Muscle distribution level after dipping administration of a combination of oxytetracycline and neomycin in olive flounder, Paralichthys olivaceus

In the present study, we performed a dipping of olive flounder (average length and weight: $20{\pm}2.0cm$, $70{\pm}5.0g$) for a period of three hours a day, over two days, in a melted complex of oxytetracycline (OTC) and neomycin (N), by dissolving 25-10 ppm or 50-20 ppm in water. Subsequently, the remaining antibiotic density in muscle tissue collected from olive flounder was investigated, 1, 5, 14 and 40 days after discontinuation of the medication. 5 fish were used from each group. The standard graph drawn from the results of diluting two standard solutions of OTC and N based on various density levels, showed a relatively straight line with an $R^2$ of 0.9999 and 0.9952, respectively. The recovery rate of OTC was shown to be 90-93% and N, 88-95%. Upon measurement of the remaining antibiotic density in the test group that had been exposed to 25-10 ppm of the complex of OTC and N, $0.97{\pm}0.084{\mu}g/ml$ of OTC and $0.118{\pm}0.079{\mu}g/ml$ N were detected on 1 day of the test. No antibiotic density was detected after day 5 of the test. Regarding the test group that were exposed to 50-20 ppm of the complex of OTC and N, $1.324{\pm}0.062{\mu}g/ml$ of OTC and $0.788{\pm}0.05{\mu}g/ml$ N were detected on day 1 of the test, and no antibiotic density was detected after day 5 of the test.

Oxytetracycline과 neomycin 복합제의 약욕에 따른 양식 넙치(Paralichthys olivaceus)의 근육내 잔류량 변화

본 연구에서는 넙치 (평균체장 및 무게: $20{\pm}2.0cm$, $70{\pm}5.0g$)에 옥시테트라사이클린(oxytetracycline, OTC)-네오마이신(neomycin, N) 복합제를 25-10 ppm 및 50-20 ppm으로 용해하여 2일 동안, 3시간씩 약욕을 실시하였고, 약물투여 중지 후 1일, 5일, 14일, 40일째에 시험군별로 각각 5마리씩 넙치의 근육을 채취하여 항생제 잔류농도를 조사하였다. OTC와 N 표준용액을 농도별로 희석하여 표준곡선을 작성한 결과 각각 $R^2=0.9999$, $R^2=0.9952$로 양호한 직선성을 나타내었고, OTC와 N 회수율은 각각 90~93%, 88~95%가 관찰되었다. 잔류농도에 대한 측정결과, OTC와 N 복합제를 25-10 ppm 투여한 시험군에서 1일째 OTC는 $0.97{\pm}0.084{\mu}g/ml$이 검출되었고, N은 $0.118{\pm}0.079{\mu}g/ml$이 검출되었으나 5일째 이후부터 검출이 되지 않았다. OTC와 N 복합제를 50-20 ppm 투여한 시험군에서 1일째 OTC는 $1.324{\pm}0.062{\mu}g/ml$이 검출되었고 N은 $0.788{\pm}0.05{\mu}g/ml$이 검출되었으나 5일째 이후부터 검출이 되지 않았다.