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Viability and Functions of Alginate-microencapsulated Islets Isolated from Neonatal Pigs

  • Lin, Yi-Juain;Wang, Jui-Ping;Chung, Yu-Tung;Sun, Yu-Ling;Chou, Yu-Chi
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.5
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    • pp.795-801
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    • 2007
  • Patients with Type I diabetes mellitus have been treated with porcine insulin for several decades and pigs have recently been deemed an ideal source of microencapsulated islet cells for clinical xenotransplantation. In this study, neonatal pigs were anesthetized and sacrificed prior to a pancreatectomy. Islet cells were isolated from pancreas via collagenase digestion. Islet cells were separated and collected by hand under microscopic guidance. These cells were suspended in 1.4% sodium alginate solution and encapsulated by dropping them into 1.1% calcium chloride solution and in which the round gel in size was 250-400 ${\mu}m$ in diameter. Viability of the microencapsulated islet cells cultured in medium at $37^{\circ}C$ was assessed by MTT assay. Furthermore, insulin released in response to glucose challenge was investigated using an enzyme-linked immunosorbent assay. Secretion of insulin was low in response to the basal glucose solution (4.4 mM) in medium and was significantly higher in response to the high glucose solution (16.7 mM). The viability of microencapsulated islet cells did not differ significantly over a period of 7 days; that is, the increasing pattern of insulin concentration in the culture medium after glucose stimulation interval day was similar throughout the 7 days cultivation. In summary, experimental evidences indicated that the effects of alginate-microencapsulation prolonged survival of the neonatal porcine islets in vitro cultures and the insulin response to glucose of the islets was maintained.

Influence of Energy Level and Glycine Supplementation on Performance, Nutrient Digestibility and Egg Quality in Laying Hens

  • Han, Yung-Keun;Thacker, Philip A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.10
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    • pp.1447-1455
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    • 2011
  • Sixty four, 30-week-old, Lohmann Brown-Lite laying hens were randomly allocated to one of four treatments with eight replicates per treatment and two hens per replicate for a 10 week study. The control diet was a high energy (11.81 MJ/kg) diet and the moderate energy (11.39 MJ/kg) diets were formulated dropping the level of animal fat. The three moderate energy diets were fed either unsupplemented (0.0%) or supplemented with 0.05 or 0.10% glycine. There were no significant differences (p>0.05) in hen-day egg production, egg weight, feed intake or feed conversion between birds fed the unsupplemented moderate energy and high energy diets. Significant differences were detected concerning egg components and quality measurements as assessed by albumen percentage (p = 0.02), yolk weight (p = 0.02), yolk percentage (p<0.01), yolk to albumen ratio (p<0.01) and yolk color (p = 0.01) between birds fed the unsupplemented moderate and high energy diets. Glycine supplementation of the moderate energy diet linearly increased (p<0.01) egg weight and feed intake with no significant (p>0.05) effects on egg production or feed conversion. Glycine supplementation significantly increased egg content (p<0.01), albumen weight (p<0.01) and percentage (p<0.01) as well as yolk weight (p<0.01) while yolk percentage (p = 0.04), yolk to albumen ratio (p = 0.01) and egg shell percentage (p<0.01) were linearly decreased. Supplementation with glycine produced a tendency (p = 0.09) towards an increase in the percentage of large eggs (63-72.9 g) produced with a concomitant decrease in the percentage of small (below 53 g) eggs (p = 0.09). The overall results of this study indicate that glycine supplementation of laying hen rations has the potential to increase egg production and weight. These increases appeared to be mediated through increases in feed intake and the ileal digestibility of fat and energy.

WLAN OFDMA Random Access Based Communications for In-Aircraft Wireless Sensor Networks (비행체 무선 센서망을 위한 무선랜 OFDMA 임의접근 기반 통신)

  • Ahn, Woojin;Kim, Ronny Yongho
    • Journal of Advanced Navigation Technology
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    • v.20 no.3
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    • pp.252-257
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    • 2016
  • In this paper a novel access scheme for uplink multiuser transmission based on IEEE 802.11ax random access in in-aircraft wireless sensor networks is proposed. The proposed scheme provides an efficient access control mechanism with three divisions of OFDMA backoff counter (OBO): access, deferring, dropping, which controls the number of potential uplink transmission stations. The proposed scheme can be used efficiently in in-aircraft wireless sensor network where a large number of sensors need to be supported. By using the proposed scheme, since in-aircraft sensors attempt channel access using the proposed differentiated OBO parameters, the number of stations exceeds the access capacity can be efficiently controlled. This paper also provides the mathematical analysis of the proposed scheme, regarding the optimal parameters. According to the performance analysis, the proposed scheme is able to efficiently control the access behavior of wireless sensors in the network.

Performance Comparison of Call Admission Control Based on Predictive Resource Reservations in Wireless Networks (무선망의 자원예측에 의한 호 수락제어방식의 성능비교)

  • Lee, Jin-Yi
    • Journal of Advanced Navigation Technology
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    • v.13 no.3
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    • pp.372-377
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    • 2009
  • This paper evaluates the performance of three methods for predicting resources requested by mobile's calls and a call admission algorithm based on these predicting methods respectively in wireless networks. The first method is based on Wiener prediction model and the second method is based on the MMOSPRED algorithm and the third method is based on the neural network. The proposed call admission algorithm is based on prioritized handoff call in resource allocation. The resources for future handoff calls are therefore predicted and reserved in advance, and then new calls are admitted as long as the remaining resources are sufficient. We compare their performances in terms of prediction error, new call blocking and handoff dropping probabilities by simulation. Results show that the CAC based on Wiener prediction model performs favorably due to exact resources prediction.

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Properties and Oxidation Stability of Fish Oil Capsules Manufactured with Calcium Alginate Gels (Calcium Alginate로 제조한 어유 캡슐의 성질 및 산화안정성)

  • Yun, Young-Soo;Jang, Su-Ji;Kim, Hong-Deok;Kim, Seon-Bong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.5
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    • pp.589-595
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    • 2015
  • Alginates are used to encapsulate various materials, including food, cosmetics, and pharmaceuticals. This study examined the properties and oxidation stability of fish oil capsules manufactured with calcium alginate gels. The fish oil capsules were manufactured by dropping sodium alginate solution and fish oil into a calcium chloride solution through nozzles. The membrane thickness, sphericity, rupture strength and deformation depth of the fish oil capsules were determined. The peroxide value of the fish oil was assayed to determine the oxidation stability of the capsules. The capsules measured approximately 3 mm with a membrane thickness of 90 μm independent of the amount of fish oil added. As the amount of fish oil encapsulated increased, the sphericity, rupture strength and deformation depth of the capsules decreased. The encapsulation efficiency increased until the amount of fish oil was 30%. The oxidation stability of fish oil in capsules was dependent on the type of nozzle, e.g., the oxidation stability of fish oil in capsules made using a double nozzle was greater than with a single nozzle. These results should lead to industrial application of fish oils including eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, as nutraceuticals.

ON THE SUFFICIENT CONDITION FOR THE LINEARIZED APPROXIMATION OF THE B$\"{E}$NARD CONVECTION PROBLEM

  • Song, Jong-Chul;Jeon, Chang-Ho
    • Bulletin of the Korean Mathematical Society
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    • v.29 no.1
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    • pp.125-135
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    • 1992
  • In various viscus flow problems it has been the custom to replace the convective derivative by the ordinary partial derivative in problems for which the data are small. In this paper we consider the Benard Convection problem with small data and compare the solution of this problem (assumed to exist) with that of the linearized system resulting from dropping the nonlinear terms in the expression for the convective derivative. The objective of the present work is to derive an estimate for the error introduced in neglecting the convective inertia terms. In fact, we derive an explicit bound for the L$_{2}$ error. Indeed, if the initial data are O(.epsilon.) where .epsilon. << 1, and the Rayleigh number is sufficiently small, we show that this error is bounded by the product of a term of O(.epsilon.$^{2}$) times a decaying exponential in time. The results of the present paper then give a justification for linearizing the Benard Convection problem. We remark that although our results are derived for classical solutions, extensions to appropriately defined weak solutions are obvious. Throughout this paper we will make use of a comma to denote partial differentiation and adopt the summation convention of summing over repeated indices (in a term of an expression) from one to three. As reference to work of continuous dependence on modelling and initial data, we mention the papers of Payne and Sather [8], Ames [2] Adelson [1], Bennett [3], Payne et al. [9], and Song [11,12,13,14]. Also, a similar analysis of a micropolar fluid problem backward in time (an ill-posed problem) was given by Payne and Straughan [10] and Payne [7].

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The Effects of Dietary Iodine Intake on the Postpartum Thyroiditis(PPT) Manifestation (산모의 요오드섭취가 산후 갑상선염 발현에 미치는 영향)

  • 조여원
    • Journal of Nutrition and Health
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    • v.30 no.10
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    • pp.1195-1202
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    • 1997
  • Iodine-rich seaweed soup has been traditionally supplied to postpartum women in Korea. This dietary habit might introduce over-intake of iodine above the recommended requirements, and might provoke postpartum thyroid dysfunction. Although the response to excess iodine intake is highly variable, goiter, hyperthyroidism, hypothyroidism, and thyroiditis could follow the daily intake of 1,500$\mu\textrm{g}$ of iodine. A few studies are available concerning iodine toxicity in Korea. The purpose of this study was to investigate the relationships between the dietary intake of iodine and thyroid function change as well as the incidence of postpartum thyroiditis. One hundred and thirty-seven postpartum women who had experienced normal deliveries were studied. Dietary intake of iodine and excretion concentration of iodine in breast milk and maternal urine were measured . Serum T$_3$, T$_4$, TSH, anti-thyroglobulin antibody, and anti-microsomal antibody were anlayzed 1 week before delivery and 1, 6, 12, and24 weeks after delivery. Iodine intake was analyzed by one-to-one interviews using 24-hr recall and a food frequncy questionnaire. The result showed that the intake of dietary iodine before delivery and 1 and24 weeks after delivery were 483$\mu\textrm{g}$/day, 3367$\mu\textrm{g}$/day, and 1069$\mu\textrm{g}$/day, respectively. The concentration of iodine in urine at the first week after delivery was 63$\mu\textrm{g}$/dL, and 23.9$\mu\textrm{g}$/dL in breast milk . The levels of serum T$_3$ and T$_4$ before delivery were 2.01ng/mL and 11.49$\mu\textrm{g}$U/dL, respectively, showing that the levels were gradually dropping to normal values after delivery. Positive serum anti-thyroglobulin antibody and anti-microsomal antibody appeared in 3 cases. After a 24 week follow-up period , 6 women(10.3%) experienced cases of postpartum thyroiditis, 5 of which were cases of hyperthyroidism and one of which was a case of hypothyroidism. These figures of postpartum thyroiditis are similar to those of other countries.

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Markov CAC model in Wireless Mobile Networks Using AMC (AMC를 사용하는 무선 이동 네트워크에서 Markov CAC 모델)

  • Kwon Eun-Hyun;Park Hyo-Soon;Lee Jai-Yong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.4B
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    • pp.270-277
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    • 2006
  • In the wireless adaptive modulation and coding(AMC) systems, the modulation type of user's connection can be changed dynamically. and the ongoing connection might fail due to the change of modulation. In this paper, we approach the AMC-induced CAC problem by focusing on the guaranteed connection. Three kinds of calls, new, handoff, and modulation-changed calls, are considered. We propose a modified guard channel CAC scheme that allows the modulation-changed and handoff calls to use the guard channel. Then we analyze a Markov model for the CAC scheme with long-term AMC in mind. According to the simulation results, the proposed approach reduces the call dropping probability for modulation-changed calls, which suggests the threshold of guard channels can be determined based on the proposed approach.

Performance Analysis of Joint Call Admission Control(JCAC) According to Resource Reservation Rate Changes in LTE-WLAN Heterogeneous Network Environment (LTE-WLAN 이종 네트워크 환경에서 자원예약률 변화에 따른 통합 호 수락 제어의 성능분석)

  • Kim, Yi-Kang;Kim, Seung-Yeon;Ryu, Seung-Wan;Cho, Choong-Ho
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.5A
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    • pp.473-484
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    • 2011
  • In this paper, we analyze and propose the Joint Call Admission Control(JCAC) scheme to combine network selection scheme and radio resource reservation based Call Admission Control(CAC) in LTE-WLAN heterogeneous networks. First, We propose the JCAC system that uses network decision rate to select a network for terminal and radio resource reservation scheme in overlaying LTE-WLAN network environment. And we analyze the performance of a proposed system using markov chain model. The performance is presented in terms of the new call blocking probability, handoff call dropping probability, and channel utilization of each network. As a performance result of the our research, the system using JCAC is better than the system using non-JCAC. We found a suitable resource reservation rate that is 10% in the system using JCAC. Our work may be useful as a guideline of resource reservation rate to introduce JCAC system using resource reservation scheme in overlaying LTE-WLAN network environment.

A study on the PWM(pulse witdh modulation) current source Inverter with utility (태양광발전 연계 시스템에 의한 PWM 전류형 인버어터에 관한 연구)

  • Hwang, Lak-Hoon;Choi, Ho-Kyu;Sin, Yang-Ho;Lee, Chun-Sang;Kim, Ju-Rae;Jo, Sang-Rou;Jo, Moon-Taeck
    • Proceedings of the KIEE Conference
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    • 2001.07b
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    • pp.1020-1022
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    • 2001
  • because the output of solar cell is direct, it is necessary to install D/A converter system for A.C load, and in case of driving utility line system, it is possible to drive system relation when the system supplies sinusodal current ant voltage having unit power factor. As the characteristics of the soar cell output the is influenced by dailysunight charge, for more electric power it is essential to control the direction toward the san so that the driving point of solar cell can always operate near maximum output point. PWM modulation device among electric power converters must have stable modulation at anytime when it includes noise-factors such as noise-wave and noises on electric voltage wave, a synchronous signal system. In dealing with synchronous signal for control and control signal by microprocessor, it is necessary to compensate it because there is time difference between sample paint and carrier wave. On this papers, single phase PWM current type invertor controled the solar cell having typical voltage dropping character has optimun short current in short, reduces D.C reactance, composes controller for modulation and keeps lower harmonic and high power factor keeping maximum output of solar cell according daily sunlight charge variation.

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