Efficient Development of Stable Recombinant Chinese Hamster Ovary (rCHO) Cell Lines to Produce Antibodies by Using Dimethyl Sulfoxide (DMSO) in Electroporation |
Byun, Juyoung
(PanGen Biotech Inc.)
Yoon, Sena (PanGen Biotech Inc.) Jeong, Yunji (Graduate School of Biotechnology, Kyung Hee University) Oh, Uitaek (Graduate School of Biotechnology, Kyung Hee University) Cho, Sujin (Graduate School of Biotechnology, Kyung Hee University) Park, Jeongsoo (PanGen Biotech Inc.) Jeong, Yongsu (Graduate School of Biotechnology, Kyung Hee University) Baek, Kwanghee (Graduate School of Biotechnology, Kyung Hee University) Yoon, Jaeseung (Graduate School of Biotechnology, Kyung Hee University) |
1 | Baldi L, Hacker DL, Adam M, Wurm FM. 2007. Recombinant protein production by large-scale transient gene expression in mammalian cells: state of art and future perspectives. Biotechnol. Lett. 29: 677-684. DOI |
2 | Bertschinger M, Schertenleib A, Cavey J, Hacker DL, Wurm FM. 2008. The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells. Mol Biotechnol. 40: 136-143. DOI |
3 | Steger K, Brady J, Wang W, Duskin M, Donato K, Peshwa M. 2015. CHO-S antibody titers >1 gram/liter using flow electroporation-mediated gene expression followed by rapid migration to high-yield stable cell lines. J. Biomol. Screen. 20: 545-551. DOI |
4 | Cervera L, Fuenmayer J, Gonzalez-Dominguez I, Gutierrez-Granados S, Segura MM, Godia F. 2015. Selection and optimization enhancer additives for increased virus-like particle production in HEK293 suspension cell cultures. Appl. Microbiol. Biotechnol. 99: 9935-9949. DOI |
5 | Melkonyan H, Sorg C, Klempt M. 1996. Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO). Nucleic. Acids Res. 24: 4356-4357. DOI |
6 | Ye J, Kober V, Tellers M, Naji Z, Salmon P, Markusen JF. 2009. High-level protein expression in scalable CHO transient transfection. Biotechnol. Bioeng. 103: 542-551. DOI |
7 | Daly TJ, Fandl JP, Papadopoulos NJ. 2006. Fusion protein capable of binding VEGF. US Patent publication No 7087411B2. |
8 | Yoon J, Baek K, Byun T, Park J. 2014. Expression vector animal cells. US Patent Publication No 20140038233. |
9 | Ciudad CJ, Urlaub G, Chasin LA. 1988. Deletion analysis of the Chinese hamster dihydrofolate reductase gene promoter. J. Biol. Chem. 263: 16274-16282. DOI |
10 | Paredes V, Park JS, Jeong Y, Yoon J, Baek K. 2013. Unstable expression of recombinant antibody during long-term culture of CHO cells is accompanied by histone H3 hypoacetylation. Biotechnol. Lett. 35: 987-993. DOI |
11 | Noh SM, Sathyamurthy M, Lee GM. 2013. Development of recombinant Chinese hamster ovary cell lines for therapeutic protein production. Curr Opin. Chem. Eng. 2: 391-397. DOI |
12 | Jesus MD, Wurm FM. 2011. Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors. Eur. J. Pharm. Biopharm. 78: 184-188. DOI |
13 | Durocher Y, Butler, M. 2009. Expression systems for therapeutic glycoprotein production. Curr. Opin. Biotechnol. 20: 700-707. DOI |
14 | Dietmair S, Nielsen LK, Timmins NE. 2010. Engineering a mammalian super producer. J. Chem. Technol. Biotechnol. 86: 905-914. DOI |