Molecular & Cellular Toxicology

  • 제1권2호
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  • Pages.124-129
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  • 2005
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  • 1738-642X(pISSN)
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  • 2092-8467(eISSN)
대한독성유전 단백체학회 (The Korean Society of Toxicogenomics and Toxicoproteomics)
권호 표지

Anti Inflammatory Effect of Low Level Laser Irradiation on the LPS-stimulated Murine Immunocytes

  • Jin, Dan (Department of Microbiology, Institute of Basic Medical Science, Wonju Colleg eof Medicine, Yonsei University) ;
  • Lee, Jong-Young (Department of Radiation Oncology Wonju College of Medicine, Yonsei University) ;
  • Cho, Hyun-Chul (Department of Microbiology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Kim, Soo-Ki (Department of Microbiology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University)
  • 발행 : 2005.06.30
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초록

Pro-inflammatory cytokines, such as tumor necrosis factor $(TNF)-{\alpha}$, interleukin-12 (IL-12) and interleukin $(IL-1)-{\beta}$, play a key role in causing inflammatory diseases, which are rheumatoid arthritis, Crohn's disease and sepsis. Accumulating evidences suggest that low level laser irradiation (LLLI) may have an anti-inflammatory action. However, there are few data regarding down regulation of Th1 immune response by using the diod typed laser emitting device for human patients. As a fundamental step in order to address this issue, we investigated immunological impact of the low level laser irradiation (10 mw laser diode with a wavelength of 630 nm) on expression of pro-inflammatory cytokines in murine immunocytes (splenocytes and peritoneal macrophages) in vitro. The LLLI on lipopolysaccharide (LPS 100 ng/ml)-stimulated murine splenocytes and macrophages, clearly down regulated mRNA expression of $TNF-{\alpha}$ and IL-12 in dose-dependent manner. In addition, LLLI significantly inhibits the NO production in the LPS-stimulated murine macrophages. This data suggests that LLLI (wavelength of 630 nm) may exert an anti-inflammatory action via modulation of pro-inflammatory cytokine and NO production pathway.

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참고문헌

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