International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Resveratrol on Cell Differentiation and Mineralization in Cultured Odontoblasts

  • Shin, Sang Hun (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Kim, Jae-Sung (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Kim, Su-Gwa (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Go, Dae-San (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Yu, Sun-Kyoung (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Kim, Chun Sung (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Park, Joo-Cheol (Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Kim, Do Kyung (Oral Biology Research Institute, School of Dentistry, Chosun University)
  • Received : 2018.07.21
  • Accepted : 2018.08.09
  • Published : 2018.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Resveratrol (3,4',5,-trihydroxystilbene), a phytoalexin present in grapes, exerts a variety of actions to reduce superoxides, prevents diabetes mellitus, and inhibits inflammation. Resveratrol acts as a chemo-preventive agent and induces apoptotic cell death in various cancer cells. However, the role of resveratrol in odontoblastic cell differentiation is unclear. In this study, the effect of resveratrol on regulating odontoblast differentiation was examined in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. Resveratrol significantly accelerated mineralization as compared with the control culture in differentiation of MDPC-23 cells. Resveratrol significantly increased expression of ALP mRNA as compared with the control in differentiation of MDPC-23 cells. Resveratrol significantly accelerated expression of Col I mRNA as compared with the control in differentiation of MDPC-23 cells. Resveratrol significantly increased expressions of DSPP and DMP-1 mRNAs as compared with the control in differentiation of MDPC-23 cells. Treatment of resveratrol did not significantly affect cell proliferation in MDPC-23 cells. Results suggest resveratrol facilitates odontoblast differentiation and mineralization in differentiation of MDPC-23 cells, and may have potential properties for development and clinical application of dentin regeneration materials.

Keywords

References

  1. Lee DS, Yoon WJ, Cho ES, Kim HJ, Gronostajski RM, Cho MI, Park JC. Crosstalk between nuclear factor I-C and transforming growth factor-${\beta}$1 signaling regulates odontoblast differentiation and homeostasis. PLoS One. 2011;6:e29160. doi: 10.1371/journal.pone.0029160.
  2. Mina M1, Kollar EJ. The induction of odontogenesis in non-dental mesenchyme combined with early murine mandibular arch epithelium. Arch Oral Biol. 1987;32: 123-127. https://doi.org/10.1016/0003-9969(87)90055-0
  3. Ruch JV. Odontoblast commitment and differentiation. Biochem Cell Biol. 1998;76:923-938. https://doi.org/10.1139/o99-008
  4. Sun Q, Liu H, Lin H, Yuan G, Zhang L, Chen Z. MicroRNA-338-3p promotes differentiation of mDPC6T into odontoblast-like cells by targeting Runx2. Mol Cell Biochem. 2013;377:143-149. doi: 10.1007/s11010-013-1580-3.
  5. Cho JH, Kim SG, Park BS, Go DS, Park JC, Kim DK. MicroRNA-27 promotes odontoblast differentiation via Wnt1 signaling. Int J Oral Biol. 2015;40:197-204. doi: http://dx.doi.org/10.11620/IJOB.2015.40.4.197.
  6. Tziafas D, Kolokuris I. Inductive influences of demineralized dentin and bone matrix on pulp cells: an approach of secondary dentinogenesis. J Dent Res. 1990;69:75-81. doi: 10.1177/00220345900690011301.
  7. Ritchie HH, Ritchie DG, Wang LH. Six decades of dentinogenesis research. Historical and prospective views on phosphophoryn and dentin sialoprotein. Eur J Oral Sci. 1998;106(Suppl 1):211-220. https://doi.org/10.1111/j.1600-0722.1998.tb02178.x
  8. Telles PD, Hanks CT, Machado MA, Nor JE. Lipoteichoic acid up-regulates VEGF expression in macrophages and pulp cells. J Dent Res. 2003;82:466-470. doi: 10.1177/154405 910308200612.
  9. Tziafas D, Papadimitriou S. Role of exogenous TGF-beta in induction of reparative dentinogenesis in vivo. Eur J Oral Sci. 1998;106(Suppl 1):192-196. https://doi.org/10.1111/j.1600-0722.1998.tb02175.x
  10. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 2000;97:13625-13630. doi: 10.1073/pnas.240309797.
  11. Narayanan K, Srinivas R, Ramachandran A, Hao J, Quinn B, George A. Differentiation of embryonic mesenchymal cells to odontoblast-like cells by overexpression of dentin matrix protein 1. Proc Natl Acad Sci USA. 2001;98:4516-4521. doi: 10.1073/pnas.081075198.
  12. Sreenath T, Thyagarajan T, Hall B, Longenecker G, D'Souza R, Hong S, Wright JT, MacDougall M, Sauk J, Kulkarni AB. Dentin sialophosphoprotein knockout mouse teeth display widened predentin zone and develop defective dentin mineralization similar to human dentinogenesis imperfecta type III. J Biol Chem. 2003;278:24874-24880. doi: 10.1074/jbc.M303908200.
  13. About I, Bottero MJ, de Denato P, Camps J, Franquin JC, Mitsiadis TA. Human dentin production in vitro. Exp Cell Res. 2000;258:33-41. doi: 10.1006/excr.2000.4909.
  14. Choi EJ, Jang SR, Kang OJ, Bang WS. Antimicrobial activity of Psoralea corylifolia, Schisandra chinensis, and Spatholobus suberectus extracts. Kor J Food Sci Technol. 2013;45:495-500. doi: 10.9721/KJFST.2013.45.4.495.
  15. Shin JH, Cha GY, Kim HJ, JH Hwang, Han KH, Seo1 HJ, Shin TS, Oh SJ, Kim JD. Examination of anti-obesity effect of regional special natural products of Anthrisci radix, Psoraleae semen, Siegesbeckiae herba and Corni fructus. KSBB J. 2009;24:549-555.
  16. Vanamala J. Reddivari L. Radhakrishnan S. Tarver C. Resveratrol suppresses IGF-1 induced human colon cancer cell proliferation and elevates apoptosis via suppression of IGF-1R/Wnt and activation of p53 signaling pathways. BMC Cancer. 2010;10:238-251. doi: 10.1186/1471-2407-10-238.
  17. Wang TT, Hudson TS, Wang TC, Remsberg CM, Davies NM, Takahashi Y, Kim YS, Seifried H, Vinyard BT, Perkins SN, Hursting SD. Differential effects of resveratrol on androgen- responsive LNCaP human prostate cancer cells in vitro and in vivo. Carcinogenesis. 2010;29:2001-2010. doi: 10.1002/mnfr.200900143.
  18. Elliott PJ, Jirousek M. Sirtuins: novel targets for metabolic disease. Curr Opin Investig Drugs. 2008;9:371-378.
  19. Jiang H, Shang X, Wu H, Gautam SC, Al-Holou S, Li C, Kuo J, Zhang L, Chopp M. Resveratrol downregulates PI3K/Akt/ mTOR signaling pathways in human U251 glioma cells. J Exp Ther Oncol. 2009;8:25-33.
  20. Karuppagounder SS, Pinto JT, Xu H, Chen HL, Beal MF, Gibson GE. Dietary supplementation with resveratrol reduces plaque pathology in a transgenic model of Alzheimer's disease. Neurochem Int. 2009;54:111-118. doi: 10.1016/j.neuint.2008.10.008.
  21. Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF, Beecher CW, Fong HH, Farnsworth NR, Kinghorn AD, Mehta RG, Moon RC, Pezzuto JM. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science. 1997;275:218-220. https://doi.org/10.1126/science.275.5297.218
  22. Banerjee S, Bueso-Ramos C, Aggarwal BB. Suppression of 7,12- dimethylbenz(a)anthracene-induced mammary carcinogenesis in rats by resveratrol: role of nuclear factor-kappaB, cyclooxygenase 2, and matrix metalloprotease 9. Cancer Res. 2002;62:4945-4954.
  23. Stewart JR, Artime MC, O'Brian CA. Resveratrol: a candidate nutritional substance for prostate cancer prevention. J Nutr. 2003;133:2440-2443. doi: 10.1093/jn/133.7.2440S.
  24. Hope C, Planutis K, Planutiene M, Moyer MP, Johal KS, Woo J, Santoso C, Hanson JA, Holcombe RF. Low concentrations of resveratrol inhibit Wnt signal throughput in colon-derived cells: implications for colon cancer prevention. Mol Nutr Food Res. 2008;52(Suppl 1):52-61. doi: 10.1002/mnfr.200700448.
  25. Hanks CT, Fang D, Sun Z, Edwards CA, Butler WT. Dentin-specific proteins in MDPC-23 cell line. Eur J Oral Sci. 1998;106(Suppl 1):260-266. https://doi.org/10.1111/j.1600-0722.1998.tb02185.x
  26. Hanks CT, Sun ZL, Fang DN, Edwards CA, Wataha JC, Ritchie HH, Butler WT. Cloned 3T6 cell line from CD-1 mouse fetal molar dental papillae. Connect Tissue Res. 1998; 37:233-249. https://doi.org/10.3109/03008209809002442
  27. Sun ZL, Fang DN, Wu XY, Ritchie HH, Begue-Kirn C, Wataha JC, Hanks CT, Butler WT. Expression of dentin sialoprotein (DSP) and other molecular determinants by a new cell line from dental papillae, MDPC-23. Connect Tissue Res. 1998;37:251-261. https://doi.org/10.3109/03008209809002443
  28. Kim DK, Park MG, Lee MH, Yu SK, Park E, Kim S, Lee SA, Moon YH, Kim HJ, Kim CS. MicroRNA analysis during cultured odontoblast differentiation. Int J Oral Biol. 2012; 37:146-152.
  29. Bosshardt DD, Nanci A. Hertwig's epithelial root sheath, enamel matrix proteins, and initiation of cementogenesis in porcine teeth. J Clin Periodontol. 2004;31:184-192. doi: 10.1111/j.0303-6979.2004.00473.x.
  30. Begue-Kirn C, Smith AJ, Loriot M, Kupferle C, Ruch JV, Lesot H. Comparative analysis of TGF beta s, BMPs, IGF1, msxs, fibronectin, osteonectin and bone sialoprotein gene expression during normal and in vitro-induced odontoblast differentiation. Int J Dev Biol. 1994;38:405-420.
  31. Verhaar HJ, Damen CA, Duursma SA, Scheven BA. A comparison of the action of progestins and estrogen on the growth and differentiation of normal adult human osteoblast-like cells in vitro. Bone. 1994;15:307-311. https://doi.org/10.1016/8756-3282(94)90293-3
  32. Kassem M, Mosekilde L, Eriksen EF. Growth hormon stimulates proliferation of normal human bone marrow stromal osteoblast precursor cells in vitro. Growth Regul. 1994;4:131-135.
  33. Choi EM, Suh KS, Kim YS, Choue RW, Koo SJ. Soybean ethanol extract increases the function of osteo-blastic MC3T3-E1 cells. Phytochemistry. 2001;56:733-739. https://doi.org/10.1016/S0031-9422(00)00484-2