Journal of Pharmaceutical Investigation
- Volume 29 Issue 1
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- Pages.55-60
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- 1999
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- 2093-5552(pISSN)
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- 2093-6214(eISSN)
Isolation of Hyperlipidemic Substances from Methanol Extract of Paeoniae Radix
작약의 메탄올 추출물로부터 항고지혈 활성성분의 분리
- Ro, Hwan-Seong (University of Ulsan, College of Medicine) ;
- Ko, Woo-Kyoung (Asan Institute of Life Science) ;
- Yang, Hyun-Ok (Asan Institute of Life Science) ;
- Park, Kun-Ku (Asan Institute of Life Science) ;
- Cho, Young-Hwan (Department of Pharmacy, Asan Medical Center) ;
- Lee, Yong-Eon (College of Medicine, Dong A University) ;
- Park, Hyung-Sup (University of Ulsan, College of Medicine)
- 노환성 (울산대학교의과대학) ;
- 고우경 (아산생명과학연구소) ;
- 양현옥 (아산생명과학연구소) ;
- 박건구 (아산생명과학연구소) ;
- 조영환 (아산재단서울중앙병원약제부) ;
- 이용언 (동아대학교 의과대학) ;
- 박형섭 (울산대학교의과대학)
- Published : 1999.03.20
Abstract
We previously showed that a methanol extract of Paeoniae radix decreased total cholesterol level in rats with hyperlipidemia. In order to isolate the active ingredient(s), the methanol extract of Paeoniae radix was fractionated with chloroform/methanol(4:1) solution and isolate into soluble part and insoluble part of the the methanol extract. Above two parts were tested on the experimentally induced hypercholesterolemia in rats for the lowering effect of serum lipoprotein contents. Hyperlipidemia was induced on male Wistar rats by feeding high choleserol diet for 7 days. After oral administration of above samples for 4 weeks, serum lipid profile was verified on these rats by measuring total cholesterol, triglyceride, high density lipoprotein cholesterol and low density lipoprotein cholesterol. The chloroform/methanol(4:1) soluble part and insolule part showed lowering activity of total cholesterol level and triglyceride level at 4 week point significantly(p<0.01 and p<0.05) compare with the control group and the soluble part was more effective.