• Asian-Australasian Journal of Animal Sciences
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• v.31 no.1
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• pp.40-46
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• 2018

Objective: To examine the effects of Rhodobacter sphaeroides (R. sphaeroides) supplementation as a direct-fed microbial (DFM) on rumen fermentation in dairy cows and on coenzyme Q10 (CoQ10) transition into milk, an in vitro rumen simulation batch culture and an in vivo dairy cow experiment were conducted. Methods: The characteristics of in vitro ruminal fermentation were investigated using rumen fluids from six cannulated Holstein dairy cows at 2 h post-afternoon feeding. A control treatment was included in the experiments based on a typified total mixed ration (TMR) for lactating dairy cows, which was identical to the one used in the in vivo study, plus R. sphaeroides at 0.1%, 0.3%, and 0.5% TMR dry matter. The in vivo study employed six ruminally cannulated lactating Holstein cows randomly allotted to either the control TMR (C-TMR) treatment or to a diet supplemented with a 0.5% R. sphaeroides culture (S-TMR, dry matter basis) ad libitum. The presence of R. sphaeroides was verified using denaturing gradient gel electrophoresis (DGGE) applied to the bacterial samples obtained from the in vivo study. The concentration of CoQ10 in milk and in the supernatant from the in vitro study was determined using high performance liquid chromatography. Results: The results of the in vitro batch culture and DGGE showed that the concentration of CoQ10 significantly increased after 2 h of R. sphaeroides supplementation above 0.1%. When supplemented to the diet of lactating cows at the level of 0.5%, R. sphaeroides did not present any adverse effect on dry matter intake and milk yield. However, the concentration of CoQ10 in milk dramatically increased, with treated cows producing 70.9% more CoQ10 than control cows. Conclusion: The CoQ10 concentration in milk increased via the use of a novel DFM, and R. sphaeroides might be used for producing value-added milk and dairy products in the future.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.4
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• pp.511-515
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• 2000

Early weaning is a means of breaking the disease cycle from sow to piglet as well as capitalising on the enormous growth potential of the pig. However, the transition from milk to dry diets results in a growth check. Feeding of supplemental milk, fermented to reduce pH and enterotoxigenic bactetial proliferation, may be a means of gradually weaning pigs on to solid feed. This study involved 216 pigs weaned from the sow at 12 days of age, allocated to groups of 6 males and 6 females per weaner pen and allowed ad libitum access to a pelleted diet. In addition, half the pigs were given supplemental fermented skim milk for the first 8 days after weaning. Feeding supplemental fermented milk increased feed intake (104 vs. 157 g DM/d, p=0.011), average daily gain (-3 vs. 112 g/d, p<0.001) and feed conversion efficiency (0.01 vs. 0.81, p=0.003) over the first 8 days after weaning. The improvements observed in the supplemented pigs continued to be augmented such that, by 42 days of age, the pigs that had received supplemental fermented milk were heavier (9.6 vs. 11.5 kg, p=0.003) than their unsupplemented counterparts. Feeding fermented supplemental milk to early-weaned pigs can improve growth performance in the immediate and subsequent post-weaning period.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.3
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• pp.253-259
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• 1997

Holstein Friesian and Jersey crossbreds are being widely introduced in the state of Gujarat in India. This paper evaluates feeding practices at farm level and examines whether the crossbreds fit into the existing mixed farm systems. Over a period of four years milk-offtake and feeds offered were recorded for 1331 cows at fortnightly intervals. The breed and the amount of concentrates fed contributed most to the variation in milk offtake. The introduction of crossbred cattle has a major impact on smallholder mixed farming systems. Crossbreds produced, on average, 1.8 times more milk than Desi, Gir, and Kankrej cows. They were fed 1.4 times more concentrates, and about 1.2 times more green and dry feeds than local cows. The major limiting constraint is the quality of the roughages offered. Farmers with crossbreds try to adjust their feeding of concentrates according to the needs of their cows. On tribal farms, local cows produced less milk than on non-tribal farms, whereas crossbreds produced the same amount of milk on both tribal and non-tribal farms. Crossbreds fit into the farming systems of both tribal and non-tribal farmers. The differences in agro-climatic characteristics between different areas in Gujarat were not reflected in differences in milk offtakes.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.10
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• pp.1409-1418
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• 2001

Two trials were carried out during two different grazing seasons to evaluate effect of grass silage supplementation, when amount of pasture is limited on dry matter intake (DMI), milk production, and gross energetic efficiency (GEE) of grazed lactating cows on a high forage-based diet. Fifty-one Holstein cows were randomly assigned to one of two dietary treatments: high pasture group or high silage group. In the spring flush, pasture and silage DMI, milk yield, milk fat percentage, and GEE were not different between the dietary groups. After the spring flush, pasture and silage DMI were higher for the high silage group than for the high pasture group. After the spring flush, although these were the higher total DMI of the high silage group than the high pasture group, milk yield was significantly (p<0.05) higher for the high pasture group than the high silage group. Milk fat percentage tended to be higher for the high silage group than the high pasture group. The GEE was significantly (p<0.05) higher for the high pasture group than the high silage group during after the spring flush. This study indicated that supplementation of grass silage, especially after the spring flush, can have a significant effect of increasing of forage intake and maintenance of the milk fat percentage; but not increase milk yield and GEE.

• Korean Journal of Agricultural Science
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• v.48 no.1
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• pp.45-57
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• 2021

This study aimed to determine the effects if ambient temperature (Ta), relative humidity (RH), and temperature-humidity index (THI) on milk production parameters such as milk yield, fat, protein, milk urea nitrogen (MUN), and lactose. A total of fifty (50) mid-lactating Korean Holstein cows (144 - 150 days postpartum) were randomly assigned to two groups in equal numbers (n = 25) according to the type of diet received: conventional feed, a mixture of 56: 44 ratio of roughage and concentrate mix, and organic feed containing 100% organic roughage. Temperature, RH, and THI were recorded daily and averaged monthly. Conventional group showed higher dry matter intake throughout the experiment and thus, showed higher total milk yield and milk composition such as milk fat, protein, and lactose content than organic milk. Milk of the conventional group showed higher polyunsaturated fatty acid concentration. Highest milk yield was observed in September (21.7℃, 82.4%RH) in conventional group (27.69 kg·day^-1) and in June (21.4℃, 72.9%RH) in organic group (14.31 kg·day^-1). In conventional group, milk yield was positively correlated with Ta and THI, and milk protein was negatively correlated with Ta, RH, and THI. In organic feeding, only the MUN among milk composition parameters showed a significant correlation where it showed a negative correlation with Ta, RH, and THI. Conventional feeding showed significantly higher milk yield, fat, protein, and lactose than organic feeding throughout the experiment, although, organic feeding showed to be less likely affected by Ta, RH, and THI than the conventional feeding.

• Journal of The Korean Society of Grassland and Forage Science
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• v.7 no.3
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• pp.153-156
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• 1987

With a purpose of evaluation rye crop as winter green feed, a cultivar was sown in autumn and harvested at booting, heading, milk, dough and yellow-ripe stages, and processed into silage. The quality of the silage was assessed in terms of ammonia-N, recovery rate in dry matter, pH, and the composition of organic acids. The results obtained are: 1. The recovery rate in dry matter from the silage was the highest when harvested at yellow stage (P<0.01). The ratio of ammonia-N to total-N was low when the rye crop was harvested during milk (9.4%) and dough ( 12.7%) stages (P$NH_3-N$. 3. Silage quality based on the composition of organic acids was highly evaluated when the crop was harvested during milk, and dough stages. 4. Considering both the DM yield and quality of the silage, it was found that rye crop should be harvested at dough stage and ensiled.

• Korean Journal of Food Science and Technology
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• v.29 no.1
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• pp.115-119
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• 1997

The chemical and microbial properties and acceptability of milk and soymilk inoculated with Bifidobacterium were studied at each storage time (0, 5, 10, 15, 20, 25, and 30 days). Soymilk, milk, low-heat milk, low-fat milk, non-fat dry milk with Bifidobacterium longum ATCC 15707 were incubated in a nitrogen-carbon dioxide atmosphere at $4^{\circ}C$ for 30 days. pH and acidity of all the samples were in the range of $pH\;6.6{\sim}pH\;6.9$ and $0.4%{\sim}0.55%$ for 30 days, respectively. The viable cell numbers in non-fat dry milk reached above $8.4{\times}10^9\;CFU/mL$ after 15 days. The glucose content in soymilk was $4.5{\times}10^{-2}{\sim}5.5{\times}10^{-2}\;mM$ at 10 days of storage. Milk and soymilk containing B. longum at $4^{\circ}C$ were found to be different in taste, odor, off-flavor at each storage time (0, 2, 4, 6, 8, and 10 days). Sensory scores indicated that milk containing bifidobacteria was poorly affected by the storage time, but milk at 4 days of storage was quite close in odor and off-flavor to milk with storage time 0. Soymilk containing bifidobacteria at 2 and 4 days of storage had significantly higher acceptability of taste than soymilk with storage time 0.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.5
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• pp.659-664
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• 2012

The present experiment aimed to evaluate the effect of HMBi on the production performance and metabolism in dairy cows. Thirty multiparous Holstein dairy cows under similar conditions were randomly assigned to three dietary treatments; i) Control, a basal diet; ii) T1, a basal diet plus HMBi (0 g prepartum and 18 g postpartum); and iii) T2, a basal diet plus HMBi (10 g prepartum and 18 g postpartum). Treatments were initiated 21 d before expected calving and continued through 91 d postpartum. HMBi was top-dressed onto the total mixed ration of each cow. Treatments did not affect dry matter intake, plasma urea nitrogen, peak milk yield, days to peak milk yield, nonesterified fatty acid, glutamate pyruvate transaminase, glutamic oxalaetic transaminase, milk fat content, milk protein content, milk lactose content, and milk solid non-fat content. The milk composition yields were increased by the HMBi-supplemented treatment. The T1 and T2 treatments increased the yields of 4% fat-corrected milk yield, milk fat, milk protein, and milk lactose compared with the control. Although there was no difference in the milk composition of the control and T2-treated cows, the T2-treated cows exhibited higher milk fat yield (increased by 74 g/d), lower milk urea nitrogen (reduced by 3.41%), and plasma ${\beta}$-hydroxy butyrate than the control cows. The results indicate that HMBi supplementation to diet has beneficial effects, and that there is no difference between supplementation at prepartum and starting only at parturition.

• Applied Biological Chemistry
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• v.28 no.4
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• pp.261-270
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• 1985

This study was carried out he effects of water activity on the nonenzymatic browning reactions of dry milk powders. Samples (Dry Whole Milk DWM, Nonfat Dry Milk NFDM) stored at $55^{\circ}C$ were analyzed for $O_2,\;CO_2$ and $H_2O$ by Gas chromatographic method. The brown color which developed from the Maillard reaction was also measured by a reflective spectrophotometer. And linear regression equations and the correlation coeffcients were determined for $O_2,\;CO_2$ and brown color vs. storage, and the results are as follows. The amount of brown and off-flavor development increase as the water activities increase about 0.44 Aw during storage. Both DWM and NFDM milk products show a plateau in water absorption between the 0.33 and 0.44 water activities. DWM produced more browning than NFDM which may be partially due to the carbonyl amine reaction. The $CaCl_2$ solution may exude a water activity lower than 0.44 and varies greatly with temperatures. Oxygen is depleted as brown color development.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.416-423
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• 1993

Milk powders were made by several drying methods and raw materials, and rheological properties of milk powders were investigated by an Instron Universial Testing Machine. The results obtained are summarised as follows. Bulk density of the drum dried whole milk powder was $0.382\;g/cm^3$ and was higher than that of other milk powders. The Hausner ratio, angle of repose and compressibility of the spray dried skim milk powder were 1.127, $32.5^{\circ}$ and 0.029 respectively. This result indicated that skim milk powder showed free flowing characterristics. The compressibility of the drum dried whole milk powder was decreased by the decrease of moisture content. Compressibility of the milk granule was 0.056 and was lower than that of milk powder. The spray dried milk powder of a grobular shape had higher solidity than the freeze or drum dried milk powder. The drum dried milk powder particles consist of irregular shape.