• Title/Summary/Keyword: diversity methods

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Use of Terminal Restriction Length Polymorphism (T-RFLP) Analysis to Evaluate Uncultivable Microbial Community Structure of Soil

  • Chauhan, Puneet Singh;Shagol, Charlotte C.;Yim, Woo-Jong;Tipayno, Sherlyn C.;Kim, Chang-Gi;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.1
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    • pp.127-145
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    • 2011
  • Various environmental ecosystems are valuable sources for microbial ecology studies, and their analyses using recently developed molecular ecological approaches have drawn significant attention within the scientific community. Changes in the microbial community structures due to various anthropogenic activities can be evaluated by various culture-independent methods e.g. ARISA, DGGE, SSCP, T-RFLP, clone library, pyrosequencing, etc. Direct amplification of total community DNA and amplification of most conserved region (16S rRNA) are common initial steps, followed by either fingerprinting or sequencing analysis. Fingerprinting methods are relatively quicker than sequencing analysis in evaluating the changes in the microbial community. Being an efficient, sensitive and time- and cost effective method, T-RFLP is regularly used by many researchers to access the microbial diversity. Among various fingerprinting methods T-RFLP became an important tool in studying the microbial community structure because of its sensitivity and reproducibility. In this present review, we will discuss the important developments in T-RFLP methodology to distinguish the total microbial diversity and community composition in the various ecosystems.

Effects of cutting and sowing seeds of native species on giant ragweed invasion and plant diversity in a field experiment

  • Byun, Chaeho;Choi, Ho;Kang, Hojeong
    • Journal of Ecology and Environment
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    • v.44 no.4
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    • pp.256-263
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    • 2020
  • Background: Ambrosia trifida is a highly invasive annual plant, but effective control methods have not been proposed. Among various eradication methods, cutting is a simple measure to control invasive plants, and sowing seeds of native plants may effectively increase biotic resistance to invasion. In this study, we conducted a field experiment with two treatments: cutting and sowing seeds of six native or naturalized plants. Results: We found a significantly lower A. trifida abundance after cutting than in the control (77% decrease). Sowing seeds of native species did not provide any additional benefit for the control of A. trifida, but increased the importance values and diversity of other native vegetation. The abundance of A. trifida was negatively correlated with that of other plant taxa based on plant cover, biomass, and density. However, biotic resistance of sown plants was not effective to control invasion because A. trifida was so competitive. Conclusions: We concluded that cutting is an effective measure to control Ambrosia trifida while sowing seeds of native plants can increase native plant diversity.

A Study on Diverse Designs of Furniture Presented as Graduation Projects by Graduates of Local Universities - Focusing on painting methods for wooden surface -

  • Choi, Ki;Lee, Jae-Young
    • Journal of the Korea Furniture Society
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    • v.19 no.4
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    • pp.252-259
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    • 2008
  • High glossy painting and UV lithographic painting as painting methods for wooden furniture have played a positive role in enhancing the diversity of furniture design, which has been proved by the analysis on the relationship between the change in the diversity of design and painting methods of graduation projects (wooden furniture) presented by graduates of local universities, as a focus of this article. Since the development of new painting methods has the potential to develop aesthetic characteristics of wooden furniture, many design experiments are needed to improve decorative features of furniture, such as the application of high glossy painting and UV lithographic painting.

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The Impact of the Diversity Management on the Workforce Composition and Financial Performance (다양성관리가 조직인력구성과 재무성과에 미치는 영향)

  • Sung, Sang-Hyeon;Kim, Dae-Lyong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.1
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    • pp.110-124
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    • 2011
  • Even though Korea has long experienced single race/ethnic demographic composition, the recent rapid change of demographic diversity will have powerful impact on the HRM(human resource management) in the future. In this research we find the influence of the diversity friendly HRM to the human resource practices using the data of WPS(Workplace Panel Survey) of Korea Labor Institute. If the training for women is high, the density of the woman increased. The performance pay is introduced, then the density of the disabled person would be increased. The foreign workforce was also increased if the company used more selection methods. But the HRM practices such as talent management, yearly-based compensation systems, and appraisal program which has a tendency to reduce diversity have no significant influence to the financial performance. This findings imply that the management team should introduce the effective HRM systems to manage diverse human resources after considering the organizational culture and business environment. This research intends to find the diversity friendly HRM practices and hope to help the efforts of the management team to find effective management methods.

Y-Single Nucleotide Polymorphisms Diversity in Chinese Indigenous Horse

  • Han, Haoyuan;Zhang, Qin;Gao, Kexin;Yue, Xiangpeng;Zhang, Tao;Dang, Ruihua;Lan, Xianyong;Chen, Hong;Lei, Chuzhao
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.8
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    • pp.1066-1074
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    • 2015
  • In contrast to high genetic diversity of mitochondrial DNA (mtDNA), equine Y chromosome shows extremely low variability, implying limited patrilines in the domesticated horse. In this study, we applied direct sequencing and restriction fragment length polymorphism (RFLP) methods to investigate the polymorphisms of 33 Y chromosome specific loci in 304 Chinese indigenous horses from 13 breeds. Consequently, two Y-single nucleotide polymorphisms (SNPs) (Y-45701/997 and Y-50869) and one Y-indel (Y-45288) were identified. Of those, the Y-50869 (T>A) revealed the highest variation frequency (24.67%), whereas it was only 3.29% and 1.97% in Y-45288 (T/-) and Y-45701/997 (G>T) locus, respectively. These three mutations accounted for 27.96% of the total samples and identified five Y-SNP haplotypes, demonstrating genetic diversity of Y chromosome in Chinese horses. In addition, all the five YSNP haplotypes were shared by different breeds. Among 13 horse breeds analyzed, Balikun horse displayed the highest nucleotide diversity (${\pi}=5.6{\times}10^{-4}$) and haplotype diversity (h = 0.527), while Ningqiang horse showed the lowest nucleotide diversity (${\pi}=0.00000$) and haplotype diversity (h = 0.000). The results also revealed that Chinese horses had a different polymorphic pattern of Y chromosome from European and American horses. In conclusion, Chinese horses revealed genetic diversity of Y chromosome, however more efforts should be made to better understand the domestication and paternal origin of Chinese indigenous horses.

An Overview of Different Techniques on the Microbial Community Structure, and Functional Diversity of Plant Growth Promoting Bacteria

  • Kim, Kiyoon;Islam, Rashedul;Benson, Abitha;Joe, Manoharan Melvin;Denver, Walitang;Chanratan, Mak;Chatterjee, Poulami;Kang, Yeongyeong;Sa, Tongmin
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.2
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    • pp.144-156
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    • 2016
  • Soil is a dynamic biological system, in which it is difficult to determine the composition of microbial communities. Knowledge of microbial diversity and function in soils are limited because of the taxonomic and methodological limitations associated with studying the organisms. In this review, approaches to measure microbial diversity in soil were discussed. Research on soil microbes can be categorized as structural diversity, functional diversity and genetic diversity studies, and these include cultivation based and cultivation independent methods. Cultivation independent technique to evaluate soil structural diversity include different techniques such as Phospholipid Fatty Acids (PLFA) and Fatty Acid Methyl Ester (FAME) analysis. Carbon source utilization pattern of soil microorganisms by Community Level Physiological Profiling (CLPP), catabolic responses by Substrate Induced Respiration technique (SIR) and soil microbial enzyme activities are discussed. Genetic diversity of soil microorganisms using molecular techniques such as 16S rDNA analysis Denaturing Gradient Gel Electrophoresis (DGGE) / Temperature Gradient Gel Electrophoresis (TGGE), Terminal Restriction Fragment Length Polymorphism (T-RFLP), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP) / Amplified Ribosomal DNA Restriction Analysis (ARDRA) and Ribosomal Intergenic Spacer Analysis (RISA) are also discussed. The chapter ends with a final conclusion on the advantages and disadvantages of different techniques and advances in molecular techniques to study the soil microbial diversity.

MIMO Ad Hoc Networks: Medium Access Control, Saturation Throughput, and Optimal Hop Distance

  • Hu, Ming;Zhang, Junshan
    • Journal of Communications and Networks
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    • v.6 no.4
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    • pp.317-330
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    • 2004
  • In this paper, we explore the utility of recently discovered multiple-antenna techniques (namely MIMO techniques) for medium access control (MAC) design and routing in mobile ad hoc networks. Specifically, we focus on ad hoc networks where the spatial diversity technique is used to combat fading and achieve robustness in the presence of user mobility. We first examine the impact of spatial diversity on the MAC design, and devise a MIMO MAC protocol accordingly. We then develop analytical methods to characterize the corresponding saturation throughput for MIMO multi-hop networks. Building on the throughout analysis, we study the impact of MIMO MAC on routing. We characterize the optimal hop distance that minimizes the end-to-end delay in a large network. For completeness, we also study MAC design using directional antennas for the case where the channel has a strong line of sight (LOS) component. Our results show that the spatial diversity technique and the directional antenna technique can enhance the performance of mobile ad hoc networks significantly.

Diversity and Conservation of Korean Marine Fishes (한국 해산어류의 종다양성 및 보전)

  • Kim, Jin-Koo
    • Korean Journal of Ichthyology
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    • v.21 no.sup1
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    • pp.52-62
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    • 2009
  • Environmental differences of each sea around the Korean Peninsula in terms of factors including topography and complexity of sea current may influence species and genetic diversity of marine fishes. Fish are naturally abundant in the frontal area where various currents or water masses meet. However, this food resource is prone to human overexploitation, threatening the marine ecosystem. New fisheries resources management strategies are needed. Such strategies require information about population structure obtained through morphological and genetic methods.

A Study on Effects of Antenna Diversity in Doppler Spread Environments (도플러 확산 환경에서의 안테나 다이버시티 효과에 관한 연구)

  • Lee Jong-Gil
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.9 no.3
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    • pp.510-515
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    • 2005
  • Doppler spread can occur due to the relative movement of transmitter and receiver. The Doppler frequency shift varies as the relative location and the velocity of transceivers change. This Doppler spread may seriously degrade the performance of OFDM system which is considered to be very efficient for multimedia wireless communication. Therefore, applying the method of receiver diversity, we analyze the degree of BER improvement in Doppler spread environments to investigate the effectiveness of the chosen methods according to various wireless channels.

Bacterial Diversity in a Korean Traditional Soybean Fermented Foods (Doenjang and Ganjang) by 16S rRNA Gene Sequence Analysis

  • Cho, Kye-Man;Seo, Weon-Taek
    • Food Science and Biotechnology
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    • v.16 no.2
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    • pp.320-324
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    • 2007
  • The bacterial diversity in Korean soybean-fermented foods was investigated using a PCR-based approach. 16S rRNA sequences were amplified and cloned from two different soybean-fermented foods such as doenjang (soybean paste), and ganjang (soybean sauce). Staphylococcus equorum (60.6%), Tetragenococcus halophila (21.2%), Leuconostoc mesenteroides (9.1%), Lactobacillus sakei (6.1%), and Bacillus subtilis (3.0%) were detected among clones isolated from soybean paste samples and Halanaerobium sp. (37.5%), Halanaerobium fermentans (37.5%), T. halophila (12.5%), Staphylococcus sp. (6.3%), S. equorum (3.1%), and B. subtilis (3.1%) were detected among clones isolated from soybean sauce. Our approach revealed different bacterial distributions and diversity from those previously obtained using culture-dependent methods.