• 동의생리병리학회지
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• 제23권3호
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• pp.613-618
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• 2009

Although the effect of Eucommie umoides oliver in osteoporosis has been studied, direct action of Eucommis ulmoides Oliver on osteoclasts remains unknown. Here we examined whether Eucommiae cortex inhibits osteoclast differentiation and bone resorption, a process known to be involved in bone diseases such as osteoporosis. Water extract from Eucommiae cortex (WE-EC) inhibited differentiation of bone marrow macrophages (BMMs) into osteoclasts without causing cytotoxicity. WE-EC suppressed the phosphorylation of p38, ERK, and JNK in BMMs treated with RANKL. WE EC specifically suppressed the mRNA expression of NFATc1 induced by RANKL. However, WE-EC inhibited stability of c-Fos protein induced by RANKL. Furthermore, WE-EC inhibited osteoclast survival induced by RANKL and in turn suppressed bone resorption. Taken together, our results suggest that WE-EC may be better agents for therapeutic use in bone diseases.

• Plant Biotechnology Reports
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• 제3권4호
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• pp.333-340
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• 2009

Developmental anomalies in the plumule meristem of peanut (Arachis hypogaea L.) somatic embryos resulted in poor shoot differentiation and reduced plant recovery. Existing meristems with caulogenic potential have never been tested for embryogenesis in peanut. The present experiment was designed to test the mature zygotic embryo axis derived plumule with three meristems for somatic embryogenesis. Embryogenic masses and embryos developed from the caulogenic meristems in the axils. Exposure of 2 weeks in primary medium with $90.5{\mu}M$ 2,4-D suppressed the shoot tip differentiation temporarily which then regained the ability to form the shoot on withdrawal of 2,4-D. Exposure of 4 weeks in primary medium with $90.5{\mu}M$ 2,4-D suppressed the shoot tip differentiation irreversibly. No shoot formation was noted from the tips in any of the cultures which were in secondary medium with $13.6{\mu}M$ 2,4-D. Development of somatic embryos directly from axillary meristems was confirmed histologically. Conversion frequency of these embryos was 11%. Thus, in this report, we describe a method to obtain somatic embryos from the determined organogenic buds of the axillary meristem, by culturing the nodal explant vertically on embryo induction medium. It also displays the possibility of obtaining both embryogenic and organogenic potential in two parts of the same explant simultaneously. The possibility of extending this approach for genetic transformation in in vivo system through direct DNA delivery or Agrobacterium injection in meristems can also be explored. Using Agrobacterium rhizogenes, we have demonstrated the possibility of gene transfer in the axillary meristems of seed-derived plumule explant.

• 치위생과학회지
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• 제21권4호
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• pp.243-250
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• 2021

Background: Endodontic sealers or their toxic components may become inflamed and lead to delayed wound healing when in direct contact with periapical tissues over an extended period. Moreover, an overfilled sealer can directly interact with adjacent tissues and may cause immediate necrosis or further resorption. Therefore, the treatment outcome conceivably depends on the endodontic sealer's biocompatibility and osteogenic potential. This study aimed to evaluate the cell viability and osteogenic effects of four different sealers in osteoblastic cells. Methods: AH Plus (resin-based sealer), Pulp Canal Sealer EWT (zinc oxide-eugenol sealer), BioRoot RCS (calcium silicate-based sealer), and Well-Root ST (MTA-based calcium silicate sealer) were mixed strictly according to the manufacturer's instructions, and dilutions of sealer extracts (1/2, 1/5 and 1/10) were determined. Cell viability was measured using the water-soluble tetrazolium-8 (WST-8) assay. Differentiation was assessed by alkaline phosphatase (ALP) activity and mineralized nodule formation by Alizarin Red S staining. Results: The cell viability of the extracts derived from the sealers excluding Well-Root ST was concentration dependent, with sealer extracts having the least viability at a 1/2 dilution. At sealer extract dilution of 1/10, the test groups showed the same survival rate as that control group, with the exception of BioRoot RCS. Among all experimental groups, BioRoot RCS showed the highest cell viability after 48 hours. The ALP activity was significantly higher in a concentration-dependent manner. Furthemore, all four materials promoted ALP activity and mineralized nodule formation compared to the control at 1/10 dilutions. Conclusion: This is the first study to highlight the differences in biological activity of these four materials. These results suggest that the composition of root canal sealers appears to alter the form of biocompatibility and osteoblastic differentiation.

• 치위생과학회지
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• 제23권2호
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• pp.169-175
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• 2023

Background: Although microbial infection is direct cause of periodontal disease, various environmental factors influence the disease severity. Aging is considered a risk factor for oral diseases, with the prevalence of periodontal diseases increasing with age. Moreover, senescence-associated secretory phenotype (SASP) expressed in age-related diseases is a key marker of chronic inflammation and aging phenotypes. Therefore, this study aimed to understand the relevance of senescent cells to periodontal health and disease, investigate the possibility of regulating the expression of aging- and osteolysis-related factors in gingival fibroblasts, and investigate the effect of senescence induction in gingival fibroblasts on osteoclast differentiation in mouse bone marrow-derived macrophages (BMMs). Methods: After stimulation with 400 nM hydrogen peroxidase, human gingival fibroblasts (HGFs) were examined for senescence-associated β-galactosidase. Western blot and enzyme-linked immunosorbent assays were performed to assess the expression of SASP. Osteoclast formation was assessed in BMMs using a conditioned medium (CM) from hydrogen peroxide-stimulated HGFs. Osteoclastic differentiation was investigated using tartrate-resistant acid phosphatase (TRAP) staining and activity. Data analysis was performed using SPSS version 25.0. Results: The expression of senescence-related molecules, including p53, p16, and p21, and the expression of osteolytic factors, including IL-6, IL-8, and IL-17, were found to be significantly higher in the hydrogen peroxide-stimulated HGF than in the control group. Regarding the indirect effects of senescent gingival cells, the number of osteoclasts and TRAP activity increased according to the differentiation of BMM cultured in CM. Conclusion: Our results on the of between osteolytic factors and cellular senescence in gingival fibroblast cells helped to reveal evidence of pathological aging mechanisms. Furthermore, our results suggest that the development of novel therapies that target specific SASP factors could be an effective treatment strategy for periodontal disease.

• International Journal of Stem Cells
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• 제16권1호
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• pp.93-107
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• 2023

Background and Objectives: Chronic periodontitis can lead to alveolar bone resorption and eventually tooth loss. Stem cells from exfoliated deciduous teeth (SHED) are appropriate bone regeneration seed cells. To track the survival, migration, and differentiation of the transplanted SHED, we used super paramagnetic iron oxide particles (SPIO) Molday ION Rhodamine-B (MIRB) to label and monitor the transplanted cells while repairing periodontal bone defects. Methods and Results: We determined an appropriate dose of MIRB for labeling SHED by examining the growth and osteogenic differentiation of labeled SHED. Finally, SHED was labeled with 25 ㎍ Fe/ml MIRB before being transplanted into rats. Magnetic resonance imaging was used to track SHED survival and migration in vivo due to a low-intensity signal artifact caused by MIRB. HE and immunohistochemical analyses revealed that both MIRB-labeled and unlabeled SHED could promote periodontal bone regeneration. The colocalization of hNUC and MIRB demonstrated that SHED transplanted into rats could survive in vivo. Furthermore, some MIRB-positive cells expressed the osteoblast and osteocyte markers OCN and DMP1, respectively. Enzyme-linked immunosorbent assay revealed that SHED could secrete protein factors, such as IGF-1, OCN, ALP, IL-4, VEGF, and bFGF, which promote bone regeneration. Immunofluorescence staining revealed that the transplanted SHED was surrounded by a large number of host-derived Runx2- and Col II-positive cells that played important roles in the bone healing process. Conclusions: SHED could promote periodontal bone regeneration in rats, and the survival of SHED could be tracked in vivo by labeling them with MIRB. SHED are likely to promote bone healing through both direct differentiation and paracrine mechanisms.

• 한국지반공학회논문집
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• 제25권9호
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• pp.93-100
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• 2009

본 연구에서 역해석에 이용한 직접법은 실제 변위와 목적함수의 차이를 최소화하고 미지변수를 보정하는 최적화가 핵심 과정이다. 연구과정이 복수의 지층을 단계별로 역해석하여 각 지반의 미지변수를 역해석하기 때문에 지층 간 미지변수의 간섭 효과를 최소화 할 수 있는 추가적인 최적화가 필요하다. 따라서 효율적이고 정확한 최적화를 위해서 목적함수의 편미분 계산과정이 없이 최적해를 구할 수 있는 직접탐색법(direct search method)을 사용하였다. 본 연구의 목적은 지하구조체가 포함된 지층을 상부와 하부의 지층으로 모형화하고, 다중 미지변수를 역해석하는 기법을 개발하는 데에 목적을 둔다. 또한 단계별 역해석된 미지변수의 상호 간섭 오차를 최소화하기 위해서 반복 역해석을 실시하고, 실제 존재하는 터널에 대하여 적용성에 대해 검토하였다. 그 결과, 단층 모형화보다 다층 모형화 결과가 실제 지하구조물거동을 정확히 나타냈으며 다층 경계부에 위치한 지하구조물의 다층지반 모형화를 통한 역해석이 유효함을 확인하였다.

• 대한기계학회논문집A
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• 제29권3호
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• pp.369-386
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• 2005

Various aspects of structural optimization techniques under transient loads are extensively reviewed. The main themes of the paper are treatment of time dependent constraints, calculation of design sensitivity, and approximation. Each subject is reviewed with the corresponding papers that have been published since 1970s. The treatment of time dependent constraints in both the direct method and the transformation method is discussed. Two ways of calculating design sensitivity of a structure under transient loads are discussed - direct differentiation method and adjoint variable method. The approximation concept mainly focuses on re- sponse surface method in crashworthiness and local approximation with the intermediate variable Especially, as an approximated optimization technique, Equivalent Static Load method which takes advantage of the well-established static response optimization technique is introduced. And as an application area of dynamic response optimization technique, the structural optimization in flexible multibody dynamic systems is re- viewed in the viewpoint of the above three themes

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.917-923
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• 2014

Chondrosarcomas are malignant cartilage-forming tumors of bone which exhibit resistance to both chemotherapy and radiation treatment. miRNAs have been well demonstrated to regulate gene expression and play essential roles in a variety of biological processes, including proliferation, differentiation, migration, cell cycling and apoptosis. In this study, we obtained evidence that miR-100 acts as a tumor suppressor in human chondrosarcomas. Interestingly, cisplatin resistant chondrosarcoma cells exhibit decreased expression of miR-100 compared with parental cells. In addition, we identified mTOR as a direct target of miR-100. Overexpression of miR-100 complementary pairs to the 3' untranslated region (UTR) of mTOR, resulted in sensitization of cisplatin resistant cells to cisplatin. Moreover, recovery of the mTOR pathway by overexpression of S6K desensitized the chondrosarcoma cells to cisplatin, suggesting the miR-100-mediated sensitization to cisplatin dependent on inhibition of mTOR. In summary, the present studies highlight miR-100 as a tumor suppressor in chondrosarcoma contributing to anti-chemoresistance. Overexpression of miR-100 might be exploited as a therapeutic strategy along with cisplatin-based combined chemotherapy for the treatment of clinical chondrosarcoma patients.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 학술대회 논문집 전문대학교육위원
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• pp.47-52
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• 2001

This paper presents a high-performance control system for Reluctance Synchronous Motor (RSM) drives with direct torque control (DTC). The system consist of stator flux observer, rotor position/speed estimator, torque estimator, two hysteresis band controllers, an optimal switching look-up table, IGBT voltage source inverter, and F240/C31DSP controller by using fully integrated control software. The stator flux observer is based on the combined voltage and current model with stator flux feedback adaptive control that inputs are current and voltage sensing of motor terminal with estimated rotor angle for wide speed range. The rotor position is estimated by the observed stator flux-linkage space vector. The estimated rotor speed can be determinated by differentiation of the rotor position used only in the current model part of the flux observer for a low speed operating area. To prove the suggested control algorithm, we have a simulation and testing at actual experimental system. The developed digitally high-performance position sensorless control system are shown a good motion control response characteristic results and high performance features using 1.0Kw RSM.

• Journal of Power Electronics
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• 제2권2호
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• pp.146-153
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• 2002

this paper presents an implementation of digital control system of speed sensorless for Reluctance Synchronous Motor (RSM) drives with direct torque control (DTC). The problem of DTC for high-dynamic performance RSM drive is generating a nonlinear torque due to a saturated nonlinear inductance curve with various load currents. The control system consists of stator flux observer, compensating inductance look-up table, rotor position/speed/torque estimator, two hysteresis band controllers, an optimal switching look-up table, IGBT voltage source unverter, and TMS320C31 DSP controller. The stator flux observer is based on the combined voltage and current model with stator flux feedback adapitve control that inputs are the compensated inductances, current and voltage sensing of motor terminal with estimated rotor angle for wide speed range. The rotor position is estimated rotor speed is determined by differentiation of the rotor position used only in the current model part of the flux observer for a low speed operation area. It does not requrie the knowledge of any montor paramenters, nor particular care for moter starting, In order to prove the suggested control algorithm, we have simulation and testing at actual experimental system. The developed sensorless control system is showing a good speed control response characterisitic result and high performance features in 20/1500 rpm with 1.0Kw RSM having 2.57 ratio of d/q reluctance.