• 가정과삶의질연구
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• 제22권4호
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• pp.43-61
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• 2004

The purpose of this study was to verify the usefulness of kinetic House- Tree- Person drawing as a diagnostic measure for the degree of self-differentiation, which is an essential part of college students' development. Participants for the study were four hundred thirty five(272 male and 163 female) university freshmen enrolled in 4-year colleges located in Pusan. The Participants completed a scale of self-differentiation and a Kinetic House- Tree-Person drawings test. The evaluation system for kinetic House-Tree-Person drawings was established based on the indexes of Buck(1948) and Bums(1972) and used exiting literatures as reference. The data were analyzed using means, standard deviations, t-test, one-way ANOVA, and Scheffe's test. The results obtained from the study are as follows: First, the mean level of college students' self-differentiation was 2.81, which is about average. Some significant gender differences were found in the areas of self-intergration, family projection, and emotional separation. Male students scored higher on self-intergration, whereas female students score higher on family projection and emotional separation. Second. self-differentiation was higher when the student drew a house with smoke coming out of the chimney, a single-story house with flat roof, or with detailed description of curtains, roof and roof tiles. Third, self-differentiation seemed to be higher when branches and fruits were included, when there was no expression of roots, when large crowns and branch openings were presented, and when no slant lines or base lines appeared. Forth, self-differentiation showed ㅁ higher level when the portrait shows eyes, mouth and neck without omission, when it included the whole body instead of face only, and when there was no person with just a profile, a back, or with a stiff posture, and when there was no weak and thin lines. Individuality also marked higher if a person was in motion and than one person was added. Finally, self-differentiation showed significant difference according to the overall harmony of the drawing, ordering of contents, hand pressure, the kind and shape of tree, and presence of other persons. The more harmonious the picture was, and the more family members are added, the higher the level of self-differentiation was.

• Molecules and Cells
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• 제40권6호
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• pp.393-400
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• 2017

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease characterized by lack of insulin and high glucose levels. T2DM can cause bone loss and fracture, thus leading to diabetic osteoporosis. Promoting osteogenic differentiation of osteoblasts may effectively treat diabetic osteoporosis. We previously reported that Sirtuin 1 (Sirt1), a $NAD^+$-dependent deacetylase, promotes osteogenic differentiation through downregulation of peroxisome proliferator-activated receptor (PPAR) ${\gamma}$. We also found that miR-132 regulates osteogenic differentiation by downregulating Sirt1 in a $PPAR{\beta}/{\delta}$-dependent manner. The ligand-activated transcription factor, $PPAR{\alpha}$, is another isotype of the peroxisome proliferator-activated receptor family that helps maintain bone homeostasis and promot bone formation. Whether the regulatory role of $PPAR{\alpha}$ in osteogenic differentiation is mediated via Sirt1 remains unclear. In the present study, we aimed to determine this role and the underlying mechanism by using high glucose (HG) and free fatty acids (FFA) to mimic T2DM in MC3T3-E1 cells. The results showed that HG-FFA significantly inhibited expression of $PPAR{\alpha}$, Sirt1 and osteogenic differentiation, but these effects were markedly reversed by $PPAR{\alpha}$ overexpression. Moreover, siSirt1 attenuated the positive effects of $PPAR{\alpha}$ on osteogenic differentiation, suggesting that $PPAR{\alpha}$ promotes osteogenic differentiation in a Sirt1-dependent manner. Luciferase activity assay confirmed interactions between $PPAR{\alpha}$ and Sirt1. These findings indicate that $PPAR{\alpha}$ promotes osteogenic differentiation via the Sirt1-dependent signaling pathway.

• 동의생리병리학회지
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• 제25권1호
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• pp.29-36
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• 2011

Nardostachyts chinensis (N. chinensis) belonging to the family Valerianaceae has been used to elicit stomachic and sedative effects. The MAPKs are serine/threonine kinases involved in the regulation of various cellular responses, such as cell proliferation, differentiation and apoptosis. The PKC also plays a key role in regulating the response of hematopoietic cells to both physiological and pathological inducers of proliferation and differentiation. This study investigated the signaling pathways on the U937 cell differentiation induced by N. chinensis. N. chinensis induced the differentiation of U937 cells, as shown by increased of differentiation surface antigen CD11b. Activation of ERK increased time-dependently in differentiation of U937 cells induced by N. chinensis, but activations of JNK and p38 were unaffected. Inhibitor of ERK (PD98059) significantly reduced CD11b expression induced by N. chinensis in U937 cells. In addition, N. chinensis increased protein level of PKC ${\beta}$I and PKC ${\beta}$II isoforms, but the protein level of PKC ${\alpha}$ and PKC ${\gamma}$was constant. PKC inhibitors (GF 109203X and H-7) inhibited U937 cell differentiation and the ERK activation induced by N. chinensis. These results indicated that PKC and ERK may be involved in U937 cell differentiation induced by N. chinensis.

• Asian-Australasian Journal of Animal Sciences
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• 제16권8호
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• pp.1199-1204
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• 2003

The aim of this study was to investigate the role of phosphatidylinositol 3-kinase (PI3 kinase) and the mitogenactivating protein (MAP) kinase pathway on the differentiation of ovine preadipocytes. In order to investigate this issue, we monitored glycerol 3-phosphate dehydrogenase (GPDH) activity during differentiation with specific inhibitors of PI3 kinase and MAP kinase-Erk kinase, LY294002 and PD098059, respectively. The preadipocytes, which were obtained from ovine subcutaneous adipose tissues, were proliferated to confluence and then differentiated to adipocytes in differentiation medium with each inhibitor for 10 days. The confluent preadipocytes and differentiated adipocytes at days 3, 7 and 10 were harvested for assay of GPDH activity. LY294002 inhibited the differentiation program in dose- and day-dependent manners during 10 days of differentiation. PD098059 did not affect GPDH activity during differentiation. Furthermore, the expression of peroxisome proliferator-activated receptor ${\gamma}2$ (PPAR-${\gamma}2$), the representative early gene of differentiation, was markedly reduced by LY294002 treatment, although PD098059 did not change it. Our results demonstrated that the activation of PI3 kinase contributes to the differentiation process of ovine preadipocytes.

• 한국수정란이식학회지
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• 제33권2호
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• pp.85-97
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• 2018

The trans-differentiation potential of mesenchymal stem cells (MSCs) is employed, but there is little understanding of the cell source-dependent trans-differentiation potential of MSCs into corneal epithelial cells. In the present study, we induced trans-differentiation of MSCs derived from umbilical cord matrix (UCM-MSCs) and from dental tissue (D-MSCs), and we comparatively evaluated the in vitro trans-differentiation properties of both MSCs into corneal epithelial-like cells. Specific cell surface markers of MSC (CD44, CD73, CD90, and CD105) were detected in both UCM-MSCs and D-MSCs, but MHCII and CD119 were significantly lower (P < 0.05) in UCM-MSCs than in D-MSCs. In UCM-MSCs, not only expression levels of Oct3/4 and Nanog but also proliferation ability were significantly higher (P < 0.05) than in D-MSCs. In vitro differentiation abilities into adipocytes and osteocytes were confirmed for both MSCs. UCM-MSCs and D-MSCs were successfully trans-differentiated into corneal epithelial cells, and expression of lineage-specific markers (Cytokeratin-3, -8, and -12) were confirmed in both MSCs using immunofluorescence staining and qRT-PCR analysis. In particular, the differentiation capacity of UCM-MSCs into corneal epithelial cells was significantly higher (P < 0.05) than that of D-MSCs. In conclusion, UCM-MSCs have higher differentiation potential into corneal epithelial-like cells and have lower expression of CD119 and MHC class II than D-MSCs, which makes them a better source for the treatment of corneal opacity.

• Asian-Australasian Journal of Animal Sciences
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• 제30권7호
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• pp.1029-1036
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• 2017

Objective: In the livestock industry, the regulatory mechanisms of muscle proliferation and differentiation can be applied to improve traits such as growth and meat production. We investigated the regulatory pathway of MyoD and its role in muscle differentiation in quail myoblast cells. Methods: The MyoD gene was mutated by the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 technology and single cell-derived MyoD mutant sublines were identified to investigate the global regulatory mechanism responsible for muscle differentiation. Results: The mutation efficiency was 73.3% in the mixed population, and from this population we were able to establish two QM7 MyoD knockout subline (MyoD KO QM7#4) through single cell pick-up and expansion. In the undifferentiated condition, paired box 7 expression in MyoD KO QM7#4 cells was not significantly different from regular QM7 (rQM7) cells. During differentiation, however, myotube formation was dramatically repressed in MyoD KO QM7#4 cells. Moreover, myogenic differentiation-specific transcripts and proteins were not expressed in MyoD KO QM7#4 cells even after an extended differentiation period. These results indicate that MyoD is critical for muscle differentiation. Furthermore, we analyzed the global regulatory interactions by RNA sequencing during muscle differentiation. Conclusion: With CRISPR/Cas9-mediated genomic editing, single cell-derived sublines with a specific knockout gene can be adapted to various aspects of basic research as well as in functional genomics studies.

• International Journal of Oral Biology
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• 제43권3호
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• pp.133-140
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• 2018

Resveratrol (3,4',5,-trihydroxystilbene), a phytoalexin present in grapes, exerts a variety of actions to reduce superoxides, prevents diabetes mellitus, and inhibits inflammation. Resveratrol acts as a chemo-preventive agent and induces apoptotic cell death in various cancer cells. However, the role of resveratrol in odontoblastic cell differentiation is unclear. In this study, the effect of resveratrol on regulating odontoblast differentiation was examined in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. Resveratrol significantly accelerated mineralization as compared with the control culture in differentiation of MDPC-23 cells. Resveratrol significantly increased expression of ALP mRNA as compared with the control in differentiation of MDPC-23 cells. Resveratrol significantly accelerated expression of Col I mRNA as compared with the control in differentiation of MDPC-23 cells. Resveratrol significantly increased expressions of DSPP and DMP-1 mRNAs as compared with the control in differentiation of MDPC-23 cells. Treatment of resveratrol did not significantly affect cell proliferation in MDPC-23 cells. Results suggest resveratrol facilitates odontoblast differentiation and mineralization in differentiation of MDPC-23 cells, and may have potential properties for development and clinical application of dentin regeneration materials.

• 한국동물생명공학회지
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• 제34권1호
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• pp.2-9
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• 2019

Because mesenchymal stem cells (MSCs) maintain distinct capacities with respect to self-renewal, differentiation ability and immunomodulatory function, they have been highly considered as the therapeutic agents for cell-based clinical application. Of particular, differentiation condition alters characteristics of MSCs, including cellular morphology, expression of gene/protein and cell surface molecule, immunological property and apoptosis. However, the previous results for differentiation-related apoptosis in MSCs have still remained controversial due to varied outcomes. Therefore, the present study aimed to disclose periodical alterations of pro- and anti-apoptosis in MSCs under differentiation inductions. The human dental pulp-derived MSCs (DP-MSCs) were differentiated into adipocytes and osteoblasts during early (1 week), middle (2 weeks) and late (3 weeks) stages, and were investigated on their apoptosis-related changes by Annexin V assay, qRT-PCR and western blotting. The ratio of apoptotic cell population was significantly (p < 0.05) elevated during the early to middle stages of differentiations but recovered up to the similar level of undifferentiated state at the late stage of differentiation. In the expression of mRNA and protein, whereas expressions of pro-apoptosis-related makers (BAX and BAK) were not altered in any kind and duration of differentiation inductions, anti-apoptosis marker (BCL2) was significantly (p < 0.05) elevated even at the early stage of differentiations. The recovery of apoptotic cell population at the late stage of differentiation is expected to be associated with the response by elevation of anti-apoptotic molecules. The present study may contribute on understanding for cellular mechanism in differentiation of MSCs and provide background data in clinical application of MSCs in the animal biotechnology to develop effective and safe therapeutic strategy.

• 한국노년학
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• 제29권4호
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• pp.1629-1644
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• 2009

본 연구는 Bowen 이론을 토대로 노년기 부부가 지각한 자기분화가 정신건강에 미치는 영향에 대해 알아보고자 하였다. 이를 위해 만 55세 이상, 은퇴 이후 배우자와 함께 생활하는 노부부 271쌍(542명)을 대상으로 자료를 수집하였고, 자료분석은 paired t-test, 상관관계, Fisher's r-to-z transformation 및 위계적 중다회귀분석을 실시하였다. 본 연구결과를 요약하면, 첫째 자기분화 수준은 부부간에 차이가 나타나지 않았으나, 하위영역 중 자기입장은 부인보다 남편이 높았고, 정신건강 하위요인 중 우울과 신체화는 남편보다 부인이 높았다. 둘째, 노년기 부부 모두 자기분화 수준은 정신건강의 하위요인과 부적 상관이 있었다. 셋째, 노년기 부부 모두 자기 분화가 정신건강에 영향을 미치는 것으로 자기분화 수준이 높을수록 우울, 불안, 신체화 증상을 낮게 경험하기에 정신건강 수준이 높은 것으로 나타났고 남편의 경우 정서적 반응이, 부인의 경우 정서적 단절이 정신건강 수준에 가장 큰 영향을 미쳤다. 본 연구결과는 Bowen의 자기분화가 노인에게 적용 가능함을 경험적으로 검증했으며, 자기분화가 노년기 부부의 정신건강에 중요한 영향을 미치고 있음을 확인해 주었다. 또한 노년기 부부를 대상으로 정신건강과 자기분화간의 관계 및 인과관계에 관한 연구가 거의 부재한다는 점에서 본 연구의 의의를 찾을 수 있겠다.

• 보건의료산업학회지
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• 제9권3호
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• pp.233-246
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• 2015

Objectives : This study investigated the general characteristics, child rearing characteristics, and the level of self-differentiation of 120 custodial grandmothers. Methods : Data were collected with a self-administered questionnaire from 120 custodial grandmothers who registered Kinship Network in Busan City. Results : For the self-differentiation measure, the mean score of the sample was $2.52{\pm}0.51$. However, the level of the fusion with emotion $1.89{\pm}0.80$ was extremely low partly due to the influence of collectivist culture of Korean society. The levels of emotional reactivity and fusion with emotion were significantly low which were dependent on depression (F=4.387, p=0.015). Conclusions : The findings of this study show the need to improve the level of self-differentiation by increasing the score of emotional reactivity and fusion with the emotion among the kinship network grandmothers. Therefore, supportive programs for kinship network grandmothers need to develop self-differentiation.