• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
• /
• pp.96-96
• /
• 2003

Previously, wogonin (5,7-dihydroxy-8-methoxyflavone) was found to suppress proinflammatory enzyme expression including cyclooxygenase-2 (COX-2), contributing to in vivo anti-inflammatory activity against skin inflammation. However, the detailed effect on each skin cell type has not been understood. Therefore, present investigation was carried out to find the effect of wogonin on inflammation-associated gene expression from skin fibroblasts in culture using reverse transcriptase-polymerase chain reaction. As a result, it was found that wogonin (10 - 100 ${\mu}$M) clearly down-regulated COX -2 expression from NIH/3T3 cells treated with 12-O-tetradecanoylphorbol 13-acetate, interleukin-1${\beta}$ or tumor necrosis factor-a. But, the expression levels of COX-1, interleukin-1${\beta}$ and fibronectin were not significantly affected. This finding was well correlated with significant reduction of prostaglandin E$_2$(PGE$_2$) production by wogonin. As a comparison, NS-398 (selective cyclooxygenase-2 inhibitor) did not suppress COX -2 expression and other gene levels, while PGE$_2$production was potently reduced at 0.1 - 10 ${\mu}$M. All these results suggest that COX -2 down-regulation of skin fibroblasts may be, at least in part, one of anti-inflammatory mechanisms of wogonin against skin inflammation.

• Molecular & Cellular Toxicology
• /
• 제1권2호
• /
• pp.124-129
• /
• 2005

Pro-inflammatory cytokines, such as tumor necrosis factor $(TNF)-{\alpha}$, interleukin-12 (IL-12) and interleukin $(IL-1)-{\beta}$, play a key role in causing inflammatory diseases, which are rheumatoid arthritis, Crohn's disease and sepsis. Accumulating evidences suggest that low level laser irradiation (LLLI) may have an anti-inflammatory action. However, there are few data regarding down regulation of Th1 immune response by using the diod typed laser emitting device for human patients. As a fundamental step in order to address this issue, we investigated immunological impact of the low level laser irradiation (10 mw laser diode with a wavelength of 630 nm) on expression of pro-inflammatory cytokines in murine immunocytes (splenocytes and peritoneal macrophages) in vitro. The LLLI on lipopolysaccharide (LPS 100 ng/ml)-stimulated murine splenocytes and macrophages, clearly down regulated mRNA expression of $TNF-{\alpha}$ and IL-12 in dose-dependent manner. In addition, LLLI significantly inhibits the NO production in the LPS-stimulated murine macrophages. This data suggests that LLLI (wavelength of 630 nm) may exert an anti-inflammatory action via modulation of pro-inflammatory cytokine and NO production pathway.

• Archives of Pharmacal Research
• /
• 제25권3호
• /
• pp.329-335
• /
• 2002

Previously, several prenylated flavonoids having a C-8 lavandulyl moiety were found to inhibit cyclooxygenase-1 (COX-1) as well as 5-lipoxygenase (5-LOX), and sophoraflavanone G was the most potent inhibitor against these eicosanoid generating enzymes among 19 prenylated flavonoids tested. In this investigation, effects of sophoraflavanone G on COX-2 induction from RAW 264.7 cells and in vivo inflammatory response were studied. Sophoraflavanone G inhibited prostaglandin $E_2{\;}(PGE_2)$ production from lipopolysaccharide (LPS)-treated RAW cells by COX-2 down-regulation at 1-50 uM. Other prenylated flavonoids including kuraridin and sanggenon D also down-regulated COX-2 induction at 10-25 uM, while kurarinone and echinoisoflavanone did not. In addition, sophoraflavanone G showed in vivo anti-inflammatory activity against mouse croton oil-induced ear edema and rat carrageenan paw edema via oral (2-250 mg/kg) or topical administration (10-250 ug/ear). Although the potencies of inhibition were far less than that of a reference drug, prednisolone, this compound showed higher anti-inflammatory activity when applied topically, suggesting a potential use for several eicosanoidrelated skin inflammation such as atopic dermatitis.

• 전력전자학회논문지
• /
• 제8권5호
• /
• pp.463-467
• /
• 2003

본 논문에서는 교류쵸퍼와 직렬보상변압기로 구성되는 교류전압제어장치에 대하여 연구한다. 먼저, 교류쵸퍼와 직렬보상변압기로 구성되는 기본적인 교류전압제어장치로 출력전압을 입력전압에서 10-20%정도 강압할 수 있는 형태의 강압형 교류전압 제어장치를 제안하고 기본동작원리 및 이의 안전한 운전을 위한 PWM 발생과 초기시퀀스로직을 제시하였다. 또한 이로부터 입력전압변동에 무관하게 일정 출력전압을 발생시키기 위한 자동전압제어장치(AVR: Automatic Voltage Regulator)로 사용가능한 두가지 형태의 직렬보상형 교류전압제어장치를 제안하고 동작원리를 기술하였다. 특히, 입력전압과 반대방향의 극성의 전압을 발생시킬 수 있는 새로운 형태의 양방향 교류쵸퍼를 제안하고, 이의 안전한 운전을 위한 PWM 발생을 위한 스위칭 패턴을 제시하였다. 제안된 교류전압제어회로의 경우 다양한 실험을 통하여 전력절감을 목적으로 하는 강압형 교류전압제어장치와 자동전압조절기의 용도로 효과적으로 적용될 수 있음을 확인하였다.

• 동의생리병리학회지
• /
• 제19권4호
• /
• pp.938-944
• /
• 2005

Down-regulation of melanin synthesis is required for recovery of pigmentary disorders and it is known that direct inhibitors of tyrosinase suppress melanin synthesis. We screened several oriental medicinal plants using B16/Fl0 cells and found that the methanolic extract of Cnidii Rhizoma down-regulated melanin synthesis effectively. Although the proliferation of Bl6/Fl0 cells was decresed by the methanolic extract of Cnidii Rhizoma, it did not appear necrosis. Bl6/F10 cells incubated with the methanolic extract of Cnidii Rhizoma showed reduced pigmentation and tyrosinase activity. Western blotting revealed that the amount of tyrosinase was decreased by the methanolic extract of Cnidii Rhizoma. These results suggest that the inhibitary effect of the methanolic extract of Cnidii Rhizoma on melanogenesis is due to the suppression of tyrosinase in Bl6/F10 cells and Cnidii Rhizoma is a candidate for an efficient whitening agent.

• 한국자동차공학회논문집
• /
• 제11권2호
• /
• pp.182-189
• /
• 2003

We have found that the damage of the front part for a vehicle and that of the rear part for a vehicle are the majority of frequency experienced by the traffic accidents. In conventional bumper system was designed by safety standard regulation at low speed crash. For example there are 2.5 mile and 5 mile bumper. The conventional bumper system was the crash from max 5.5 mile to 3 mile low speed occurs most frequently and results in the highest rate of repairing cost in statistically. On this study, in order to check the damageability and repairability of gas tube bumper system RCAR 15 km/h 40 % offset frontal crash test was adopted in low speed and we have a gas tube bumper parts test and vehicle test with gas tube bumper, we can find gas tube bumper system definitely can improve the damageability and repairability of the vehicles and contribute to down the repairing cost.

• Bulletin of the Korean Chemical Society
• /
• 제35권10호
• /
• pp.2985-2989
• /
• 2014

Autophagy is a self-digestion process in which intracellular structures are degraded in response to stress. Notably, prolonged autophagy leads to cell death. In this study, we investigated whether CX-4945, an orally available protein kinase 2 (CK2) inhibitor, induces autophagic cell death in human cervical cancer-derived HeLa cells and in human prostate cancer-derived LNCaP cells. CX-4945 treatment of both cell lines resulted in the formation of autophagosomes, in the conversion of microtubule-associated protein 1 light chain 3 (LC3), and in down-regulation of the Akt-mammalian target of rapamycin (mTOR)-p70 ribosomal protein S6 kinase (S6K) signaling cascade. Thus, pharmacologic inhibition of CK2 by CX-4945 induced autophagic cell death in human cancer cells by down-regulating Akt-mTOR-S6K. These results suggest that autophagy-inducing agents have potential as anti-cancer drugs.

• 대한의생명과학회지
• /
• 제26권2호
• /
• pp.57-65
• /
• 2020

Excessive drinking of alcohol is known to be one of the main causes of various neurological diseases, such as Alzheimer's disease. Scopoletin is known to have anti-inflammatory and antioxidative properties, and to protect nerve cells. This study examined whether scopoletin inhibits the alcohol-induced apoptosis of primary hippocampal neurons, and how scopoletin regulates several factors associated with the caspase-mediated pathway. To achieve this, the cell viability and apoptosis rate of primary hippocampal neurons were measured by Cell Counting Kit-8 and flow cytometry, respectively. Apoptosis-related protein expressions (Bax, Bid, caspase-3, caspase-9, and Poly (ADP-ribose) polymerase (PARP)) were analyzed by Western blotting, and the ANOVA method was used to confirm the significance of the measured results. As a result, scopoletin inhibited the expressions of alcohol-induced apoptosis and apoptosis-related proteins in primary hippocampal neurons. These results suggest that down-regulation of Bid, Bax, and cleaved caspase-9 expression induced by scopoletin down-regulates the expression of cleaved caspase-3, inhibits the expression of cleaved PARP, and finally, inhibits mitochondrial apoptotic pathways. The study suggests that scopoletin is worth developing as a candidate for neuroprotective agent.

• 한국정보통신학회:학술대회논문집
• /
• 한국해양정보통신학회 2005년도 춘계종합학술대회
• /
• pp.273-276
• /
• 2005

This paper presents an electronic ballast using a step down converter, a low frequency inverter for high pressure discharge lamp. The proposed ballast is composed of a full-wave rectifier, a step down converter operated as a current source with power regulation and a low frequency inverter with ignition circuit. The ignition circuit generates high voltage pulse of 1${\sim}$2[kV] peak, 130[Hz]. Moreover, it is able to reignite at regular intervals by protective circuit. As experimental results on the test, lamp voltage, current and consumption power are measured 132.5[V], 7.6[A] and 1,005[W], respectively. It was confirmed that the designed ballast operate the lamp with a constant power.

• 한국식품과학회지
• /
• 제39권2호
• /
• pp.181-188
• /
• 2007

8주령의 F1B golden Syrian 햄스터에게 동맥경화 유발식이와 함께 녹차 추출물을 각각 500 혹은 1,000 mg/kg b.w.의 양을 매일 경구투여하면서 6주간 사육하였을 때 햄스터 체내의 지질대사와 대동맥 내에서의 지방의 축적 정도에 미치는 영향을 조사하였다. 실험 결과 녹차추출물의 경구투여는 동맥경화유발식이를 섭취하는 햄스터의 혈중 중성지방과 총콜레스테롤치를 농도의존적으로 감소시켰고, 대동맥궁내에서의 지방의 축적을 예방하였다. 특히 녹차추출물 1,000 mg/kg b.w. 투여는 동맥경화유발식이를 섭취한 햄스터에서 간장내의 LDL receptor mRNA level을 증가시키는 경향을 보였다. 이러한 결과로 볼 때 녹차 추출물의 투여는 동맥경화유발식이를 섭취한 햄스터의 혈중 콜레스테롤치를 감소시키고 LDL receptor의 발현을 증가시킴으로써 동맥경화를 예방할 수 있음을 보여주었다.