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Virus Purification by Membrane Chromatography: A Review

멤브레인 크로마토그래피에 의한 바이러스 정제 : 리뷰

  • Gayatri Bhamidipatia (Life Science and Biotechnology Department, Underwood Division, Underwood International College, Yonsei University) ;
  • Rajkumar Patel (Energy and Environmental Science and Engineering, Integrated Science and Engineering Division, Underwood International College, Yonsei University)
  • Received : 2023.12.10
  • Accepted : 2024.04.12
  • Published : 2024.04.30

Abstract

Viruses have various applications in the biopharmaceutical industry. They are used in pesticide production, production of vaccines, gene transfers, cancer therapeutics, and more. The downstream processing of viruses is an essential step for their biological and pharmaceutical applications. Among the various processes, the purification of viruses is critical. Membrane chromatography plays a vital role in this process. While ion exchange membrane chromatography is a primarily used method, it has various limitations regarding size exclusion and insufficient purification. Also, it cannot be applied to the rapidly changing strains of viruses such as influenza. This review examines various improved methods of membrane chromatography or alternatives. It focuses on purification, viral recovery rates, and scalability of the methods.

바이러스는 생물 의약 산업에서 다양한 응용 분야를 가지고 있다. 그들은 살충제 생산, 백신 생산, 유전자 전달, 암 치료제 등에 사용된다. 바이러스의 하류 처리는 그들의 생물학적 및 의약적 응용을 위한 필수 단계이다. 다양한 과정 중에서 바이러스의 정제는 매우 중요하다. 막 크로마토그래피는 이 과정에서 중요한 역할을 한다. 이온 교환 막 크로마토그래피는 주로 사용되는 방법이지만 크기 배제 및 불충분한 정제에 관한 다양한 제한을 가지고 있다. 또한, 이는 인플루엔자와 같은 빠르게 변화하는 바이러스의 균주에 적용될 수 없다. 이 검토는 막 크로마토그래피의 다양한 개선된 방법 또는 대안을 검토한다. 이는 정제, 바이러스 회수율 및 방법의 확장성에 초점을 맞추고 있다.

Keywords

References

  1. J. Chen, B. Yu, H. Cong, and Y. Shen, "Recent development and application of membrane chromatography", Anal. Bioanal. Chem., 415, 45 (2023).
  2. P. Kosiol, M. T. Muller, B. Schneider, B. Hansmann, V. Thom, and M. Ulbricht, "Determination of pore size gradients of virus filtration membranes using gold nanoparticles and their relation to fouling with protein containing feed streams", J. Membr. Sci., 548, 598 (2018).
  3. M. Mertz and K. Castiglione, "Increased protein encapsulation in polymersomes with hydrophobic membrane anchoring peptides in a scalable process", Int. J. Mol. Sci., 22, 7134 (2021).
  4. J. Fan, E. Barbieri, S. Shastry, S. Menegatti, C. Boi, and R. G. Carbonell, "Purification of adeno-associated virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes", Membr., 12, 944 (2022).
  5. K. Kawka, A. N. Wilton, E. J. Redmond, M. F. C. Medina, B. D. Lichty, R. Ghosh, and D. R. Latulippe, "Comparison of the performance of anion exchange membrane materials for adenovirus purification using laterally-fed membrane chromatography", Biochem. Eng. J., 182, 108417 (2022).
  6. K. O'Donnell, S. Krishnathu, R. Bhatia, Z. Huang, and W. Kelly, "Evaluation of two-species binding model with anion-exchange membrane chromatography to predict pressure buildup during recovery of virus", Chem. Eng. Sci., 237, 116535 (2021).
  7. K. Kawka, P. Madadkar, U. Umatheva, S. Shoaebargh, M. F. C. Medina, B. D. Lichty, R. Ghosh, and D. R. Latulippe, "Purification of therapeutic adenoviruses using laterally-fed membrane chromatography", J. Membr. Sci., 579, 351 (2019).
  8. K. Kawka, A. N. Wilton, P. Madadkar, M. F. C. Medina, B. D. Lichty, R. Ghosh, and D. R. Latulippe, "Integrated development of enzymatic DNA digestion and membrane chromatography processes for the purification of therapeutic adenoviruses", Sep. Purif. Technol., 254, 117503 (2021).
  9. S. B. Carvalho, R. J. S. Silva, M. G. Moleirinho, B. Cunha, A. S. Moreira, A. Xenopoulos, P. M. Alves, M. J. T. Carrondo, and C. Peixoto, "Membrane-based approach for the downstream processing of influenza virus-like particles", Biotechnol. J., 14, 1800570 (2019).
  10. A. R. Fortuna, S. van Teeffelen, A. Ley, L. M. Fischer, F. Taft, Y. Genzel, L. Villain, M. W. Wolff, and U. Reichl, "Use of sulfated cellulose membrane adsorbers for chromatographic purification of cell cultured-derived influenza A and B viruses", Sep. Purif. Technol., 226, 350 (2019).
  11. Z. Yang, X. Xu, C. A. T. Silva, O. Farnos, A. Venereo-Sanchez, C. Toussaint, S. Dash, I. Gonzalez-Dominguez, A. Bernier, O. Henry, and A. Kamen, "Membrane chromatography-based downstream processing for cell-culture produced influenza vaccines", Vaccines, 10, 1310 (2022).
  12. C. J. Chung, A. L. Grimm, C. L. Wilson, U. B. R. Balasuriya, G. Chung, P. J. Timoney, C. B. Bandaranayaka-Mudiyanselage, S. S. Lee, and T. C. McGuire, "Enhanced sensitivity of an antibody competitive blocking enzyme-linked immunosorbent assay using Equine arteritis virus purified by anion-exchange membrane chromatography", J. Vet. Diagn. Invest., 27, 728 (2015).
  13. T. A. Grein, R. Michalsky, M. Vega Lopez, and P. Czermak, "Purification of a recombinant baculovirus of Autographa californica M nucleopolyhedrovirus by ion exchange membrane chromatography", J. Virol. Methods, 183, 117 (2012).
  14. C. Ladd Effio, T. Hahn, J. Seiler, S. A. Oelmeier, I. Asen, C. Silberer, L. Villain, and J. Hubbuch, "Modeling and simulation of anion-exchange membrane chromatography for purification of Sf9 insect cell-derived virus-like particles", J. Chromatogr. A, 1429, 142 (2016).
  15. K. Lothert, G. Sprick, F. Beyer, G. Lauria, P. Czermak, and M. W. Wolff, "Membrane-based steric exclusion chromatography for the purification of a recombinant baculovirus and its application for cell therapy", J. Virol. Methods, 275, 113756 (2020).
  16. Y. Masuda, Y. Ogino, K. Yamaichi, Y. Takahashi, K. Nonaka, and K. Wakamatsu, "The prevention of an anomalous chromatographic behavior and the resulting successful removal of viruses from monoclonal antibody with an asymmetric charge distribution by using a membrane adsorber in highly efficient, anion-exchange chromatography in flowthrough mode", Biotechnol. Prog., 36, e2955 (2020).
  17. D. J. McNally, D. Darling, F. Farzaneh, P. R. Levison, and N. K. H. Slater, "Optimised concentration and purification of retroviruses using membrane chromatography", J. Chromatogr. A, 1340, 24 (2014).
  18. S. Rajesh, C. Crandall, S. Schneiderman, and T. J. Menkhaus, "Cellulose-graft-polyethyleneamidoamine anion-exchange nanofiber membranes for simultaneous protein adsorption and virus filtration", ACS Appl. Nano Mat., 1, 3321 (2018).
  19. A. R. Fortuna, F. Taft, L. Villain, M. W. Wolff, and U. Reichl, "Continuous purification of influenza A virus particles using pseudo-affinity membrane chromatography", J. Biotechnol., 342, 139 (2021).
  20. P. Marichal-Gallardo, M. M. Pieler, M. W. Wolff, and U. Reichl, "Steric exclusion chromatography for purification of cell culture-derived influenza A virus using regenerated cellulose membranes and polyethylene glycol", J. Chromatogr. A, 1483, 110 (2017).
  21. A. L. Hejmowski, K. Boenning, J. Huato, A. Kavara, and M. Schofield, "Novel anion exchange membrane chromatography method for the separation of empty and full adeno-associated virus", Biotechnol. J., 17, 2100219 (2021).