• Journal of Microbiology and Biotechnology
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• v.29 no.1
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• pp.55-58
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• 2019

Development of stable rCHO cell lines is still time consuming and labor intensive, although it is a critical step in the commercial development of recombinant antibodies. The current work demonstrates, for the first time, that electroporation of CHO cells with DMSO can enhance stable expression of recombinant antibodies in rCHO cells. Electroporation with DMSO resulted in an average 3.7-fold and 2.8-fold increases in expression levels of aflibercept and pembrolizumab, respectively, in pools of stable rCHO cells. It also resulted in an average of 2.2-fold and 2.6-fold increases in the expression of aflibercept and pembrolizumab, respectively, in single-cell derived rCHO clones. Simple batch cultures of rCHO cell clones with the highest expression produced 1.0 g/l for aflibercept and 1.4 g/l for pembrolizumab without a time-consuming gene amplification process. Electroporation with DMSO also shortened the development of rCHO cell lines to 2-3 months, allowing rapid establishment of stable rCHO cell lines with a desirable expression level antibodies.

• Textile Coloration and Finishing
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• v.15 no.2
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• pp.68-75
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• 2003

In this work, ultrafiltration(UF) membranes were prepared using polyethersulfone(PES). The polymer was dissolved in various solvent, such as N, N-dimethyl formamide(DMF), N,-dimethyl acetamide (DMAc), N,N-dimethyl sulfoxide(DMSO) and N-methyl-2- pynolidone(NMP). Each polymer solution was casted on the glass plate, and immersed into non-solvent bath. In this way finely porous UF membranes were prepared by phase inversion method. The cross sectional structure of PES membrane was asymmetric which was consist of sponge-like sublayer, finger-like toplayer, and active skin layer. From the solute rejection experiments, the molecular weight cut off of the prepared membrane in various solvent was evaluated 10,000 for DMF, 30,000 for DMAc, 50,000 for DMSO, and 10,000 for NMP respectively.

• Development and Reproduction
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• v.15 no.4
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• pp.281-289
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• 2011

The objective of this study was to investigate the effective cryoprotectants for the cryopreservation of porcine mesenechymal stem cells (pMSCs). In order to understand the effectiveness of various cryoprotectants on pMSCs, we studied the most commonly used cryoprotectants; dimethyl sulfoxide (DMSO), ethylene glycol (EG), DMSO and EG. pMSCs were isolated from bone marrow matrix of piglet (2 month) and characterized by alkaline phopshatase (AP) activity, colony forming, and differentiation to adipocyte. In slow cooling cryopreservation, the pMSCs were exposed to cell medium containing Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% DMSO, 1.5M EG and 5% DMSO/0.75M EG, respectively, and freezed to $-1^{\circ}C$/min from $25^{\circ}C$ up to $-80^{\circ}C$ in a cryo-container. The proportion of viable cells and the growing rates in fresh pMSCs were significantly (P<0.05) higher than those of other groups, but did not differ between the cryopreserved groups. The expression of Sox-2 and Nanog gene was increased by extending culture time in cryopreserved groups. The expression of Bax gene in cryopreserved groups was similar with fresh pMSCs. Moreover, the gene expression of adipocyte-specific marker as well as chondrogenic/osteogenic factors in cryopreserved groups was similarly to fresh pMSCs. Taken together, our results suggested that all these cryoprotectants of 10% DMSO, 1.5M EG and 5% DMSO/0.75M EG could be used for cryopreservation of the pMSCs.

• The Korean Journal of Malacology
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• v.23 no.1
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• pp.51-57
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• 2007

This study examined the possibility on the dietary value and cryopreservation of the marine microalga, Isochrysis galbana. Four cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol (Gly) and 1.2-propanedial (PD) were tested at the concentrations of 1.0, 1.5, 2.0, 2.5 and 3.0 M respectively. The highest survival rates were obtained with 1.5 and 2.0 M of four cryoprotectants yielding a survival rate of 80%. Cell concentration of $30\;{\times}\;10^4\;cell/ml$ at the initial point of the experiment was increased to $365\;{\times}\;10^4\;cell/ml$ in 1.5 M of dimethyl sulfoxide, $298\;{\times}\;10^4\;cell/ml$ in 1.5 M of ethylene glycol, $512\;{\times}\;10^4\;cell/ml$ in 1.5 M of glycerol, and $385\;{\times}\;10^4\;cell/ml$ in 1.5 M of 1.2-propanedial after five days, respectively. In dietary value experiment, survival rate and growth were not significantly different.

• Journal of Life Science
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• v.27 no.12
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• pp.1462-1469
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• 2017

The purpose of this study was to investigate the effects of resveratrol supplementation on inflammasome, inflammation, and macrophage markers in subcutaneous adipose tissue of high-fat-diet-induced obese mice. C57BL/6 mice were randomly assigned to three groups: normal diet control (NC; n=10), high-fat diet control (HC; n=10), or high fat with resveratrol (HRE; n=10) group. The mice were fed a high-fat diet (60% of calories from fat) or normal diet (18% of calories from fat). Resveratrol dissolved in a 0.1ml solution of dimethyl sulfoxide was supplemented orally at 25 mg/kg body weight. After 15 weeks, the body weight was significantly higher in the high-fat diet group than in the normal diet group. The inflammasome markers NLRP3, ASC, and caspase1 were significantly lower in the HRE group than in the HC group. The levels of an inflammation marker, IL-18, were also significantly lower in the HRE group than in the NC and HC groups. The levels of macrophage markers F480 and CD86 were significantly lower in the HRE group than in the HC group. The levels of the M2 macrophage marker CD206 were significantly decreased in the HC and HRE groups. Resveratrol had a positive effect on ameliorating the complications of high fat diet-induced obesity by reducing inflammasome and M1 macrophage gene expressions. However, resveratrol supplementation did not reduce inflammation gene expression.

• Journal of the Korean Graphic Arts Communication Society
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• v.16 no.2
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• pp.61-74
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• 1998

Some photopolymer, poly(vinyl cinnamoyl acetate)(PVCiA) was synthesized by esterification of polymer(vinyl alcohol)(PVA) with monochloroacetic acid, followed by reaction poly(vinyl monochloroacetate)(PVAhA) and potassium cinnamate. When esterification of PVA with monochloroacetic acid was reacted in the dimethyl sulfoxide(DMSO), in the synthesis of PVChA, it is very good yield and the successive cinnamoyl acetoxyl esterification of PVCiA can be successfully synthesized. But PVCiA is low photosensitive polymer if net added photosensitizing dyes. Here, we synthesized photosensitizing dyes.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2008.04a
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• pp.95-97
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• 2008

In this study eco-friendly wax remover for printing table of flat screen was investigated. Seven organic solvents, diethylene glycol monomethyl ester, triethylene glycol monomethyl ester, triethylene glycol monobutyl ester, tripropylene glycol monomethyl ester, dimethyl sulfoxide(DMSO), trichloro ethylene(TCE), tolune, were used as resin wax remover. The resin wax solubility with solvents was investigated. DMSO was found to be the most suitable eco-friendly resin wax remover.

• Proceedings of the Korean Society of Rheology Conference
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• 2003.05a
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• pp.65-68
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• 2003

No Abstract, See Full Text

• Proceedings of the Korean Society of Rheology Conference
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• 2003.05a
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• pp.123-126
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• 2003

No Abstract, See Full Text

• Proceedings of the Korean Society of Rheology Conference
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• 2003.05a
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• pp.143-146
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• 2003

No Abstract, See Full Text