• Asian-Australasian Journal of Animal Sciences
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• 제25권1호
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• pp.98-103
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• 2012

This study was conducted to re-evaluate the dietary vitamin C requirement in juvenile eel, Anguilla japonica by using L-ascorbyl-2-monophosphate (AMP) as the vitamin C source. Five semi-purified experimental diets were formulated to contain 0 ($AMP_0$), 30 ($AMP_{24}$), 60 ($AMP_{52}$), 120 ($AMP_{108}$) and 1,200 ($AMP_{1137}$) mg AMP $kg^{-1}$ diet on a dry matter basis. Casein and defatted fish meal were used as the main protein sources in the semi-purified experimental diets. After a 4-week conditioning period, fish initially averaging $15{\pm}0.3$ g (mean${\pm}$SD) were randomly distributed to each aquarium as triplicate groups of 20 fish each. One of five experimental diets was fed on a DM basis to fish in three randomly selected aquaria, at a rate of 3% of total body weight, twice a day. At the end of the feeding trial, weight gain (WG) and specific growth rate (SGR) for fish fed $AMP_{52}$ and $AMP_{108}$ were significantly higher than those recorded for fish fed the control diet (p<0.05). Similarly, feed efficiency (FE) and protein efficiency ratio (PER) for fish fed $AMP_{52}$ were significantly higher than those for fish fed the control diet (p<0.05). Broken-line regression analysis on the basis of WG, SGR, FE and PER showed dietary vitamin C requirements of juvenile eel to be 41.1, 41.2, 43.9 and 43.1 (mg $kg^{-1}$ diet), respectively. These results indicated that the dietary vitamin C requirement could range from 41.1 to 43.9 mg $kg^{-1}$ diet in juvenile eel when L-ascorbyl-2-monophosphate was used as the dietary source of vitamin C.

• 대한지역사회영양학회지
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• 제22권4호
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• pp.307-322
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• 2017

Objectives: This study examined the effects of nutrition education focused on personalized daily energy requirement and food units using Food Exchange System on anthropometric, biochemical characteristics, nutrition knowledge, dietary attitude and nutrient intakes for overweight and obese in a public health center. Methods: The subjects were 60 overweight/obese women based on BMI (educated 30 vs. non-educated 30, 50~64 years). Educated group was provided individual and/or group lessons (40 min/lesson/week, 5 week), 'Introduction: obese & health', '6 nutrients and 6 food groups', 'My obesity & daily needed energy', 'Meal planning for personalized daily energy and food units using Food Exchange Systems', and 'Smart food choices'. After education, we examined the differences in anthropometric/biochemical characteristics, nutrition knowledge, dietary attitude and nutrient intakes between educated group and non-educated group. Results: After nutrition education, in the educated group, there were improvements on anthropometric/biochemical characteristics, nutrition knowledge, dietary attitude and nutrient intakes in the educated group compared to the non-educated group. We observed a decrease in the mean weight, total cholesterol (TC) and the incidence of overweight/obesity and hypercholesterolemia and an increase in the mean lean body mass. The scores of nutrition knowledge, 'Function of carbohydrate, protein, vitamin, mineral' and 'Food Sources of fat, vitamin, mineral' were increased. The scores of dietary attitudes, 'Taking a joyful meal, a leisurely meal, a balanced meal, a meal with sufficient vegetables, a meal with diversity, a meal with spicy foods, a meal with overeating' were increased. The intakes of energy, carbohydrate, fat, protein, vitamin A, thiamin, Zn and cholesterol were decreased. The scores of INQ, protein, vitamin A, vitamin C, thiamin, riboflavin, vitamin B6, folate, Ca, P, Fe, Zn were increased. Conclusions: The nutrition education focused on personalized daily energy requirement and food exchange unit using Food Exchange System for overweight and obese may improve food behavior, dietary intakes and symptoms of overweight and obese, even in a community health center.

• 한국가정과교육학회지
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• 제24권2호
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• pp.51-62
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• 2012

본 연구는 1962년 한국인 영양권장량의 제정부터 2010년 한국인 영양섭취기준 개정에 이르기까지 성인과 임신 수유부의 단백질의 섭취권장량 산출방법과 섭취권장량의 변화 추이를 살펴보았다. 1. 성인의 단백질 섭취권장량은 1989년까지 요인가산법을, 그 이후에는 질소균형연구를 활용하여 설정되었다. 요인가산법을 적용하였던 시기는 표준단백질 최소 필요량 또는 불가피 질소손실량을 기본 요인으로 하고 식이단백질의 이용율을 적용하였으며, 질소균형연구를 적용하였던 시기는 질소평형 유지에 필요한 식이단백질의 최소 필요량을 기본 요인으로 하였다. 그리고 개인 차, 스트레스 등의 조정 요인을 반영하여 단백질 섭취권장량을 계산하였다. 단백질 섭취권장량은 남성 50~80g/일, 여성 45~70g/일이었으며, 남성의 섭취권장량이 여성보다 크고, 연령대가 높을수록 섭취권장량은 감소하였다. 2. 임신부의 단백질 부가섭취권장량은 태아의 발육에 기인하는 단백질 축적량을 기본 요인으로 하고 식이단백질 이용율 등의 조정요인을 적용하여 산출하였고, 10~30g/일이었으며, 2010년에는 임신 기간을 3분하여 각각 0, 15, 30g/일을 제시하였다. 3. 수유부의 단백질 부가섭취권장량은 모유로 분비되는 단백질의 양을 기본 요인으로 하고 식이단백질 이용율 등의 조정요인을 적용하여 산출하였고, 20~30g/일이었으며, 시기별 증감 경향은 나타나지 않았다. 앞으로 체중 대신 제지방체중을 적용하는 단백질 섭취권장량의 산출 공식이 마련될 필요성이 있으며, 에너지와 마찬가지로 섭취하는 식이단백질의 조성에 근거한 개인별 섭취권장량의 계산방법 고안을 제언한다. 그리고 수유부의 경우 수유 기간을 구분하여 기간별 단백질 부가섭취권장량의 제정을 제언한다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권8호
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• pp.1186-1194
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• 2009

The objective of this study was to estimate the optimum dietary essential amino acid pattern for male Taiwan country chicks. A series of experiments was conducted with chicks, 14 d of age, for 2 wks. A basal synthetic diet was established using a dose response test for all essential amino acids referring to the broiler requirements recommended by NRC (1994). Twelve chicks were sacrificed at the beginning to provide initial body nitrogen data, and every group of six birds received the basal diet or a diet with a deficiency in a single essential amino acid in twenty one treatments by intubation according to their daily metabolic body weight (MBW). Deposited body nitrogen was determined using comparative slaughtering. According to the daily intake from the limiting amino acid per unit of MBW and the body nitrogen accretion rates for every two deficient groups with the same limiting amino acid but at different levels, a corresponding straight line was computed for each essential amino acid to intersect with a horizontal line made by the body nitrogen accretion rate of the control group. The x coordinate of the intersection represented the daily requirement for growth plus maintenance based on MBW corresponding to the essential amino acid. The amino acid ratios can be considered as the optimum pattern of dietary essential amino acids. The results, expressed with respect to lysine = 100, were arginine 105, methionine 81, histidine 34, tryptophan 18, leucine 103, phenylalanine 135, isoleucine 69, threonine 65 and valine 79. This pattern could be utilized to compute the dietary requirements (g/kg feed) for all essential amino acids by multiplying by the requirement of a single essential amino acid cited from the literature.

• Nutrition Research and Practice
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• 제16권sup1호
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• pp.11-20
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• 2022

Research articles were reviewed to validate the estimated energy requirements (EERs) equations developed by the Institute of Medicine of the National Academies (IOM). These equations are based on total energy expenditure (TEE) measured by the doubly labeled water (DLW) method. We subsequently aimed to provide the basis for the suitability to apply the IOM equations as EER equations for Koreans, and develop relevant equations for EER in the Dietary Reference Intake for Koreans (KDRI). Additionally, besides the EER(IOM) equations, other equations were examined for EER estimation. Research papers demonstrating the validation of the EER(IOM) equations based on TEE(DLW) were searched through PubMed (up to September 2019). Of the 637 potentially relevant articles identified, duplicates and unsuitable titles and abstracts were excluded. Furthermore, papers with irrelevant subject and inappropriate study design were also excluded. Finally, 11 papers were included in the review. Among the reviewed papers, 8 papers validated the application of the EER(IOM) equations for EER based on TEE(DLW). These included 3 studies for children (USA 1, Korea 2), 1 for adolescents (Portugal), 2 for adults (Korean), and 2 for the elderly (Korea, USA). EER(IOM) equations were found to be generally acceptable for determining EER by using the DLW method, except for Korean boys at 9-11 yrs (overestimated) and female athletes at 19-24 yrs (underestimated). Additionally, 5 papers include the validation of other EER equations, beside EER(IOM) for EER based on TEE(DLW). In Japanese dietary reference intake and recommended dietary allowance, EER equations are acceptable for determining EER based on TEE(DLW). The EER(IOM) equations is generally acceptable for determining EER using the DLW method in Koreans as well as several populations, although certain defined groups were found to be unfit for the estimation. Additionally, the concept of healthy body mass index of Koreans and physical activity levels need to be considered, thereby providing the basis for developing relevant equations of EER in KDRI.

• Journal of Nutrition and Health
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• 제30권10호
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• pp.1160-1169
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• 1997

The purpose of this research was to investigate the effects of caffeine and calcium levels on calcium utilization in female rats of different ages. Calcium utilization was compared in female rats of different age( 4 weeks and 12 months) fed various levels of caffeine(0 and 7 mg/100g body weight) and calcium (50, 100 and 200% of requirement) for 3 weeks. Feed intake of the caffeine groups were lower than that of the no-caffeine groups. body weight gain was lowest in the high-caffeine and low-calcium group. Serum calcium levels of young rats were higher than those of adult rats. There were no significant differences in tibial calcium content among the caffeine and calcium -groups. Fecal calcium excretion increased as the level of dietary calcium was increased. Urinary calcium excretion increased as the levels of caffeine and dietary calcium were increased. With increasing levels of dietary calcium , daily calcium retention was accelerated, but apparent calcium absorbability was diminished. The results of this study suggest that caffeine consumption promotes urinary calcium excretion. However, increase in dietary calcium resulted in higher calcium retention . These findings indicate that high caffeine consumption may increase dietary calcium requirements. Therefore, it could be suggested that the supplementation of dietary calcium may counteract the negative effect of caffeine intake on calcium utilization.

• 한국가금학회지
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• 제15권3호
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• pp.229-246
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• 1988

본 시험은 하절기 산란계의 생산성 저하 방지 및 사료효율개선을 목적으로 하절기 산란계 사료의 적정 에너지수준 및 단백질수준을 구명하기 위해 대사에너지 3수준(2,500, 2,700, 2,900kcal/kg) 과 단백질 2수준(15%, 17%)을 조합한 6개 처리에 49주령 된 산란기 480수를 공시하여 1988년 6월 10일부터 1988년 9월 1일까지 12주간에 걸쳐 시험을 실시하였던 바 그 결과를 요약하면 다음과 같다. 1. 생존율은 전처리구에서 98.75~100%로 처리간에 유의성이 없었으며, 대사에너지수준이나 단백질수준간에도 일정한 경향이나 큰 차이가 없었다. 2. 산란율, 난중 및 산란량은 대사에너지 2,500 kcal/kg, 단백질 17%수준에서 각각 84.37%, 62.29g, 그리고 52.55g으로 가장 우수했으며 대사에너지수준이 증가함으로써 점차 낮아졌고, 사료 내 단백질수준이 증가함으로써 유의하게 높아졌으나(p＜0.05), 산란율은 대사에너지 2,500kcal/kg과 2,700kcal/kg, 난중은 2,500kcal/kg과 2,700kcal/kg 및 2,700kcal/kg과 2,900kcal/kg 수준간에는 유의한 차이가 없었다. 3. 사료 섭취량은 대사에너지 2,500kcal/kg, 단백질 55%수준에서 118.28g/day으로 가장 높았으며, 대사에너지수준이 증가함에 따라 사료 섭취량은 유의하게 감소했으나(p＜0.05), 사료중 단백질 수준에 따라서는 큰 차이가 없었다. 4. 대사에너지 섭취량은 대사에너지 2,900kcal/kg, 단백질 15%수준에서 326.60kcal/day로 가장 높았으며 대사에너지수준이 증가함에 따라 유의하게 증가되었으나(p＜0.05), 사료중 단백질 수준에 따라서는 유의한 차이가 없었다. 5. 단백질섭취양은 대사에너지 2,500kcal/kg, 단백질 17%수준에서 19.99 g/day로 가장 높았으며 대사에너지수준이 증가함에 따라 유의하게 감소하였으나, 사료 중 단백질수준이 증가함에 따라 유의하게 증가하였다(p＜0.05). 6 사료 요구율은 대사에너지와 2,500kcal/kg과 단백질 17%수준에서 2.24로 가장 낮았으나, 사료중 대사에너지수준에 따라서는 큰 차이가 없었으나, 단백질수준이 증가함에 따라 유의하게 개선되었다 (p＜0.05). 7. 산란kg당 대사에너지와 단백질 요구량은 2,900kcal/kg와 15% 및 2,700kcal/kg와 17%의 대사에너지와 단백질수준에서 각각 6871.64kcal와 385.76g으로 가장 높았으며 사료중의 에너지수준이 증가할수록 산란kg당 대사에너지요구량은 유의하게 증가하였으나(p＜0.05), 단백노요구량을 일정한 경향을 보이지 않았고. 사료중 단백질수준이 증가함에 따라 산란당 대사에너지 요구량은 유의하게 감소한 반면 산란당 단백질요구량은 유의하게 증가하였다(p＜0.05). 산란kg당 사료비는 2,500kcal/kg과 15%의 대사에너지와 단백질수준에서 279.84 won으로 가장 적었다. 이상의 결과를 종합하여 볼 때, 우리나라 실정에 맞는 하절기 산란계사료의 적정 대사에너지 및 단백질수준은 각각 2,500kcal/kg과 17%가 가장 적합할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제9권5호
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• pp.551-556
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• 1996

Selenium (Se) apparent absorption and retention in sheep as influenced by diets differing in protein content through soybean meal supplementation was studied. A $3{\times}3$ Latin square design was used with three Japanese Corriedale wethers (45 kg average body weight), three periods, and three dietary treatments. In each period, 7 d dietary adjustment was followed by 5 d total collection of urine and feces. The three dietary treatments were : Diet 1, without soybean meal supplementation (14% crude protein, CP); Diet 2, with 10% soybean meal supplementation (16.5% CP); and Diet 3, with 20% soybean meal supplementation (19% CP). All the diets had a Se supplementation in the form of sodium selenite at 0.2 mg Se/kg dietary DM. The dietary DM intake of the animals was 2% of their body weight. No significant differences were obtained among the three dietary treatments of the Se balance of the animals. However, as percent of Se intake, only urinary Se concentration of Diet 3 was markedly lower (p < 0.05) than the other diets. Fecal Se as percent of Se intake followed the trend of Diet 3> Diet 2 > Diet 1 resulting a Se absorbed as percent of Se intake of 58.9%, 62.3% and 68.2% for Diets 3, 2 and 1, respectively but their differences among each other were insignificant. No significant differences that were observed either on Se retained as percent of intake (Diet 1, 48.2%; Diet 2, 45.2%; Diet 3, 46.0%) or Se retained as percent of Se absorbed (Diet 1, 70.7%; Diet 2, 72.4%; Diet 3, 77.9%). Significant correlation coefficients among the various measures of Se utilization were also observed. Regression analysis showed the following equation: Y = 93.8 - 1.86X (p <0.05, $r^{2}=0.48$), where Y is the Se absorbed as percent of Se intake (%) and X is the dietary protein content (%). This study concludes that Se requirement in sheep is greater when dietary protein content is high.

• Nutrition Research and Practice
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• 제17권1호
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• pp.48-61
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• 2023

BACKGROUND/OBJECTIVES: Magnesium is an essential nutrient for human health. However, inadequate intake is commonly reported worldwide. Along with reduced consumption of vegetables and fruits and increased consumption of refined or processed foods, inadequate magnesium intake is increasingly reported as a serious problem. This study aimed to assess magnesium intake, its dietary sources, and the adequacy of magnesium intake in Korean populations. SUBJECTS/METHODS: Data was obtained from the Korea National Health and Nutrition Examination Survey 2016-2019 and included individuals aged ≥1 yr who had participated in a nutrition survey (n=28,418). Dietary intake was assessed by 24-h recall, and dietary magnesium intake was estimated using a newly established magnesium database. Diet adequacy was evaluated by comparing dietary intake with the estimated average requirement (EAR) suggested in the Korean Dietary Reference Intakes 2020. RESULTS: The mean dietary magnesium intake of Koreans aged ≥1 yr was 300.4 mg/d, which was equivalent to 119.8% of the EAR. The prevalence of individuals whose magnesium intake met the EAR was 56.8%. Inadequate intake was observed more in females, adolescents and young adults aged 12-29 yrs, elders aged ≥65 yrs, and individuals with low income. About four-fifths of the daily magnesium came from plant-based foods, and the major food groups contributing to magnesium intake were grains (28.3%), vegetables (17.6%), and meats (8.4%). The top 5 individual foods that contributed to magnesium intake were rice, Baechu (Korean cabbage) kimchi, tofu, pork, and milk. However, the contribution of plant foods and individual contributing food items differed slightly by sex and age groups. CONCLUSIONS: This study found that the mean dietary magnesium intake among Koreans was above the recommended intake, whereas nearly one in 2 Koreans had inadequate magnesium intake. To better understand the status of magnesium intake, further research is required, which includes the intake of dietary supplements.

• Journal of Nutrition and Health
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• 제32권6호
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• pp.654-660
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• 1999

Folate, a precursor of the coenzyme tetrahydrofolate, plays an important role in DNA replication and cell proliferation, and thus could influence rapidly proliferating immune cells such as leukocytes and splenocytes. The effects of dietary folate on folate concentrations of plasma, thymus, spleen and leukocytes were investigated in rats. The animals were raised for 6 weeks on semipurifed experimental diets containing 0mg, 2mg, 4mg, 8mg folate/kg diet. Folate concentrations were determined microbiologically using Lactobacillus casei(ATCC 7469), and DNA strand breaks produced by alkaline treatment were analyzed fluorometrically. When compared to folate adequate diet, the folate deficient diet(0mg folate/kg diet) resulted in lowest folate levels in plasma, thymus, spleen and leukocytes, and the highest DNA strand breaks in spleen cells and leukocytes. Dietary folate levels significantly increased folate concentrations of immune tissues, leukocytes, and the plasma in a dose dependent manner, folate concentrations being highest with a diet providing 8mg folate/kg diet. The percentages of the double strand DNA remaining in the splenocytes and leukocytes after alkaline treatment were significantly increased with higher amounts of dietary folate in a dose dependent manner. Folate intakes of 8mg than 4mg/kg diet was found to be more effective in the prevention of DNA strand breaks. The results of this study suggest that increased folate above the requirement level could improve DNA stabilities in immune cells.