• Title/Summary/Keyword: dimers

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Occurrence of ELISA Inhibitors, and Inhibitor-free Determination of Cyclobutane Pyrimidine Dimers in Japanese Cypress Leaves

  • Hashimoto, Tohru;Nakagawa, Noriko;Okano, Michiaki;Nikaido, Osamu
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.448-450
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    • 2002
  • DNA samples extracted from Japanese cypress leaf tissues contain isopropyl alcohol-precipitable, high molecular weight compounds, which interfere ELISA for cyclobutane pyrimidine dimers (CPD). Removal of the compounds is achieved by DEAE ion-exchange column chromatography and improves the ELISA responses of the DNA. When extracting DNA repeatedly from the same leaf tissues, the DNA samples show CPD responses which increase with the order in sequential extraction, and hence for a reliable detennination of DNA lesion a thorough extraction of DNA is required. Clearing these two problems it was demonstrated that CPD level was slightly higher in the leaves of trees growing under full sunlight than in those growing under UBV -cut sunlight.

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Helical Compounds Forming Gas-Phase Dimers: A Dispersion-corrected Density Functional Investigation

  • Tongying, Pornthip;Sooksimuang, Thanasat;Tantirungrotechai, Yuthana
    • Bulletin of the Korean Chemical Society
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    • v.32 no.4
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    • pp.1231-1236
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    • 2011
  • Chiral discrimination is the ability to distinguish one enantiomeric form over another. The differential binding interaction between two molecules with the same helicity and those with the opposite helicity was investigated by using dispersion-corrected density functional theory. [5]helicene, tetrahydro[5]helicene and the polar D-${\pi}$-A compounds, 3,12-dimethoxy-7,8-dicyano-[5]helicene and 3,12-dimethoxy-7,8-dicyano-tetrahydro[5]helicene were the monomers considered in this study. In gas phase, the dimeric interaction from two helical molecules with the opposite handedness is greater than from those with the same handedness. The stable configurations of such dimers were identified. The most stable configuration tends to be the one with maximum contact between monomers.

SPECTROSCOPY OF PLANETS AND COMETS (거대 행성들과 혜성들의 분광학)

  • KIM SANG JOON;LEE YOUNG SIEK;YI YU;KIM YOUNG HA
    • Publications of The Korean Astronomical Society
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    • v.15 no.spc1
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    • pp.31-38
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    • 2000
  • We have presented detected molecules, atoms, radicals, ions, and dimers in the atmospheres of planets and comets from Earth-based and spaceborne observatories during the last 3 decades. We have reviewed spectroscopic studies on the auroral emissions and air glows of the giant planets, and briefly summarized spectroscopic observations of dimers in the atmospheres of the giant planets and Titan. In particular, we highlighted the recent detections of new molecular emissions and absorptions in the spectra of the giant planets, Titan, and recent bright comets from spaceborne or ground-based observatories. We also reviewed current models and theories of the origin and evolution of the solar system, and implications of isotopic ratios in these atmospheres.

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Thermal Dissociation and Conformational Lock of Superoxide Dismutase

  • Hong, J.;Moosavi-Movahedi, A.A.;Ghourchian, H.;Amani, M.;Amanlou, M.;Chilaka, F.C.
    • BMB Reports
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    • v.38 no.5
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    • pp.533-538
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    • 2005
  • The kinetics of thermal dissociation of superoxide dismutase (SOD) was studied in 0.05 M Tris-HCl buffer at pH 7.4 containing $10^{-4}\;M$ EDTA. The number of conformational locks and contact areas and amino acid residues of dimers of SOD were obtained by kinetic analysis and biochemical calculation. The cleavage bonds between dimers of SOD during thermal dissociation and type of interactions between specific amino acid residues were also simulated. Two identical contact areas between two subunits were identified. Cleavage of these contact areas resulted in dissociation of the subunits, with destruction of the active centers, and thus, lost of activity. It is suggested that the contact areas interact with active centers by conformational changes involving secondary structural elements.

Comparative studies of density functionals in modelling hydrogen bonding energetics of acrylamide dimers

  • Lin, Yi-De;Wang, Yi-Siang;Chao, Sheng D.
    • Coupled systems mechanics
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    • v.6 no.3
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    • pp.369-376
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    • 2017
  • Intermolecular interaction energies and conformer geometries of the hydrogen bonded acrylamide dimers have been studied by using the second-order Møller-Plesset (MP2) perturbation theory and the density functional theory (DFT) with 17 density functionals. Dunning's correlation consistent basis sets (up to aug-cc-pVTZ) have been used to study the basis set effects. The DFT calculated interaction energies are compared to the reference energy data calculated by the MP2 method and the coupled cluster method at the complete basis set (CCSD(T)/CBS) limit in order to determine the relative performance of the studied density functionals. Overall, dispersion-energy-corrected density functionals outperform uncorrected ones. The ${\omega}B97XD$ density functional is particularly effective in terms of both accuracy and computational cost in estimating the reference energy values using small basis sets and is highly recommended for similar calculations for larger systems.

Quantification for the Distribution of Hydrogen Bonding Species in Phenolic Model Compounds and Polybenzoxazines (페놀계 모델 화합물 및 폴리벤조옥사진 수지에 대한 수소결합분포의 정량화)

  • Kim, Ho-Dong;Moon, Hwa-Yeon
    • Textile Coloration and Finishing
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    • v.20 no.4
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    • pp.21-30
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    • 2008
  • To understand the complex hydrogen bonding structure, several phenolic derivatives and benzoxazine model compounds are synthesized and characterized by Fourier transform infrared spectroscopy (FT-IR). The estimation of molar extinction coefficients for various types of hydrogen bonding species is systematically carried out by the curve-resolving of FT-IR spectra. The distribution of hydrogen bonding species in benzoxazine model dimers is quantitatively analyzed. It is revealed that benzoxazine dimers and BA-a polybenzoxazine are mainly composed of intramolecular interaction rather than intermolecular interaction.

Isolation and Structure Elucidation of Proanthocyanidin in Bark of Pinus densiflora (소나무수피 프로안토시아니딘(Proanthocyanidin)의 분리 및 구조분석)

  • Song, Hong-Keun;Oh, Sung-Jin
    • Journal of the Korean Wood Science and Technology
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    • v.24 no.2
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    • pp.81-93
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    • 1996
  • To elucidate the structure of procyanidin in Korean red pine (Pinus densiflora S. et Z.), bark, the extractives were extracted with acetone-water mixture(7:3, v/v) from inner bark of Korean red pine. The extracts separated three fractions which were extracted by liquid-liquid extraction. The extracting solvents were chloroform and ethyl acetate and water. The part of ethylacetate soluble was chromatographed by liquid chromatography. The ethylacetate soluble portion yielded four natural procyanidin dimers, two known epicatechin-($4{\beta}{\rightarrow}6$)-catechin, catechin-($4{\alpha}{\rightarrow}8$)-catechin and two unknown catechin-($4{\beta}{\rightarrow}6$)-catechin and conformational isomer of epicatechin-($4{\alpha}{\rightarrow}6$)-catechin. The additional catechins was also isolated. The structures of these procyanidins were elucidated by their $^{13}C$-NMR spectra.

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Binding Symmetry of External Divalent Cations to Cyclic Nucleotide-gated IonChannel Reveled by Channel Tandem Dimers

  • Kwon, Ryuk-Jun;Park, Chul-Seung
    • Proceedings of the Korean Biophysical Society Conference
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    • 2001.06a
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    • pp.37-37
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    • 2001
  • Cyclic nucleotide-gated (CNG) channels are composed of homo or hetero tetramer of ${\alpha}$ and ${\beta}$ subunits. The a subunits of these channels have a conserved glutamate residue within the pore-forming region. This residue determines the selectivity as well as the affinity for the extracellular divalent cations. Using the high affinity mutant (E363D) of bovine retinal CNG channel in which the Glu was replaced to Asp at position 363, we constructed tandem dimers and investigated the binding symmetry of divalent cation to the site.(omitted)

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Oligomer Complexes of the (VQIVYK + NNQQNY) and (VQIVYK + LYQLEN) Mixing Solutions

  • Jung, Yeon-Ji;Shin, Min-Ji;Kim, Ho-Tae
    • Mass Spectrometry Letters
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    • v.10 no.1
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    • pp.32-37
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    • 2019
  • The ${\pi}-{\pi}$ interactions of the peptide-dimer and peptide-trimer complexes were investigated in the (VQIVYK + LYQLEN) and (VQIVYK + NNQQNY) mixing solutions. The results showed that tyrosine (Y) residues were critical in the formation of hetero peptide-dimers and -trimers during the early oligomerization process. We used collision-induced dissociation (CID) along with electrospray ionization mass spectroscopy (ESI-MS) to obtain the structural information of the hetero-dimers and -trimers. We chose three amyloidogenic peptides-VQIVYK, NNQQNY, and LYQLEN-from tau protein, yeast prion-like protein Sup35, and insulin chain A, respectively. Hetero-dimer, -trimer, -tetramer, and -pentamer complexes were observed in the mass spectra. The tandem mass spectrum of the hetero-dimer and hetero-trimer showed two different fragmentation patterns (covalent and non-covalent bond dissociation). Y-Y interaction structures were also proposed for the hetero-dimer and -trimer complexes.