• 가족자원경영과 정책
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• 제14권4호
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• pp.39-56
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• 2010

The purpose of this research was to analyze the relationship between environment-friendly housing behaviors and the influential variables among consumers by focusing on housing life. This research carried out a questionnaire survey with housewives living in Seoul and the Metropolitan area using a questionnaire that was composed based on previous research. The housewives' environment-friendly housing attitudes and their resource-saving knowledge level, along with environmental education-related variables, house ownership, and the housewives' employment appeared to be significant variables in explaining the generic environment-friendly housing behaviors found in this research. Three regression equations, classified into purchasing, utilization, and disposal behaviors, were employed. The empirical results were relatively similar to those for the generic environment-friendly housing behaviors; however, each model provided somewhat different results in some specific aspects. All the environmental education-related variables appeared to be significant in explaining environment-friendly housing utilization behaviors, and were similar to the results obtained from the generic environment-friendly housing behaviors. However, the variables were limited in explaining environment-friendly housing-related disposal behaviors. The convenience, recycling ease, and family-structure variables appeared to be significant influences on environment-friendly housing-related disposal behaviors. Most empirical results of this research were consistent with those of previous studies. However, the explanatory independent variables varied, depending on the types of each domain of environment-friendly housing behaviors studied.

• BMB Reports
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• 제34권5호
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• pp.402-407
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• 2001

Based on the currently proposed toxicity model for the different Bacillus thuringiensis Cry $\delta$-endotoxins, their pore-forming activity involves the insertion of the ${\alpha}4-{\alpha}5$ helical hairpin into the membrane of the target midgut epithelial cell. In this study, a number of polar or charged residues in helix 4 within domain I of the 65-kDa dipteranactive Cry11A toxin, Lys-123, Tyr-125, Asn-128, Ser-130, Gln-135, Arg-136, Gln-139 and Glu-141, were initially substituted with alanine by using PCR-based directed mutagenesis. All mutant toxins were expressed as cytoplasmic inclusions in Escherichia coli upon induction with IPTG. Similar to the wild-type protoxin inclusion, the solubility of each mutant inclusion in the carbonate buffer, pH 9.0, was relatively low When E. coli cells, expressing each of the mutant proteins, were tested for toxicity against Aedes aegypti mosquito-larvae, toxicity was completely abolished for the alanine substitution of arginine at position 136. However, mutations at the other positions still retained a high level of larvicidal activity Interestingly, further analysis of this critical arginine residue by specific mutagenesis showed that conversions of arginine-136 to aspartate, glutamine, or even to the most conserved residue lysine, also abolished the wild-type activity The results of this study revealed an important determinant in toxin function for the positively charged side chain of arginine-136 in helix 4 of the Cry11A toxin.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 추계학술대회
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• pp.1340-1345
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• 2003

In this paper, an integrated optimal design software system for structural components has been developed which interfaces existing commercial codes for CAD, CAE and Optimization. They include specialized optimal design software codes such as iSIGHT and VisualDOC, optimization module imbedded in CAD software developed by CAD developers, and optimal design software systems based on API of commercial CAD software. The advantages of the CAD imbedded optimal design approach and those of specialized optimal design software are taken to develop the system. The user defines optimal design formulation in the user interface for problem definition in the CAD control stage, where design variables are directly selectable from the CAD model and various properties and performance functions defined. The commercial CAD codes, Open I-DEAS are used for the development. The resulting software is minimally connected to CAD and CAE systems while keeping maximum independence from each other. This assures flexibility and freedom for problem definition. Fatigue life optimization is taken as a nontrivial application area. As a specific example, the shape design of a knuckle part of an automobile is performed, where the minimum fatigue life over the material domain in terms of the number of cycles of a curb strike are maximized under the constraint of not exceeding the current mass. The fatigue life has been improved by four times of the initial life. The developed software is illustrated to maintain the advantages of existing optimal design software systems while improving independency and flexibility.

• International Journal of Fluid Machinery and Systems
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• 제2권4호
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• pp.392-399
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• 2009

The paper concerns the description of the step by step development process of the new fixed blade runner called "Mixer" suitable for the uprating of the Francis turbines units installed at the older low head hydropower plants. In the paper the details of hydraulic and mechanical design are presented. Since the rotational speed of the new runner is significantly higher then the rotational speed of the original Francis one, the direct coupling of the turbine to the generator can be applied. The maximum efficiency at prescribed operational point was reached by the geometry optimization of two most important components. In the first step the optimization of the draft tube geometry was carried out. The condition for the draft tube geometry optimization was to design the new geometry of the draft tube within the original bad draft tube shape without any extensive civil works. The runner blade geometry optimization was carried out on the runner coupled with the draft tube domain. The blade geometry of the runner was optimized using automatic direct search optimization procedure. The method used for the objective function minimum search is a kind of the Nelder-Mead simplex method. The objective function concerns efficiency, required net head and cavitation features. After successful hydraulic design the modal and stress analysis was carried out on the prototype scale runner. The static pressure distribution from flow simulation was used as a load condition. The modal analysis in air and in water was carried out and the results were compared. The final runner was manufactured in model scale and it is going to be tested in hydraulic laboratory. Since the turbine with the fixed blade runner does not allow double regulation like in case of full Kaplan turbine, it can be profitably used mainly at power plants with smaller changes of operational conditions or in case with more units installed. The advantages are simple manufacturing, installation and therefore lower expenses and short delivery time for turbine uprating.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.77-80
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• 2001

A variety of different methods to generate diverse proteins, including random mutagenesis and recombination, are currently available, and most of them accumulate the mutations on the target gene of a protein, whose sequence space remains unchanged. On the other hand, a pool of diverse genes, which is generated by random insertions, deletions, and exchange of the homologous domains with different lengths in the target gene, would present the protein lineages resulting in new fitness landscapes. Here we report a method to generate a pool of protein variants with different sequence spaces by employing green fluorescent protein (GFP) as a model protein. This process, designated functional salvage screen (FSS), comprises the following procedures： a defective GFP template expressing no fluorescence is firstly constructed by genetically disrupting a predetermined region(s) of the protein, and a library of GFP variants is generated from the defective template by incorporating the randomly fragmented genomic DNA from E. coli into the defined region(s) of the target gene, followed by screening of the functionally salvaged, fluorescence-emitting GFPs. Two approaches, sequence-directed and PCR-coupled methods, were attempted to generate the library of GFP variants with new sequences derived from the genomic segments of E. coli. The functionally salvaged GFPs were selected and analyzed in terms of the sequence space and functional property. The results demonstrate that the functional salvage process not only can be a simple and effective method to create protein lineages with new sequence spaces, but also can be useful in elucidating the involvement of a specific region(s) or domain(s) in the structure and function of protein.

• 한국디지털건축인테리어학회논문집
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• 제12권3호
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• pp.49-58
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• 2012

In Regarding urban geo-spatial information visualization in this study, visualization of physical attribute information is mostly related to the representation of spatial domain and physical objects, and visualization of non-physical attribute information has to do with visualizing citizens and their activity information in the physical environment. In particular, a citizen, one of the non-physical information attributes, is characterized by a constant mobility in the physical environment, and thus visualization of his/her activity information is vital in conveying information because this information may not be depicted with any given, fixed figures. In consideration of this difficulty, this study integrates interrelationship among urban space, human needs, social relations, and lifestyles into a 'high-tech' service where experience in time and space is possible. To analyze the web-service and visualization cases, the study has selected visualization indicators consisting of information representation objective, information representation means, and information representation result. The results of the case analysis through specific indicators set a range in information representation objective, means, and result, thereby forming a user-friendly environment in which representational results are readily available to users, and ultimately expanding the users' selection range coming with a variety of options. This study also assumes that a wide range of service provision is needed to reflect users' needs in developing urban geo-spatial service model, and the display of its results should visualize users' satisfaction to construct the best information needed to users.

• 한국음향학회지
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• 제24권4호
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• pp.210-216
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• 2005

본 논문에서는 잡음에 강인한 음성인식을 위하여 고유공간에 기반을 둔 새로운 특징 정규화 기법을 제안한다. 일반적으로 평균과 분산의 정규화 (MVN)는 켑스트럼 상에서 수행된다. 그러나 최근에 고유공간을 이용한 MVN기법이 소개되었고, 그 고유공간 정규화 기법에서는 하나의 고유공간을 이용하였다. 이 과정에는 켑스트럼 상의 특징 벡터를 선형 주성분 분석 (PCA)행렬을 통하여 고유공간으로 변환시킨 후 MVN을 수행하는 과정이 포함된다. 이 방법에서는 전체 39차의 특징분포를 하나의 고유공간으로 표현하였다. 그러나 이 기법의 경우 전체 특징 분포를 표현함에 세밀함이 떨어지기 때문에 더욱 세밀한 분포의 표현을 위해 본 논문에서는 static 특징, 1차 미분 계수, 2차 미분계수에 각각 유일하고 독립적인 분리된 고유공간을 적용하는 것을 제안하였다. 또한 고유공간에서 정규화 된 훈련 데이터를 이용하여 모델을 만든다. 마지막으로 훈련 데이터의 분포와 잡음환경에서의 테스트 데이터의 분포 특성의 차이를 줄이기 위해 켑스트럼 상에서의 회전 기법을 적용시킨다. 그 결과, 기본적인 고유공간 정규화 기법보다 향상된 성능을 얻을 수 있었다.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.50-50
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• 2003

We have identified and analysed a putative response regulator two-component gene (CaSKN7) from Candida albicans and its encoding protein (CaSkn7). CaSKN7 has an open reading frame of 1677bp. CaSKN7 encodes a 559 amino acid protein (CaSkn7) with an estimated molecular mass of 61.1 kDa. CaSKN7 is a homologue of a Saccharomyces cerevisiae SKN7 that is the regulator involved in the oxidative stress response. To study the role of CaSKN7, we constructed a CAI4-derived mutant strain carrying a homozygous deletion of the CaSKN7 gene. In the caskn7 disruptant cells, the formation of germ tube require shorter time than that in the congenic wild-type strain but the growth of mycelium delayed in liquid media. In contrast, the caskn7 disruptant cells attenuate the differentiation in solid media and the virulence in mouse model system. Expression level of hypha-specific and virulence genes - HYR1, ECE1, HWP1, and ALS1 - in the caskn7 disruptant cells increased as compared with that in the congenic wild-type strain in 10％ serum YPD. Skn7 in 5. cerevisiae was found to bind the HSE element from the SSA promoter, Also, CaSkn7 contains heat shock factor DNA-binding domain and the promoters of these genes have HSE-like sties. Therefore these results show that CaSKN7 regulate the differentiation and virulence of C. albicans.

• 한국인터넷방송통신학회논문지
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• 제15권6호
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• pp.135-140
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• 2015

CPS는 다양한 임베디드 시스템들을 복합적으로 모델링 및 시뮬레이션 한다. 이때 다양한 종류의 모델이 사용되기 때문에 시뮬레이션에 사용되는 데이터들에 대한 체계적인 관리 위해 SEDRIS를 적용한다. SEDRIS는 특정 도메인 환경에서 개발된 환경데이터들을 재사용 및 상호운영 가능하기 때문에 비용과 시간을 절감할 수 있다. 기존 시뮬레이터와 SEDRIS를 연동 위해, 시뮬레이터의 지형 표현에 사용되는 높이맵을 SEDRIS의 레스터 데이터 표현방법으로 변환하여 입력만이 가능하다. 이 문제를 해결위해, 높이맵 표현을 위해 사용되는 RAW파일을 폴리곤 데이터로 변환하는 방법을 제안한다. 제안한 방법은 두 가지 이점을 제공한다. 첫 번째로 SEDRIS 내부에 환경데이터 표현이 가능해진다. 두 번째는 그림파일처럼 보이는 지형을 뷰어로 확인 가능하다. 즉, SEDRIS 비전문가도 지형 환경 데이터를 구축이 가능하다.

• 물과 미래
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• 제27권3호
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• pp.115-121
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• 1994

하구수리계에 있어서 점착성류사의 운동은 응결과 응집(aggregation) 현상에 의해 크게 좌우되며, 이들 현상에 의하여 의결된 입자들의 크기, 모양, 강도는 그 퇴적율과 그리고 부유류사와 오염물질의 변동과정에 영향을 미친다. 본 연구에서는 Brownian 운동에 의해 지배되는 이동성 부유영역과 중력에 의해 지배되는 정체성 부유영역으로 분리하여 점착성입자들의 응집과 응결과정을 모의하였다. 이동성 부유영역에 있어서는 Smoluchowski 모형중 입자들의 무작위 회전을 이용한 최대연쇄 모형으로 응집물질의 사영들을 도출하였으며, 그 회전반경에 의해 사영들의 쪽거리 차원을 구하였다. 저에성 부유영역에서의 응집물질(Brownian 영역을 벗어나 침강하는 응집물질)는 비구형 입자들의 침강으로 간주되어진다. 최종 침강속도 차이로 이들 응집물들은 재차 충돌, 결합하여 보다 큰 응집 입자들을 형성하며, 이들 최종 응집입자들의 사영이 모의되어진다. 최대 Feret's 직경과 Minkowski's sausage logic 개념들을 이용하여 모의되어진 사영들의 둘레 길이에 대하여 쪽거리 차원들을 구하였다.