Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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High frequency plant proliferation via direct fronds regeneration of Korean endemic duckweed species

국내 자생 좀개구리밥 (Lemna paucicostata) 및 개구리밥(Spirodela polyrhiza)의 엽상체 증식을 통한 기내 식물체 대량 증식체계 확립

  • 오명진 (한국생명공학연구원 생물자원센터) ;
  • 박종미 (한국생명공학연구원 생물자원센터) ;
  • 고석민 (유진텍(주) 부설연구소) ;
  • 유장렬 (식물시스템연구센터) ;
  • 김석원 (한국생명공학연구원 생물자원센터)
  • Published : 2009.06.30
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Abstract

High frequency plant proliferation system via direct frond regeneration of endemic duckweed plants Lemna paucicostata and Spirodela polyrhiza was established. Fronds of L. paucicostata and S. polyrhiza were able to multiply half-strength MS basal medium without plant growth regulators. However, addition of BA at a range of 1 to 3 mg/L was more effective than high concentration of BA treatments for fronds proliferation. Also half-strength MS salts was suitable for the fronds proliferation. Increase of salts concentration had inhibitory effect on fronds proliferation. Also the frequency of callus formation from fronds of L. paucicostata was 3.3%, when they cultured onto 1/2 MS medium supplemented with 1 mg/L of BA. Similarly the frequency of callus formation from S. polyrhiza was very low. After subculture of white globular structures derived from fronds of L. paucicostata, numerous globular somatic embryos and calluses were developed onto the surface of fronds. However these somatic embryos did not fully develop into normal plants when transferred to 1/2 MS basal medium. Therefore direct frond regeneration system was more efficient for mass proliferation of L. paucicostata and S. polyrhiza. The plant regeneration system of L. paucicostata and S. polyrhiza established in this study, might be applied to mass proliferation and genetic transformation for molecular breeding.

Keywords

References

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