• Title/Summary/Keyword: dry test

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Effects of Dietary Quartz Porphyry and Feed Stimulants, BAISM Supplementation on Growth Performance and Disease Resistance of juvenile eel Anguilla japonica (사료내 맥반석과 BAISM 복합첨가가 치어기 뱀장어 Anguilla japonica의 성장과 내병성에 미치는 영향)

  • Bae, Jun-Young;Han, Kyung-Min;Lee, Jun-Ho;Kim, Sang-Eun;Lee, Jeong-Yeol;Bai, Sung-Chul C.
    • Journal of Aquaculture
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    • v.21 no.1
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    • pp.26-33
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    • 2008
  • This study investigated the synergistic effects of dietary supplementation of quartz porphyry(QP) and a laboratory developed feed stimulants, BAISM(BS) on growth performance and utilization as the additives for juvenile eel Anguilla japonica. Six isoenergetic experimental diets(18.2 kJ/g) were formulated to contain 50% crude protein, 15% lipid with or without dietary QP(Song-Gang stone, Davistone, Korea) and BS supplementation. QP and BS were provided at 0% in the control diet($Q_0B_0$) and at 0.7% QP+0% BS($Q_{0.7}B_0$), 0.7% QP+0.3% BS($Q_{0.7}B_{0.3}$), 0.7% QP+0.5% BS($Q_{0.7}B_{0.5}$), 0.7% QP+0.75% BS($Q_{0.7}B_{0.75}$) and 0.7% QP+1.0% BS($Q_{0.7}B_{1.0}$) in experimental diets on dry matter basis. After four weeks of adaptation, triplicate groups of 30 fish initially averaging $15{\pm}0.1g(mean{\pm}SD)$ were randomly distributed into each aquarium, and they were fed one of the experimental diets for 8 weeks. By the end of the feeding trial, weight gain(%), specific growth rate(%), feed efficiency(%) and protein efficiency ratio of fish fed diet $Q_{0.7}B_{0.5},\;Q_{0.7}B_{0.75}\;and\;Q_{0.7}B_{1.0}$, were significantly higher(P<0.05) than those of fish fed the other diets. But, $Q_{0.7}B_{0.5},\;Q_{0.7}B_{0.75}\;and\;Q_{0.7}B_{1.0}$ were no significant differences(P<0.05). In challenge test, fish were infected by intraperitoneal injection of 0.1 mL bacterial suspension with Edwardsiella tarda per fish after the feeding trial. As a result, fish fed QP and BS supplemented diets have a significantly higher cumulative survival rate than those of fish fed control diet(P<0.05). In conclusion, these results indicated that the optimum dietary supplementation level of QP and BS could be approximately 0.7% quartz porphyry+0.5% BAISM($Q_{0.7}B_{0.5}$) of diet based on WG, FER, SGR, PER, cumulative survival rate in juvenile eel A. japonica.

'Joseong', a New Early-Heading Forage Triticale Cultivar for Paddy Field of Double Cropping (이모작 재배에 적합한 조숙성 조사료용 트리티케일 신품종 '조성')

  • Han, Ouk-Kyu;Park, Tae-Il;Park, Hyung-Ho;Song, Tae-Hwa;Ju, Jung-Il;Jeung, Jae-Hyun;Kang, Sung-Joo;Kim, Dae-Ho;Choi, Hong-Jib;Park, Nam-Geon;Kim, Kee-Jong;Hwang, Jong-Jin;Baek, Seong-Beum;Kwon, Young-Up
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.3
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    • pp.193-202
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    • 2012
  • 'Joseong', a winter forage triticale cultivar (X Triticosecale Wittmack), was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2010. The cultivar 'Joseong' was selected from the cross FAHAD_5/RHINO1R.1D 5+10 5D'5B'//FAHAD_5 by CIMMYT (Mexico) in 1992. Subsequent generations were handled in pedigree selection program at Mexico from 1993 to 1998, and a line 'CTSS92Y-A-4Y-0M-5Y-0B' was selected for earliness and good agronomic characteristics. After preliminary and advance yield test in Korea for 2 years, the line designated as a line name of 'Iksan26'. The 'Iksan26' was subsequently evaluated for earliness and forage yield in 7 different locations such as Jeju, Iksan, Cheongwon, Yesan, Naju, Daegu, and Jinju from 2008 to 2010 and finally named as 'Joseong'. The cultivar 'Joseong' has characteristics of dark green leaf, yellow culm and spike, and large grain of yellowish brown color. The heading date of cultivar 'Joseong' was April 24, which was 5 days earlier than that of check cultivar 'Shinyoung'. It showed better tolerance or resistance to lodging, wet injury, powdery mildew, and leaf rust than those of the check cultivar 'Shinyoung'. The average forage dry matter yield of cultivar 'Joseong' at milk-ripe stage was 14.5 MT $ha^{-1}$, which was lower than 16.5 MT $ha^{-1}$ of the check cultivar 'Shinyoung'. The silage quality of 'Joseong' (5.3%) was lower to the check cultivar 'Shinyoung' (6.0%) in crude protein content, while was 2.1% superior to the check cultivar 'Shinyoung' (61.9%) in TDN (total digestible nutrients). It showed 5.1 MT $ha^{-1}$ of grain yield, which was 40% higher than that of the check cultivar 'Shinyoung' (3.65 MT $ha^{-1}$). This cultivar is recommended for fall sowing crop in the area where daily minimum mean temperatures are averaged higher than $-10^{\circ}C$ in January, and as a winter crop using whole crop forage before planting rice in around Korea.

Response of Growth and Functional Components in Baby Vegetable as Affected by LEDs Source and Luminous Intensity (LEDs 광조성 및 광도가 베이비채소의 생육 및 기능성물질에 미치는 영향)

  • Yoon, Seong-Tak;Jeong, In-Ho;Kim, Young-Jung;Han, Tae-Kyu;Yu, Je-Bin;Jae, Eun-Kyung
    • Korean Journal of Organic Agriculture
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    • v.23 no.3
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    • pp.549-565
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    • 2015
  • This study was conducted to investigate the growth characteristics and functional materials of baby vegetables as affected by different LEDs and luminous intensity at Anseongsi, Gyeonggi Province, in 2014. Test crops were beet, chicory, spinach, red leaf lettuce, crown daisy and red mustard purchased from the seed company of Dongbu Hannong and Jinheung. Growth characteristics were measured and the content of functional materials was analyzed 40 days after seeding at plug plate. Treatment of Red+Blue (4:1) at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity showed the highest number of leaves in five baby vegetables of beet, chicory, red leaf lettuce, crown daisy and red mustard. The highest shoot length of chicory, spinach, red leaf lettuce, crown daisy and red mustard was obtained from the treatment of Red+Blue (4:1) at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity. Fresh weight and dry weight of all six baby vegetables were the highest in treatment of Red+Blue (4:1) at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity. Content of chlorophyll a and chlorophyll b of spinach, red leaf lettuce and red mustard showed the highest in Fluorescent lamp at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity whereas other crops did not show definite trend under different LEDs lights and luminous intensity. The highest total content of anthocyanins and polyphenol were obtained from the treatment of Red+Blue (4:1) at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity in all six baby vegetables. Free radical scavenging activity was highest in all six vegetable crops at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity, but it was not different significantly between LEDs. As a result, the growth and the content of functional material of baby vegetables are generally to be increased in Red+Blue (4:1) at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity. Mixed light of Red+Blue is thought to give good effect on the growth and the content of functional material in baby vegetable crops. Because there are many differences in regard of LED lights, crop varieties, cultivation and experimental methods in their impact on the growth and functional materials of baby vegetables among researchers, it is considered that a more precise studies are needed for the crop responses to LED light and luminous intensity.

Sulforaphane and Total Phenolics Contents and Antioxidant Activity of Radish according to Genotype and Cultivation Location with Different Altitudes (재배지 고도에 따른 무 품종별 설포라판, 총페놀함량 및 항산화 특성)

  • Im, Ju-Sung;Lee, Eung-Ho;Lee, Jong-Nam;Kim, Ki-Deog;Kim, Hwa-Yeong;Kim, Myung-Jun
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.335-342
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    • 2010
  • Sulforaphane (SFN) and total phenolics (TPC) contents and antioxidant activity (AA) were analyzed from 13 radish genotypes (Rhaphanus sativus L.), cultivated at 3 locations with different altitudes (Gangneung: asl 5 m, Jinbu: asl 550 m, and Daegwallyeong: asl 750 m). SFN varied greatly from 0.1 to $120.5{\mu}g{\cdot}g^{-1}$ in dry weight test and was significantly affected by location ($P{\leq}0.001$), genotype ($P{\leq}0.001$) and $location{\times}genotype$ interaction ($P{\leq}0.01$). Radishes, cultivated at Daegwallyeong site, showed higher SFN than those of other locations. Among different genotypes, the root of 'Black radish' and leaves of 'Purunmu' of Daegwallyeong had the highest SFN (107.8 and $120.5{\mu}g{\cdot}g^{-1}$, respectively). TPC in root was affected by genotype ($P{\leq}0.001$), and $location{\times}genotype$ interaction ($P{\leq}0.01$), but not by location. In leaves, TPC was affected by location ($P{\leq}0.01$), genotype ($P{\leq}0.001$), and $location{\times}genotype$ interaction ($P{\leq}0.001$). AA expressed as electron donating ability was significantly influenced by location, genotype and $location{\times}genotype$ interaction and correlated positively with TPC ($Pearson's$ $r$=0.897) in root. These results suggest that radish could be a good source of functional food and high altitude location such as Daegwallyeong has potential for the production of radish with high content of health promoting factors.

Effects of TDN/CP Ratio on Nutrient Intake, Body Weight Gain, Blood Characteristics of Dairy Heifers from 12 to 14 Months of Age (TDN/CP 비율이 젖소 12~14개월령 육성우 영양소 섭취량, 증체량 및 혈액성상에 미치는 영향)

  • Kim, Gyeom-Heon;Hwang, Won-Uk;Kim, Hyun-Jin;Kim, Soo-Ki
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.2
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    • pp.115-123
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    • 2016
  • In this study, the effect of energy and protein ratio on the nutrient availability and body weight gain was determined to find the proper nutrient level for the stage of replacement dairy heifers before fertilization at approximately 12~24 months old after birth. Dry matter intake showed that the TDN/CP rate of T1 (5.0:1), T2 (4.5:1) and T3 (4.0:1) resulted in similar values. Among feces, the ash content of T1 was 11.07%, which was significantly (p < 0.05) higher than that of T3 (9.69%). It was also significantly (p < 0.05) higher than that of T2 (10.32%). When the TDN/CP rate was increased, the ash content in the feces was also increased. Regardless of the initial weight, weight gain was increased significantly as the CP rate in the feed was increased on the $30^{th}$ day. On the $60^{th}$ day, T2 showed the highest rate of gain (p < 0.05). As the TDN rate was decreased or the protein rate was increased, weight gain was significantly (p < 0.05) increased. Regarding GPT level in the blood after finishing the test, T1 and T3 (112.6, 88.3 u/l) showed significantly (p < 0.05) higher value than T2 (50.9 u/l). For phosphorus level, T1 (46.3 mg/dl) showed significantly (p < 0.05) higher value than other treatments. For HDL cholesterol level, T2 (145.2 mg/dl) had significantly (p < 0.05) higher level than T1 (121.0 mg/dl) or T3 (132.3 mg/dl). For triglyceride levels, T3 (40.6 mg/dl) had significantly (p < 0.05) higher value than T1 (20.7 mg/dl) and T2 (29.0 mg/dl). For other blood parameters including BUN concentration, there were no significant (p > 0.05) difference among treatments. As a result, although feed intake and weight gain with TDN/CP rate of 4:1 showed best results, considering the excess body fat accumulation possibilities or blood metabolism, it seems 4.5:1 ratio is most appropriate.

Application of Chlorophyll Fluorescence Parameters for the Detection of Water Stress Ranges in Grafted Watermelon Seedlings (수박접목묘의 건조스트레스 범위 탐지를 위한 엽록소형광 지수의 적용)

  • Shin, Yu Kyeong;Kim, Yong Hyeon;Lee, Jun Gu
    • Journal of Bio-Environment Control
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    • v.28 no.4
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    • pp.461-470
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    • 2019
  • This study was carried out to quantify the drought stress in grafted watermelon seedlings non-destructively by using chlorophyll fluorescence (CF) imaging technique rather than the visual judgment. Six-day old watermelon seedlings were grown under uniform irrigation for 3 days, and then given drought stress. Afterward, the sensor for the measurement of water content in plug tray cell unit was used to classify the drought-stress level into nine groups from D1 (53.0%, sufficient moisture state) to D9 (15.7%, extremely dry stress), and the 16 CF parameters were measured. In addition, re-irrigation was performed on the drought stressed seedlings(D5 - D9) to determine the growth and photosynthesis recovery level, which was not confirmed by visual judgment. The kinetic curve patterns of CF in three different drought stressed seedling groups were found to be different for the early detection of drought stress. All the 16 CF parameters decreased continuously with exposure to drought stress and drastically decreased from D5 (32.1%) where the visual judgment was possible. The fluorescence decline ratio (Rfd_Lss) started to decrease from the initial drought stress level (D5 - D6), and the Maximum PSII quantum yield (Fv/Fm) was significantly decreased in the later extreme drought stress range (D7 - D9) by re-irrigation recovery test. Thus, Rfd_Lss and Fv/Fm parameters were finally selected as potent indicators of growth and photosynthesis recovery in the initial and later stages of drought stress. Also, to the differences in the numerical values of the individual chlorophyll fluorescence parameters, the drought stress level was intuitively confirmed through the image. These results indicate that Rfd and Fv/Fm can be considered as potential CF parameters for the detection of low and extremely high drought stress, respectively. Furthermore, Fv/Fm can be considered as the best CF parameters for recovery at re-irrigation.

Assessment of Fertilizer Efficiency of Pharmaceutical Byproduct and Cosmetic Industry Wastewater Sludge as Raw Materials of Compost (제약업종 부산물 및 화장품 제조업 폐수처리오니의 비효검정)

  • Lim, Dong-Kyu;Kwon, Soon-Ik;Lee, Seung-Hwan;So, Kyu-Ho;Sung, Ki-Suk;Koh, Mun-Hwan
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.2
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    • pp.108-117
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    • 2005
  • Pharmaceutical byproduct sludge and cosmetic industry wastewater sludge can be used as a raw material of compost. Effects of three types of pharmaceutical byproduct sludge and one type of cosmetic industry wastewater sludge on soil properties and red pepper growth were investigated in a field based concrete pot ($2{\times}2m$). These sludges and pig manure ($5Mg\;ha^{-1}$, dry basis) were incorporated into the upper of loam soil 30 days prior to transplanting red pepper. Changes in soil properties and contents of heavy metals and toxic organic compounds in soil and plant were measured. And also plant growth measurement and bioassay of soil phytotoxicity were included. Contents of heavy metals were increased in the soils treated with the sludges. Plant growth in the sludge treatments were mostly inferior to that of NPK treatment, especially in early stage. Content of N in plant was lower in all sludge treatments at early and middle growth stages, and it was especially caused by characteristics and concentration of nitrogen and organic matter of sludges. Total yield of red pepper was highest in the NPK treatment and followed by pharmaceutical sludge 3, pig manure, pharmaceutical sludge 1, and pharmaceutical sludge 2, and the yield of cosmetic sludge treatment was considerably lower than others. HEM and PAHs contents in soil of cosmetic sludge treatment were $4.80mg\;kg^{-1}$ and $2,263.2{\mu}g\;kg^{-1}$, respectively. Root elongation of lettuce exposed to the water extract of soil treated with cosmetic sludge was about 20% of that found in the test with soil extract of non fertilization treatment. At present, raw materials of compost were authorized according to the contents of organic matter, heavy metals and product processing. Toxic organic compounds analysis and bioassay would be helpful for authorization and assessment of suitability of raw materials of compost.

High Quality and High Yielding Rice Variety 'Cheongdam' Adaptable to Direct Seeding (고품질 다수성 직파재배적성 신품종 '청담벼')

  • Choi, Im-Soo;Kang, Kyung-Ho;Jeong, O-Young;Jeong, Eung-Gi;Cho, Young-Chan;Kim, Yeon-Gyu;Kim, Myeong-Ki;O, Myeong-Gyu;Choi, In-Bea;Jeon, Yong-Hee;Won, Young-Jae;Shin, Young-Seoup;Oh, In-seok
    • Korean Journal of Breeding Science
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    • v.43 no.6
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    • pp.581-586
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    • 2011
  • 'Cheongdam' is a japonica rice variety developed from a cross between SR19200-HB826-34, a line of good germination ability and shoot emergence at low temperature and Juanbyeo, good quality and direct-seeding adaptable cultivar by the rice breeding team of National Institute of Crop Science, RDA in 2006. This variety has 153 days of total growth duration from seeding to maturity in direct-seeding, and 160 days of growth duration from seeding to maturity in transplanting. This is erect plant type with culm length of 74 cm, thick culm, and green leaves. It has large panicle shape with 126 and 140 spikelets per panicle in direct-seeding and transplanting, respectively. Milled rice is transluscent and medium in grain size of non-glutinous endosperm. This variety is susceptible to leaf and neck blast, bacterial blight, stripe virus disease and brown planthopper. The yield potential of 'Cheongdam' is 5.84 MT/ha at ordinary transplanting culture and 5.62 MT/ha and 5.89 MT/ha at wet direct-seeding and dry direct-seeding cultures, respectively in the local adaptability test for three years. 'Cheongdam' would be adaptable to middle and southern plain of Korea for direct-seeding culture and transplanting rice culture.

Evaluation of the Fiber Separation Method and Differences in the Storage Root Fiber Content among Sweetpotato (Ipomoea batatas L.) Varieties (고구마 괴근의 섬유질 분리 조건 탐색 및 품종별 섬유질 함량 차이)

  • Won Park;Im been Lee;Mi Nam Chung;Hyeong-Un Lee;Tae Hwa Kim;Kyo Hwui Lee;Sang Sik Nam
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.1
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    • pp.20-26
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    • 2023
  • Fiber content in the storage roots of sweetpotato varies between different varieties. For examples, the high fiber content of certain types has a poor texture when steamed or roasted. This study was conducted to evaluate the optimal sieve mesh size for separating fibers, the chemical composition of fibers and differences in fiber content among different varieties. We found that the separated fiber content (dry weight) of mashed and steamed sweetpotato was higher after washing three times (143.3 mg/100 g) compared with that washed five times (128.4 mg/100 g). The Hogammi variety remained 85.9% of total fiber content at 10 mesh (2,000 ㎛) and 9.6% of total fiber content at 30 mesh (600 ㎛), and Jinyulmi remained 74.9 and 16.7% of total fiber content , respectively. Therefore, a 30 mesh sieve was considered the most suitable for fiber separation. Among the 10 studied cultivars, Jinhongmi showed the lowest amount of fiber (24.8 mg/100 g) and Hogammi had the highest amount (111.4 mg/100 g), which was 4.5 times larger than that of Jinhongmi. Cellulose, hemicellulose and lignin content of separated fibers showed no difference between the viscous-type Hogammi and powdery-type Jinyulmi varieties, with averages of 32.5, 22.3 and 29.6%, respectively. Correlation results using the Image J program showed a significant correlation between the distribution of the stained area and the fiber content (R = 0.74, p < 0.05). Staining distribution differed among varieties, suggesting that a simple fiber content test could be performed using the staining method on raw sweetpotato. These results provide useful information to help inform farmers on the fiber content of different sweetpotato varieties.

The Effect of Single and Mixed Microbial Inoculation on the in situ Fiber Digestibility and Silage of Rice Straw Contaminated Mycotoxins (단일 및 복합 미생물 접종이 곰팡이독소 오염 볏짚의 사일리지 및 In situ 섬유소 소화율에 미치는 영향)

  • Ha Guyn Sung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.4
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    • pp.229-236
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    • 2022
  • This study was conducted to evaluate the efficacy of adding the microbial inoculants to silage for reducing mycotoxins in rice straw silage. When a single agent of L. plantarum and a mixed agent of L. plantarum and S. cerevisiae were added in rice straw silage contaminated mycotoxins, it had an effect on silage fermentation and fiber degradation as well as mycotoxin reduction. Among the mycotoxins, only ochratoxin A and zearalenone were found in the test sample. Ochratoxin A and zearalenone showed a decreasing trend with the addition of silage inoculants compared to the control groups (38.11±2.22 and 633.67±50.30 ㎍/kg), and there was a significant difference at the mixed agents; 27.78±2.28 and 392.72±25.04 ㎍/kg, respectively (p<0.05). The pH was lower in the single agent and the mixed agent compared to the control (p<0.05). The concentration of lactic acid was higher in the single agent (11.73±0.31 mM) than in the control group (8.18±0.93 mM), and the highest concentration was 16.01±0.88 mM in the mixed agent (p<0.05). Acetic acid and propionic acid were found to be significantly lowered with the addition of silage inoculants (p<0.05). Total VFA was also lower at the addition of silage inoculants than the control group (p<0.05). The rumen in situ dry matter degradation of NDF and ADF was maintained at the highest levels of the mixed agent during the culture period, followed by the single agent and the control group at the lowest level. NDF and ADF degradation showed a significant difference at all time points after 12 and 24 hours of culture, respectively (p<0.05). The study results showed that the silage inoculants had the positive effects on quality increasing of rice straw silage; fermentative charateristics, fiber degradation and mycotoxins reduction. Ochratoxin A and zearalenone were greater reduction by adding bacterial inoculants of silage. Therefore it is considered that L. plantarum and S. cerevisiae will improve the quality and stability with remediation of mycotoxin in silage.