Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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Immunohistochemical Expression of Nuclear Retinoid Receptor and CREB(cAMP Response Element Binding Protein) in Lung Cancers

폐암종에서 Nuclear Retinoid Receptor 및 CREB의 면역조직화학적 발현 양상

  • Shin, Jong Wook (Department of internal Medicine, Chung-Ang University) ;
  • Gi, Seung-Seok (Department of Pathology, Chung-Ang University) ;
  • Paik, Kwang Hyun (Department of Internal Medicine, Korea Cancer Center Hospital) ;
  • Choi, Won (Department of Internal Medicine, Korea Cancer Center Hospital) ;
  • Park, In Won (Department of internal Medicine, Chung-Ang University) ;
  • Kim, Mi Kyung (Department of Pathology, Chung-Ang University)
  • 신종욱 (중앙대학교 의과대학 내과학교실) ;
  • 기승석 (중앙대학교 의과대학 병리학교실) ;
  • 백광현 (원자력병원 내과) ;
  • 최원 (원자력병원 내과) ;
  • 박인원 (중앙대학교 의과대학 내과학교실) ;
  • 김미경 (중앙대학교 의과대학 병리학교실)
  • Received : 2005.09.13
  • Accepted : 2005.10.05
  • Published : 2005.12.30
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Abstract

Background : Transcriptional factors of the CREB(cAMP Response Element Binding Protein) are involved in the regulation of gene expression in response to a variety of signaling pathways. Proteins produced by the CREB genes play key roles in many physiological processes, including memory and long-term potentiation. The retinoic acid receptor (RAR) axis mediates epithelial cell differentiation and proliferation in many tissues including the lung. Material and method : The RAR and CREB expression levels were examined in 60 adenocarcinomas and 60 squamous cell carcinomas of the lung using immunohistochemical staining. Results : 1) RAR protein expression was found in 58.3%(35/60) of adenocarcinomas and 36.7%(22/60) of squamous cell carcinomas(P<0.05). 2) RAR protein expression was found in 80%(16/20) of well differentiated adenocarcinomas, 60%(12/20) of moderately differentiated adenocarcinomas, and 35%(7/20) of poorly differentiated adenocarcinomas (P<0.01). 3) RAR protein expression was found in 45%(9/20) of well differentiated squamous cell carcinomas, 35%(7/20) of moderately differentiated squamous cell carcinomas, and 30%(6/20) of poorly differentiated squamous cell carcinomas (P>0.05). 4) CREB expression was found in 61.7%(37/60) of adenocarcinomas and 40%(24/60) of squamous cell carcinomas( P<0.05). 5) CREB expression was found in 85%(17/20) of well differentiated adenocarcinomas, 60%(12/20) of moderately differentiated adenocarcinomas, and 40%(8/20) of poorly differentiated adenocarcinomas (P<0.01). 6) CREB expression was found in 45%(9/20) of well differentiated squamous cell carcinomas, 35%(7/20) of moderately differentiated squamous cell carcinomas, and 35%(8/20) of poorly differentiated squamous cell carcinomas(P>0.05). 7) RAR and CREB expression was found in 68.5% of lung cancers, and there was a significant correlation between them(P<0.05). Conclusion : RAR and CREB expression can be used to indirectly determine the malignant potentiality of a cell.

배 경 : 폐를 포함한 인체내 여러 조직에서 상피세포의 분화 및 증식에 중요한 역할을 담당한다고 알려진 Retinoid acid(RA)와 여러 유전자들에서 전사조절인자로 성장관여 유전자들의 활성화에 관여하며 세포증식 및 분화에 매우 중요한 세포내 조절인자인 cAMP response-element binding protein(CREB)의 폐암종에서의 발현정도를 알아보고 조직학적 차이에 따른 발현도를 비교분석하여 발암과정에서의 관여여부와 역할을 파악하고자 하였다. 방 법 : 중앙대학교 의과대학 부속병원에서 최근 10년간 시행한 기관지내시경 및 흉부외과적 적출을 통해 얻어진 폐암종 조직중 파라핀 포매의 보관상태가 양호한 120예(선암종 60예, 편평세포암종 60예)를 연구대상으로 면역조직화학적 염색을 시행하였다. 결 과 : RAR과 CREB 모두 편평세포암종에 비해 선암종에서 발현이 의의있게 높았고(P<0.05) 선암종에서는 조직학적으로 분화도가 좋을수록 높은 발현율을 보였다(P<0.01). 총 120예의 폐암종에서 RAR과 CREB의 발현을 비교하면 65.8%의 동시발현율을 나타냈다(P<0.05) 결 론 : RAR과 CREB은 폐조직에서 점액상피세포의 분화와 상관관계가 있으며 편평세포암종보다는 선암종의 발암과정에서 일부 의미있는 역할을 수행하리라 생각되었다. 또한 RAR과 CREB의 발현부위도 통계적으로 의미 있는 일치양상을 나타내어 이들은 서로 상호작용에 의해 발암과정 중 일부 역할을 수행하리라 생각된다.

Keywords

References

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