• Advances in Traditional Medicine
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• v.6 no.1
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• pp.34-38
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• 2006

The hot water extract of the seeds of Vernonia anthelmintica Kuntze (Compositae) in a dose of 10 ml/kg body weight of mice, showed significant analgesic activity on the hot plate analgesic method throughout the 4 h experimental period. The hole cross scores and the climbing out scores are of lower than the control animals. The hot water extract reduced the defecation in hole board study and significantly reduces the exploratory ambulation and head dipping behaviour. The seed also showed significant depressant activity on the exploratory ambulation of the open field scores. The drug decreased the spontaneous locomotion activity on brick-chip displacement method up to 45 min and also showed the ability to lessen the amphetamine induced hyperactivity up to 20 min. But it didn't show any effect on pentobarbital induced sleeping time test. The extract reduced gastrointestinal motility.

• Korean Journal of Veterinary Research
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• v.37 no.1
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• pp.203-212
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• 1997

Antibacterial efficacy and safety of copper sulfate pentahydrate (5% solution) was evaluated in laboratory and field studies for disinfection of some fish bacterial pathogens in cultured fish, flounder(Paralichthys olivaceus), mirror carp(Cyprinus carpio), gold fish(Carrassius auratus) and eel(Anguilla japonica). Dipping of fishes for acute toxicity was performed for a period of 48h, and the TLm value(median tolerance limit) were 4.0ppm in mirror carp and flounder, 4.4ppm in rainbow trout, and 10.0ppm in gold fish. After exposure below than TLm value for 1 month, no side effects and histopathological changes were observed. The test drug were shown high antibacterial activities against most bacterial pathogens by exposure in the laboratory and field trials. However, Aeromonas salmonicida showed a resistance to the dosage. We can prevent outbreaks of bacterial diseases of fish by the dosage of 1ppm for 6hr and the test drug is thought to be used effectively and widely as a disinfectant against most bacterial fish pathogens.

• Journal of fish pathology
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• v.28 no.1
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• pp.37-42
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• 2015

In the present study, we performed a dipping of olive flounder (average length and weight: $20{\pm}2.0cm$, $70{\pm}5.0g$) for a period of three hours a day, over two days, in a melted complex of oxytetracycline (OTC) and neomycin (N), by dissolving 25-10 ppm or 50-20 ppm in water. Subsequently, the remaining antibiotic density in muscle tissue collected from olive flounder was investigated, 1, 5, 14 and 40 days after discontinuation of the medication. 5 fish were used from each group. The standard graph drawn from the results of diluting two standard solutions of OTC and N based on various density levels, showed a relatively straight line with an $R^2$ of 0.9999 and 0.9952, respectively. The recovery rate of OTC was shown to be 90-93% and N, 88-95%. Upon measurement of the remaining antibiotic density in the test group that had been exposed to 25-10 ppm of the complex of OTC and N, $0.97{\pm}0.084{\mu}g/ml$ of OTC and $0.118{\pm}0.079{\mu}g/ml$ N were detected on 1 day of the test. No antibiotic density was detected after day 5 of the test. Regarding the test group that were exposed to 50-20 ppm of the complex of OTC and N, $1.324{\pm}0.062{\mu}g/ml$ of OTC and $0.788{\pm}0.05{\mu}g/ml$ N were detected on day 1 of the test, and no antibiotic density was detected after day 5 of the test.

• The Journal of Natural Sciences
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• v.6 no.1
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• pp.55-64
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• 1993

Experiments were conducted to study the effect of auxins and cultural factors on rooting characteristics in offshoots cutting of Aloe vera. The result obtained were as follows;1. Short offshoots(15-20cm) with rhizome had more roots than that of the removed rhizome, while long offshoots(25-30cm) without rhizome had more roots and heavier root dry weight than short offshoots with rhizome. The highest rooting ability occured when IBA treated 500mg per liter in short offshoots with rhizome, whereas long offshoots without rhizome showed higher rooting ability at the level of 1,000mg/l of IBA and 200mg/I of NAA2. Among three kinds of anxin treated, both IBA and NAA showed better rooting ability than ethychlozate. Root number, root length and root dry weight were higher at 10 min. dipping treatment of IBA 500mg/I for short off-shoots without rhizome and 10 min. dipping treatment of IBA 1,000mg/l for long offshoots with rhizome. Rooting ability of long offshoots was higher than that of short offshoots.3. In addition to IBA 1,000mg/l, 7-day dryness under shading condition increased root number and dry weight of root in offshoots without rhizome. Control treatment lengthened root by increasing drying period under the shading condition.4. The plain soil with mamure had not only higher root dofferentiation but higher root growth of offshoots cutting than the artificial soil.5. Rooting ability in offshoots cutting had a tendency increasing with decrease in shading percentage to natural. Black color poly ethylene film mulching had better rooting ablility than transparency poly ethlene film mulching in offshoots cutting.

• Korean Journal of Food Science and Technology
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• v.9 no.1
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• pp.13-18
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• 1977

In order to improve the storage ability of Spur Red Delicious apple, calcium ingredients were applied to the apple tree during the growing period, and then the effects of calcium on appearances and respiratory activity of harvest apples were investigated during the storage period. The results of this work were summarized as follows: 1. The freshness of apples treated by calcium compounds reached $73{\sim}78%$, but that of control group was 68% by March of next year. 2. The respiratory rate of the apples that had been applied the high concentration of ammonium sulfate in field during the growing period, was accelerated rather than control group, and that of calcium treatment groups was continuously inhibited during the storage period. 3. The direct foliar application of calcium compounds in the field was more effective than dipping apples in calcium chloride solution for better quality and improving storage ability after harvest. 4. The freshness and hardness relatively not much decreased in calcium treatment groups even after 5 month storage.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.427-431
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• 2013

Paprika fruits should be stored and distributed at above $7^{\circ}C$ to prevent chilling injury but the small amount of paprika that transports with other horticultural products in refrigerated container by ship usually stored less than $5^{\circ}C$ for other products. In this case, paprika fruits cannot help exposing chilling temperature, so that the paprika must be lost marketable value during a long period of transfer. This study was conducted to compare the alleviated effects of high $CO_2$ treatment (passive MAP), heat (hot water dipping), and UVc treatment on chilling injury of paprika fruits due to low temperature storage, and also to decide if these treatments can be used for transporting under $5^{\circ}C$. After each treatment the paprika were put in the low temperature storage ($4^{\circ}C$) for 20 days and afterwards change the in room temperature ($20^{\circ}C$) for 5 days. The fresh weight loss of all the treatments except the high $CO_2$ treatment showed around 7~12% after 25 days of storage and the ethylene concentration showed periodical increases and decreases as around 3 ${\mu}l/l$. The $CO_2$ concentration was rapidly increased 33% carbon dioxide in high $CO_2$ treatment during room temperature storage after cold storage for 20 days. The firmness which is key quality characteristics during storage and is decreasing caused by chilling injury was not significantly different among all treatments. However, the firmness of stored paprika was maintained highest in the treated with hot water dipping. Therefore, HWD and UVc treatment that showed 60% of electrolyte leakage in the $4^{\circ}C$ control (chilling injury control) and similar level with the $7^{\circ}C$ control (non-chilling injury control) would be effective to alleviate chilling injury in the stored paprika.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.1
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• pp.41-46
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• 1994

This study was undertaken to clarify the effect of killing methods on physical and rheological changes of plaice, Paralichthys olivaceus muscle at early period after death. Plaices killed by the four different methods(1. spiking at the brain instantly. 2. drowning in air. 3. dipping in 1,000ppm ethylaminobenzoate dissolved sea water as an anesthetic. 4. electrifying in sea water.) were stored at $5^{\circ}C$, and the rigor-index and breaking strength through storage were monitored. The longest onset time of rigor-mortis and full rigor was in the samples killed by dipping in sea water with dissolved anesthetic among all samples, where rigor-mortis began at 20hrs after killing and maximum tension was attained after 56hrs. However, in the cases of plaice electrified in sea water or drowned in air, the onset of rigor-mortis began just after killing and maximum tensions were attained after 9hrs and 13hrs, respectively. The level of breaking strength in the muscle of fish killed by spiking the brain instantly was $950.30{\pm}50.23g$, immediately after killing. The value and time reached around the maximum breaking strength for each of the samples were $1,230.60{\pm}30.32g$ and Ohr (immediately after killing) for samples killed by electrifying in sea water, $1,235.83{\pm}35.37g$ and 2.5hrs for drowning samples, $1,186.29{\pm}55.90g$ and 10hrs for spiking samples, and $1,189.67{\pm}50.32g$ and 15hrs for samples dipped in anesthetic, respectively. From the results above, it could be concluded that electrification in sea water is the most effective method in accelerating rigor-mortis and shortening times of reaching the maximum breaking strength of fresh plaice flesh of all the killing methods at early periods after death, whereas dipping in sea water treated with anesthetic was the most effective way in delaying those changes.

• Korean journal of applied entomology
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• v.16 no.1 s.30
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• pp.47-53
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• 1977

Bacterial diseases of soybean has been recognized as a limiting factor of soybean production in Korea as it was estimated to cause around 10 percent of yield losses annually. The purpose of the study is to obtain information on the diseases through proving the kinds of pathogens and epidemiology, The wire brush method and multineedle appeared to be the best way of inoculation under all circumstances. Wire brush method, especially, was effective in shortening the incubation period and manifesting the lesion development by introducing more inoculum per unit of area. In case of spray inoculation it was necessary to apply a small amount(1 : 1,000) of wetting agent, twin-20, otherwise it was unabled to produce the diseases under field conditions. Two kinds of bacterial diseases caused by Pseudomonas glycinea and Xanthomonas phaseoli var. sljense were found from surveyed areas in Kore. Wild fire disease on soybean caused by Pseudomonas tabaci had not detectable during the experiment although there were several reports on the disease from other countries. when the pathogens were introduced into sterile soil, bacterial leaf blight pathogen could exsisted until 30 days while bacterial pustule pathogen survived only 4 days under the natural conditions of later June. Both bacteria, however, could produce the disease after more than 10 months period of storage in refrigerator when they were exsisted in infected plant tissues. In warehouse, non-temperature controlled, the bacteria lose their infectability within 6 months period from October to April even though they exsisted in infected tissues. Surface infested seeds with the pathogens could not produced the diseases on seedling stages of soybean plants when the seeds were planted in sterile soil after inoculation by dipping the seeds into bacterial suspensions, although germination was depressed by the pathogens when the seeds were planted on agar media.

• Journal of Food Hygiene and Safety
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• v.28 no.3
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• pp.213-216
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• 2013

Antibiotic Detection Kit (Combination I), a lateral flow immunoassay (LFIA) developed for the detection of antibiotic residues in milk, was utilized for the analysis of antibiotic residues in the muscle tissue of olive flounder. After 60-min treatment by dipping in water dosed with ampicillin (200-g/ton water), the residue depletion of ampicillin was investigated in 25 cultured olive flounder (Paralichthys olivaceus). Muscles of fish were sampled on the 1st, 2nd, 3rd, 4th and 5th day after drug treatment. The concentration of ampicillin in the muscle was determined by LFIA. The absorbance ratio of the sample to the control blank (Bs/Bo) was employed as an index to determine the muscle residues in olive flounder. To investigate the recovery rate, standard solutions were added to muscle samples to give final concentrations in the muscle of 4 and 8 ng/ml. The recovery rates of all spiked samples were > 96% of the spiked value. Ampicillin was detected in the muscle of fish treated with the drug until the 2nd day of the withdrawal period. The present study showed that the LFIA can be easily adopted to predict ampicillin residues in tissue of farmed fishes.

• Korean journal of food and cookery science
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• v.21 no.5
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• pp.703-708
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• 2005

The purposes of this study were to establish washing conditions for vegetable salad to reduce the microbial hazard by using sodium hypochlorite solution and eventually to implement HACCP for salad processing. By using the salad production line of Shinkeum Co. located in Gwacheon, Gyunggi-do, salad samples were washed under several washing conditions (chlorine dip period, chlorine concentration, rinse time, etc.) to determine the most effective conditions. The original washing line consisted of 3 baths (100 ppm chlorine water dip, water rinse, and water rinse), each with a capacity of 100 L of tap water and 5 kg of salad. First, the salad samples were washed with 100 ppm of sodium hypochlorite solution for various dip times (3, 6, 9, 12 min); however, only a 1 log- or less-reduction in total microbial counts was achieved in all groups and the time of chlorine water dip was not a significant factor in reducing the microbial hazard. When another water bath was added before the chlorine water dip (4-bath washing), a 2 log-reduction in total microbial counts was achieved. This result suggested the importance of pre-dipping salad materials in water before chlorine treatment to reduce the organic load on the surface of the vegetables. Coliforms were not detected at all after washing. As the concentration of chlorine $(50{\sim}150\;ppm)$ and rinse time $(0.5{\sim}2\;min)$ increased, greater microbial reduction was achieved; however, physical damage of the salad was observed. Finally, the optimum washing conditions for salad were determined as 3 min-water dip, 3 min-chlorine (100 ppm) dip, 2 min-rinse, and 2 min-rinse.