difficidin, bacilysin, bacillibactin, surfactin, macrolactin, and bacillaene were also detected in B. siamensis GP-P8. Identification and analysis of volatile organic compounds through solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) revealed that acetoin and 2,3-butanediol were produced by isolate GP-P8. In vivo tests showed that GP-P8 significantly reduced the anthracnose disease caused by C. acutatum, and enhanced the growth of pepper plant. Reverse transcription polymerase chain reaction analysis of pepper fruits revealed that GP-P8 treated pepper plants showed increased expression of immune genes such as CaPR1, CaPR4, CaNPR1, CaMAPK4, CaJA2, and CaERF53. These results strongly suggest that GP-P8 could be a promising biocontrol agent against pepper anthracnose disease and possibly a pepper plant growth-promoting agent.

• 한국의류학회지
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• 제28권12호
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• pp.1545-1553
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• 2004

이차원적 문화적응이론은 개인이 새로운 문화를 접했을 때 그 문화를 받아들이기 위해서 모문화를 포기하는 것을 가정하는 기존의 일차원적 문화적응이론에 비하여 개인이 모문화와 새로운 문화를 함께 유지할 수 있음을 인정한다. 본 연구는 (1)이러한 이차원적 관점의 문화적응이론을 바탕으로 한국계 미국인들을 분류하고, (2) 각 하위 집단의 패션 및 관련 쇼핑행동을 살펴보는 것을 목적으로 하였다. 미국 워싱턴주에 거주하는 18-39세의 미국 영주권 또는 시민권을 소지한 미국계 한국인 소비자들을 대상으로 인터넷, 우편, 또는 한인회 행사 등을 통하여 설문조사를 실시하여(n=108). 민족정체감, 미국 체류기간, 한국문화에의 참여도, 한인 또는 타민족과의 접촉도 등과 함께 의복에 대한 관여도, 쇼핑동기, 그리고 패션준거집단의 영향 등을 측정하였다. 한국인으로서의 정체감과 미국인으로서의 정체감에 대한 점수의 중간값을 이용하여 표본집단을 이차원적 문화적응이론에서 정의하는 격리(Segregation) 집단, 동화(Assimilation) 집단, 화합(Integration) 집단으로 구분하였고, 세 집단의 패션 관련 쇼핑 행동을 다중분산분석과 Duncan 다중비교를 통해 살펴보았다. 두 문화적 특성을 함께 지닌 화합집단은 인구통계적인 면이나 민족관련 행동에 있어서는 한국적인 특성을 유지하고 있었으나 패션 및 쇼핑행동면에서는 미국인에 가까운 특성을 보여 동화집단이나 격리집단과는 구별되었다. 이 결과는 이차원적 문화적응이론을 이용하여 패션 소비자들을 이해하는 것이 유용함을 보여준다고 할 수 있다.

• The Plant Pathology Journal
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• 제33권5호
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• pp.499-507
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• 2017

In an attempt to develop a biological control agent against mycotoxigenic Fusarium species, we isolated Bacillus amyloliquefaciens strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic F. asiaticum, F. graminearum, F. proliferatum, and F. verticillioides both in vitro and in planta (maize). Further screening using dual culture extended the activity range of strain DA12 against other fungal pathogens including Botrytis cinerea, Colletotrichum coccodes, Endothia parasitica, Fusarium oxysporum, Raffaelea quercus-mongolicae, and Rhizoctonia solani. The butanol extract of the culture filtrate of B. amyloliquefaciens DA12 highly inhibited the germination of F. graminearum macroconidia with inhibition rate 83% at a concentration of $31.3{\mu}g/ml$ and 100% at a concentration of $250{\mu}g/ml$. The antifungal metabolite from the butanol extract was identified as iturin A by thin layer chromatography-bioautography. In addition, volatile organic compounds produced by DA12 were able to inhibit mycelial growth of various phytopathogenic fungi. The volatile compounds were identified as 2-heptanone, 5-methyl heptanone and 6-methyl heptanone by gas chromatography-mass spectrometry (GC-MS) analysis. These results indicate that the antagonistic activity of Bacillus amyloliquefaciens DA12 was attributable to iturin A and volatile heptanones, and the strain could be used as a biocontrol agent to reduce the development of Fusarium diseases and mycotoxin contamination of crops.