• Tissue Engineering and Regenerative Medicine
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• 제15권6호
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• pp.735-750
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• 2018

BACKGROUND: The major challenge of tissue engineering is to develop constructions with suitable properties which would mimic the natural extracellular matrix to induce the proliferation and differentiation of cells. Poly(${\varepsilon}$-caprolactone)-poly(ethylene glycol)-poly(${\varepsilon}$-caprolactone) (PCL-PEG-PCL, PCEC), chitosan (CS), nano-silica ($n-SiO_2$) and nano-hydroxyapatite (n-HA) are biomaterials successfully applied for the preparation of 3D structures appropriate for tissue engineering. METHODS: We evaluated the effect of n-HA and $n-SiO_2$ incorporated PCEC-CS nanofibers on physical properties and osteogenic differentiation of human dental pulp stem cells (hDPSCs). Fourier transform infrared spectroscopy, field emission scanning electron microscope, transmission electron microscope, thermogravimetric analysis, contact angle and mechanical test were applied to evaluate the physicochemical properties of nanofibers. Cell adhesion and proliferation of hDPSCs and their osteoblastic differentiation on nanofibers were assessed using MTT assay, DAPI staining, alizarin red S staining, and QRT-PCR assay. RESULTS: All the samples demonstrated bead-less morphologies with an average diameter in the range of 190-260 nm. The mechanical test studies showed that scaffolds incorporated with n-HA had a higher tensile strength than ones incorporated with $n-SiO_2$. While the hydrophilicity of $n-SiO_2$ incorporated PCEC-CS nanofibers was higher than that of samples enriched with n-HA. Cell adhesion and proliferation studies showed that n-HA incorporated nanofibers were slightly superior to $n-SiO_2$ incorporated ones. Alizarin red S staining and QRT-PCR analysis confirmed the osteogenic differentiation of hDPSCs on PCEC-CS nanofibers incorporated with n-HA and $n-SiO_2$. CONCLUSION: Compared to other groups, PCEC-CS nanofibers incorporated with 15 wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications.

• 한국컴퓨터정보학회논문지
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• 제7권4호
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• pp.127-132
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• 2002

본 논문은 수신측이 네트워크 혼잡도를 측정 참여하여 송신측으로 하여금 네트워크 상태에 따른 윈도우 크기를 조절하는데 있다. TCP-RLDM은 기존 TCP 의 Additive Increase/Multiplicative Decrease 방법의 단점을 보완하는 데이터 수신율을 기초로 하는 측정기반 전송 전략을 채택하였다. 이는 유무선 망에서나 지연에 민감하거나 용인하는 응용들로 구성된 이질적 환경에서 동적으로 대응하기 위해 에러손상의 성질에 따른 즉, 혼잡에 의한 손실인지 전송상 일시적 손실인지에 따라서 적절히 대처함으로써 성능을 높일 수 있게 되었다. 수신측으로부터의 데이터 수신율과 에러발생 원인에 대한 정보를 이용, 송신측의 wave 전송방식과 가급적 혼잡이 발생하기 이전에 혼잡회피전략을 적용함으로써 가변적인 네트워크환경에 잘 대처하도록 하였다.

• 한국ITS학회 논문지
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• 제10권2호
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• pp.103-111
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• 2011

최근 메쉬 네트워크는 다양한 분야에서 사용되고 있다. 그러나 메쉬 네트워크는 무선 전송의 특성으로 인한 제한적인 대역폭을 제공하며, 특히 단일 채널로 구성된 메쉬 네트워크의 경우 다중 홉 전송 시 전송률 저하 문제 등이 발생한다. 이러한 문제를 해결하고 QoS(Quality of Service)를 제공하기 위해 다양한 라우팅방식이 제안되어 왔다. 본 논문에서는 MPLS(Multi Protocol Label Switching)를 적용하여 QoS를 제공하는 MPLS 메쉬 네트워크 구조를 제안한다. MPLS를 적용한 메쉬 네트워크를 통해 하부 QoS 제공방식과 무관하게 상위 응용계층에서의 경로관리가 용이해지며 트래픽관리가 용이해진다. 본 논문에서는 IEEE 802.11e 표준을 적용하여 트래픽에 대한 차별적인 서비스를 제공하도록 MPLS 적용 메쉬 라우터를 설계하고, 구현 및 실험을 통하여 동작을 검증하였다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제5권9호
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• pp.1492-1512
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• 2011

Due to multiple hops, mobility and time-varying channel, supporting delay sensitive real-time traffic in wireless local area network-based (WLAN) mesh networks is a challenging task. In particular for real-time traffic subject to medium access control (MAC) layer control overhead, such as preamble, carrier sense waiting time and the random backoff period, the performance of real-time flows will be degraded greatly. In order to support real-time traffic, an efficient adaptive packet scheduling (APS) scheme is proposed, which aims to improve the system performance by guaranteeing inter-class, intra-class service differentiation and adaptively adjusting the packet length. APS classifies incoming packets by the IEEE 802.11e access class and then queued into a suitable buffer queue. APS employs strict priority service discipline for resource allocation among different service classes to achieve inter-class fairness. By estimating the received signal to interference plus noise ratio (SINR) per bit and current link condition, APS is able to calculate the optimized packet length with bi-dimensional markov MAC model to improve system performance. To achieve the fairness of intra-class, APS also takes maximum tolerable packet delay, transmission requests, and average allocation transmission into consideration to allocate transmission opportunity to the corresponding traffic. Detailed simulation results and comparison with IEEE 802.11e enhanced distributed channel access (EDCA) scheme show that the proposed APS scheme is able to effectively provide inter-class and intra-class differentiate services and improve QoS for real-time traffic in terms of throughput, end-to-end delay, packet loss rate and fairness.

• Applied Microscopy
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• 제29권4호
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• pp.511-522
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• 1999

본 연구는 여러 가지 원인으로 인공 유산된 사람 태아 17예를 태령 14주에서 27주로 분류한 뒤, 고환간질내에서 간질세포의 미세구조를 광학현미경과 전자현미경을 이용하여 관찰한 결과 다음과 같은 결론을 얻었다. 1. 사람 태아 고환의 간질세포를 형태적으로 방추형간질세포, 밝은 간질세포, 어두운 간질세포 그리고 변성 간질세포로 분류할 수 있었다. 2. 미성숙한 방추형간질세포는 14주에 많이 관찰되었으며 점차 분화되어 밝은 간질세포와 어두운 간질세포로 변하였다. 3. 밝은 간질세포와 어두운 간질세포는 17주와 24주에 가장 많이 분포하였으며 변성 간질세포는 27주에 많이 관찰되었다. 4. 무과립세포질세망은 밝은 간질세포내에서 현저히 관찰되었으며 점차 내강이 팽대되어 세포질 전반을 차지하고 있었다. 5. 사립체는 밝은 간질세포에 많이 분포하였고 사립체능은 농축되어 진하게 나타났으며, 어두운 간질세포에서 전자밀도가 높은 사립체 함유물을 포함하고 있었다. 6. 지방소적은 태령 14주부터 관찰되어 16주에 가장많은 분포를 보였고 20주 이후 감소하는 양상이 관찰되었다. 7. 간질세포내에 풍부한 양의 당원과립이 관찰되었다. 이상의 결과로 보면 사람 태생기 중 고환의 간질세포는 다른 동물에서와 같이 유사한 분화과정을 거친다고 할 수 있다.

• 전자공학회논문지S
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• 제34S권10호
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• pp.9-25
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• 1997

Personal Communication Networks will be composed of ATM-based broadband ISDN in the future. However, unlike the wired networks, many challenges will arise in the wireless communication service such as PCS. These callenges are frequency alteration of cell routing path, relatively very high error rate at transmission over the wireless interface, etc. Particularly, the alteration of cell routing path caused by handoff makes temporary deterioration of QoS. In this paper, the signaling flows of handoff scenarios which may occur on Personal Communication Networks are presented, and verified by Pertri-Net toolkit. In addition, the cell flow control scheme which minimizes the lagging gap between cells and maintains the cell sequence during handoff is proposed. The proposed scheme can be summarized as the differentiation of normal queue and handoff queue, and the cell flow control between these queues. For verification of the proposed scheme, we used two approaches, which are mathermatical manipulation and SLAM simulation.

• Applied Microscopy
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• 제25권4호
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• pp.17-25
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• 1995

This paper aims to probe the cell differentiation and gene activity in early Oogenesis from Dendrolimus spectabilis Butler by transmission electron microscope. The 8 cystocytes are formed by mitosis of the Oogonia, and differentiated to the 1 Oocyte and 7 nurse cells. The oocyte and nurse cells are connected by ring canals, through which the cytoplasmic organelles such as mitochondria, free ribosomes, and electron dense granular materials are passed from nurse cells to o cyte. Many replication fork in the cystocyte nuclei and 2 transcriptional units of $2.7{\mu}m\;and\;0.36{\mu}m\;or\;0.5{\mu}m$ in the nurse cells are observed by the chromatin spreading technique. It is possible that transcriptional units are passed from nurse cells to Oocyte.

• Molecules and Cells
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• 제42권3호
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• pp.262-269
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• 2019

The porcine myeloid antimicrobial peptide (PMAP), one of the cathelicidin family members, contains small cationic peptides with amphipathic properties. We used a putative lysozyme originated from the bacteriophage P22 (P22 lysozyme) as a fusion partner, which was connected to the N-terminus of the PMAP36 peptide, to markedly increase the expression levels of recombinant PMAP36. The PMAP36-P22 lysozyme fusion protein with high solubility was produced in Escherichia coli. The final purified yield was approximately 1.8 mg/L. The purified PMAP36-P22 lysozyme fusion protein exhibited antimicrobial activity against both Gram-negative and Grampositive bacteria (Staphylococcus aureus, Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, and Bacillus subtilis). Furthermore, we estimated its hemolytic activity against pig erythrocytes as 6% at the high concentration ($128{\mu}M$) of the PMAP36-P22 lysozyme fusion protein. Compared with the PMAP36 peptide (12%), our fusion protein exhibited half of the hemolytic activity. Overall, our recombinant PMAP36-P22 lysozyme fusion protein sustained the antimicrobial activity with the lower hemolytic activity associated with the synthetic PMAP36 peptide. This study suggests that the PMAP36-P22 lysozyme fusion system could be a crucial addition to the plethora of novel antimicrobials.

• Journal of Veterinary Science
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• 제21권2호
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• pp.24.1-24.11
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• 2020

The pandemic of avian influenza viruses (AIVs) in Asia has caused enormous economic loss in poultry industry and human health threat, especially clade 2.3.4.4 H5 and H7 subtypes in recent years. The endemic chicken H6 virus in Taiwan has also brought about human and dog infections. Since wild waterfowls is the major AIV reservoir, it is important to monitor the diversified subtypes in wildfowl flocks in early stage to prevent viral reassortment and transmission. To develop a more efficient and sensitive approach is a key issue in epidemic control. In this study, we integrate multiplex reverse transcription recombinase polymerase amplification (RT-RPA) and capillary electrophoresis (CE) for high-throughput detection and differentiation of AIVs in wild waterfowls in Taiwan. Four viral genes were detected simultaneously, including nucleoprotein (NP) gene of all AIVs, hemagglutinin (HA) gene of clade 2.3.4.4 H5, H6 and H7 subtypes. The detection limit of the developed detection system could achieve as low as one copy number for each of the four viral gene targets. Sixty wild waterfowl field samples were tested and all of the four gene signals were unambiguously identified within 6 h, including the initial sample processing and the final CE data analysis. The results indicated that multiplex RT-RPA combined with CE was an excellent alternative for instant simultaneous AIV detection and subtype differentiation. The high efficiency and sensitivity of the proposed method could greatly assist in wild bird monitoring and epidemic control of poultry.

• Archives of Plastic Surgery
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• 제35권3호
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• pp.229-234
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• 2008

Purpose: There are some reports presenting that peripheral blood contain circulating hematopoietic cells as well as, in significantly smaller quantities, mesenchymal stem cells. The purposes of this study is to isolate and characterize circulating mesenchymal progenitor cells with osteogenic potential from human peripheral blood. Methods: Human buffycoat containing mononuclear cells was harvested from peripheral blood of normal persons and isolated using a density gradient centrifugation and serially subcultured in osteogenic media for 1-4 weeks. The proliferation capability, phase-contrast microscopy, transmission electron microscopy, immunophenotype FACS analysis, Alizarin red staining and RT-PCR assays for osteogenic differentiation potential were performed. Results: The phenotype of cultured cells changed from small round or cuboidal cells at passage 1 into large spindle-shaped fibroblastic morphology cells at passage 4. Surface marker expressed CD14, but did not express CD34, CD80, CD83. Strong positive staining was observed for Alizarin reds in osteogenic medium on day 14, Using RT-PCR, the mRNA levels of bone- specific genes, such as ALP, c-bfa-1 and osteocalcin were detected. Conclusion: A new subset of peripheral blood derived progenitor cells described here has the ability to proliferate and differentiate into osteogenic cell lineages in vitro, and to be candidate for regenerative therapy.