• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1635-1643
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• 2009

The aim of this study was to provide new insight into the mechanism whereby the housekeeping enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) locates to cell walls of Lactobacillus plantarum 299v. After purification, cytosolic and cell wall GAPDH (cw-GAPDH) forms were characterized and shown to be identical homotetrameric active enzymes. GAPDH concentration on cell walls was growth-time dependent. Free GAPDH was not observed on the culture supernatant at any time during growth, and provoked cell lysis was not concomitant with any reassociation of GAPDH onto the cell surface. Hence, with the possibility of cw-GAPDH resulting from autolysis being unlikely, entrapment of intracellular GAPDH on the cell wall after a passive efflux through altered plasma membrane was investigated. Flow cytometry was used to assess L. plantarum 299v membrane permeabilization after labeling with propidium iodide (PI). By combining PI uptake and cw-GAPDH activity measurements, we demonstrate here that the increase in cw-GAPDH concentration from the early exponential phase to the late stationary phase is closely related to an increase in plasma membrane permeability during growth. Moreover, we observed that increases in both plasma membrane permeability and cw-GAPDH activity were delayed when glucose was added during L. plantarum 299v growth. Using a double labeling of L. plantarum 299v cells with anti-GAPDH antibodies and propidium iodide, we established unambiguously that cells with impaired membrane manifest five times more cw-GAPDH than unaltered cells. Our results show that plasma membrane permeability appears to be closely related to the efflux of GAPDH on the bacterial cell surface, offering new insight into the understanding of the cell wall location of this enzyme.

• Journal of KIISE:Software and Applications
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• v.30 no.3_4
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• pp.251-258
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• 2003

A support vector machine (SVM) is supposed to provide a good generalization performance, but the actual performance of a actually implemented SVM is often far from the theoretically expected level. This is largely because the implementation is based on an approximated algorithm, due to the high complexity of time and space. To improve this limitation, we propose ensemble of SVMs by using Bagging (bootstrap aggregating) and Boosting. By a Bagging stage each individual SVM is trained independently using randomly chosen training samples via a bootstrap technique. By a Boosting stage an individual SVM is trained by choosing training samples according to their probability distribution. The probability distribution is updated by the error of independent classifiers, and the process is iterated. After the training stage, they are aggregated to make a collective decision in several ways, such ai majority voting, the LSE(least squares estimation) -based weighting, and double layer hierarchical combining. The simulation results for IRIS data classification, the hand-written digit recognition and Face detection show that the proposed SVM ensembles greatly outperforms a single SVM in terms of classification accuracy.

• Journal of Family Resource Management and Policy Review
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• v.15 no.3
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• pp.131-151
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• 2011

This study attempted to estimate the level of perceived lack of time in Korean society, to link it to time management and to identify the possible association that accounts for time shortage. I employed the gender perspective in order to reveal the dynamics and complexity of the perception of a lack of time in the population. The sample for the study was drawn from the 1999 and 2009 Korean time diary data collected by the Korean National Statistical Office. From the original data, I selected a sample of second shift families(men and women who are presently working and raising children) living in metropolitan areas(including Seoul and six major urban areas in Korea). The dependent variable was time pressure measured by a single-item question on a four-point likert scale. The results of the study showed that working mothers perceived a greater time shortage as compared to working fathers. The time use pattern showed change during the periods, indicating that people worked fewer hours in paid labor and enjoyed more leisure hours and personal care hours. However, on average, people seemed to have experienced a greater time lack in 2009 as compared to 1999. The results from the ordered logistic regression model revealed that even though there were similarities in the impact of relevant factors, men's perception of a lack of time was more closely linked with their work role and social status, while for women, this perception was influenced by work and family duties. This indicates that Korean working mothers and fathers are facing a double jeopardy of time shortage in terms of combining their work and family roles. As a result, the level of time pressure by gender is converging toward a "never enough" phase. These findings generated policy implications and detailed suggestions.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.5D
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• pp.671-677
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• 2011

A diversity of railway network function enhancement projects such as the double tracking, electrification, and direct operation have been actively executed to improve the railway service. When the new rapid transit is provided, how many people will use it instead of other transports? How will the railway choice behavior be changed? Accordingly, in this paper, the applicability of diverted travel demand forecast methods, by Revealed Preference(RP) and Stated Preference(SP) data was reviewed for Daegu metropolitan rail rapid transit service. As the result of combining RP and SP data, including the sequential and simultaneous approach, the total travel time and travel cost parameters are of the right sign and are highly significant. The simultaneous approach is more efficient in terms of the estimation of coefficients. In particular, methods to improve validity of the Mixed RP/SP models, when RP data is used proportionally, the diverted travel demand can be easily identified by railway fare and travel time service level. Therefore, it is considered that this will practically apply even in other regions as well as Daegu metropolitan railway.

• The Journal of the Korea Contents Association
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• v.20 no.3
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• pp.190-199
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• 2020

Globally, more super-tall buildings tend to be constructed competitively in the social and economic foundations. In the circumstance, this study is aimed at establishing a paradigm of super-tall buildings in terms of their various forms. Symbolizing a city or state, super-tall buildings not only are used as resources of tourism, but play an important role as a characteristic landmark. Therefore, it is necessary to find a curved form for a futuristic perspective. The purpose of this study is to infer the convergence relativity of curved forms among complex and diverse unstructured construction forms. This study used as subjects 50 super-tall buildings among the ranking data selected Council on Tall Buildings and Urban Habitat (CTBUH) in order for the basis of constructability related to actual design, rather than the way of recognizing a formative type, in the classification of curved forms into regularized surfaces, developable surfaces, and double-curved surfaces. The results of this study are presented as follows. This classification can be used as a fundamental material which is reasonably involved in the design process pursuing diverse curved surfaces in terms of design of tall buildings.

• Journal of Electrical Engineering and Technology
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• v.5 no.2
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• pp.337-341
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• 2010

In this paper, an extremely low voltage operated micro corner cube retroreflector (CCR) was fabricated for free-space optical communication applications by using bulk silicon micromachining technologies. The CCR was comprised of an orthogonal vertical mirror and a horizontal actuated mirror. For low voltage operation, the horizontal actuated mirror was designed with two PZT cantilever actuators, torsional bars, hinges, and a mirror plate with a size of $400{\mu}m{\times}400{\mu}m$. In particular, the torsional bars and hinges were carefully simulated and designed to secure the flatness of the mirror plate by using a finite element method (FEM) simulator. The measured tilting angle was approximately $2^{\circ}$ at the applied voltage of 5 V. An orthogonal vertical mirror with an extremely smooth surface texture was fabricated using KOH wet etching and a double-SOI (silicon-on-insulator) wafer with a (110) silicon wafer. The fabricated orthogonal vertical mirror was comprised of four pairs of two mutually orthogonal flat mirrors with $400{\mu}m4 (length) $\times400{\mu}m$ (height) $\times30{\mu}m$ (thickness). The cross angles and surface roughness of the orthogonal vertical mirror were orthogonal, almost $90^{\circ}$ and 3.523 nm rms, respectively. The proposed CCR was completed by combining the orthogonal vertical and horizontal actuated mirrors. Data transmission and modulation at a frequency of 10 Hz was successfully demonstrated using the fabricated CCR at a distance of approximately 50 cm.

• Korean Journal of Chemical Engineering
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• v.35 no.12
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• pp.2336-2347
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• 2018

The azeotrope of methyl acetate methanol and water was isolated using extractive distillation with water as entrainer. The pressure-swing extractive distillation (PSED) process and vapor side-stream distillation column (VSDC) with the rectifier process were designed to separate the methyl acetate, methanol and water mixture. It was revealed that the VSDC with the rectifier process had a reduction in energy consumption than the PSED process. Four control schemes of the two process were investigated: Double temperature control scheme (CS1), $Q_R/F$ feedforward control of reboiler duty scheme for PESD (CS2), $Q_R/F$ feedback control scheme for VSDC (CS3), the feedback control scheme of sensitive plate temperature of side-drawing distillation column to dominate the compressor shaft speed (CS4). Feed flow and composition disturbance were used to evaluate the dynamic performance. As a result, CS4 is a preferable choice for separation of methyl acetate-methanol-water mixture. A control scheme combining the operating parameters of dynamic equipment with the control indicators of static equipment was proposed in this paper. It means using the sensitive plate temperature of side-drawing column to control the compressor shaft speed. This is a new control scheme for extractive distillation.

• Journal of The Korean Society of Clinical Toxicology
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• v.16 no.2
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• pp.149-156
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• 2018

Purpose: The purpose of this study was to determine whether hepatotoxicity could be predicted early using biochemical markers in patients with acetaminophen (AAP) poisoning and to assess the usefulness of predictive factors for acute liver injury or hepatotoxicity. Methods: This study was a retrospective observational study involving a medical records review. The participants were patients who were admitted to the emergency department (ED) with AAP overdose at two hospitals over a 10-year period. Demographic data, age, time from ingestion to visit, initial AAP level, initial hepatic aminotransferases, and initial prothrombin time were recorded. Acute liver injury was defined as a peak serum ALT >50 U/L or double the admission value, and hepatotoxicity was defined as a peak ALT >1,000 U/L. Receiver operating characteristic curve analyses were performed to compare the prognostic performance among variables. Results: A total of 97 patients were admitted to the ED with AAP overdose, of whom 26 had acute liver injury and 6 had hepatotoxicity. Acute liver injury was associated with the time interval after taking the drug, and hepatotoxicity was associated with the initial PT and the ALT level. The scoring system proposed by the authors has a significant ability to predict both acute liver injury and hepatotoxicity. Conclusion: To predict the prognosis of AAP poisoning patients, the time interval after taking AAP was important, and initial prothrombin time and ALT level were useful tests. Also a scoring system combining variables may be useful.

• The Journal of the Convergence on Culture Technology
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• v.9 no.3
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• pp.347-358
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• 2023

This study attempted a convergence approach of family therapy and family coaching as the importance of convergence application of counseling and coaching has rapidly spread around the academic and clinical world with one individual client around the world since the 2000s. For this, based on previous domestic and foreign literature studies, coaching techniques that can increase clinical satisfaction and effectiveness by combining with coaching among the main theories of family therapy were presented. This researcher examined whether family therapy can be applied convergently with family coaching, and what benefits are available when the main theories of family therapy are combined with coaching in the clinical field. For this purpose, double binding and communication coaching, pseudo-mutuality and true rapport, marital schism and conflict mediation, differentiation and dissociation were compared and analyzed. Based on this study, it is hoped that a convergence approach of family therapy and family coaching will be attempted in the field of counseling and psychotherapy, and through this, it will contribute to establishing a new clinical treatment model and academic establishment of family coaching.

• Journal of Chest Surgery
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• v.32 no.7
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• pp.621-627
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• 1999

Background: Sixty five cases with congenitally corrected transposition of the great arteries (CCTGA) indicated for biventricular repair were operated on between 1984 and september 1998. Comparison between the results of the conventional(classic) connection(LV-PA) and the anatomic repair was done. Material and Method: Retrospective review was carried out based on the medical records of the patients. Operative procedures, complications and the long-term results accoding to the combining anomalies were analysed. Result: Mean age was 5.5$\pm$4.8 years(range, 2 months to 18years). Thirty nine were male and 26 were female. Situs solitus {S,L,L} was in 53 and situs inversus{I,D,D} in 12. There was no left ventricular outflow tract obstruction(LVOTO) in 13(20%) cases. The LVOTO was resulted from pulmonary stenosis(PS) in 26(40%)patients and from pulmonary atresia(PA) in 26(40%) patients. Twenty-five(38.5%) patients had tricuspid valve regurgitation(TR) greater than the mild degree that was present preoperatively. Twenty two patients previously underwent 24 systemic- pulmonary shunts previously. In the 13 patients without LVOTO, 7 simple closure of VSD or ASD, 3 tricuspid valve replacements(TVR), and 3 anatomic corrections(3 double switch operations: 1 Senning+ Rastelli, 1 Senning+REV-type, and 1 Senning+Arterial switch opera tion) were performed. As to the 26 patients with CCTGA+VSD or ASD+LVOTO(PS), 24 classic repairs and 2 double switch operations(1 Senning+Rastelli, 1 Mustard+REV-type) were done. In the 26 cases with CCTGA+VSD+LVOTO(PA), 19 classic repairs(18 Rastelli, 1 REV-type), and 7 double switch operations(7 Senning+Rastelli) were done. The degree of tricuspid regurgitation increased during the follow-up periods from 1.3$\pm$1.4 to 2.2$\pm$1.0 in the classic repair group(p<0.05), but not in the double switch group. Two patients had complete AV block preoperatively, and additional 7(10.8%) had newly developed complete AV block after the operation. Other complications were recurrent LVOTO(10), thromboembolism(4), persistent chest tube drainage over 2 weeks(4), chylothorax(3), bleeding(3), acute renal failure(2), and mediastinitis(2). Mean follow-up was 54$\pm$49 months(0-177 months). Thirteen patients died after the operation(operative mortality rate: 20.0%(13/65)), and there were 3 additional deaths during the follow up period(overall mortality: 24.6%(16/65)). The operative mortality in patients underwent anatomic repair was 33.3%(4/12). The actuarial survival rates at 1, 5, and 10 years were 75.0$\pm$5.6%, 75.0$\pm$5.6%, and 69.2$\pm$7.6%. Common causes of death were low cardiac output syndrome(8) and heart failure from TR(5). Conclusion: Although our study could not demonstrate the superiority of each classic or anatomic repair, we found that the anatomic repair has a merit of preventing the deterioration of tricuspid valve regurgitations. Meticulous selection of the patients and longer follow-up terms are mandatory to establish the selective advantages of both strategies.