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Glycosylation of Flavonoids with E. coli Expressing Glycosyltransferase from Xanthomonas campestris  

Kim, Jeong-Ho (Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University)
Kim, Bong-Gyu (Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University)
Kim, Jae-Ah (Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University)
Park, Young-Hee (Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University)
Lee, Yoon-Jung (Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University)
Lim, Yoong-Ho (Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University)
Ahn, Joong-Hoon (Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.3, 2007 , pp. 539-542 More about this Journal
Abstract
Glycosyltransferase family 1 (UOT) uses small chemicals including phenolics, antibiotics, and alkaloids as substrates to have an influence in biological activities. A glycosyltransferase (XcGT-2) from Xanthomonas campestris was cloned and consisted of a 1,257 bp open reading frame encoding a 45.5 kDa protein. In order to use this for the modification of phenolic compounds, XcGT-2 was expressed in Escherichia coli as a glutathione S-transferase fusion protein. With the E. coli transformant expressing XcGT-2, biotransformation of flavonoids was carried out. Flavonoids having a double bond between carbons 2 and 3, and hydroxyl groups at both C-3' and C-4', were glycosylated and the glycosylation position was determined to be at the hydroxyl group of C-3', using nuclear magnetic resonance spectroscopy. These results showed that XcGT-2 regiospecifically transferred a glucose molecule to the 3'-hydroxyl group of flavonoids containing both 3' and 4'-hydroxyl groups.
Keywords
Biotransformation; flavonoids; glycosyltransferase;
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Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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