• 대한상한금궤의학회지
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• v.10 no.1
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• pp.37-45
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• 2018

Objective : The purpose of this paper is to report the improvement of a patient who had a postpartum disease treated with herb medication based on Shanghanlun provisions. Methods : According to 'Disease Pattern Identification Diagnostic System based on Shanghanlun Provisions', the patient was diagnosed with TaeYang-byung, number 12 provision, and was administered herb medication for 4 months. Visual Analogue Scale(VAS) was used to estimate the patient's status. Results : The VAS changed 10 to 0. These results suggest that cold pain and weakness in the legs have improved. Conclusions : The patient had an experience of miscarriage 3 times and finally got a baby but with postpartum disease. She treated with the herb medication for 4 months and the outcome was found effective and economical due to the diagnostic system based on Shanghanlun provisions.

• Journal of Korean Medical classics
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• v.22 no.4
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• pp.241-247
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• 2009

Body constitution research in Korean traditional medicine adopt the diagnosis and treatment theory of "image-differentiation[body constitution differentiation, disease differentiation] in combination with syndrome differentiation diagnostic model and symptoms and signs of herbal property belong to image" as its core, which is key national medical science research project of State Administration of Traditional Chinese Medicine, the project brought up 4 key scientific problem ? body constitution differentiation theory, correlation theory of body constitution and disease, body constitution adjustable theory and symptoms and signs of herbal property belong to image theory. In body constitution pathology, it brought up "correlation between body constitution and symptoms", "differentiation between body constitution and symptoms" which increase the diagnostic level and diagnostic accuracy rate. In the condition of pathology, it obviously has low reliability according to body constitution differentiation, sometimes happen the description not comply with body constitution and disease symptoms, which lead to decrease the clinic diagnostic and treatment level, treatment effect not satisfying too. Now taking 4 key scientific achievement as criterion to illustrate the body constitution and disease symptoms.

• Korean Journal of Veterinary Research
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• v.58 no.3
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• pp.125-129
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• 2018

We investigated the prevalence of canine brucellosis in companion and stray dogs between March 2015 and December 2016 and determined the disease characteristics based on the geographic distribution, sex and age of the dogs in South Korea. We conducted a large-scale survey using serological and bacteriological tests. Samples were collected from 2,394 dogs (1,825 companions and 569 strays). Thirty (1.3%) samples were positive for Brucella canis antibodies including 16 (0.9%) from companion dogs and 14 (2.5%) from stray dogs. Two (1.0%) of the 196 samples cultured from the stray dogs were positive. When compared with male dogs, the female companion and stray dogs had a significantly higher prevalence of brucellosis. Moreover, the prevalence of canine brucellosis was significantly higher in stray dogs older than 6 years and the prevalence of the disease in companion dogs was highest in Incheon (2.1%) and Jeolla (2.1%) provinces. Stray dogs from the Daejeon metropolitan area had the highest prevalence of brucellosis (7.9%). National control measures for canine brucellosis have not previously been implemented. Our findings suggest that appropriate screening tests and control measures are necessary to improve the health of dogs and to protect public health in Korea, particularly with the rapid growth of the companion animal industry.

• Korean Journal of Veterinary Service
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• v.42 no.4
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• pp.275-278
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• 2019

Blindness was observed in five calves born from cattle fed only a commercial feed of growing stage and dried rice straws for about two years in a farm in Gyeongsangbuk-do province. Three of them died within a month after birth, and a body and sera of his mother and other 19 cattle were submitted for diagnosis. At necropsy, the calf was very weak and filled with cerebrospinal fluid in the cerebrum. Any histopathological lesion including atrophy of death of optic nerve cells was not observed, but the irregular proliferation such as lace pattern of choroidal cells and lymphocytic infiltration just below choroid was observed. No pathogen was detected as a result of the etiological tests on the internal organs of calves and bloods. In addition, the levels of serum vitamin A in different affected and his mother cattle were all lower than normal. Finally, we determined this case as an encephalopathy caused by maternal vitamin A deficiency in a calf. This report is an extreme example of how important it is to supply adequate s diets and a good quality of hay for each stage of growth in cattle.

• Korean Journal of Veterinary Service
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• v.41 no.1
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• pp.41-45
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• 2018

An 11 year-old male Korean short-haired cat was presented to local animal hospital due to weight loss, vomiting, and intestinal hypomotility. After the cat was euthanized by poor clinical outcomes, necropsy was performed at Animal and Plant Quarantine Agency. At necropsy, the stomach was enlarged and had some nearly complete pellet food and the yellow mucous contents. The lumen of the middle and lower parts of the jejunum became narrow. Histopathologically, medium-sized lymphoid cells with hyperchromatic nuclei enclosed by scant cytoplasm were diffusely proliferated from mucosa to serosa of the small intestine. These findings were mainly observed in the jejunum and slightly in the duodenum and ileum. The monomorphous lymphocytes were 1 to 1.5 times larger than red blood cells and had few mitotic figures. Metastasis of the tumor cells to other organs was not observed. In the result of immunohistochemical analysis for identifying the origin of tumor cells, CD3 was expressed, but $CD79{\alpha}$ was not detected in the infiltrated cells. This case was diagnosed as T cell intestinal lymphoma in a Korean short-haired cat based on the clinical signs, gross findings, histopathology, and immunohistochemistry.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.24 no.1
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• pp.1-13
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• 2020

Objectives Mibyeong is an ideological concept that means the state between the healthy and diseased conditions. The purpose of this study was to suggest a research direction to establish the diagnostic criteria for the Mibyeong by reviewing the research results for the Mibyeong. Methods Academic databases (OASIS for Korean database, Embase for English database, and CNKI for Chinese database) were used to search related literatures, and articles describing the concept or diagnostic criteria of the disease were selected. Results The concept of Mibyeong consisted of three different conditions: (1) subjective symptoms without a specific disease, (2) abnormal examination findings without a specific disease, and (3) a state in between health and disease. No matter which of the three conditions is applied, the spectrum of condition was very wide and diverse. Conclusions It is impossible to apply appropriate and monolithic diagnostic criteria to all types of Mibyeong. Therefore, we suggests that the Mibyeong be classified into several subtypes and the diagnostic criteria suitable for each type be established.

• Korean Journal of Veterinary Service
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• v.39 no.2
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• pp.107-116
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• 2016

In the study, we developed and evaluated a uracil N-glycosylase (UNG)-supplemented single-tube nested reverse transcription-polymerase chain reaction (UsnRT-PCR) assay that can carried out first-round RT-PCR and second-round nested PCR in a reaction tube without reaction tube opening and can simultaneously detect EU- and NA-PRRSV. The UsnRT-PCR confirmed to have a preventing ability of mis-amplification by contamination of pre-amplified PRRSV DNA from previous UsnRT-PCR. Primer specificities were evaluated with RNAs extracted from 8 viral strains and our results revealed that the primers had a high specificity for both genotypes of PRRSV. The sensitivity of the UsnRT-PCR was 0.1 $TCID_{50}$/0.1 mL for EU- or NA-PRRSV, respectively, which is comparable to that of previously reported real time RT-PCR (RRT-PCR). Clinical evaluation on 110 field samples (60 sera and 50 lung tissues) by the UsnRT-PCR and the RRT-PCR showed that detection rates of the UsnRT-PCR was 70% (77/110), and was relatively higher than that of the RRT-PCR (69.1%, 76/110). The percent positive or negative agreement of the UsnRT-PCR compared to RRT-PCR was 96.1% (73/76) or 90.9% (30/33), showing that the test results of both assays may be different for some clinical samples. Therefore, it is recommend that diagnostic laboratory workers use the two diagnostic assays for the correct diagnosis for the relevant samples in the swine disease diagnostic laboratories. In conclusion, the UsnRT-PCR assay can be applied for the rapid, and reliable diagnosis of PRRSV without concerns about preamplified DNA carryover contamination that can occurred in PCR process in the swine disease diagnostic laboratories.

• Tuberculosis and Respiratory Diseases
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• v.77 no.6
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• pp.271-273
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• 2014

Disease flare-up after discontinuing epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) has been considered as a critical issue in lung cancer patients who have experienced radiologic progression after showing initial durable response. This is a case of systemic nocardiosis that occurred after chronic steroid use for radionecrosis from stereotactic radiosurgery. It was initially thought as a disease flare-up after stopping EGFR-TKI.

• 대한상한금궤의학회지
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• v.8 no.1
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• pp.109-119
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• 2016

Objective : The purpose of this paper is to report the effect of Daehamhyung-tang(DHHT) on tinnitus with panic disorder from the disease pattern identification diagnostic system based on Shanghanlun provisions. Methods : According to a disease pattern identification diagnostic system based on Shanghanlun provisions, the patient was diagnosed with 'Taeyang-byung Gyeolhyung'. He took DHHT for 82 days. This paper evaluated the results of treatment on tinnitus by Tinnitus Handicap Inventory(THI) and Verbal Numercial Rating Scale(VNRS). To assess the mental state of anxiety, this paper used Hamilton Anxiety Rating Scale(HAM-A) Results : The THI score was declined from 58 to 6 And the VNRS score was declined from 6 to 1. HAM-A score was declined from 28 to 3. Conclusions : This case study suggests that DHHT has effectiveness on a patient suffers from tinnitus with panic disorder and diagnosed with 'Taeyang-byung Gyeolhyung'.

• Journal of Veterinary Science
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• v.23 no.2
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• pp.21.1-21.7
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• 2022

Newcastle disease (ND), infectious laryngotracheitis (ILT) and avian metapneumovirus (aMPV) can be similar making it critical to quickly differentiate them. Herein, we adapted pre-existing molecular-based diagnostic assays for NDV and ILTV, and developed new assays for aMPV A and B, for use under synchronized thermocycling conditions. All assays performed equivalently with linearity over a 5 log₁₀ dynamic range, a reproducible (R² > 0.99) limit of detection of ≥ 10 target copies, and amplification efficiencies between 86.8%-98.2%. Using biological specimens for NDV and ILTV showed 100% specificity. Identical amplification conditions will simplify procedures for detection in diagnostic laboratories.