• Title/Summary/Keyword: disinfection

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Clinical short-term effects of full-mouth disinfection (Full-mouth disinfection의 단기간의 임상적 효과)

  • Lee, Shin-Hwa;Kim, Ok-Su;Kim, Young-Joon;Chung, Hyun-Ju
    • Journal of Periodontal and Implant Science
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    • v.37 no.3
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    • pp.613-624
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    • 2007
  • Full-mouth disinfection (Fdis) completes the entire scaling and root planing (SRP) in one stage within 24 hours for the prevention of microbial recolonization from untreated sites and ecological niches. The aim of this study is to compare the clinical short-term effects of modified Fdis with those of the conventional SRP in the therapy of moderate and severe chronic periodontitis. Modified Fdis group (5 patients) received the entire SRP within 24 hours using chlorhexidine solution (0.1%) and conventional SRP group (5 patients) received SRP per quadrant at one-week intervals. Clinical parameters were measured at baseline, one month and three months after both therapies. The results of this case report were as follows: 1. There were considerable decreases in sulcus bleeding index and plaque index one month after Fdis. 2. The mean probing depth of single-rooted teeth decreased more in Fdis group than conventional SRP group after therapy and, that of multi-rooted teeth decreased similarly in both groups. 3. The mean probing depth decreased 1.77mm in case of initial probing depth of 4-6mm and it decreased 4.13mm in case of initial probing depth of ${\geq}$ 7mm three months after Felis. 4. There were the smaller increases in gingival recession together with the larger gains in attachment in Fdis group than conventional SRP group after three months. Within the limitations of this study, one could conclude that Fdis has beneficial clinical effects in the treatment of moderate and severe chronic periodontitis and further research would be helpful including more subjects during a longer period to confirm the beneficial long-term effects of Fdis.

Occurrence of Disinfection By-Products and Distribution in Drinking Water

  • In, Chi-Kyung;Lee, Jung-Ho;Lee, In-Sook
    • Proceedings of the Korean Environmental Health Society Conference
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    • 2005.12a
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    • pp.103-114
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    • 2005
  • Chlorine disinfection has been used in drinking water supply to disinfect the water-borne microbial disease which may cause to serious human disease. As Chlorination is still the least costly, relatively easy to use, chlorination is the primary means to disinfect portable water supplies and control bacterial growth in the distribution system. However, chlorine also reacts with natural organic matter (NOM), which presents in nearly all water sources, and then produces disinfection by-product (DBps), which may have adverse health effects. Although the existent DBPs have been reported in drinking water supplies, it is not feasible to predict the levels of the various DBPs due to the complex chemistry reaction involved. The objectives of this study were to investigate seasonal variation of DBPs formation and difference of DBPs concentration in the plant to tap water. The average concentration of THMs was 20.04 ${\mu}g/{\ell}$, HAAs 8-15 ${\mu}g/{\ell}$, HANs 2-4.5 ${\mu}g/{\ell}$ respectively. Distant variation of DBPs formation is that THMs concentration increase by 17% at 2 km point from the plant and by 28% at 7 km and HAAs, HANs also increase each by 16%, 32%, at 2 km from the plant and 35%, 56%, at 7 km. DBPs increase in water supply pipe continually. The seasonal occurrence of DBPs is that in May and August DBPs concentration is very high then in March, in May DBPs concentration is highest. The temperature is main factor of DBPs formation, precursor also. Precursor which was accumulated for winter flowed into the raw water by flooding in spring and summer and produced DBPs. Therefore for the supply of secure drinking water, it is required to protect precursor of flowing into raw water and to add to BCAA and DBAA to drinking water standards.

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The Antibiotic Resistant Gene Pollutant Controls using Chlorine or Ozone disinfection (염소 또는 오존을 이용한 항생제 내성 유전오염물질 제어)

  • Kim, Sung-Pyo;Rhu, Dae-Whan;Oh, Jun-Sik;Cho, Yun-Chul
    • Journal of Wetlands Research
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    • v.13 no.3
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    • pp.697-705
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    • 2011
  • The aim of this study was to examine ozonation disinfection efficiency for Escherichia coli DH5alpha removal, containing the multi-resistance plasmid pB10 as well as chlorination disinfection efficiency. In addition, plasmid pB10 removal rates were estimated by ozonation and chlorination. The removal efficiency of pB10 via ozonation was about 2 to 4 times higher than chlorination. High removal efficiency of pB10 is likely due to OH radical produced during ozonation. These results suggest that integration of advanced oxidation process such as ozonation (or photocatalytic oxidation) with conventional disinfection such as chlorination may be needed for effective control of antibiotic resistant bacteria and genetic materials.

A Study on Microbial Contamination and Disinfection of Ultrasonic Probe in Metropolitan Area (수도권 지역의 초음파 프로브의 미생물 오염도와 소독에 관한 연구)

  • Lee, Hyun Kyung;Kim, Sam Soo;Heo, Yeong Cheol;Han, Dong Kyoon
    • Journal of radiological science and technology
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    • v.41 no.5
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    • pp.427-435
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    • 2018
  • There was a shortage of research reports on sterilization criterion and contamination of ultrasonic probes. Therefore, in this study, we were going to provide a basic study to measure the level of microbial contamination in ultrasonic probes and to investigate the radiographer's awareness of infection. After the scan, samples were collected from the rubber part of the probe by opening a sterile swab (Transport Medium AM608-1S) for medical bacteria collection with the remaining gel removed with a paper towel. Also, the collected samples of bacteria were grown for seven days and then the laboratory was analyzed. Among the total 29 types of microorganisms, Micrococcus luteus 21(26%), Moraxella species 16(20%), Coagulase negative staphylococcus 8(10%), Bacillus species 5(7%), Bicillus circulans 3(5%), Acinetobacter lwoffii 2(2%), and 1 other Candida parapsilosis (1%) a number of bacteria and fungus, was detected. In a disinfectant experiment using LuciPac Pen on the Lumitester PD-30s, we cultured the rubber part of the probe two to three times to measure the bacteria. Bacteria decreased to 97% with Aquanax (alkaline reduced water 100%), 99% with Klarion wash (0.01% sodium hydroxide), 94% with Klarion disinfection (0.01% nitrous acid water), Sterilization was best with Klarion wash (0.01% sodium hydroxide). Therefore, guidelines for cleaning and disinfection of ultrasonic probes was required, and further development of probe-only disinfectants is required.

Disinfection Effects of Chlorine and Monochloramine on Campylobacter jejuni (Campylobacter jejuni에 대한 염소 및 Monochloramine의 살균효과)

  • 윤만석;오학식;김치경
    • Microbiology and Biotechnology Letters
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    • v.17 no.6
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    • pp.539-544
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    • 1989
  • Campylobacter jejuni, bacterial agent causing human diarrhea, were studied for their disinfection effects with chlorine and monochloramine. The cells treated with the chemical agents were observed by scanning electron microscopy to know their morphological and structural changes. The proteins and DNA in the chemical-treated cells were also examined by gel electrophoresis for structural changes. When C. jejuni were chlorinated at concentrations of 0.5 and 1.0 mg/l for 15 minutes, the organisms were disinfected by 4 and 6 log, respectively. Those disinfection effects were higher at acidic pH, while lowered at neutral and alkaline values of pH. The effects of monochloramine were lower than those of chlorine at the game concentration for the same period of reaction time. The shapes of C. jejuni cells treated with the agents appeared to be deformed from spiral rod into spherical forms, showing some destruction in surface structure of the cells. Some of the proteins and DNA of the chlorinated cells did not appear in the gel electrophoresis when the chlorination was at concentration of 10 mg/l or higher.

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Comparative Characterization on Bacterial Disinfection Using Trichloroisocyanuric Acid, Bromochlorodimethylhydantoin, and Calcium Hypochlorite (삼염화이소시아눌산, 브룸염화히단토인, 및 칼슘하이포클로라이트의 농도 및 접촉시간에 따른 미생물 사멸특성)

  • Oh, Se-Hyun;Yoo, Hyun-Duk;Lee, Han-Na;Kim, Eung-Soo
    • Microbiology and Biotechnology Letters
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    • v.35 no.4
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    • pp.334-338
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    • 2007
  • The disinfection effects of trichloroisocyanuric acid (TICA), calcium hypochlorite (CH), and Bromochlorodimethylhydantoin (BCDMH) on various bacteria in aqueous suspension were comparatively characterized at various concentrations and exposure times of each disinfectant. When various bacteria cells ($10^8\;CFU/ml$) including Escherichia coli, Pseudomonas aeroginosa, Enterococcus faecalis, Bacillus cereus, Legionella pneumophila, and Staphylococcus aureus were exposed with a solution containing 8 ppm each of TICA, a 99% of the initial cells were killed in 60 sec, 368 sec, 372 sec, 506 sec, 812 sec, and 909 sec, respectively. In addition, the minimum exposure time required to kill 99% of E. coli ($10^8\;CFU/ml$) with 8 ppm of each TICA, BCDMH and CH was measured at 60 sec, 114 sec, and 7,100 sec, respectively. These comparative studies demonstrate that disinfection efficacy is highly variable depending on microbial species as well as disinfectant type, concentration, and exposure time.

Application of UV Technology for Surface Disinfection (표면 살균을 위한 UV 기술의 적용)

  • Cho, Min;Jeong, Woo-Dong;Yoon, Je-Yong
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.9
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    • pp.1020-1026
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    • 2007
  • The control of pathogenic microorganisms is important issue in human environments, especially in surface area. However, surface disinfection has not been fully researched. In this study, the surface disinfection under UV irradiation was performed to investigate the quantitative kinetics for Bacillus subtilis spore inactivation in several experimental conditions, such as light intensity, temperature and surface roughness. This study reports that UV light would apparently inactivates the microorganisms and the required IT value for 2 log (99%) Bacillus subtilis spore inactivation was found to be 14.5 $mJ/cm^2$ in plain surface, as predicted by the Delayed Chick-Watson model. When UV was irradiated, there were no significant temperature effects. However, the experimental result shows that the more increased IT values are required at larger surface roughness.

The clinical effects of modified full-mouth disinfection in the treatment of moderate to severe chronic periodontitis patients

  • Lee, Shin-Hwa;Kim, Young-Joon;Chung, Hyun-Ju;Kim, Ok-Su
    • Journal of Periodontal and Implant Science
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    • v.39 no.sup2
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    • pp.239-251
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    • 2009
  • Purpose: Full-mouth disinfection enables to reduce the probability of cross contamination from untreated pockets to treated ones, for completing the entire SRP under local anesthesia with chlorhexidine as a mouth wash in two visits within 24 hours. This study aimed to compare the clinical effects of modified full-mouth disinfection (Fdis) after 6 months with those of conventional SRP (cSRP). Methods: Thirty non-smoking chronic periodontitis subjects were randomly allocated two groups. The Fdis group underwent the entire SRP under local anesthesia in two visits within 24 hours, a week after receiving supragingival scaling. A chlorhexidine (0.1%) solution was used for rinsing and subgingival irrigation for Fdis. The cSRP group received SRP per quadrant under local anesthesia at one-week intervals, one week after they had received scaling. Clinical parameters were recorded at baseline, after 1, 3 and 6 months. Results: There are significant (P<0.05) decreases in the sulcus bleeding index, and plaque index, and the increases in gingival recession were significantly smaller with Fdis after six months compared with cSRP. There was significant improvement in the probing depth and clinical attachment level for initially medium-deep pockets (4-6mm) after Fdis compared with cSRP. Multi-rooted teeth showed significantly larger attachment gain up to six months after Fdis. Single-rooted teeth showed significantly more attachment gain, 1 and 6 months after Fdis. Conclusions: Fdis has more beneficial effects on reducing gingival inflammation, plaque level, probing depth, gingival recession and improving clinical attachment level over cSRP.

Application of Inactivation Model on Phytophthora Blight Pathogen (Phytophthora capsici) using Plasma Process (플라즈마 공정을 이용한 고추역병균(Phytophthora capsici) 불활성화 모델의 적용)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Science International
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    • v.24 no.11
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    • pp.1393-1404
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    • 2015
  • Ten empirical disinfection models for the plasma process were used to find an optimum model. The variation of model parameters in each model according to the operating conditions (first voltage, second voltage, air flow rate, pH, incubation water concentration) were investigated in order to explain the disinfection model. In this experiment, the DBD (dielectric barrier discharge) plasma reactor was used to inactivate Phytophthora capsici which cause wilt in tomato plantation. Optimum disinfection models were chosen among ten models by the application of statistical SSE (sum of squared error), RMSE (root mean sum of squared error), $r^2$ values on the experimental data using the GInaFiT software in Microsoft Excel. The optimum models were shown as Log-linear+Tail model, Double Weibull model and Biphasic model. Three models were applied to the experimental data according to the variation of the operating conditions. In Log-linear+Tail model, $Log_{10}(N_o)$, $Log_{10}(N_{res})$ and $k_{max}$ values were examined. In Double Weibull model, $Log_{10}(N_o)$, $Log_{10}(N_{res})$, ${\alpha}$, ${\delta}_1$, ${\delta}_2$, p values were calculated and examined. In Biphasic model, $Log_{10}(N_o)$, f, $k_{max1}$ and $k_{max2}$ values were used. The appropriate model parameters for the calculation of optimum operating conditions were $k_{max}$, ${\alpha}$, $k_{max1}$ at each model, respectively.

The Microbial Contamination and Effective Control Method of Dental Unit Water System (치과용 유니트 수계의 미생물 오염 및 효과적인 관리 방법)

  • Yoon, Hye Young;Lee, Si Young
    • Journal of dental hygiene science
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    • v.15 no.4
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    • pp.383-392
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    • 2015
  • Dental chair unit (DCU) is the most essential equipment for the dental treatment in dentistry. DCU output water is used for various applications during dental treatment. DCU output water must be clean at the same level as drinking water since patients and dental staff are regularly exposed to water and aerosols generated from the DCU. Many studies demonstrated that DCU output water is frequently contaminated with microorganisms including opportunistic pathogen such as Legionella and Pseudomonas species. Thus, DCU output water may be a potential source of infection. In order to reduce microbial contamination levels in DCU output water, periodic management and continuous disinfection are necessary. Currently, there are a variety of disinfection methods for managing DCU output water and its efficacy is also diverse. We reviewed the level of microbial contamination, clinical implications of contaminated DCU output water and the various DCU disinfection methods.