• YAKHAK HOEJI
• /
• v.40 no.6
• /
• pp.690-696
• /
• 1996

It has been known that many kinds of cancer are caused by continued proliferation or abnormal differentiation. Thus, recent approaches to anticancer therapy have been focused on developing drugs that induce differentiation of cancer cells to normal cells. A typical differentiation agent, all trans-retinoic acid, is unsuitable for anticancer drug because all trans-retinoic acid produces unfavorable side effects and cytotoxicity in normal cells. Therefore, we have screened some new differentiation-inducing compounds obtained from marine organisms using F9 teratocarcinoma stem cells as a model system. We observed that fatty acid. glycolipid, saponin, sphingosine and sterol compounds of marine organisms had differentiation-inducing activity in F9 cells, were determined by morphological changes and Northern blot analysis. The expression of differentiation marker genes, such as laminin B1, type IV collagen and retinoic acid receptor beta were induced by treatment with those compounds.

• Communications of Mathematical Education
• /
• v.32 no.2
• /
• pp.131-146
• /
• 2018

Differentiation with integration is an important subject which is widely applied in mathematics, natural science, and engineering. Derivative is an important concept of differentiation. But students don't understand its concept well and concentrate on acquiring only the skill to solve the standardized calculus problem. So they are poor at understanding of the concept of differentiation. In this study, after making a survey of differentiation on college students, we try to analyze errors which appeared in solving differentiation problem and investigate mathematics process of limiting process inherent in the derivative and historical development about derivative. Thus, we try to analyze the understanding of differentiation and present the results about this.

• Development and Reproduction
• /
• v.13 no.3
• /
• pp.155-161
• /
• 2009

It has been reported that ganglioside GT1b is expressed during neuronal cell differentiation from undifferentiated mouse embryonic stem cells (mESCs), which suggests that ganglioside GT1b has a direct effect on neuronal cell differentiation. Therefore, this study was conducted to evaluate the effect of exogenous addition of ganglioside GT1b to an in vitro model of neuronal cell differentiation from undifferentiated mESCs. The results revealed that a significant increase in the expression of ganglioside GT1b occurred during neuronal differentiation of undifferentiated mESCs. Next, we evaluated the effect of retinoic acid (RA) on GT1b-treated undifferentiated mESCs, which was found to lead to increased neuronal differentiation. Taken together, the results of this study suggest that ganglioside GT1b plays a crucial role in neuronal differentiation of mESCs.

• Journal of Plant Biology
• /
• v.14 no.2
• /
• pp.15-21
• /
• 1971

The present paper was designed to investigate the effect of nitrogen, phosphorus and potassium on the histological differentiation of the young leaves of soybean (Glycinemax, M.). Observations were made on the numbers of lamina cells and lateral veins, width and thickeness of the lamina and vascularization of the midrib in the 5th leaf, and the differentiation of leaves at 42$\mu$ from the apical tips of the shoot apecis. Samples were taken at the time when the 2nd leaf was completed. The experimental plots were divided into twelve parts. And the results obtained are as follows. 1) Nitrogen stimulated the differentiation of the leaf, the vascuralization of the midrib and increased the numbers of lamina cells and lateral veins. 2) Phosphorus promoted the differentiation of lamina at the first stage of soybean growth. It was more effective in the plots of excessive application than otherwise. It had a small effect on the differentiation of lateral veins. 3) Among the elements, a deficiency of postassium resulted in a reduced differentiation of the lamina potassium had no effect on the thickening growth of the lamina and the differentiation of the midrib. 4) It appeared that phosphorus might compensate for the negative effect of potassium in the potassium and phosphorus plots.

• Journal of Families and Better Life
• /
• v.20 no.1
• /
• pp.1-16
• /
• 2002

The purpose of this study was to investigate the relationships of self-differentiation with conflict tactics behavior and marital adjustment among married men and women. Specifically, this study tried to examine the differences in the levels of conflict tactics behavior and of marital adjustment according to the levels of self-differentiation. The participants of the present study were 332 married men and 355 women who lived in Chonlabuk-Do province. The major results of this research were as follows. First, there was no significant difference in the level of total self-differentiation between men and women. There were significant differences in the degrees of sexually coercive conflict tactics behavior and marital adjustment between sexes. Second, results of difference analyses showed that there were significant differences in the levels of conflict tactics behaviors and of marital adjustment according to levels of self-differentiation. Finally, in the case of married men and women, the variable significantly affecting the conflict tactics behavior and the marital adjustment was the levels of self-differentiation. Based on the results as above, this research discussed the issue of the concept of self differentiation applied to Korean situation, and suggested some implications for family life education for decreasing conflict between spouses and for enhancing marital relationship.

• Research in Community and Public Health Nursing
• /
• v.18 no.4
• /
• pp.673-680
• /
• 2007

Purpose: The purpose of this study was to investigate the relation between self differentiation and school adjustment among adolescents. Methods: The subjects were 356 adolescents. The following research questions were established. The adolescents' self differentiation level was measured based on Bowen's family system theory modified for Korean adolescents(Je, 1989) and their school adjustment level was measured with developed for Korean adolescents(Park, 1998). Collected data were analyzed by Mean${\pm}$SD, t-test, ANOVA and Schefff and Pearson correlation coefficient (SPSS 12.0). Results: 1. The mean score for the level of self differentiation was $99.99{\pm}11.58$ and school adjustment was $63.52{\pm}8.80$. 2. The self differentiation level was different according to father's education and monthly income. The school adjustment level was different according to glade, sex father's education and father's occupation. 3. Self differentiation had a significant positive correlation with school adjustment. Conclusion: The self differentiation level was confirmed to have an influence on school adjustment. We need to develop a program that can enhance adolescents' self differentiation.

• BMB Reports
• /
• v.54 no.7
• /
• pp.374-379
• /
• 2021

Tetranectin (TN), an adipogenic serum protein, enhances adipocyte differentiation, however, its functional mechanism has yet to be elucidated. In the present study, we investigated the adipogenic function of TN by using medium containing TN-depleted fetal bovine serum (TN-del-FBS) and recombinant mouse TN (mTN). The adipocyte differentiation of 3T3-L1 cells was significantly enhanced by mTN supplementation essentially at differentiation induction, which indicated a potential role of the protein in the early differentiation phase. The adipogenic effect of mTN was more significant with insulin in the differentiation induction cocktail, implicating their close functional relationship. mTN enhanced not only the proliferation of growing cells, but also mitotic clonal expansion (MCE) that is a prerequisite for adipocyte differentiation in the early phase. Consistently, mTN increased the phosphorylation of ERK in the early phase of adipocyte differentiation. Results of this study demonstrate that the adipogenic function of mTN is mediated by enhancing MCE via ERK signaling.

• International Journal of Stem Cells
• /
• v.16 no.4
• /
• pp.406-414
• /
• 2023

Dedifferentiated fat cells (DFATs) isolated from mature adipocytes have a multilineage differentiation capacity similar to mesenchymal stem cells and are considered as promising source of cells for tissue engineering. Bone morphogenetic protein 9 (BMP9) and low-intensity pulsed ultrasound (LIPUS) have been reported to stimulate bone formation both in vitro and in vivo. However, the combined effect of BMP9 and LIPUS on osteoblastic differentiation of DFATs has not been studied. After preparing DFATs from mature adipose tissue from rats, DFATs were treated with different doses of BMP9 and/or LIPUS. The effects on osteoblastic differentiation were assessed by changes in alkaline phosphatase (ALP) activity, mineralization/calcium deposition, and expression of bone related genes; Runx2, osterix, osteopontin. No significant differences for ALP activity, mineralization deposition, as well as expression for bone related genes were observed by LIPUS treatment alone while treatment with BMP9 induced osteoblastic differentiation of DFATs in a dose dependent manner. Further, co-treatment with BMP9 and LIPUS significantly increased osteoblastic differentiation of DFATs compared to those treated with BMP9 alone. In addition, upregulation for BMP9-receptor genes was observed by LIPUS treatment. Indomethacin, an inhibitor of prostaglandin synthesis, significantly inhibited the synergistic effect of BMP9 and LIPUS co-stimulation on osteoblastic differentiation of DFATs. LIPUS promotes BMP9 induced osteoblastic differentiation of DFATs in vitro and prostaglandins may be involved in this mechanism.

• Animal cells and systems
• /
• v.3 no.2
• /
• pp.207-213
• /
• 1999

During the differentiation of Naegleria gruberi amoebas into flagellates, the amoebas undergo sequential changes in cell shape and form new cellular organelles. To understand the nature of the signal which initiates this differentiation and the signal transduction pathway, we treated cells with four agents, PMA, retinoic acid (RA), okadaic acid, and cAMP. Retinoic acid and cAMP had specific effects on the differentiation of N. gruberi depending on the time of the drug treatment. Addition of (100$\mu$M) retinoic acid at the initiation of differentiation inhibited differentiation by blockinq the transcription of differentiation specific genes (e.g., $\beta$-tubulin). This inhibition of differentiation by retinoic acid was overcome by co-treatment with cAMP (or dbcAMP, 20 $\mu$M). Addition of retinoic acid at later stages (30 and 70 min) had no effect on the transcriptional regulation of the $\beta$-tubulin gene, however the differentiation was inhibited by different degrees. Co-treatment of cAMP at these stages did not overcome the inhibitory effect of retinoic acid. These results suggest that the role of retinoic acid as a transcriptional regulator might be conserved throughout the evolution of eukaryotes.

• International Journal of Oral Biology
• /
• v.37 no.3
• /
• pp.146-152
• /
• 2012

MicroRNAs (miRNAs, miRs) are about 21-25 nucleotides in length and regulate mRNA translation by base pairing to partially complementary sites, predominantly in the 3'-untranslated region (3'-UTR) of the target mRNA. In this study, the expression profile of miRNAs was compared and analyzed for the establishment of miRNA-related odontoblast differentiation using MDPC-23 cells derived from mouse dental papilla cells. To determine the expression profile of miRNAs during the differentiation of MDPC-23 cells, we employed miRNA microarray analysis, quantitative real-time PCR (qRT-PCR) and Alizaline red-S staining. In the miRNA microarray analysis, 11 miRNAs were found to be up- or down-regulated more than 3-fold between day 0 (control) and day 5 of MDPC-23 cell differentiation among the 1,769 miRNAs examined. In qRT-PCR analysis, the expression levels of two of these molecules, miR-194 and miR-126, were increased and decreased in the control MDPC-23 cells compared with the MDPC-23 cells at day 5 of differentiation, respectively. Importantly, the overexpression of miR-194 significantly accelerated mineralization compared with the control cultures during the differentiation of MDPC-23 cells. These results suggest that the miR-194 augments MDPC-23 cell differentiation, and potently accelerates the mineralization process. Moreover, these in vitro results show that different miRNAs are deregulated during the differentiation of MDPC-23 cells, suggesting the involvement of these genes in the differentiation and mineralization of odontoblasts.