• Asian Pacific Journal of Cancer Prevention
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• v.17 no.7
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• pp.3627-3629
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• 2016

Background: Activation and inactivation of nuclear factor of kappa light chain gene enhancer in B cells (NFKB) is tightly regulated to ensure effective onset and cessation of defensive inflammatory signaling. However, mutations within NFKB, or change in activation and inactivation molecules have been reported in a few cancers. Although oral squamous cell carcinoma is one of the most prevalent forms of cancer in India, with a development associated with malignant transformation of precancerous lesions, the genetic status of NFKB and relative rates of change in oral precancerous lesions remain unknown. Hence in the present study we investigated all twenty four exons of NFKB gene in two precancerous lesions, namely oral submucous fibrosis (OSMF) and oral leukoplakia (OL) to understand its occurrence, incidence and assess its possible contribution to malignant transformation. Materials and Methods: Chromosomal DNA isolated from twenty five each of OSMF and OL tissue biopsy samples were subjected to PCR amplification with intronic primers flanking twenty four exons of the NFKB gene. The PCR amplicons were subsequently subjected to direct sequencing to elucidate the mutation status. Results: Sequence analysis identified a novel heterozygous mutation, c.419T>A causing substitution of leucine with glutamine at codon 140 (L140Q) in an OL sample. Conclusions: The identification of a substitution mutation L140Q within the DNA binding domain of NFKB in OL suggests that NFKB mutation may be relatively an early event during transformation. To the best of our knowledge, this study is the first to have identified a missense mutation in NFKB in OL.

• Journal of Ginseng Research
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• v.27 no.1
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• pp.37-42
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• 2003

For study about introduce of gene connected with disease and transformation system of gingseng, chitinase gene cloned from soybene and disease resistant gene were carried out for expression and transformation of plant using Agrobacterium. The disease resistance gene(DR-49), 35S-35S-AMV, has been constructed. The disease resistance gene and chitinase gene were introduced into the binary vector pRD 400, which were mobilized into Agrobacterium tumefaciens faciens strain MP 90 and LBA 4404 harboring disarmed Ti-plasmid. As a result of induce transformants using ginseng embryo and petiole, multi shoots were formed on MS medium supplemented 1 mg/ι 2,4-D and 0.5 mg/ι kinetin. Also transformation by cotyledonwas effective on MS medium supplemented 1 mg/ι 2,4-D and 0.5 mg/ι kinetin, transformation percent of disease resistant gene and chitinase gene were showed 18%, 14% respectively. As transformed tissue is under pre-embryoid condition, normal shoot is required through the process of matured embryo.

• Journal of Distribution Science
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• v.16 no.2
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• pp.89-99
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• 2018

Purpose - In this study, we discuss the current status and barriers of digital transformation focused on small businesses. More specifically, this study consists of two studies. The purpose of study 1 is to investigate the effect of digitization of small businesses on the sales of stores and the number of visiting customers. The purpose of Study 2 is to examine the status and obstacles of online channels use by small businesses. Research design, data, and methodology - In Study 1, we will examine the changes in sales and visitor numbers of stores that are rapidly adapting to digital transformation among small business. For this, we utilize the actual situation of store management survey conducted by the Small Enterprise and Market Service. Specifically, multiple regression equations were used to determine whether blogs and online shopping malls were operating, and the proportion of credit cards to sales as independent variables and sales and number of visiting customers as dependent variables. Next, in Study 2, we surveyed the 15 small business owners in the latest survey on the actual situation of store management survey conducted in 2015, and conducted an in-depth interview to examine the barriers to the use of online channels by small business. Results - As a result of study 1, it is found that the small business who run the blogs have higher sales and visits than the small business who do not. However, there is no difference in the sales and the number of visiting customers between the stores that operate the online shopping malls and those that do not. Second, the higher the proportion of credit cards, the higher the sales and the number of visiting customers. In study 2, we analyzed the barriers to the expansion of online channels by in-depth interviews. Interviews show that barriers to access to online channels are limited by search neutrality, high commission burden, and low bargaining power. These problems are caused by the insufficiency of small business compared to online and mobile portal and O2O platform vendors. Conclusions - This study suggests that small business who have difficulty in establishing direct online channels need digital transformation of small business. In addition, when using such an external platform, we have identified the problems that small business face.

• Journal of the Korean Society of Industry Convergence
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• v.23 no.5
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• pp.717-726
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• 2020

Social costs are increasing in Gangwon-do east coast due to coastal erosion. Long-term coastline change information is essential for analyzing this phenomenon. In this study, aerial photographs immediately after liberation are used for 1950's coastline extraction. The study area is from Sokcho Cheongho beach to Yangyang Seorak beach. The aerial photograph is geometrically corrected using DLT(Direct Linear Transformation) method to extract past coastline and compare it with present data. Coastal erosion and deposition areas are calculated in study area. Artificial structures such as harbors and breakwaters have caused changes in ocean currents and sediments from river estuaries. In most cases, the deposition occurred at the southern area of artificial structures and the erosion occurred on surrounding beaches. Coastline information extracted from past aerial photographs can be useful to provide information on long-term changes.

• Proceedings of the KIEE Conference
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• 2002.07a
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• pp.108-110
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• 2002

In this paper, a fault location algorithm is suggested for line to line faults in distribution networks. Conventional fault location algorithms use the symmetrical component transformation, a very useful tool for transmission network analysis. However, its application is restricted to balanced network only. Distribution networks are, in general, operated in unbalanced manners, therefore, conventional methods cannot be applied directly, which is the reason why there are few research results on fault location in distribution networks. Especially, the line to line fault is considered as a more difficult subject. The proposed algorithm uses direct 3-phase circuit analysis, which means it can be applied not only to balanced networks but also to unbalanced networks like distribution a network. The comparisons of simulation results between one of conventional methods and the suggested method are presented to show its effectiveness and accuracy.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.29 no.3 s.234
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• pp.369-386
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• 2005

Various aspects of structural optimization techniques under transient loads are extensively reviewed. The main themes of the paper are treatment of time dependent constraints, calculation of design sensitivity, and approximation. Each subject is reviewed with the corresponding papers that have been published since 1970s. The treatment of time dependent constraints in both the direct method and the transformation method is discussed. Two ways of calculating design sensitivity of a structure under transient loads are discussed - direct differentiation method and adjoint variable method. The approximation concept mainly focuses on re- sponse surface method in crashworthiness and local approximation with the intermediate variable Especially, as an approximated optimization technique, Equivalent Static Load method which takes advantage of the well-established static response optimization technique is introduced. And as an application area of dynamic response optimization technique, the structural optimization in flexible multibody dynamic systems is re- viewed in the viewpoint of the above three themes

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.1
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• pp.189-205
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• 1996

The twin-roll type strip continuous casting process(or direct rolling process) of steel materials is characterized by two rotating water cooled rolls receiving a steady supply of molten metal which solidifies onto the rolls. A solidification analysis of molten metal considering phase transformation and thermofluid is performed using finite diffefence method with curvilinear coordinate to reduce computing time and molten region analysis with arbitrary shape. An enthalpy-specific heat method is used to determine the temperatures inthe roll and the steel. The temperature distribution of cooling roll is calculated using two dimensional finite element method, because of complex roll shape due to cooling hole in rolls and improvemnt accuracy of calculation result. The energy equaiton of cooling roll is solved simultanuously with the conservation equaiton of molten metal in order to consider heat transfer through the cooling roll. The calculated roll temperature is compared to experimental results and the heat transfer coefficient between cooling roll surface and rolling material(steel) is also determined from comparison of measured roll temperature and calculated temperature.

• Journal of Korean Society for Geospatial Information Science
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• v.12 no.2 s.29
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• pp.67-71
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• 2004

Increasing number and acquisition rate of satellite imagery promoted researches related with DEM generation based on satellite imagery. SPOT image gave us advantage to generate DEM which covers wide area of $60km{\times}60km$. In the case of rigorous sensor model of SPOT imagery, ephemeris data and several ground control points are need and requires arduous computational costs to produce DEM. In this study, using alternative sensor model based on Direct Linear Transform, we generated DEM using small number of ground control points. As a result, it was possible to acquire the DEM with suitable accuracy.

• Journal of Plant Biotechnology
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• v.36 no.2
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• pp.157-162
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• 2009

High frequency plant proliferation system via direct frond regeneration of endemic duckweed plants Lemna paucicostata and Spirodela polyrhiza was established. Fronds of L. paucicostata and S. polyrhiza were able to multiply half-strength MS basal medium without plant growth regulators. However, addition of BA at a range of 1 to 3 mg/L was more effective than high concentration of BA treatments for fronds proliferation. Also half-strength MS salts was suitable for the fronds proliferation. Increase of salts concentration had inhibitory effect on fronds proliferation. Also the frequency of callus formation from fronds of L. paucicostata was 3.3%, when they cultured onto 1/2 MS medium supplemented with 1 mg/L of BA. Similarly the frequency of callus formation from S. polyrhiza was very low. After subculture of white globular structures derived from fronds of L. paucicostata, numerous globular somatic embryos and calluses were developed onto the surface of fronds. However these somatic embryos did not fully develop into normal plants when transferred to 1/2 MS basal medium. Therefore direct frond regeneration system was more efficient for mass proliferation of L. paucicostata and S. polyrhiza. The plant regeneration system of L. paucicostata and S. polyrhiza established in this study, might be applied to mass proliferation and genetic transformation for molecular breeding.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.44 no.3
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• pp.98-107
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• 2007

For high-dimensional pattern recognition, such as face classification, the small number of training samples leads to the Small Sample Size problem when the number of pattern samples is smaller than the number of dimensionality. Recently, various LDA-extensions have been developed, including LDA, PCA+LDA, and Direct-LDA, to address the problem. This paper proposes a method of improving the classification efficiency by increasing the number of (sub)-classes through pre-clustering a training set prior to the execution of Direct-LDA. In LDA (or Direct-LDA), since the number of classes of the training set puts a limit to the dimensionality to be reduced, it is increased to the number of sub-classes that is obtained through clustering so that the classification performance of LDA-extensions can be improved. In other words, the eigen space of the training set consists of the range space and the null space, and the dimensionality of the range space increases as the number of classes increases. Therefore, when constructing the transformation matrix, through minimizing the null space, the loss of discriminatve information resulted from this space can be minimized. Experimental results for the artificial data of X-OR samples as well as the bench mark face databases of AT&T and Yale demonstrate that the classification efficiency of the proposed method could be improved.