Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Dual Culture Inoculation Enhanced Quality of Silage Produced from Leguminous Plants

  • Muthusamy, Karnan (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ilavenil, Soundharrajan (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Jeong Sung (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Bae Hun (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Hyung Soo (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Ki Choon (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2021.09.02
  • Accepted : 2021.09.16
  • Published : 2021.09.30
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Abstract

Ensiling is the most preferred technology to preserve the silage quality with high nutrients by the presence of lactic acid bacteria. In this study, lactic acid bacteria RJ1 and S22 were used to make the silages from different leguminous plants such as alfalfa, hairy vetch and red clover. Experimental groups were divided into control and LAB inoculated groups. LAB inoculated group; all legumes treated with a mixture of RJ1 and S22 and made an anaerobic condition for 45d. Without the addition of LAB considered the control group. The results showed that the lactic acid content was higher in all silages in response to LAB treatment and acetic acid content was slightly increased except red clover by LAB compared to control silages. A poor silage quality marker butyric acid was reduced all legume silages in response to LAB inoculation than control silages. The organic acid is closely associated with microbial population experimental silages. We noted that higher LAB and lower yeast were found in the silage in response to LAB treatment. The contents of crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), and total digestible nutrient (TDN) were not altered significantly between control and LAB treated silages. Overall data suggested that the inclusion of additional LAB potentially enhance the silage quality and preserved the nutrients for long period.

Keywords

Acknowledgement

Cooperative Research Program for Agriculture Science and Technology Development supported funds for this research work (Project No. PJ01358902). The project titled "Technique development for the manufacture of high-quality legume silage" sponsored by RDA, Korea. This study was also supported by 2021 Postdoctoral Fellowship Program of the National Institute of Animal Science funded by RDA, Korea.

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