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Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease

  • SHIN Myeong Heon;CHUNG Young-Bae;KITA Hirohito
    • Parasites, Hosts and Diseases
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    • v.43 no.1 s.133
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    • pp.33-37
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    • 2005
  • Eosinophil degranulation is considered to be a key effector function for the killing of helminthic worms and tissue inflammation at worm-infected lesion sites. However, relatively little data are available with regard to eosinophil response after stimulation with worm-secreted products which contain a large quantity of cysteine proteases. In this study, we attempted to determine whether the degranulation of human eosinophils could be induced by the direct stimulation of the excretory-secretory products (ESP) of Paragonimus westermani, which causes pulmonary paragonimiasis in human beings. Incubation of eosinophils for 3 hr with Paragonimus-secreted products resulted in marked degranulation, as evidenced by the release of eosinophil-derived neurotoxin (EON) in the culture supernatants. Moreover, superoxide anion was produced by eosinophils after stimulation of the ESP. The ESP-induced EDN release was found to be significantly inhibited when the ESP was pretreated with protease inhibitor cocktail or the cysteine protease inhibitor, E-64. These findings suggest that human eosinophils become degranulated in response to P. westermani-secreted proteases, which may contribute to in vivo tissue inflammation around the worms.

A Study on the Store Choice Criteria and Store-Related Attitudes of Consumers in accordance with the Clothing Shopping Motives and Involvement of Customers (소비자의 의복 쇼핑동기와 관여도에 따른 점포 선택기준과 점포에 대한 태도)

  • Hong, Keum-Hee;Kang, Hye-Lie
    • Fashion & Textile Research Journal
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    • v.5 no.4
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    • pp.363-371
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    • 2003
  • This study is designed to identify the dimensions of clothing shopping motives and clothing involvements which influence consumers' store choice behaviors and to find out what variables have a direct influence on the attitudes of customers toward stores. The empirical research was made through the survey on 300 female and male shoppers who purchased clothing. The major results of this study are as follows : 1. The dimension of clothing shopping motives is composed of leisure-related shopping motive, products-related shopping motive, and economy-related shopping motive. And the dimension of clothing involvement consists of pleasure involvement factor, fashion involvement factor, and symbol involvement factor. 2. The dimension of the store choice criteria is composed of such five factors as promotion, products, prices/salespersons, service, and location. 3. There is a positive correlation of the products-related shopping factor and the leisure-related shopping motive only. And the store choice criteria have a positive correlation with all the factors of clothing involvement. 4. The high-shopping-motive group turns out to pay more importance to such factors as products and location than the low-shopping-motive group. 5. The variables which have influences on the attitudes of customers toward stores tum out to be such factors as fashion involvement, promotion in the store choice criteria, and gender.

A Study on the Reasonable Materials Handling System of Wooden Furniture Industries (목재가구산업의 적정 물류운반시스템에 관한 연구)

  • Chung, Woo-Yang
    • Journal of the Korean Wood Science and Technology
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    • v.24 no.2
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    • pp.71-80
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    • 1996
  • Handling the manufacured goods is the most cost-consuming task in material handling system in wooden furniture industries. Fully automated warehouse for cartoned goods of a furniture manufacturing company was analized to provide plant engineers and managers with some important informations on the utility and profitability of the automated storage and retrieval system(AS/RS). Process-oriented simulation modeling tech-niques were used to describe the system and to propose some alternatives to promote the efficiency of AS/RS. Simulation report could be inter-preted as follows: 1. Warehouse for the furniture goods must be designed in accordance with reliable material handling program and constructed with suitable equipments depending on the specification of packed products. 2. An excess of palletized products induced the indigestion and the inefficiency of AS/RS of the furniture industry and put this high-costly system into the shade. 3. Overcrowded AS/RS of the furniture factories could regain its material handling function by cutting down the deposit of products into the automated warehouse. For this purpose, reducing the regular output and direct delivering the outside products to the destination should be considered as the definite counterplan. And additional operation of conventional handy warehouse was also expected to improve the efficiency of main AS/RS.

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A Study on CAD System for Shapes Segmentation & Rendering Simulation (CAD SYSTEM을 활용한 디자인 다각화 방향과 시뮬레이션 연구 - 사무용 회전의자를 사례로 한 개발 제품의 모형화 접근 -)

  • Seo Dong-Jin
    • Journal of the Korea Furniture Society
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    • v.14 no.1
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    • pp.35-45
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    • 2003
  • It has shown that the investment on the new products have a direct influence on the price of manufactured goods. This brings about a point, which has been a common problem for many Korean enterprises. That is Korean products can only its international competitiveness from the price, not quality. Our industry has shown its movement from producing cheap, low quality products to creating somewhat more advanced and higher qualify products. However the industry cannot avoid the financial burden from the investment. Just like the title of this article, for existing the 3D-MODELING, the 3D-CAD is existed first has immediate effect on the many developing processes, such as planning a product, designing, and producing. The development of the furniture design in the future will be done in the same way as this article presents. This will have a great effect on the overall productivity. (Time, cost, efficiency and etc). The simulation experiment on this article is based on the idea of development of industry, forms the immediate constituent for the prediction related to the production development, marketing and the trend. Which means, this will play an important part where companies achieve satisfaction from the marketing part and the economy.

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Quantitation of Recombinant Hirudin by Enzyme-Linked Immunosorbent Assay (효소면역측정법 (ELISA)을 이용한 유전자 재조합 히루딘의 정량)

  • Choi, Yun-Joo;Hahn, Bum-Soo;Ahn, Mi-Young;Park, Pyung-Keun;Sohn, Jung-Hoon;Choi, Eui-Sung;Lee, Sang-Ki;Kim, Yeong-Shik
    • YAKHAK HOEJI
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    • v.41 no.1
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    • pp.74-80
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    • 1997
  • A polygonal antibody against recombinant hirudin was raised for the development of a ELISA in biological fluids. Recombinant hirudin was conjugated to maleimide activated carrie r protein, KLH and injected to a rabbit. The third booster collection of antiserum was used as primary antibody for the ELISA. The titer for the detection antibody was determined. The direct ELISA could determine the concentration of hirudin in the range of ~10ng/ml. Affinity pulified IgG was obtained and conjugated to horseradish peroxidase. Purified IgG and IgG-HRP could be used as capture and detection antibody, respectively. Although sandwich ELISA would not give the satisfactory results. it could apply for the detection of hirudin level in the range of ~20 ${\mu}$g/ml.

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A New Functional Model Complex of Extradiol-cleaving Catechol Dioxygenases: Properties and Reactivity of [$Fe^{II}$(BLPA)DBCH]BPh₄

  • Lim, Ji H.;Park, Tae H.;이호진;이강봉;Jang, Ho G.
    • Bulletin of the Korean Chemical Society
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    • v.20 no.12
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    • pp.1428-1432
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    • 1999
  • [Fe$^{II}$(BLPA)DBCH]BPh₄ (1), a new functional model for the extradiol-cleaving catechol dioxygenases, has been synthesized, where BLPA is bis(6-methyl-2-pyridylmethyl)(2-pyridylmethyl)amine and DBCH is 3,5-di-tert-butylcatecholate monoanion. ¹H NMR and EPR studies confirm that 1 has a high-spin Fe(II) (S = 2) center. The electronic spectrum of 1 exhibits one absorption band at 386 nm, showing the yellow color of the typical [Fe$^{II}$(BLPA)] complex. Upon exposure to O₂, 1 is converted to an intense blue species within a minute. This blue species exhibits two intense bands at 586 and 960 nm and EPR signals at g = 5.5 and 8.0 corresponding to the high-spin Fe(III) complex (S = 5/2, E/D = 0.11). This blue complex further reacts with O₂ to be converted to (μ-oxo)Fe$^{III}_2$ complex within a few hours. Interestingly, 1 affords intradiol cleavage (65%) and extradiol cleavage (20%) products after the oxygenation. It can be suggested that 1 undergoes two different oxygenation pathways. The one takes the substrate activation mechanism proposed for the intradiol cleavage products after the oxidation of the $Fe^II\;to\;Fe^{III}$. The other involves the direct attack of O₂ to $Fe^{II}$ center, forming the $Fe^{III}$-superoxo intermediate which can give rise to the extradiol cleavage products. 1 is the first functional Fe(II) complex for extradiol-cleaving dioxygenases giving extradiol cleavage products.

The Urgency of Business Agility During COVID-19 Pandemic: Distribution of Small and Medium Business Products and Services

  • BONGSO, Gromyko;HARTOYO, Rachmat
    • Journal of Distribution Science
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    • v.20 no.6
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    • pp.57-66
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    • 2022
  • Purpose: Business agility is an important key to survival for SMEs in Indonesia, especially during the COVID-19 pandemic. Indonesian local product distribution and service distribution are mostly served by SMEs. Agile businesses will be able to assist them in the proper distribution of products and services. This research examines how the direct and indirect influence of IT capabilities on business agility through organizational learning and business intelligence for small and medium enterprises in the distribution of Indonesian products and services. Research design, data and methodology: This research uses SEM method with SmartPLS tool. The sample of this research was conducted on small and medium enterprises in the distribution of Indonesian products and services. The sample obtained in this study was 202 SME owners or managers (strategic level). Results: Business intelligence plays a key role in improving business agility. The results of IT capability can directly and indirectly affect business agility through organizational learning. Conclusions: Business intelligence has the biggest role in increasing business agility in SMEs in Indonesia. IT capability has an indirect effect on business agility through organizational learning. The findings of this study prove that IT capabilities do not indirectly affect business agility through business intelligence.

The Effects of Consumer Innovation, Price Sensitivity, Conformity, and Consumer Values on Consumer Attitudes and Intentions to Participate in the Crowdfunding of Fashion Products (소비자 혁신성, 가격민감도, 동조 성향, 소비자 가치가 패션제품의 크라우드펀딩에 대한 소비자 태도 및 펀딩참여의도에 미치는 영향)

  • Jae Hee Kim;Nalae Kim;Yoon-Jung Lee
    • Human Ecology Research
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    • v.61 no.3
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    • pp.281-295
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    • 2023
  • This study examined consumer attitudes and intentions to participate in reward-based crowdfunding projects for fashion products. We focused on consumer innovation, price sensitivity, conformity, and consumer values as factors that might influence such attitudes and intentions. A survey was conducted with 228 individuals aged 18 years and older who are aware of or have had experience using crowdfunding. Respondents were asked to answer questions based on the example of actual fashion crowdfunding projects. To analyze the data, reliability tests, the generation of descriptive statistics, factor analysis, and multiple regression were performed using SPSS 25.0. The results revealed that consumer innovativeness, non-conforming tendency, and emotional, quality, social, and ethnical consumer values had a significant influence on attitudes, which in turn affected intentions to participate in crowdfunding. Consumer innovativeness, price sensitivity, emotional value, and social value also had a direct influence on intentions to participate. In addition to its academic contribution, this study has important marketing implications for project initiators striving to identify and understand consumers who are willing to participate in reward-based crowdfunding for fashion products.

Analysis on the Characteyistics of Consumer′s Consumption Types of Environmently Friendly Agricultural Products (친환경 농산물 소비자의 소비행태에 관한 특성분석)

  • Bae Sung Eui;Yoon Jun Sang;Lee Jong Sang;Kim Chang Ho;Yoon Gil Sun
    • The Korean Journal of Community Living Science
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    • v.15 no.4
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    • pp.149-163
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    • 2004
  • The purpose of this study is to present a plan to activate the production and consumption of environmently friendly agricultural products through an analysis on the understanding degree, the level and inclination of consumption, the satisfaction degree of consumption, and the comprehension degree of production and circulation process of the environmently friendly agricultural products. 1 reviewed the literature and did survey In this study. The results are as follows : (1) As a result of an analysis on the understanding degree of the environmently friendly agricultural products, it is shown to get 6.37 points out of 10 and also, there is a difference between age, educational background, and income level. (2) In the case of research on the method to distinguish the environmently friendly agricultural products from others, it is proved that consumers have a strong faith on quality guarantee marks and there is a difference between gender, income level, and consumption period. (3) It is also shown that consumers purchase environmently friendly agricultural products for the family member's health in the survey on the motivation for purchasing them. And also, there is a difference between men and women. (4) The result of the survey on the consumption scale of the environmently friendly agricultural products shows that 94 people(60.3%) spend more than 50% of their gloss foodstuffs cost purchasing them and 20 people(12.8%) spend 30-50%. (5) In the survey on the amount and the frequency of the purchasing items of the envirounently friendly agricultural products, respondents count livestock products mostly in amount while point out main cereals and a kinds of soy and pastes in frequency. (6) Consumers ask that the price of environmently friendly agricultural products is a little expensive or reasonable and there is no differences between individual groups. (7) In the case of the purchase of foreign environmently friendly agricultural products, there are more people who want to purchase domestic ones than those who want foreign ones. Therefore, it is shown that environmently friendly agricultural products have a counterpower after the market-open to import. (8) As a result of the analysis on the quality of environmently friendly agricultural products, it is proved that they have better quality than general agricultural products and also, it is found out that women feel larger differences than men in quality. (9) In the analysis on the satisfaction degree of the environmently friendly agricultural products, it is proved that respondents trust the safety and nutrition of them whereas they are not satisfied with the external shape of them. (10) It is analyzed that tile conversion of consumer's consciousness is the most critical factor for development of environmently friendly agriculture. (11) The factors to activate the consumption of environmently friendly agricultural products are proved in order of the enlargement of direct transaction, the conversion of consumer's consciousness, the easiness of purchase, activating consumers'unions, and publicity.

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Effects of K-drama on attitudes of Chinese consumers toward Korean fashion products - The role of perceived similarity and people image - (중국 소비자들의 한국 TV드라마 시청이 한국 패션제품 태도 형성에 미치는 영향 - 드라마 등장인물과의 유사성과 국민이미지 역할을 중심으로 -)

  • Park, Jee-Sun;Jeong, So Won;Lee, Kyu-Hye
    • The Research Journal of the Costume Culture
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    • v.25 no.1
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    • pp.32-47
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    • 2017
  • As the popularity of Korean drama and celebrities in China, Korean fashion is becoming increasingly popular in the Chinese market. Although the effect of Korean drama on Chinse consumers' attitudes toward Korean products are known, little research has been conducted to understand the mechanisms underlying the impact of Korean drama on the development of consumer attitudes. Thus, this study examines how Chinese consumers' exposure to Korean dramas has influenced their attitudes towards Korean fashion products. Applying the similarity-attraction theory, the study explores the roles Chinese consumers' perceived similarities in appearance and values with Korean characters in TV dramas plays in the process of attitude development. Data was collected via an online survey and the responses of 317 Chinese consumers in their twenties were used for data analysis. The results of structural equation modeling show that exposure to Korean dramas has a direct impact on Chinese consumers' perceived appearance similarity, perceived value similarity, image of Korean people, and attitudes toward Korean fashion products-results that support the theory of mere exposure. In addition, the analysis demonstrates that perceived appearance similarity positively influences the image of Koreans among Chinese people, which, in turn, influences attitudes toward Korean fashion products, supporting the similarity-attraction theory. However, the effect of perceived value similarity on attitude toward Korean fashion products was not significant. The study concludes by describing its practical implications for the Korean fashion industry and presenting ideas for future research.