• BMB Reports
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• v.29 no.5
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• pp.474-480
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• 1996

We present a study of evolutionary changes in expression of actin genes among closely related sea urchin species that exhibit different modes of early development. For this purpose, polyclonal antisera raised against peptides from the carboxyl terminus of the HeCyI cytoskeletal actin of Heliocidaris erythrogramma were used. H. erythrogramma is a direct developing sea urchin that proceeds from embryonic to adult stages without an intervening feeding larval stage. Expression patterns of the CyI actin isoform were compared with those of Heliocidaris tuberculata and to a related sea urchin Strongylocentrotus purpuratus, which both produce a feeding pluteus larval stage. The CyI actin of all three species is expressed in the same cell types. However, its expression patterns have been changed with reorganization of early cell lineage differentiation, which is apparent among the three species. Thus. evolutionary changes in CyI actin gene expression patterns are correlated with not only phylogenetic relationship, but developmental mode. The implication of this observation is that evolutionary changes in expression patterns of histospecific genes may underlie the emergence of novel developmental processes.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1455-1457
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• 2008

Cell adhesion is a coordinated process involving initial binding of integrin receptors to extracellular matrix (ECM), recruitment of adhesion proteins, and focal adhesion assembly. The formation of mechanically stable focal adhesion assembly of cells within surrounding ECM is a key parameter to direct numerous cellular functions including cell migration, differentiation, and apotosis. With current cell adhesion assays, it is difficult to understand contributions of each coordinated event on evolution of cell adhesion strengthening since cells spontaneously spread upon their adhesion to the substrate, thus remodeling their cytoskeletal structure. In this presentation, novel approaches for analysis of cell adhesion strengthening process based on the combination of mechanical device, micro-patterned substrates, and molecular biological techniques will be discussed.

• Proceedings of the KSME Conference
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• 2000.11a
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• pp.602-606
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• 2000

A general procedure for the design sensitivity analysis of structural dynamic problems has been presented in frame of the FRF-based substructuring formulation. In the procedure, the direct differentiation method is used for the sensitivity formula. For a system response function, the proposed method gives a parametric design sensitivity formula in terms of the partial derivatives of the connection element properties and the transfer matrix of the subsystems. The derived design sensitivity formula is applied to a numerical example. The comparison of sensitivities derived by the proposed method and the finite difference method shows that the proposed method is efficient and accurate.

• International Journal of Aeronautical and Space Sciences
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• v.2 no.1
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• pp.20-27
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• 2001

Aerodynamic sensitivity analysis is performed for the Navier-Stokes equations coupled with two-equation turbulence models using a discrete adjoint method and a direct differentiation method respectively. Like the mean flow equations, the turbulence model equations are also hand-differentiated to accurately calculate the sensitivity derivatives of flow quantities with respect to design variables in turbulent viscous flows. The sensitivity codes are then compared with the flow solver in terms of solution accuracy, computing time and computer memory requirements. The sensitivity derivatives obtained from the sensitivity codes with different turbulence models are compared with each other. The capability of the present sensitivity codes to treat complex geometry is successfully demonstrated by analyzing the flows over multi-element airfoils on Chimera overlaid grid systems.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.2
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• pp.209-217
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• 2003

In this paper, the dynamic system modeling and the state sensitivity analysis of the spin-coater system are proposed for the reduction of the vibration. In the respect of modeling, the spin-coater system is considered to be composed of servomotor, spindle, supporting base and so on. Each component of model is combined and derived to 3 dimensional equations. The combined model is verified by experimental values of actual system in the frequency domain. By direct differentiation of the constraint equations with respect to kinematic design variables, such as eccentricity of spindle, moment of inertia, rotational stiffness and damping of supported base, sensitivity equations are derived to the verified state equations. Sensitivity of design variables could be used for vibration reduction and natural frequency shift in the frequency domain. Finally, dominant design variables are selected from the sensitivity analysis.

• Computational Structural Engineering
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• v.7 no.3
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• pp.115-122
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• 1994

Design sensitivity analysis of the second order perturbed eigenproblems for random structural system is presented. Dynamic response of random system including uncertainties for the design variable is calculated with the first order and second order perturbation method to original governing equation. In optimal design methods, there is fundamental requirement for design gradients. A method for calculating the sensitivity coefficients is developed using the direct differentiation method for the governing equation and first order and second order perturbed equation.

• Interaction and multiscale mechanics
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• v.1 no.3
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• pp.339-355
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• 2008

A Galerkin meshfree method is presented for analyzing shear deformable cylindrical panels. Based upon the analogy between the cylindrical panel and the curved beam a pure bending mode for cylindrical panel is rationally constructed. The meshfree approximation employed herein is characterized by an enhanced moving least square or reproducing kernel basis function that can exactly represent the pure bending mode and thus meets the requirement of Kirchhoff mode reproducing condition. The variational form is discretized using the efficient stabilized conforming nodal integration with a smoothed nodal gradient based curvature. The resulting meshfree formulation satisfies the integration constraint for bending exactness. Moreover, it is shown here that the smoothed gradient preserves several desired properties which are valid for the standard gradient obtained by direct differentiation, such as partition of nullity and reproduction of a constant strain field. The efficacy of the proposed approach is demonstrated by two benchmark cylindrical panel examples.

• The Korean Journal of Zoology
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• v.35 no.1
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• pp.29-36
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• 1992

The present study describes the production of monoclonal antibodies against cultured chick myoblast to pursue critical proteins in muscle cell fusion. Among a panel of monoclonal antibodies, three, Mll-3H 13, Mll-3Hl8 and Mll-3H35 were inhibited movblast fusion. A single 101-kDa antigen reactive with monoclonal antibody Mll-3H35 was detected by radioimmu-noprecipitation or by immunoblotting. During the course of myogenesis, the level of the protein remarkably decreased as the cells there differentiated. These results suggest that the protein platys a direct role in the process of myoblast fusion mechanism.

• Molecules and Cells
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• v.41 no.8
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• pp.705-716
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• 2018

The endoplasmic reticulum (ER) is a critical organelle for protein synthesis, folding and modification, and lipid synthesis and calcium storage. Dysregulation of ER functions leads to the accumulation of misfolded- or unfolded-protein in the ER lumen, and this triggers the unfolded protein response (UPR), which restores ER homeostasis. The UPR is characterized by three distinct downstream signaling pathways that promote cell survival or apoptosis depending on the stressor, the intensity and duration of ER stress, and the cell type. Mammalian cells express the UPR transducers IRE1, PERK, and ATF6, which control transcriptional and translational responses to ER stress. Direct links between ER stress and immune responses are also evident, but the mechanisms by which UPR signaling cascades are coordinated with immunity remain unclear. This review discusses recent investigations of the roles of ER stress in immune responses that lead to differentiation, maturation, and cytokine expression in immune cells. Further understanding of how ER stress contributes to the pathogenesis of immune disorders will facilitate the development of novel therapies that target UPR pathways.

• Journal of Electrical Engineering and Technology
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• v.4 no.1
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• pp.49-54
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• 2009

One of the major challenges confronting a multiservice electric utility is the establishment of the right prices for its services. The key objectives of particular pricing schemes are reasonableness of company earnings, economic efficiency, the responsiveness of supply and of the allocation of sources to the desires of consumers, and maintenance of some degree of competition. This paper proposes a value-based pricing mechanism amenable to the current deregulation situation in electricity market allowing service differentiation. The proposed pricing mechanism can be implemented in a nodal auction model, and can also be applied to direct load control.