• The Journal of Pediatrics of Korean Medicine
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• v.20 no.2
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• pp.177-196
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• 2006

Objective : This experimental study was designed to determine the effects of BPT on obesity in vivo and in vitro. Methods : in vitro, BPTn extracts of various concentration(50, 100, 200 ${\mu}g/m{\ell}$) were added in 3T3-L1 cell. Adipocyte differentiation was measured by Oil Red O staining and Morphological examination. The expression of $C/EBP{\alpha}$ and $PPAR{\gamma}$ receptor was measured by western blot assay and RT-PCR in vivo, Rats were orally administered BPT daily for consecutive four weeks before poloxamer-407 induced hyperlipidemic state. The rats were sacrificed 24 hrs later for poloxamer-407 treated and then serum triglyceride, total cholesterol were measured ; Rats were orally administered BPT daily for consecutive four weeks before triton WR-1339 induced hyperlipidemic state. The rats were sacrificed 40 hrs later for triton WR-1339 treated and then serum triglyceride, total cholesterol were measured ; Rats with obesity were induced by the high fat-diet for six weeks and then serum triglyceride, total cholesterol, LDL-cholesterol, triglyceride, HDL-cholesterol, hydroxy radical, superoxide dismuatse activity were measured. Results : In vitro, The 3T3-L1 cells' differentiation was significantly decreased by BPT. The expression of $C/EBP{\alpha}$ and $C/EBP{\beta}$ was decreased by BPT. In vivo, BPT significantly reduced serum triglyceride, total cholesterol contents in poloxamer-407 treated rat. BPT significantly reduced serum triglyceride contents in Triton WR-1339 treated rat. Total cholesterol also reduced but did not show a significant change. BPT significantly reduced body weight gain of rat and adipose tissue mass of rats and serum triglyceride, LDL-cholesterol contents and significantly increased HDL-cholesterol, HTR(HDL-cholesterol/Total-cholesterol) in rats with obesity induced by the high fat-diet. BPT reduced blood lipid peroxide, hydroxy radical and increased superoxide dismuatse(SOD) activity.

• Korean Journal of Pharmacognosy
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• v.32 no.2 s.125
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• pp.145-152
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• 2001

Whangryunhaedok-Tang (WT) is formulated with Coptidis Rhizoma, Phellodendri Cortex, Scutellariae Radix and Gardeniae Fructus, and Daewhang-whangryunhaedok-Tang (DWT) is made by the combination of Rhei Rhizoma, a wellknown anticostipation drug in WT. Therefore, DWT has been evaluated for antihyperlipidemic effects on experimental hyperlipidemic rats and mice induced by corn oil and high cholesterol-diet. Oral administration of DWT significantly inhibited the increase of serum triglyceride and LDL-cholesterol levels, and the decrease of serum HDL-cholesterol levels in hyperlipidemic rats induced by corn oil. Also, oral administration of DWT significantly prevented the increase of serum total cholesterol, triglyceride and LDL-cholesterol, and liver total cholesterol and triglyceride in 1% cholesterol-diet fed mice. These results suggest that DWT is effective for the treatment of hyperlipidemia.

• Korean Journal of Pharmacognosy
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• v.25 no.3
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• pp.264-271
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• 1994

Pharmacological effects of water extracts of Lycii Fructus, Lycii Folium and Lycii Cortex Radicis from Lycium chinensis were investigated. Lycii Folium significantly protected the hepatic function from damages orally caused by $CCl_4 $administration in mice and had a strong hypoglycemic effect in hyperglycemic mice induced by streptozotocin. Lycii Fructus decreased the blood pressure rise associated with the growth of normal rats. Lycii Cortex Radicis had a strong hypoglycemic effect in hyrerglycemic mice induced by streptozotocin. Also, hypolipidemic effects in hyperlipidemic rats induced by 1% cholesterol fed-diet and 75% fructose were significantly observed by oral administration of water extracts of Lycii Fructus, Lycii Folium and Lycii Cortex Radicis.

• Korean Journal of Acupuncture
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• v.27 no.1
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• pp.107-123
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• 2010

Objective & Methods: The purpose of this study is to investigate the effects of Artemisiae Capillaris Herba herbal-acupuncture (ACH-HA) at GB34 (Yangleungchean) on hyperlipidemia induced with alloxan injection and high fat diet in rats. The author performed several experimental items to analyze the levels of various components and enzymes in serum, liver, as well as the histological changes of liver and aorta. Results: 1. ACH-HA solution increased the DPPH radical scavenging activity in rat liver cells. 2. ACH-HA at GB34 significantly decreased the levels of serum total cholesterol, low-density lipoprotein (LDL)-cholesterol, free cholesterol and atherogenic index (AI), while significantly increased the ratios of high-density lipoprotein (HDL)/total cholesterol and phospholipid/total cholesterol in hyperlipidemic rats. 3. ACH-HA at GB34 significantly decreased serum aspartate aminotransferase (AST) level in hyperlipidemic rats. 4. ACH-HA at GB34 significantly increased hepatic glutathione (GSH) activity in hyperlipidemic rats. Conclusion: From the above results, it is suggested that ACH-HA at GB34 may have therapeutic and preventive effects on hyperlipidemia.

• Journal of Pharmaceutical Investigation
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• v.26 no.3
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• pp.215-219
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• 1996

Active component was sought among three herb medicines, which are used in combination as a traditional medicine prescribed for patients with hyperlipidemia related diseases. Antihyperlipidemic effect of this remedy has previously been shown model by the authors on the animal model. Hyperlipidemia was induced on male Wistar rats by keeping them on high lipid diet for one week, as previously described by the authors. Blood lipid profile was verified on these rats by measuring total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL). Then, the diet was changed to normal. At the same time, methanol extracts of Scutellaris baicalensis Georg.(radix), Coptidis japonica Makino(rhizoma) or Rhei koreanum Nakai(rhizoma) were given on daily basis, and changes in the blood lipid profile were monitored for 4 weeks. Methanol extract of Scutellaris baicalensis Georg. significantly reduced the TC value, implying the in vivo antihyperlipidemic effect.

• The Journal of Internal Korean Medicine
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• v.35 no.2
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• pp.119-132
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• 2014

Objectives : This study was undertaken to investigate the effects of Samulhwalhyeol-tang (SM) in a hyperlipidemic animal model induced by a high-fat diet using diverse biological methods. Methods : This study was to determine whether fractionated EtOH extracts of SM inhibit reactive oxygen species (ROS) and nitric oxide (NO) in RAW 264.7 cells. Hyperlipidemia was induced by a high-fat diet fed for 6 weeks. Total cholesterol, LDL cholesterol, HDL cholesterol, triglyceride, glucose, liver function, cholesterol gene revelation control efficiency, and histologic change of liver were measured after oral administration of SM. Results : 1. Compared to the control group, ROS production (%) and NO production (%) were decreased significantly by SM. 2. Total cholesterol, LDL cholesterol, triglyceride were decreased significantly by SM. 3. HDL cholesterol was increased significantly by SM. 4. Rats' body weight and glucose were decreased significantly by SM. 5. AST, ALP were decreased significantly by SM. 6. In histopathologic examination, fatty liver and fiver fibrosis were inhibited, almost eliminated as round of fat was observed in the liver. Conclusions : SM would appear that effective in the prevention and treatment of atherosclerosis, ischemic heart disease, other cardiovascular diseases, fatty liver and nephrotic syndrome caused by hyperlipidemia.

• Korean Journal of Acupuncture
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• v.25 no.4
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• pp.73-88
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• 2008

Objective : The aim of this research was to investigate the effect on five element constitutional acupuncture(Sa-am acupuncture) at 4 acupoints by needle manipulation to sedate Six Fu-Organ on hyperlipidemia induced by high fat diet in rats. Method : We recorded data of weight gain, food intake, food efficiency, weight of liver, heart, spleen, lung, kidney, levels of total cholesterol, triglyceride, HDL-cholesterol, AST, ALT in serum of rats fed high fat diet for 5 weeks. Spraque Dawley rats were divided into eight groups(normal; normal diet and non-acupuncture group, control; non-acupuncture group, Acu-LI; acupuncture at $SI_5{\cdot}LI_5{\cdot}BL_{66}{\cdot}LI_2$ group, Acu-ST; acupuncture at $GB_{41}{\cdot}ST_{43}{\cdot}LI_1{\cdot}ST_{45}$, Acu-SI; acupuncture at $BL_{66}{\cdot}SI_2{\cdot}ST_{36}{\cdot}SI_8$, Acu-BL; acupuncture therapy at $ST_{36}{\cdot}BL_{40}{\cdot}GB_{41}{\cdot}BL_{65}$, Acu-TE; acupuncture at $BL_{66}{\cdot}TE_2{\cdot}ST_{36}{\cdot}TE_{10}$, Acu-GB; acupuncture at $LI_1{\cdot}GB_{44}{\cdot}SI_5{\cdot}GB_{38}$. After needles inserted, it was rotated clockwise and anticlockwise 12 times on rats fed high fat diet. Results : The body weight was decreased in Acu-SI, Acu-TE, Acu-GB group, /the food intake was decreased in Acu-TE, / the food efficiency was decreased in Acu-SI, /serum triglyceride was decreased in Acu-LI, Acu-ST, Acu-BL, Acu-GB, /serum total cholesterol was decreased in Acu-LI, Acu-BL, serum ALP was decreased in Acu-LI, Acu-ST, Acu-BL, Acu-TE, Acu-GB. While the relative weight of heart was increased in Acu-LI, Acu-SI, the relative weight of kidney was increased in Acu-SI, Acu-GB. Conclusion : These results suggest that five element constitutional acupuncture(Sa-am acupuncture) may be having an therapeutic influence on hyperlipidemia induced by high fat diet. Further researches of acupuncture manipulation are needed in the future based on our study.

• Mycobiology
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• v.46 no.2
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• pp.159-167
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• 2018

This study evaluated the in vitro and in vivo hypolipidemic effects of the medicinal mushroom Phellinus pini. The methanol extract (ME) of the fruiting body of Ph. pini was active against pancreatic lipase and cholesterol esterase with 99.14% and 67.23% inhibited activity at 1.0 mg/mL, respectively. It also inhibited 81.81% and 55.33% of ${\alpha}$-glucosidase and ${\alpha}$-amylase activities, respectively, at 2.0 mg/mL. Hyperlipidemia as induced by feeding rats with a high fat and cholesterol diet (HFC). HFC supplemented with a 5% fruiting body powder of Ph. pini (HFC + PhP) significantly reduced plasma total cholesterol, low-density lipoprotein cholesterol, and triglycerides in rats compared with HFC. The reduced levels were comparable to rats fed the normal control diet (NC). The atherogenic index of HFC + PhP rats was significantly lower than that of the HFC rats. The excretion of fecal total lipid and cholesterol in the HFC + PhP rats was significantly higher than those in the NC and HFC rats. Histopathological examinations demonstrated scant deposition of lipids in the liver of rats fed HFC + PhP. The dietary supplementation with the fruiting body powder provided natural plasma lipid and glucose lowering effects in experimental rats without adverse effects on the plasma biochemical parameters and liver function related enzyme activities. Therefore, the hypolipidemic effects of Ph. pini may be due to the inhibitory effects on pancreatic lipase, cholesterol esterase, ${\alpha}$-glucosidase, and ${\alpha}$-amylase, and excretion of excess lipids and cholesterol in the feces.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.1
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• pp.23-30
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• 1994

This study was carried out to investigate the effect of the feeding mixture of linseed oil, rich in n-3 PUFA and the sunflower seed oil, rich in n-6 PUFA on the lipid metabolism in the dietary hyperlipidemic rats. After male Sprague-Dawley rats were induced hyperlipidemia by feeding the diet containing lard, butter and cholesterol for 3 weeks, then they were fed with the diet containing lard 3.0 % and butter 12.0% for control, the mixture in different proportion of both linseed oil and sunflower seed oil and antihyperlipidemic drugs for 2 weeks. Analysis of the lipid component of the serum showed following results. Concentration of total cholesterol in serum was significantly lower in the other groups except group 2 (lard 3.0 % + olive oil 12.0%) than in the control group , especially in groups 3 (cholestyramine 2.0%) and 5 (lard 3.0% + linseed oil 12.0%). HDL-C and the ratio of HDL-C to total cholesterol concentration were higher in groups 1, 3, 5 and 9 (lard 3.0% + sunflower seed oil 12.0%) , while the atherosclerotic index was low in groups3, 5. Concentrations of free cholesterol and choesteryl ester in serum were significantly lower in the other groups except group 2 than in the control group, especially lower in grop 5. Concentration of LDL was significantly higher in group 2 while it was remarkably lower in groups 3 and 5, while those of triglyceride in groups in 3 and 4 (lipraroid) were significantly higher than in the control groups. From the data on concentration of total choelsterol , HDL-cholesterol, LDL, phospholipid and triglyceride in serum, the results suggested that the feeding mixed with 3.0% lard and 12.0% linseed oil or 2.0% cholestyramine were most effective for the improvement of the serum lipids.

• Journal of Pharmaceutical Investigation
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• v.29 no.1
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• pp.55-60
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• 1999

We previously showed that a methanol extract of Paeoniae radix decreased total cholesterol level in rats with hyperlipidemia. In order to isolate the active ingredient(s), the methanol extract of Paeoniae radix was fractionated with chloroform/methanol(4:1) solution and isolate into soluble part and insoluble part of the the methanol extract. Above two parts were tested on the experimentally induced hypercholesterolemia in rats for the lowering effect of serum lipoprotein contents. Hyperlipidemia was induced on male Wistar rats by feeding high choleserol diet for 7 days. After oral administration of above samples for 4 weeks, serum lipid profile was verified on these rats by measuring total cholesterol, triglyceride, high density lipoprotein cholesterol and low density lipoprotein cholesterol. The chloroform/methanol(4:1) soluble part and insolule part showed lowering activity of total cholesterol level and triglyceride level at 4 week point significantly(p<0.01 and p<0.05) compare with the control group and the soluble part was more effective.