• BMB Reports
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• v.55 no.3
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• pp.142-147
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• 2022

Human pluripotent stem cells (PSCs) have been utilized as a promising source in regenerative medicine. However, the risk of teratoma formation that comes with residual undifferentiated PSCs in differentiated cell populations is most concerning in the clinical use of PSC derivatives. Here, we report that a monoclonal antibody (mAb) targeting PSCs could distinguish undifferentiated PSCs, with potential teratoma-forming activity, from differentiated PSC progeny. A panel of hybridomas generated from mouse immunization with H9 human embryonic stem cells (hESCs) was screened for ESC-specific binding using flow cytometry. A novel mAb, K312, was selected considering its high stem cell-binding activity, and this mAb could bind to several human induced pluripotent stem cells and PSC lines. Cell-binding activity of K312 was markedly decreased as hESCs were differentiated into embryoid bodies or by retinoic acid treatment. In addition, a cell population negatively isolated from undifferentiated or differentiated H9 hESCs via K312 targeting showed a significantly reduced expression of pluripotency markers, including Oct4 and Nanog. Furthermore, K312-based depletion of pluripotent cells from differentiated PSC progeny completely prevented teratoma formation. Therefore, our findings suggest that K312 is utilizable in improving stem cell transplantation safety by specifically distinguishing residual undifferentiated PSCs.

• Tuberculosis and Respiratory Diseases
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• v.59 no.6
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• pp.631-637
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• 2005

Background : Transcriptional factors of the CREB(cAMP Response Element Binding Protein) are involved in the regulation of gene expression in response to a variety of signaling pathways. Proteins produced by the CREB genes play key roles in many physiological processes, including memory and long-term potentiation. The retinoic acid receptor (RAR) axis mediates epithelial cell differentiation and proliferation in many tissues including the lung. Material and method : The RAR and CREB expression levels were examined in 60 adenocarcinomas and 60 squamous cell carcinomas of the lung using immunohistochemical staining. Results : 1) RAR protein expression was found in 58.3%(35/60) of adenocarcinomas and 36.7%(22/60) of squamous cell carcinomas(P<0.05). 2) RAR protein expression was found in 80%(16/20) of well differentiated adenocarcinomas, 60%(12/20) of moderately differentiated adenocarcinomas, and 35%(7/20) of poorly differentiated adenocarcinomas (P<0.01). 3) RAR protein expression was found in 45%(9/20) of well differentiated squamous cell carcinomas, 35%(7/20) of moderately differentiated squamous cell carcinomas, and 30%(6/20) of poorly differentiated squamous cell carcinomas (P>0.05). 4) CREB expression was found in 61.7%(37/60) of adenocarcinomas and 40%(24/60) of squamous cell carcinomas( P<0.05). 5) CREB expression was found in 85%(17/20) of well differentiated adenocarcinomas, 60%(12/20) of moderately differentiated adenocarcinomas, and 40%(8/20) of poorly differentiated adenocarcinomas (P<0.01). 6) CREB expression was found in 45%(9/20) of well differentiated squamous cell carcinomas, 35%(7/20) of moderately differentiated squamous cell carcinomas, and 35%(8/20) of poorly differentiated squamous cell carcinomas(P>0.05). 7) RAR and CREB expression was found in 68.5% of lung cancers, and there was a significant correlation between them(P<0.05). Conclusion : RAR and CREB expression can be used to indirectly determine the malignant potentiality of a cell.

• The Journal of Korea Robotics Society
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• v.15 no.2
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• pp.177-183
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• 2020

Collaborative Robot (Cobot) that can collaborate with humans by fusion with many advanced technologies among industrial robots in the industrial field are attracting attention. In this study, the engineers of Small and Medium Enterprises can directly participate in the cobot design, and ultimately, the possibility of deriving the shape design of the differentiated cobot was studied. The method applied to derive the shape design of differentiated cobot is 'Morphological Analysis'. First, the design elements of the form of cobots were derived as 'Link' and 'Joint'. In addition, by analyzing the image form of the Link and Joint of the existing cobot, a new form element of the Link and Joint was proposed. In order to quantitatively identify the most discriminating cobot shape design, FGI (Focus Group Interview) was conducted to derive image types of 4 Link and 3 Joint. Then, the most important 'Shape Combination' was carried out in morphological analysis, and 12 new cobot shape designs were drawn. Through this, the applicability of the morphological analysis method in the derivation of differentiated cobot shape design was examined.

• Proceedings of the KIEE Conference
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• 2006.07d
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• pp.1828-1829
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• 2006

In this paper, we propose sliding mode congestion controller for differentiated-services network. Two important issue in differentiated-services architecture are bandwidth guarantee and fair sharing of unsubscribed bandwidth among TCP flows with and without bandwidth reservation. We use tight upper and lower bounds for various settings of differentiated-services parameters using the loss-bounded model. The Sliding mode congestion controller scheme is designed using nonlinear control theory based on a nonlinear model of the network that is generated using fluid flow consideration. The methodology used is general and independent of technology, as for example TCP/IP or ATM. The sliding mode congestion controller methodology has been applied to an TCP network. We use NS-2 simulation to demonstrate that the proposed control methodology achieves the desired behavior of the network, and possesses important attributes. as e.g, stable and robust behavior, high utilization with bounded delay and loss, together with good steady-state and transient behavior.

• Journal of Communications and Networks
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• v.3 no.4
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• pp.324-333
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• 2001

The Differentiated Services (Diffserv) framework has been proposed by the IETF as a simple service structure that can provide different Quality of Service (QoS) to different classes of packets in IP networks. IP packets are classified into one of a limited number of service classes, and are marked in the packet header for easy classification and differentiated treatments when transferred within a Diffserv domain. The Diffserv framework defines simple and efficient QoS differentiation mechanisms for the Internet. However, the original Diffserv concept does not provide a complete QoS management framework. Since traffic flows in IP networks are unidirectional from one network point to the other and routing paths and traffic demand get dynamically altered, it is important to monitor end-to-end traffic status, as well as traffic status in a single node. This paper suggests a distributed QoS monitoring method that collects the statistical data of each service class in every Diffserv router and calculates edge-to-edge QoS of the aggregated IP flows by combining routing topology and traffic status. A format modeling of edge-to-edge Diffserv flows and algorithms for aggregating edge-to-edge QoS is presented. Also an SNMP-based QoS management prototype system for Diffserv networks is presented, which validates our QoS management framework and demonstrates useful service management functionality.

• Journal of the Institute of Electronics and Information Engineers
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• v.49 no.11
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• pp.205-210
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• 2012

This paper investigated the performance of QoS management using variations of RED parameters in Differentiated Service. Differentiated Service separates packets from a specific traffic by using QoS parameters such as CIR or PIR. A code point is used to mark each packet which is enqueued into each virtual queue. Different RED parameters are configured for virtual queues according to each code point. As the code point value increases, the RED parameters become harsher. To show QoS Management using variations of RED parameters, this paper used the policy model of time sliding window with 2 color marking (TSW2CM). Simulation results using NS-2 showed that the QoS management of a differentiated service can be obtained from using variations of RED parameters.

• Proceedings of the Korea Information Processing Society Conference
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• 2002.11b
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• pp.1591-1594
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• 2002

BE(Best-Effort)방식의 인터넷에서 제공되던 Text위주의 서비스 제공 방식이 Multimedia 위주의 서비스로 점차 바뀌어 감에 따라, 기존의 BE(Best-Effort)방식으로는 Multimedia QoS(Quality of Service)를 보장하기가 어려워졌다. 이에 IETF(Internet Engineering Task Force)에서는 변화하는 인터넷 환경에서 QoS 보장을 위해 Diffserv(Differentiated Service)방식을 제안하게 되었다. 본 논문에서는 Content를 제공하는 Linux Web Server에서 패킷을 클래스 분류기준(Classification Method)에 의해 분류하고 Scheduling Algorithm을 적용하여 DSCP(Differentiated Service CodePoint) 값을 Web Server 자체에서 결정하여 경계라우터(Edge Router)로 전송하는 방식을 취하였으며, 이를 토대로 하여 경계라우터의 Traffic 부하를 줄이고, 경계라우터의 코어라우터(Core Router)화를 통해 더욱더 향상된 Differentiated Service를 제공하는 것이 목적이다. 이를 본 논문에서는 ns2 를 통해 IETF에서 제안된 Diffserv방식과 본 논문에서 제안한 방식의 Diffserv 방식과 현재의 BE방식을 비교하여 어느 정도의 성능 향상이 있었는지 비교 분석하였다.

• Journal of The Korean Association of Information Education
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• v.10 no.3
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• pp.425-434
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• 2006

This study designed and implemented differentiated web courseware for ICT education based on the analysis of the contents of the guidelines for information and communications technology education in elementary schools. The web courseware was designed to provide students with self-directed, differentiated learning in ICT education. The contents of the courseware consist of 'understanding of the computer,' 'mouse and keyboard,' and 'surfing on the Internet.' Each of the contents includes diagnostic and formative evaluations so that students can select their own learning activities of the beginning, intermediate, and advanced levels. The web courseware is expected to be used as self-directed learning materials for ICT education through learner-centered differentiated learning activities.

• The Journal of Asian Finance, Economics and Business
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• v.9 no.5
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• pp.487-496
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• 2022

This study attempts to discover a differentiated service strategy for the hotpot restaurant industry from the perspective of Chinese Generation Z customers, as well as to further explore the inner needs of Chinese Generation Z to make practical implications for discovering the method of gaining their satisfaction and loyalty. This paper employs questionnaires to collect analytical data and through a case study to produce company strategies. Smith's Marketing Ethics Sequential System Model (SMESSM) is introduced in this paper for the decision of whether the case study company Haidilao Hot Pot should make a new strategy on service based on Generation Z's consuming behavior. The findings of this study demonstrate that hotpot restaurant must differentiate their services for Generation Z from older generation customers to gain a sustainable development of the hotpot business. Proper differentiated service will not only improve Generation Z's dining experience but also reduce costs. This paper is the first to discuss differentiated service strategy in the hotpot restaurant business from the perspective of Generation Z customers. And a Chinese experience of SMESSM for practical use is introduced in this paper, which enriches the relevant implications for future research on business strategy.

• Korean Journal of Head & Neck Oncology
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• v.20 no.1
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• pp.7-12
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• 2004

Background and Objectives: Brain metastasis from well-differentiated thyroid carcinoma is uncommon, and the treatment remains controversial. In this study, we analyzed cases of brain metastasis from well differentiated thyroid carcinoma to determine optimal treatment. Material and Methods: From March 1986 to May 2003, we experienced 13 cases of brain metastases from well-differentiated thyroid carcinomas. There were 6 men and 7 women with a mean age of 62.3 years. The time interval detecting the brain metastasis, treatment methods, outcomes, and the prognostic factors were analyzed. Results: In 11 patients, brain metastasis was found at a mean of 96 months after thyroid surgery (range, 1-204months). In 1 patient, brain metastasis was found simultaneously with the thyroid cancer. In the remaining one, brain metastasis was presented as the first sign. Treatment included gamma knife therapy in 2, external radiation therapy (ExRT) in 2, resection and ExRT in 2, gamma knife therapy and ExRT in 2 and intracranial holmium injection in 1. Three patients received no treatment. The mean survival was 20.5months (1-119 months). Only 2 patients with early detection remained alive after treatment (23months and 119months). The age, sex, primary tumor size, combined organs of distant metastasis and treatment methods did not affect the prognosis. There were noticeable symptomatic improvements in patients who received any kind of treatment for brain metastasis. Conclusion: Early detection and treatment of brain metastasis appears to bring symptomatic relief and improve survival. Therefore, early screening for brain metastasis is recommended for patients with symptoms. However, further study is needed to determine the optimal method of treatment.