Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effects of Green Tea [Camellia sinensis (L.) O. Kuntze] Extract on Lipid Metabolism in F1B Golden Syrian Hamsters Fed with the Atherogenic Diet

녹차[Camellia sinensis (L.) O. Kuntze] 추출물의 투여가 동맥경화 유발식이를 급여한 F1B Golden Syrian hamster의 지질대사에 미치는 영향

  • Published : 2007.04.30
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Abstract

This study examined the effects of green tea extract supplementation (500 or 1,000 mg/kg b.w. per day) in conjunction with an atherogenic diet (10% coconut oil (w/w), 0.1% cholesterol) on plasma lipid composition, regression of pre-existing foam cells, and on the mRNA levels of hepatic HMG-CoA reductase and LDL receptor. Compared to groups fed only with the atherogenic diet, the addition of green tea extract to atherogenic diet-fed groups significantly down-regulated plasma triglyceride and total cholesterol levels, dose-dependently. Supplementation of 1,000 mg/kg b.w. of green tea extract with the atherogenic diet induced significant up-regulation of both HMG-CoA reductase and LDL receptor messenger RNA levels in liver as compared to the group receiving green tea extract supplementation at 500 mg/kg b.w. The F1B hamsters fed the atherogenic diet had greater foam cell accumulation compared to those fed a normal diet, or the atherogenic diet supplemented with green tea extract. Regression of fatty streak lesions was achieved by atherosclerosis in fat- and cholesterol-fed hamsters and this effect was associated with down-regulation of plasma cholesterol and up-regulation of hepatic LDL receptor expression.

8주령의 F1B golden Syrian 햄스터에게 동맥경화 유발식이와 함께 녹차 추출물을 각각 500 혹은 1,000 mg/kg b.w.의 양을 매일 경구투여하면서 6주간 사육하였을 때 햄스터 체내의 지질대사와 대동맥 내에서의 지방의 축적 정도에 미치는 영향을 조사하였다. 실험 결과 녹차추출물의 경구투여는 동맥경화유발식이를 섭취하는 햄스터의 혈중 중성지방과 총콜레스테롤치를 농도의존적으로 감소시켰고, 대동맥궁내에서의 지방의 축적을 예방하였다. 특히 녹차추출물 1,000 mg/kg b.w. 투여는 동맥경화유발식이를 섭취한 햄스터에서 간장내의 LDL receptor mRNA level을 증가시키는 경향을 보였다. 이러한 결과로 볼 때 녹차 추출물의 투여는 동맥경화유발식이를 섭취한 햄스터의 혈중 콜레스테롤치를 감소시키고 LDL receptor의 발현을 증가시킴으로써 동맥경화를 예방할 수 있음을 보여주었다.

Keywords

References

  1. KNSO. Annual Report on the Cause of Death Statistics. Korea National Statistical Office, Seoul, Korea (2003)
  2. Yoo EH. Hypercholesterolemia and Cardiovascular Disease. 2nd ed. Han Press, Seoul, Korea (2003)
  3. Davey SG, Pekkannen J. Should there be a moratorium on the use of cholesterol lowering drugs? Brit. Med. J. 304: 431-434 (1992) https://doi.org/10.1136/bmj.304.6824.431
  4. Ikeda K, Negishi H, Yamori Y. Antioxidant nutrients and hypoxia/ ischemia brain injury in rodents. Toxicology 189: 55-61 (2003) https://doi.org/10.1016/S0300-483X(03)00152-5
  5. Shepherd J, Cobbe SM, Ford I, Isles CG, Lorimer AR, McFarline PW, McKillop JH, Packard CJ. Prevention of coronary artery disease with prevention in men with hypercholesterolemia. West of Scotland Coronary Prevention Study Group. New Engl. J. Med. 333: 1301-1307 (1995) https://doi.org/10.1056/NEJM199511163332001
  6. Sacks FM, Moy LA, Davis BR, Cole TG, Rouleau JL, Nash DT, Pfeffer MA, Braunwald E. Relationship between plasma LDL concentrations during treatment with pravastatin and recurrent coronary events in the cholesterol and recurrent events trial. Circulation 97: 1446-1452 (1998) https://doi.org/10.1161/01.CIR.97.15.1446
  7. Marchand LL. Cancer preventive effects of flavonoids-a review. Biomed. Pharmacother. 56: 296-301 (2002) https://doi.org/10.1016/S0753-3322(02)00186-5
  8. Sabu MC, Smitha K. Ramadasan K. Anti-diabetic activity of green tea polyphenols and their role in reducing oxidative stress in experimental diabetes. J. Ethnopharmacol. 83: 109-116 (2002) https://doi.org/10.1016/S0378-8741(02)00217-9
  9. Dufresne CJ, Farnworthand ER. A review of latest research findings on the health promotion properties of tea. J. Nutr. Biochem. 12: 404-421 (2001) https://doi.org/10.1016/S0955-2863(01)00155-3
  10. Dorfman SE, Lichtenstein AH. Dietary fatty acids differentially modulate messenger RNA abundance of low-density lipoprotein receptor, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and microsomal triglyceride transfer protein in Golden-Syrian hamsters. Metab. Clin. Exp. 55: 635-641 (2006) https://doi.org/10.1016/j.metabol.2005.12.005
  11. Rein D, Yokoyama WH, Xu R, Walzem RL, German JB. Dietary vitamin E in an atherogenic hamster model. Nutr. Res. 18: 567-579 (1998) https://doi.org/10.1016/S0271-5317(98)00043-8
  12. Pitman WA, Osgood DP, Smith D, Schaefer EJ, Ordovas JM. The effect of diet and lovastatin on regression of fatty streak lesions and on hepatic and intestinal mRNA levels for the LDL receptor and HMG-CoA reductase in F1B hamsters. Atherosclerosis 138: 43-52 (1998) https://doi.org/10.1016/S0021-9150(97)00302-X
  13. Spady DK, Dietschy JM. Interaction of dietary cholesterol and triglyceride in the regulation of hepatic low density lipoprotein transport in the hamster. J. Clin. Invest. 81: 300-309 (1988) https://doi.org/10.1172/JCI113321
  14. Spady DK, Dietschy JM. Interaction of aging and dietary fat in the regulation of low density lipoprotein transport in the hamster. J. Lipid Res. 30: 559-569 (1989)
  15. Woollett LA, Spady DK, Dietschy JM. Mechanisms by which saturated triacylglycerols elevate the plasma low density lipoprotein- cholesterol concentration in hamsters: Differential effects of fatty acid chain length. J. Clin. Invest. 84: 119-128 (1989) https://doi.org/10.1172/JCI114131
  16. Kim YE, Oh SW, Kwon EK, Han D, Kim IH, Lee CH. Effects of green tea, buckwheat and grape leaves extracts in lipid metaboilism, antioxidant capacity and antithrombotic activity in rats fed high cholesterol diets. Korean J. Food Sci. Technol. 36: 979-985 (2004)
  17. Woollett LA, Spady DK, Dietschy JM. Saturated and unsaturated fatty acids independently regulate low density lipoprotein receptor activity and production rate. J. Lipid Res. 33: 77-88 (1992)
  18. Otto J, Ordovas JM, Smith D, Dongen DV, Nicolosi RJ, Schaefer EJ. Lovastatin inhibits diet induced atherosclerosis in F1B golden syrian hamsters. Atherosclerosis 114: 19-28 (1995) https://doi.org/10.1016/0021-9150(94)05457-T
  19. La Ville AE, Seddon AM, Shaikh M, Rowles PM, Woolf N, Lewin B. Primary prevention of atherosclerosis by lovastatin in a genetically hyperlipidemic rabbit strain. Atherosclerosis 78: 205-210 (1989) https://doi.org/10.1016/0021-9150(89)90224-4
  20. Kobayashi M, Ishida F, Takahashi T, Taguchi K, Watanabe K, Ohmura I, Kamei T. Preventive effect of MK-733 (simvastatin), an inhibitor of HMG-CoA reductase, on hypercholesterolemia and atherosclerosis induced by cholesterol feeding in rabbits. Jpn. J. Pharmacol. 49: 125-133 (1989) https://doi.org/10.1254/jjp.49.125
  21. Hadjiisky P, Hermier D, Truffert J, De Gennes JL, Grosgogeat Y. Effect of pravastatin, a HMG-CoA reductase inhibitor, on blood lipids and aortic lipidosis in cholesterol-fed White Carneau pigeons. Biochim. Biophys. Acta 1181: 279-286 (1993) https://doi.org/10.1016/0925-4439(93)90033-W
  22. Uehara Y, Urata H, Ideishi M, Arakawa K, Saku K. Chymase inhibition suppresses high-cholesterol diet-induced lipid accumulation in the hamster aorta. Cardiovasc. Res. 55: 870-876 (2002) https://doi.org/10.1016/S0008-6363(02)00458-3
  23. Nistor A, Bulla A, Filip DA, Radu A. The hyperlipidemic hamster as a model of experimental atherosclerosis. Atherosclerosis 68: 159-173 (1987) https://doi.org/10.1016/0021-9150(87)90106-7
  24. Vinson JA, Teufel K, Wu N. Green and black teas inhibit atherosclerosis by lipid, antioxidant, and fibrinolytic mechanisms. J. Agr. Food Chem. 52: 3661-3665 (2004) https://doi.org/10.1021/jf035255l
  25. Faia KL, Davis WP, Marone AJ, Foxall TL. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in hamster aortic atherosclerosis: correlation with in-situ zymography. Atherosclerosis 160: 325-337 (2002) https://doi.org/10.1016/S0021-9150(01)00590-1
  26. Chambers CM, Ness GC. Dietary cholesterol regulates hepatic 3-hydroxy- 3-methylglutaryl coenzyme A reductase gene expression in rats primarily at the level of translation. Arch. Biochem. Biophys. 354: 317-322 (1998) https://doi.org/10.1006/abbi.1998.0689
  27. Bursill CA, Abbey M, Roach PD. A green tea extract lowers plasma cholesterol by inhibiting cholesterol synthesis and upregulating the LDL receptor in the cholesterol-fed-rabbit. Atherosclerosis: In press. (2006)
  28. Harwood HJ, Chandler CE, Pellearin LD. Pharmacologic consequences of cholesterol absorption inhibition: alteration in cholesterol metabolism and reduction in plasma cholesterol concentration induced by the synthetic saponin ${\beta}$-tigogenin cellobioside (CP-88818; tiqueside). J. Lipid Res. 34: 377-395 (1993)
  29. Brown MS, Goldstein JL, A receptor-mediated pathway for cholesterol homeostasis. Science 232: 34-47 (1986) https://doi.org/10.1126/science.3513311
  30. Chan PT, Fong WP, Cheung YL. Jasmine green tea epicatechins are hypolipidemic in hamsters (Mesocricetus auratus) fed a high fat diet. J. Nutr 129: 1094-1101 (1999)
  31. Yang TT, Koo MW. Chinese green tea lowers cholesterol level through an increase in fecal lipid excretion. Life Sci. 66: 411-423 (2000)
  32. Kovanen PT, Bilheimer DW, Goldstein JL, Jaramillo JJ, Brown MS, Regulatory role for hepatic low density lipoprotein receptors in vivo in the dog. P. Natl. Acad. Sci. USA 78: 1194-1198 (1981) https://doi.org/10.1073/pnas.78.2.1194
  33. Kita T, Brown MS, Goldstein JL, Feedback regulation of 3-hydroxy- 3-methylglutaryl coenzyme A reductase in livers of mice treated with mevinolin, a competitive inhibitor of the reductase. J. Clin. Invest. 66: 1094-1100 (1980) https://doi.org/10.1172/JCI109938
  34. Adams CWM, Bayliss OB. Lipid Histochemistry. Vol. 2, pp. 99-156. In: Techniques of Biochemical and Biophysical Morphology. Glick D (ed). Wiley, New York, NY, USA (2002)
  35. Kowala MC, Nunnari JI, Durham SK, Nicolosi RJ. Doxazosin and cholestyramine similarly decrease fatty streak formation in the aortic arch of hyperlipidemic hamsters. Atherosclerosis 91: 35-49 (1991) https://doi.org/10.1016/0021-9150(91)90185-6
  36. Foxall TL, Shwaery GT, Stucchi AF, Nicolosi RJ, Wong SS. Dose-related effects of doxazosin on plasma lipids and aortic fatty streak formation in the hyperlipidemic hamster model. Am. J. Pathol. 140: 1357-1363 (1992)
  37. Liu CH, Huang MT, Huang PC. Sources of triglycerol accwnulation in liver of rats fed a cholesterol supplemented diets. Lipids 30: 527-531 (1995) https://doi.org/10.1007/BF02537027
  38. Brown DW, Giles WH, Croft JB. Left ventricular hypertrophy as a predictor of coronary heart disease mortality and the effect of hypertension. Am. Heart J. 140: 848-856 (2000) https://doi.org/10.1067/mhj.2000.111112