• The Plant Pathology Journal
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• 제34권3호
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• pp.163-170
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• 2018

Strawberry Fusarium wilt disease, caused by Fusarium oxysporum f. sp. fragariae, is the most devastating disease in strawberry production. The pathogen produces chlamydospores which tolerate against harsh environment, fungicide and survive for decades in soil. Development of detection and quantification techniques are regarded significantly in many soilborne pathogens to prevent damage from diseases. In this study, we improved specific-quantitative primers for F. oxysporum f. sp. fragariae to reveal correlation between the pathogen density and the disease severity. Standard curve $r^2$ value of the specific-quantitative primers for qRT-PCR and meting curve were over 0.99 and $80.5^{\circ}C$, respectively. Over pathogen $10^5cfu/g$ of soil was required to cause the disease in both lab and field conditions. With the minimum density to develop the wilt disease, the pathogen affected near 60% in nursery plantation. A biological control microbe agent and soil solarization reduced the pathogen population 2-fold and 1.5-fold in soil, respectively. The developed F. oxysporum f. sp. fragariae specific qRT-PCR protocol may contribute to evaluating soil healthiness and appropriate decision making to control the disease.

• Asian Pacific Journal of Cancer Prevention
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• 제14권3호
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• pp.2037-2042
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• 2013

Background: Different methods of diagnosis have been found to be inefficient in terms of screening and early diagnosis of lung cancer. Cancer cells produce proteins whose serum levels may be elevated during the early stages of cancer development. Therefore, those proteins may be recognized as potential cancer markers. The aim of this study was to differentiate healthy individuals and lung cancer cases by analyzing their serum protein profiles and evaluate the efficacy of this method in the early diagnosis of lung cancer. Materials and Methods: 170 patients with lung cancer, 53 under high risk of lung cancer, and 47 healthy people were included in our study. Proteomic analysis of the samples was performed with the SELDI-TOF-MS approach. Results: The most discriminatory peak of the high risk group was 8141. When tree classification analysis was performed between lung cancer and the healthy control group, 11547 was determined as the most discriminatory peak, with a sensitivity of 85.5%, a specificity of 89.4%, a positive predictive value (PPV) of 96.7% and a negative predictive value (NPV) of 62.7%. Conclusions: We determined three different protein peaks 11480, 11547 and 11679 were only present in the lung cancer group. The 8141 peak was found in the high-risk group, but not in the lung cancer and control groups. These peaks may prove to be markers of lung cancer which suggests that they may be used in the early diagnosis of lung cancer.

• 한국작물학회지
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• 제35권1호
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• pp.97-107
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• 1990

The epidemiology of plant disease deals with the dynamic processes of host-pathogen interactions, which determine the prevalence and severity of the disease. Epidemic processes for most foliar diseases of plants follow a series of steps: arrival of pathogens on plant surfaces, initial infection, incubation period, latent period, sporulation, dissemination of secondary inoculum, and infectious period. These complex biological processes are influenced by the environment-Man also often interfers with these processes by altering the host and pathogen populations and the environment. Slowing or halting any of the epidemic processes can delay the development of the epidemic, so that serious losses in yield due to disease do not occur. It is generally recognized that the most effective and efficient method of minimizing disease damage is through the use of resistant cultivars, particularly when other methods such as fungicide applications are not economically feasible-Populations of plant pathogens are not genetically uniform nor are they necessarily stable. Cultivars bred for resistance to current populations of a pathogen may not be resistant in the future due to selection pressures placed on the pathogen populations. Understanding population development and genetic variability in the pathogen, and knowledge of the genetics of resistance in the plant should help in developing breeding strategies that wi1l provide effective and stable disease control through genetic resistance. In the United States, soybeans have ranked first in value of crops sold off the farm in recent years. Soybeans have been the leading U. S.

• 한국유기농업학회지
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• 제26권4호
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• pp.649-660
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• 2018

Colletotrichum acutatum에 의해 발생하는 고추탄저병을 방제하기 위해 토양 근권으로부터 분리한 미생물 중 길항효과가 있는 미생물을 선발하여 주요 작물병원균에 대한 항균활성 효과와 포장에서의 방제효과를 검정하였다. 기내 대치배양법을 이용한 길항력을 검정하였을 때, CAB13001 균주는 고추 탄저병을 일으키는 Colletotrichum acutatum을 포함한 식물 병원균 Sclerotinia cepivorum, Sclerotinia sclerotium, Botrytis cinerea에 대한 뛰어난 항균활성 효과를 보였다. 또한 풋고추를 이용한 생체 검정에서 선발균주는 고추 탄저병의 병반진전 억제효과가 우수하였으며, 포장에서의 방제가 역시 무처리 대비 82.4%로 매우 우수하였다. 이러한 결과로 볼 때 CAB13001 균주는 공기 전염을 하는 고추 탄저병의 생물적 방제제로 매우 유용할 것으로 판단된다. 한편, 본 연구에 사용된 길항미생물 CAB13001 균주는 16s rRNA gene의 계통학적 위치를 분석한 결과 Burkholderia lata로 동정하였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 춘계학술대회 논문집 전기기기 및 에너지변환시스템부문
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• pp.153-155
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• 2007

It is as result that study to apply mini magnet nerve curer in peripheral nervous system disease treatment. Design and embodied action power and Control Unit in cylinder form of magnet roof object firstly. Yielded service area about special quality of probe of roof object cylinder style and treatment area dimension and distance of treatment pulse secondly. Embody pulse forming course energy value by Probe's form by third, could embody treatment pulse by disease. Specially, through a special quality experiment, saved Damping pulse form and treatment pulse form etc. variously Lately, embodied this to aid a little in disease treatment that follow that there is no invasion that there is no stimulation by medicine development Go amplitude treatment pulse (traditional magneto-therapy of greatly great that strong.) does curative effect greatest at short time and becomes thought that demand is magnified greatly at the future in this research.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 심포지엄 논문집 정보 및 제어부문
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• pp.49-51
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• 2007

It is as result that study to apply mini magnet nerve curer in peripheral nervous system disease treatment. Design and embodied action power and Control Unit in cylinder form of magnet roof object firstly. Yielded service area about special quality of probe of roof object cylinder style and treatment area dimension and distance of treatment pulse secondly. Embody pulse forming course energy value by Probe's form by third, could embody treatment pulse by disease. Specially, through a special quality experiment, saved Damping pulse torn and treatment pulse form etc. variously. Lately, embodied this to aid a little in disease treatment that follow that there is no invasion that there is no stimulation by medicine development. Go amplitude treatment pulse (traditional magneto-therapy of greatly great that strong) does curative effect greatest at short time and becomes thought that demand is magnified greatly at the future in this research.

• 한국수학교육학회지시리즈B:순수및응용수학
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• 제31권2호
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• pp.131-158
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• 2024

This study investigates the impact of precautionary measures, such as isolating exposed individuals, wearing masks, and maintaining physical distance, on preventing infectious disease. A deterministic SEIQHRV epidemic model is employed for this purpose. The model's positivity, boundedness, disease-free, and endemic equilibrium points are identified. A sensitivity test assesses the impact of preventive measures on infected classes. Results show that a basic reproduction number less than unity drives disease eradiction, while a higher unity value encourages the adoption of preventive measures.

• Asian-Australasian Journal of Animal Sciences
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• 제33권3호
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• pp.390-397
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• 2020

Objective: The objective of this study was to measure the special expression pattern of lipid metabolism genes and investigate the molecular mechanisms underlying intramuscular fat (IMF) deposition in Longissimus dorsi muscle of Laiwu pigs. Methods: Thirty-six pigs (Laiwu n = 18; Duroc×Landrace×Yorkshire n = 18) were used for the measurement of the backfat thickness, marbling score, IMF content, and expression of lipid metabolism genes. Results: Significant correlations were found between IMF content and the mRNA expression of lipid metabolism genes. Of the 14 fat deposition genes measured, fatty acid synthase (FASN) showed the strongest correlation (r = 0.75, p = 0.001) with IMF content, and of the 6 fat removal genes, carnitine palmitoyl transferase 1B (CPT1B) exhibited the greatest negative correlation (r = -0.66, p = 0.003) with IMF content in Laiwu pig. Multiple regression analysis showed that CPT1B, FASN, solute carrier family 27 member 1 (SLC27A1), and fatty acid binding protein 3 (FABP3) contributed 38% of the prediction value for IMF content in Laiwu pigs. Of these four variables, CPT1B had the greatest contribution to IMF content (14%) followed by FASN (11%), SLC27A1 (9%), and FABP3 (4%). Conclusion: Our results indicate that the combined effects of an upregulation in fat deposition genes and downregulation in fat removal genes promotes IMF deposition in Laiwu pigs.

• 식물병연구
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• 제20권2호
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• pp.71-78
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• 2014

감마선과 융복합 처리를 위한 은나노 입자(NA, NSS), 이염화이소시안산나트륨(NaDCC) 등의 화학대체제를 이용하여 백합잎마름병의 원인균인 Botrytis elliptica의 포자발아 및 균사생장 억제와 동시에 백합 잎 절편에서 발병 과정 중 방제를 연구하였다. 발병과정에서 감마선 융복합 처리효과를 알아보기 위한 실험은 곰팡이 침입을 인위적으로 도와주는 상처접종과 병원균 스스로 침입을 하는 분무접종 두 가지를 실시하였다. 병원균 곰팡이 포자 발아를 90%까지 억제시킬 수 있는 감마선 선량인 $D_{10}$은 526 Gy였다. 2000 Gy까지 감마선 선량을 병원균 균사에 조사한 후, 16, 19, 22, 25, $28^{\circ}C$에서 배양한 결과 곰팡이 균사는 가장 높았던 2000 Gy에서도 죽지 않고 생장이 약 1-2일 지연되었을 뿐이었다. 백합 잎마름병을 억제하기 위해 필요한 1-2 kGy의 감마선 처리는 절화백합에서 부작용이 보일만큼 너무 높기 때문에 감마선은 200 Gy로 조사하고 다른 화학대체제들과의 융복합 처리하는 방안을 강구하였다. 다양한 화학대체제들의 융복합 처리 농도를 $40{\mu}g/l$로 맞춘 이유는 화학제만으로 병원균을 완전히 죽이지 않으면서 감마선의 살균 효과를 거둘 수 있기 때문이었다. 병원균 포자 발아 융복합 실험에서 $40{\mu}g/l$의 NaDCC 처리는 무처리에 비해 포자발아를 1000배 이하로 줄일 수 있었다. 감마선만 처리한 실험에서 방제가는 상처접종에서 23%, 분무접종에서 19.5%로 기대만큼 높지 않았다. 상처접종에서 감마선 처리하지 않은 NaDCC 단독처리의 방제가는 89%였던 반면, NaDCC와 200 Gy 융복합 처리는 32%에 불과하였다. 자연상태에 더 가까운 분무접종 실험에서 NaDCC 단독처리의 방제가는 50%, NaDCC와 200 Gy의 감마선 융복합 처리 방제가는 24%였다.

• 한국환경농학회지
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• 제34권1호
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• pp.46-51
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• 2015

본 연구는 친환경 유기농 살균제로 사용되고 있는 유황자재의 인삼탄저병 방제에 대한 효과와 약해가 발생하지 않는 적정 함량을 조사하는데 목적을 두고 있다. Colletotrichum gloeosporioides균에 의해서 발생하는 탄저병은 인삼의 수확량을 급격하게 감소시키는 파괴적인 병이다. 0.06% 황토유황합제와 0.13% 발효황토유황합제를 2년근 인삼에 처리를 했을 때 어떤 약해현상을 찾아 볼 수 없었다. 친환경자재의 치료효과를 알아보기 위해 탄저균을 접종 후에 다양한 친환경자재들을 처리했다. 석회유황합제(400배액)이 가장 높은 방제효과를 보였고 그다음은 발효황토유황합제(20배액), 발효황토유황합제(40배액), 석회유황합제(400배액) 순으로 높았다. 반면에 예방효과를 측정하기 위해서 병원균 접종 전 친환경자재들을 먼저 처리를 했다. 이 경우에는 발효황토유황합제(40배액)이 가장 높은 방제를 보였고 또한 대조구로 사용했던 기존농약과 비슷한 방제효과를 보였다. 그러므로 발효황토유황합제는 인삼탄저병을 방제하는 친환경 살균제로 사용할 수 있을 것으로 생각 된다.