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http://dx.doi.org/10.5142/JGR.2003.27.1.037

Vector Construction and Transformation of Ginseng (Panax ginseng C.A. Meyer) Using Disease Resistant Genes  

Yang, Deok-Chun (College of Life Science, Kyung Hee University)
Lee, Eun-Kyung (College of Life Science, Kyung Hee University)
Kim, Moo-Sung (College of Life Science, Kyung Hee University)
Publication Information
Journal of Ginseng Research / v.27, no.1, 2003 , pp. 37-42 More about this Journal
Abstract
For study about introduce of gene connected with disease and transformation system of gingseng, chitinase gene cloned from soybene and disease resistant gene were carried out for expression and transformation of plant using Agrobacterium. The disease resistance gene(DR-49), 35S-35S-AMV, has been constructed. The disease resistance gene and chitinase gene were introduced into the binary vector pRD 400, which were mobilized into Agrobacterium tumefaciens faciens strain MP 90 and LBA 4404 harboring disarmed Ti-plasmid. As a result of induce transformants using ginseng embryo and petiole, multi shoots were formed on MS medium supplemented 1 mg/ι 2,4-D and 0.5 mg/ι kinetin. Also transformation by cotyledonwas effective on MS medium supplemented 1 mg/ι 2,4-D and 0.5 mg/ι kinetin, transformation percent of disease resistant gene and chitinase gene were showed 18%, 14% respectively. As transformed tissue is under pre-embryoid condition, normal shoot is required through the process of matured embryo.
Keywords
Chitinase gene; cotyledon; diease resistance; embryo; petiole;
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