• Steel and Composite Structures
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• v.10 no.1
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• pp.23-43
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• 2010

In this paper, a nonlinear model is developed using the component method in order to represent the response of steel connections under various loading conditions and temperature variations. The model is capable of depicting the behaviour of a number of typical connection types including endplate forms (extended and flush) and angle configurations (double web, top and seat, and combined top-seat-web) in both steel and composite framed structures. The implementation is undertaken within the finite element program ADAPTIC, which accounts for material and geometric nonlinearities. Verification of the proposed connection model is carried out by comparing analytical simulations with available results of isolated joint tests for the ambient case, and isolated joint as well as sub-frame tests for elevated temperature conditions. The findings illustrate the reliability and efficiency of the proposed model in capturing the stiffness and strength properties of connections, hence highlighting the adequacy of the component approach in simulating the overall joint behaviour at elevated temperature.

• Journal of Microbiology and Biotechnology
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• v.17 no.3
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• pp.539-542
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• 2007

Glycosyltransferase family 1 (UOT) uses small chemicals including phenolics, antibiotics, and alkaloids as substrates to have an influence in biological activities. A glycosyltransferase (XcGT-2) from Xanthomonas campestris was cloned and consisted of a 1,257 bp open reading frame encoding a 45.5 kDa protein. In order to use this for the modification of phenolic compounds, XcGT-2 was expressed in Escherichia coli as a glutathione S-transferase fusion protein. With the E. coli transformant expressing XcGT-2, biotransformation of flavonoids was carried out. Flavonoids having a double bond between carbons 2 and 3, and hydroxyl groups at both C-3' and C-4', were glycosylated and the glycosylation position was determined to be at the hydroxyl group of C-3', using nuclear magnetic resonance spectroscopy. These results showed that XcGT-2 regiospecifically transferred a glucose molecule to the 3'-hydroxyl group of flavonoids containing both 3' and 4'-hydroxyl groups.

• Steel and Composite Structures
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• v.18 no.6
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• pp.1405-1421
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• 2015

Semi-rigid connections are the actual behavior of beam-to-column connections in steel frames. However, the behavior of semi-rigid connections is not taken into account for the simplicity in the conventional analysis and design of steel frames. A computer-based analysis and design has been studied for the three-dimensional steel frames with semi-rigid connections. The nonlinear analysis which includes the effects of the flexibility of connections is used for this study. It is designed according to the buckling and combined stress constraints under the present loading after the joint deformations and the member end forces of the space frame are determined by the stiffness matrix method. The semi-rigid connection type is limited to the top and bottom angles with a double web angle connection. The Frye-Morris polynomial model is used to describe the non-linear behavior of semi-rigid connections. Various design examples are presented to demonstrate the efficiency of the method. The results of design and analysis of unbraced semi-rigid frames are compared to the results of unbraced rigid frames under the same design requirements.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.2
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• pp.44.2-44.2
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• 2017

We obtained near-infrared spectra of 26 SDSS quasars at 0.7${\sim}10^{{\wedge}{10}}M{\odot}$ to critically examine the systematic effects involved with their mass estimations. We find that active galactic nuclei (AGNs) heavier than $10^{{\wedge}{10}}M{\odot}$ often display double-peaked $H{\alpha}$ emission, extremely broad FeII complex emission around MgII, and highly blueshifted and broadened CIV emission. The weight of this evidence, combined with previous studies, cautions against the use of MBH values based on any emission line with a width over 8000 km/s. Also, the MBH estimations are not positively biased along the presence of ionized narrow line outflows, anisotropic radiation, or the use of line FWHM instead of ${\sigma}$ for our sample, and unbiased with variability, scatter in broad line equivalent width, or obscuration for general type-1 quasars. Removing the systematically uncertain MBH values, ${\sim}10^{{\wedge}{10}}M{\odot}$ BHs in 1${\sim}10^{{\wedge}{9.5}}M{\odot}$ BHs, although current observations support they are intrinsically most massive, and overmassive to the host's bulge mass.

• ALGAE
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• v.18 no.3
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• pp.191-197
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• 2003

Histones are important proteins that interact with the DNA double helix to form nucleosome. Two putative histone genes, GjH2A-1 and GjH2A-2 were isolated from a red alga Griffithsia japonica. The putative open reading frame of GjH2A-1 and GjH2A-2 shared high similarity with the previously reported amino acid sequences of histone H2As. They have a motif consisting of seven amino acids A-G-L-Q-F-P-V, which matches the histone H2A motif [AC]-G-L-x-F-P-V. Phylogenetic trees were constructed from amino acid sequences of 38 histone H2As. The histone H2As were divided into two groups: major H2As and H2A.F/Z variants. The major histone H2A group consisted of animals, fungi, plants + green algae, and red algae H2A subgroups. The animal histone H2A subgroup was divided into vertebrates, echinoderms, nematodes, insects, and segmented worms H2As. The putative red algal histone genes, GjH2A-1 and GjH2A-2, constituted an independent lineage. This is the first report on red algal histone genes.

• Journal of Ocean Engineering and Technology
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• v.23 no.1
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• pp.16-23
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• 2009

The three-dimensional ship motion with forward speed was solved by a finite element method in the time domain. A boundary value problem was described in the frame of a fixed-body reference, and the problem was formulated according to Double-Body and Neumann-Kelvin linearizations. Laplace's equation with boundary conditions was solved by a classical finite element method based on the weak formulation. Chebyshev filtering was used to get rid of an unwanted saw-tooth wave and a wave damping zone was adopted to impose a numerical radiation condition. The time marching of the free surface was performed by the 4th order Adams-Bashforth-Moulton method. Wigley I and Wigely III models were considered for numerical validation. The hydrodynamic coefficients and wave exciting forces were validated by a comparison with experimental data and the numerical results of the Wigley I. The effects of the linearization are also discussed. The motion RAO was also checked with a Wigley III model through mono-chromatic and multi-chromatic regular waves.

• Steel and Composite Structures
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• v.26 no.5
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• pp.573-582
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• 2018

This study develops and numerically verifies an innovative seismically resilient bracing system. The proposed self-centering tension-only brace (SC-TOB) is composed of a tensioning system to provide a self-centering response, a frictional device for energy dissipation, and a high-strength steel cable as a bracing element. It is considered to be an improvement over the traditional self-centering braces in terms of lightness, high bearing capacity, load relief, and double-elongation capacity. In this paper, the mechanics of the system are first described. Governing equations deduced from the developed analytical model to predict the behavior of the system are then provided. The results from a finite element validation confirm that the SC-TOB performs as analytically predicted. Key parameters including the activation displacement and load, the self-centering parameter, and equivalent viscous damping are investigated, and their influences on the system behavior are discussed. Finally, a design procedure considering controlled softening behavior is developed and illustrated through a design example.

• Fisheries and Aquatic Sciences
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• v.10 no.2
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• pp.60-67
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• 2007

Myostatin (MSTN; also known as GDF8) is a member of the transforming growth factor ${\beta}-superfamily$ of proteins. MSTN negatively regulates mammalian skeletal muscle growth and development by inhibiting myoblast proliferation. Mice and cattle possessing mutant MSTN alleles display a 'double muscling' phenotype characterized by extreme skeletal muscle hypertrophy and/or hyperplasia. We isolated the full-length cDNA of a novel MSTN gene from S. schlegeli muscle tissue and examined its expression pattern in various tissues. The full-length gene (GenBank DQ423474) consists of 1941bp with an open reading frame of 1134 bp, encoding 377 amino acids that show 62-92% amino acid similarity to other vertebrate MSTNs. The predicted protein contains a conserved proteolytic cleavage site (RXRR) and nine conserved cysteine residues at the C terminus. RT-PCR revealed that the unprocessed and prodomain myostatin mRNAs were predominantly present in muscle, with limited expression in other tissues. However, the mature myostatin mRNA was highly expressed in brain and muscle, intermediately expressed in the gills, intestine, heart, and kidney, and weakly expressed in the liver and spleen.

• Architectural research
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• v.8 no.2
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• pp.17-26
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• 2006

This paper attempts to identify the term "tectonics" comprehensively by collecting and categorizing existing definitions of tectonics within the architectural area rather than to stress the concept of tectonics of each specific theorist. Although no consensus of opinion on the concept of tectonics exists, architectural tectonics was closely related to the following terms in three categories: 1. $techn\acute{e}$, technique, and technology; 2. construction and structure; and 3. stereotomics. Based on its etymology, system, and material construct, the notion of tectonics common in these three categories signifies "the art of framing construction," in which linear elements are connected with joints and clad or infilled with lightweight material. Thus, the art of framing construction, as a common concept of tectonics, reveals the following characteristics: First, tectonics is based on framing construction in contrast to piling-up construction as the etymology of tectonics signifies the art of carpentry. Then, the term tectonics, dealing as it does with a higher level of construction rather than the mechanical level of structure, incorporates the poetic aspect of techne as well as the rational aspect of technology. Third, Owing to the organic, double system of tectonic frame and incrusting or infilling materials, the tectonic body becomes both the ornament and the structure simultaneously. As the art of framing construction is based on material construction rather than structural or ornamental form, this paper proposes that one can view tectonics as a term that conveys the meaning of the actual material effect on space.

• The korean journal of orthodontics
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• v.16 no.2
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• pp.43-51
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• 1986

The highly accurate laser holographic interferemotry method was used to determine in what way low-magnitude forces during maxillary expansion are transmitted to the entire maxillary complex and its surrounding structures. The experiments were carried out on a dryed human skull which had a perfectly preserved, normally aligned maxillary dental arch and intact alveolar process. The skull was fixed within a constructed metal frame which ensured maximal stability of the object. The optical equipment and the object were mounted on antivibration table. Interferograms were taken on the lateral and frontal sides of the maxillary complex, using the 10mW He-Ne laser and the double-exposure method. Analysis of the fringe pattern on the recorded object surface was performed by graphically determining the deformation curves related to the bony surface in selected horizontal and vertical planes. On the basis of this study, the following conclusions can be drawn: 1. The density of the interference fringes was gradually increased with the degree of expansion force. 2. Mechanical reactions on the maxillary complex, circummaxillary sutures, and surrounding bones were clearly visible, even with the lowest loading degree. 3. The amount of bone displacement was greater in application of the force after $90^{\circ}$ turn than in initial application of the same force. 4. The direction of interference fringes on the bony surface was similar at all loading degrees.