• 대한전자공학회논문지
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• 제23권2호
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• pp.278-284
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• 1986

There are considerabel interests in the use of acoustical lengths for characterization of scattered data required in diffraction tomography. In this paper, we present two new methods, calculation of acoustical lengths by Hibert transform and estimate on of integrated values on the scan lines from calcualted vales. These techniques offer insight into the acquisition of projection data in diffraction tomography. The validity of the proposed methods has bee confirmed by computer simulation.

• 대한전자공학회논문지
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• 제24권1호
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• pp.167-172
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• 1987

In this paper, the interpolation schemes for Direct Fourier Reconstruction in Diffraction Tomography are discussed. The interpolator using circular sampling theorem is modified so that the reconstructed image may be closer to original object than those produced with other interpolators. Reconstructed images obtained by computer simulations with several interpolators including that derived in this paper are compared to original object: two concentric cylinders.

• Applied Microscopy
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• 제47권4호
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• pp.223-225
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• 2017

Cryo-electron microscopy (cryo-EM) is arguably the most powerful tool used in structural biology. It is an important analytical technique that is used for gaining insight into the functional and molecular mechanisms of biomolecules involved in several physiological processes. Cryo-EM can be separated into the following three groups according to the analytical purposes and the features of the biological samples: cryo-electron tomography (cryo-ET), cryo-single-particle reconstruction, and cryo-electron crystallography. Cryo-tomography is a unique EM technique that is used to study intact biomolecular complexes within their original environments; it can provide mechanistic insights that are challenging for other EM-methods. However, the resolution of reconstructed three-dimensional (3D) models generated by cryo-ET is relatively low, while single-particle reconstruction can reproduce biomolecular structures having near-atomic resolution without the need for crystallization unless the samples are large (>200 kDa) and highly symmetrical. Cryo-electron crystallography is subdivided into the following two categories according to the types of samples: one category that deals with two-dimensional (2D) crystalline arrays and the other category that uses 3D crystals. These two categories of electron-crystallographic techniques use different diffraction data obtained from still diffraction and continuous-rotation diffraction. In this paper, we review crystal-based cryo-EM techniques and focus on the recently developed 3D electron-crystallographic technique called microcrystal-electron diffraction.

• 한국음향학회지
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• 제18권5호
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• pp.83-90
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• 1999

본 논문에서는 초음파 회절 토모그라피를 위한 FBP와 BFP 영상복원 알고리즘에 관한 분해능을 연구하였다. 고정좌표계를 사용한 수정된 FBP 영상복원 알고리즘과 평면구조물에 적합한 BFP 영상복원 알고리즘을 이용하여 복원할 수 있는 토모그라픽 영상에 대한 분해능을 분석할 수 있는 모호함수를 유도하고 모의실험을 통하여 얻은 측방향 및 축방향 분해능을 분석하였다. 분석결과, FBP 영상복원 알고리즘에 대한 측방향 및 축방향의 3dB분해능은 각각 0.27파장, 0.70파장을 얻었으며, 또한 BFP 영상복원 알고리즘에 대한 측방향 및 축방향 분해능도 각각 0.39파장과 0.98파장으로 정량적으로 결정하였다. 따라서 본 연구를 통하여 수정된 FBP 영상복원 알고리즘과 BFP 영상복원 알고리즘은 회절 토모그라피를 위한 영상복원에 유용하게 이용할 수 있음을 확인하였다.

• Molecules and Cells
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• 제44권11호
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• pp.851-860
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• 2021

Label-free optical diffraction tomography (ODT), an imaging technology that does not require fluorescent labeling or other pre-processing, can overcome the limitations of conventional cell imaging technologies, such as fluorescence and electron microscopy. In this study, we used ODT to characterize the cellular organelles of three different stem cells-namely, human liver derived stem cell, human umbilical cord matrix derived mesenchymal stem cell, and human induced pluripotent stem cell-based on their refractive index and volume of organelles. The physical property of each stem cell was compared with that of fibroblast. Based on our findings, the characteristic physical properties of specific stem cells can be quantitatively distinguished based on their refractive index and volume of cellular organelles. Altogether, the method employed herein could aid in the distinction of living stem cells from normal cells without the use of fluorescence or specific biomarkers.

• 한국초전도학회:학술대회논문집
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• 한국초전도학회 2008년도 High Temperature Superconductivity Vol.XVIII
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• pp.20-20
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• 2008

• 전자공학회논문지A
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• 제29A권2호
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• pp.1-7
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• 1992

A consideration for image improvement under the Born approximation in the microwave diffraction tomography is suggested by using a projection function. The limiting factors in the degrading reconstructed image due to Born approximation are identified in terms of projection function and its modification is suggested to improve the degraded image based upon the Born approximation. In order to verify the proposed method, the reconstructed images are shown by computer simulation from the back-scattered data of angular and frequency diversity for squared dielectric cylinder with a various relative dielectric constant. From simulation results, it was shown that the proposed method can lead to a fairly good improved image for a severe degraded one irrespective of homogeneous and inhomogeneous dielectric object. In the future, the analysis on the limitation of this method should be considered and performed by means of more quantitative method.

• Current Optics and Photonics
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• 제2권5호
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• pp.460-467
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• 2018

Three-dimensional (3D) refractive-index (RI) imaging and quantitative analyses of angiosperm pollen grains are presented. Using optical diffraction tomography, the 3D RI structures of individual angiosperm pollen grains were measured without using labeling or other preparation techniques. Various physical quantities including volume, surface area, exine volume, and sphericity were determined from the measured RI tomograms of pollen grains. Exine skeletons, the distinct internal structures of angiosperm pollen grains, were identified and systematically analyzed.

• Current Optics and Photonics
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• 제4권4호
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• pp.330-335
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• 2020

We have investigated optimization of the working distance (WD) for a highly miniaturized imaging probe for endoscopic optical coherence tomography (OCT). The WD is the axial distance from the distal end of the imaging probe to its beam focus, which is demanded for dimensional margins of protective structures, operational safety, or full utilization of the axial imaging range of OCT. With an objective lens smaller than a few hundred micrometers in diameter, a micro-optic imaging probe naturally exhibits a very short WD due to the down-scaled optical structure. For a maximized WD careful design is required with the optical aperture of the objective lens optimally filled by the incident beam. The diffraction-involved effect was taken into account in our analysis of the apertured beam. In this study, we developed a simple design formula on the maximum achievable WD based on our diffraction simulation. It was found that the maximum WD is proportional to the aperture size squared. In experiment, we designed and fabricated very compact OCT probes with long WDs. Our 165-㎛-thick fiber-optic probes provided WDs of 3 mm or longer w ith reasonable OCT imaging performance.

• ETRI Journal
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• 제41권1호
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• pp.61-72
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• 2019

In this paper, we demonstrate the current concepts in holographic tomography (HT) realized within limited angular range with illumination scanning. The presented solutions are based on the work performed at Warsaw University of Technology in Poland and put in context with the state of the art in HT. Along with the theoretical framework for HT, the optimum reconstruction process and data visualization are described in detail. The paper is concluded with the description of hardware configuration and the visualization of tomographic reconstruction, which is calculated using a provided processing path.