• Mycobiology
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• v.46 no.4
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• pp.429-439
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• 2018

To develop a convenient promoter analysis system for fungi, a null-pigment mutant (NPG) of Aspergillus nidulans was used with the 4'-phosphopantetheinyl transferase (PPTase) gene, npgA, which restores the normal pigmentation in A. nidulans, as a new reporter gene. The functional organization of serially deleted promoter regions of the A. nidulans trpC gene and the Cryphonectria parasitica crp gene in filamentous fungi was representatively investigated to establish a novel fungal promoter assay system that depends on color complementation of the NPG mutant with the PPTase npgA gene. Several promoter regions of the trpC and crp genes were fused to the npgA gene containing the 1,034-bp open reading frame and the 966-bp 3' downstream region from the TAA, and the constructed fusions were introduced into the NPG mutant in A. nidulans to evaluate color recovery due to the transcriptional activity of the sequence elements. Serial deletion of the trpC and crp promoter regions in this PPTase reporter assay system reaffirmed results in previous reports by using the fungal transformation step without a laborious verification process. This approach suggests a more rapid and convenient system than conventional analyses for fungal gene expression studies.

• Journal of Information Processing Systems
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• v.17 no.1
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• pp.136-150
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• 2021

The modern market is highly competitive. It has progressed from traditional competition between enterprises to competition between supply chains. To ensure that enterprise can form the best strategy consistently, it is necessary to evaluate the trust of other enterprises in the supply chain. First, this paper analyzes the background and significance of supply chain trust research, analyzes and expounds on the qualitative and quantitative methods of supply chain trust evaluation, and summarizes the research in this field. Analytic hierarchy process (AHP) is the most frequently used method in the literature to evaluate and rank criteria through data analysis. However, the input data for AHP analysis is based on human judgment, and hence there is every possibility that the data may be vague to some extent. Therefore, in view of the above problems, this study improves the global trust method based on chain iteration. The improved global trust evaluation method based on chain iteration is more flexible and practical, hence, it can more accurately evaluate supply chain trust. Finally, combined with an actual case of Zhaoxian Chengji Food Co. Ltd., the paper qualitatively analyzes the current situation of supply chain trust management and effectively strengthens the supervision of enterprises to cooperative enterprises. Thus, the company can identify problems on time and strategic adjustments can be implemented accordingly. The effectiveness of the evaluation method proposed in this paper is demonstrated through a quantitative evaluation of its trust in downstream enterprise A. Results suggest that the subjective preferences of and historical transactions together affect the final evaluation of trust.

• International Journal of Naval Architecture and Ocean Engineering
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• v.13 no.1
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• pp.628-640
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• 2021

To investigate the supercavity geometry and gas flow structure for the supercavities with two closure types under the different flow conditions, an inhomogeneous multiphase model with the SST turbulence model was established, and validated by experimental results. The results show that two distinct regions exist inside the supercavity, which include the downstream flow region along the gas-water interface and the reverse flow region. For the twin-vortex supercavity, the internal gas leaks from the supercavity boundary by two paths: the supercavity surface and the two-vortex tubes. Increasing Froude number leads to more internal gas stripped from the supercavity surface. Two types of gas loss exist for the re-entrant jet supercavity with high Froude number, one type is the steady process of gas loss, and the major gas-leaking path is the supercavity surface rather than supercavity closure region. The other type is the unsteady periodic ejection, and the gas cluster of periodic ejection is merely a small part of the gas stored inside the supercavity.

• Nuclear Engineering and Technology
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• v.52 no.12
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• pp.2928-2938
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• 2020

A light water nuclear Reactor has been exergy analyzed, and the rate of irreversible exergy loss and exergy destruction is calculated for each of its components. The ratio of these losses compared to the total input exergy loss is calculated, which shows that most irreversible losses occur in the reactors, turbines, steam generators, respectively, as well as in the downstream operations. The main aim of this paper is to optimize the power plant using an innovative firefly algorithm and then to propose a novel strategy to improve the overall performance of the plant. As shown in the results, the exergy destruction rate of the plant decreased by 1.18% using the firefly method, and the exergy efficiency of the plant reached 29.3% comparing to the operational amount of 28.99%. Also, the results of the firefly optimization process compared to the Genetic algorithm and gravitational search algorithm to study the accuracy of the model for exergy analysis fitness problems in the power plants and the results of this comparison has shown that the results are nearly similar in the mentioned methods. However, the firefly is faster and more accurate in limited iterations.

• Journal of The Korean Society of Agricultural Engineers
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• v.65 no.6
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• pp.37-49
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• 2023

Rainfall data is one of the most important data in hydrologic modeling. In this study, the impacts of spatial resolution of precipitation data on hydrological responses were assessed using SWAT in the Santa Fe River Basin, Florida. High correlations were found between the FAWN and NLDAS rainfall data, which are observed weather data and simulated weather data based on observed data, respectively. FAWN-based scenarios had higher maximum rainfall and more rainfall days and events compared to NLDAS-based scenarios. Downstream areas showed lower correlations between rainfall and peak discharge than upstream areas due to the characteristics of study site. All scenarios did not show significant differences in base flow, and showed less than 5% of differences in high flows among NLDAS-based scenarios. The impact of resolution will appear differently depending on the characteristics of the watershed and topography and the applied model, and thus, is a process that must be considered in advance in runoff simulation research. The study suggests that applying the research method to watersheds in Korea may yield more pronounced results, and highlights the importance of considering data resolution in hydrologic modeling.

• Journal of Microbiology and Biotechnology
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• v.33 no.10
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• pp.1268-1280
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• 2023

Echinacoside (ECH) is a naturally occurring phenylethanoid glycoside, isolated from Echinacea angustifolia, and this study aimed to analyze its effect on thermogenesis and its interaction with dopaminergic receptors 1 and 5 (DRD1 and DRD5) in 3T3-L1 white adipocytes and mice models. We employed RT-PCR, immunoblot, immunofluorescence, a staining method, and an assay kit to determine its impact. ECH showed a substantial increase in browning signals in vitro and a decrease in adipogenic signals in vivo. Additionally, analysis of the iWAT showed that the key genes involved in beiging, mitochondrial biogenesis, and ATP-dependent thermogenesis were upregulated while adipogenesis and lipogenesis genes were downregulated. OXPHOS complexes, Ca²⁺ signaling proteins as well as intracellular Ca²⁺ levelswere also upregulated in 3T3-L1 adipocytes following ECH treatment. This was collectively explained by mechanistic studies which showed that ECH mediated the beiging process via the DRD1/5-cAMP-PKA and subsequent downstream molecules, whereas it co-mediated the α1-AR-signaling thermogenesis via the DRD1/5/SERCA2b/RyR2/CKmt pathway in 3T3-L1 adipocytes. Animal experiments revealed that there was a 12.28% reduction in body weight gain after the ECH treatment for six weeks. The effects of ECH treatment on adipose tissue can offer more insights into the treatment of obesity and metabolic syndrome.

• Korea Trade Review
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• v.46 no.4
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• pp.205-217
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• 2021

This paper examines the effects of intellectual property protection policies on upstream firms' R&D investments in order to derive policy implications in relation to the fostering of the intermediate goods industry. To that end, the dependence on import of intermediate goods and the degree of protection of intellectual property rights are introduced into the model to analyze the effects of R&D investments on the dependence on imports and the effects of intellectual property rights protection policies on the level of R&D investments in order and the social welfare effects are also checked. The policy implications derived in this paper, which used an oligopolistic market model with a vertical specialization structure, are as follows. As R&D investments expand, upstream firm begins to have price competitiveness, the dependence on import of intermediate goods by downstream firm decreases, and social welfare increases. That is, in order to strengthen the independence of the intermediate goods industry, R&D investments by upstream firm should be expanded, and to promote this, the government should strengthen the protection of intellectual property rights.

• ETRI Journal
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• v.46 no.2
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• pp.277-289
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• 2024

In this paper, we describe a neural network-based application that recommends multiple items using dialog context input and simultaneously outputs a response sentence. Further, we describe a multi-item recommendation by specifying it as a set of clothing recommendations. For this, a multimodal fusion approach that can process both cloth-related text and images is required. We also examine achieving the requirements of downstream models using a pretrained language model. Moreover, we propose a gate-based multimodal fusion and multiprompt learning based on a pretrained language model. Specifically, we propose an automatic evaluation technique to solve the one-to-many mapping problem of multi-item recommendations. A fashion-domain multimodal dataset based on Koreans is constructed and tested. Various experimental environment settings are verified using an automatic evaluation method. The results show that our proposed method can be used to obtain confidence scores for multi-item recommendation results, which is different from traditional accuracy evaluation.

• Membrane Journal
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• v.34 no.2
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• pp.124-131
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• 2024

Viruses have various applications in the biopharmaceutical industry. They are used in pesticide production, production of vaccines, gene transfers, cancer therapeutics, and more. The downstream processing of viruses is an essential step for their biological and pharmaceutical applications. Among the various processes, the purification of viruses is critical. Membrane chromatography plays a vital role in this process. While ion exchange membrane chromatography is a primarily used method, it has various limitations regarding size exclusion and insufficient purification. Also, it cannot be applied to the rapidly changing strains of viruses such as influenza. This review examines various improved methods of membrane chromatography or alternatives. It focuses on purification, viral recovery rates, and scalability of the methods.

• The Plant Pathology Journal
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• v.40 no.3
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• pp.235-250
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• 2024

During the infection process, plant pathogenic fungi encounter plant-derived oxidative stress, and an appropriate response to this stress is crucial to their survival and establishment of the disease. Plant pathogenic fungi have evolved several mechanisms to eliminate oxidants from the external environment and maintain cellular redox homeostasis. When oxidative stress is perceived, various signaling transduction pathways are triggered and activate the downstream genes responsible for the oxidative stress response. Despite extensive research on antioxidant systems and their regulatory mechanisms in plant pathogenic fungi, the specific functions of individual antioxidants and their impacts on pathogenicity have not recently been systematically summarized. Therefore, our objective is to consolidate previous research on the antioxidant systems of plant pathogenic fungi. In this review, we explore the plant immune responses during fungal infection, with a focus on the generation and function of reactive oxygen species. Furthermore, we delve into the three antioxidant systems, summarizing their functions and regulatory mechanisms involved in oxidative stress response. This comprehensive review provides an integrated overview of the antioxidant mechanisms within plant pathogenic fungi, revealing how the oxidative stress response contributes to their pathogenicity.