• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2004년도 춘계학술대회 학술발표 논문집 제14권 제1호
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• pp.97-100
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• 2004

The identification of the DNA structure as a double-stranded helix consting of two nucleotide chain molecules was a milestone in modern molecular biology. The DNA chip technology is based on reverse hybridization that follows the principle of complementary binding of double-stranded DNA. DNA chip can be described as the deposition of defined nucleic acid sequences, probes, on a solid substrate to form a regular array of elements that are available for hybridization to complementary nucleic acids, targets. DNA chips based on cDNA clons, oligonucleotides and genomic clons have been developed for gene expression studies, genetic variation analysis and genomic changes associated with disease including cancers and genetic diseases. DNA chips for gene expression profiling can be used for functional analysis in human eel Is and animal models, disease-related gene studies, assessment of gene therapy, assessment of genetically modified food, and research for drug discovery. DNA chips for genetic variation detection can be used for the detection of mutations or chromosomal abnormalities in cnacers, drug resistances in cancer cells or pathogenic microbes, histocompatibility analysis for transplantation, individual identification for forensic medicine, and detection and discrimination of pathogenic microbes. The DNA chip will be generalized as a useful tool in clinical diagnostics in near future. Lab-on-a chip and informatics will facilitate the development of a variety of DNA chips for diagnostic purpose.

• 한국자기공명학회논문지
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• 제10권2호
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• pp.175-187
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• 2006

Binding interactions between a positively charged porphyrin derivative TMAP(meso-tetra(p-trimethylanilinium-4-yl)porphyrin) and triple helical $(dT)_{12}{\cdot}(dA)_{12}{\cdot}(dT)_{12}$, as well as double helical $(dA)_{12}{\cdot}(dT)_{12}$ have been studied with NMR, UV and CD spectroscopy to obtain the detailed information about the binding mode and binding site. UV melting studies showed both DNA duplex and triple helix represented very similar UV absorption patterns upon binding TMAP, but the presence of third strand of triple helical $(dT)_{12}{\cdot}(dA)_{12}{\cdot}(dT)_{12}$, inhibited improvement in thermal stability in terms of melting temperature, $T_m$. In addition, the TMAP molecule is thought to bind to the major groove, according to CD and NMR data. But absence of the clear isosbestic point in UV absorption spectra represented that binding of TMAP to DNA duplex as well as DNA triplex did not show a single binding mode, rather complex binding modes.

• 한국고분자학회:학술대회논문집
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• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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• pp.253-253
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• 2006

DNA has not been played the role as a biocatalyst in evolutionary history, although RNA and protein function as a biocatalyst. DNA double helix structure is believed to be impossible to form intricate active enzymatic sites. In addition, the chemical stability of DNA prevents the ability from self-modifying reactions. However, recent development of DNA engineering enables to create artificial enzymatic ability of DNA (deoxyribozyme) such as RNA cleavage and DNA modification. We investigated optimal conditions for enzymatic activity of DNA-Pt complex, and compared it with that of horse radish peroxidase. We report here that base sequence of DNA, pH and temperature affect the enzymatic activity of DNA-Pt complex.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2001년도 춘계학술대회 논문집(한국공작기계학회)
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• pp.28-33
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• 2001

Screw conveyors are used extensively in industrial for conveying and elevating materials. Despite their apparent simplicity, the mechanics of the conveying action is very complex. so many engineers depend on experiential data. Capacities of screw are pumping, steady flow of polymer melts, steady volumetric throughput etc. they are affected by geometry of screw, heat flux, pressure on inside barrel, rotating velocity, friction coefficient at screw surface etc. by computation volumetric efficiency increases as rotating velocity increases and decreases as friction coefficient increases. also it decreases with short pitch length. and double flight screw is more effective than single flight screw. The temperature of polymer melts by heating pad and injection pressure play a very important role in the injection molding machine. so in this paper we analyze thermal distortion and stress of screw includes pressure and temperature distributions by finite element analysis to understand what design factors influence on volumetric throughput efficiency of the screw and thermo-mechanical characteristics of screw.

• 약학회지
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• 제54권1호
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• pp.62-66
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• 2010

Benzopyrene is known to be one of the most powerful carcinogens which can build intercalated motif between base pairs in damaged DNA. The dimension of benzopyrene itself is much bigger than any of the DNA bases and thus the question whether the lesion of some base pair by insertion of benzopyrene can happen with or without a dramatic distortion of the helical structure is a highly interesting theme. In this work we used a molecular mechanics simulation using AMBER program package to go into the conformational characteristics. The condition of the insertion process of the benzopyrene motif from minor groove of the starting structure between the base pairs in the internal area of double helix was investigated using the molecular dynamics simulation at elevated temperature.

• 약학회지
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• 제54권1호
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• pp.67-74
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• 2010

Some of the benzopyrene (BP)-DNA adduct are known to build intercalated motif between flanking base pairs in damaged DNA depending on the structural condition. The size of benzopyrene itself is definitely not comparable with any of the DNA bases and thus the question whether the lesion of some base pair by insertion of benzopyrene can happen with or without a dramatic distortion of the helical structure is a highly interesting theme. In this work we used a molecular dynamics simulation based on the theory of molecular mechanics. The specific consequences about the structural properties of the intercalated structures and benzopyrene motif in minor groove of the double helix are deduced after 5 ns simulation time.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2005년도 한국컴퓨터종합학술대회 논문집 Vol.32 No.1 (B)
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• pp.262-264
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• 2005

MicroRNA는 약 22 nucleotide 길이로, 세포질에서 유전자의 전사 후 조절 기능을 맡는 small RNA의 한 종류이다. MiRNA는 긴 전사체인 pri-miRNA에서 Drosha에 의해 절단 되어 핵 밖으로 나가 최종 Dicer에 의해 성숙된다. 하지만, 아직까지 이 효소들이 pri-miRNA를 잘라내는 3차 구조상의 메커니즘을 이해하지 못하고 있다. 본 연구에서는 완숙한 miRNA 이중나선이 약 2 회전을 이루게 된다는 정보를 바탕으로, Drosha가 붙는 miRNA stem구조의 dinucleotide step 파라미터를 유전 알고리즘을 이용하여 추정한다. 추정된 파라미터로부터 실제 miRNA들의 3차구조를 예측할 수 있으며, 3차 구조상의 Drosha의 절단 메커니즘을 이해하는데 도움을 줄 것이다.

• 대한수학교육학회지:수학교육학연구
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• 제25권4호
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• pp.717-754
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• 2015

본 연구는 개별화된 경쟁에 치우쳐 있는 우리나라 수학교육 환경에서 고등학교 영재학생들에게 수학 발전의 사회적 과정을 경험할 수 있는 기회를 제공하기 위하여 온라인 탐구 커뮤니티의 건설을 시도하였다. 2012년 B과학고등학교에서 개설된 두 개의 미적분학 II 강좌를 수강하였던 14명의 학생들이 지정된 위키 사이트에 접속하여 약 70일간 10개의 문제를 풀었다. 협력 문제해결 과정에서 위키는 학생들의 흩어져 있는 사고과정을 공유되는 세계 내에 효과적으로 매개함으로써 상호학습이 이루어지는 것을 가능하게 하였다. 또한 학생들의 협력 문제해결의 패턴은 Lakatos(1976)의 '증명과 반박'과 비슷하게 '풀이와 반박'으로 특징지어졌으며 학생들은 이 과정을 통해 학교수학적 지식의 성장을 경험할 수 있었다. 실험 종료 후 실시된 인터뷰와 설문조사에서 담당교사와 학생들은 협력 문제해결 도구로서의 위키에 대해 매우 긍정적인 반응을 보였다. 따라서 본 연구에서 고등학교 영재학생들에게 위키는 수학적 지식의 사회적 측면에 대한 학습기회를 제공할 수 있는 가치 있는 수학교육 도구라고 평가된다.

• 한국유통학회지:유통연구
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• 제15권5호
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• pp.127-153
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• 2010

본 연구는 지난 10년간 급격하게 변화하여 온 온라인 쇼핑산업에 대해 성장의 동력을 이론적으로 확인하고, 이들에 의한 이론적 설명을 시도한 탐색적 연구이다. 이러한 과정에서 이론적으로 선점이론에 대한 검토를 통해 이 이론이 유통구조에서 온라인 유통산업에서 발생하고 있는 재중간상화와 배치됨을 확인하였고, 이에 따라서 동적 역량이론을 활용한 온라인 산업에 대한 이론적 적용모델인 NEBIC에 대한 검토를 통해 동적성장이론인 이중나선구조 모형을 제안하였다. 또한 이론의 현실적합성을 확인하기 위해 2000년에서 2008년 한국 온라인 쇼핑산업의 발전과정을 재구성하고 각 기업의 매출자료 및 총매출액을 검토하여 현재 진행 중인 온라인 유통업의 동적 성장과정에서 각 기업의 역량이 시장에 적용되어지는 과정을 확인하고 새로운 진화방향을 제시하고자 하였다. 이러한 과정을 통해 보았을 때, 오픈마켓의 주도를 통한 시장 발전은 한계에 봉착한 것으로 보인다. 따라서 향후 온라인 쇼핑몰은 자기 기반 역량 위에서 유통경로 구조 전반에 걸친 유통기능의 개발과 상품개발을 통해 경로구조를 통합하는 방향으로 발전할 것으로 잠정적 결론을 내릴 수 있을 것이다. 이러한 결론에 기반하여 오픈마켓에서 육성된 대형 판매자들의 전문몰화, 상장기업화를 유도하는 한편, 상품취급범위를 제한하는 다양한 정책 및 규제를 통합운영하고 조정하는 컨트롤 타워의 구축, 그리고 오픈마켓의 중소 상인육성기능을 강화하기 위한 인센티브의 구성과 같은 정책적 제안이 제시되었다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.149-155
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• 1987

Growth and development of a higher plant, or any living organism for that matter, could be defined as an orderly expression of the genome in time and space in close interaction with the environment. During differentiation and development of a tissue or organ a group of genes must be selectively turned on or turned off mainly by trans-acting regulators. In this general concept of regulation of regulation of gene expression, a DNA molecule is recognized at a specific nucleotide sequence by DNA-binding factors. Molecular biology of the regulatory factors such as hormones, and their receptors, target DNA sequences and DNA-binding proteins are well advanced. What is not clearly understood is the molecular basis of the interactions between DNA and binding factors, expecially of the usages of the dyad symmetry of the target DNA sequences and the dimeric nature of the DNA-binding proteins. A unique 3-dimensional structure of DNA has been proposed that may play an important role in the orderly expression of the gene. A foldback intercoil (FBI) DNA configuration which was originally found by electron microscopy among mtDNA molecules from pearl millet has some unique features. The FBI configuration of DNA is believed to be formed when a flexible double helix folds back and interwines in the widened major grooves resulting in a four stranded, intercoil DNA whose thickness is the same as that of double stranded DNA. More recently, the FBI structure of DNA has been also induced in vitro by a novel enzyme which was purified from pearl millet mitochondria. It has been proposed that the FBI DNA could be utillized in intramolecular recombination which leads to inversion or deletion, and in intermolecular recombination which can lead to either site-specific recombination, genetic recombination via single strand invasion, or cross strand recombination. The structure and function of DNA in 3-dimensional aspect is emphasized for better understanding orderly expression of genes during growth and development.