Browse > Article
http://dx.doi.org/10.4142/jvs.21153

Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry  

Mo, Jongseo (Poultry Diagnostic and Research Center, Department of Population Health, College of Veterinary Medicine, University of Georgia)
Angelichio, Michael (IDEXX Laboratories, Inc.)
Gow, Lisa (IDEXX Laboratories, Inc.)
Leathers, Valerie (IDEXX Laboratories, Inc.)
Jackwood, Mark W. (Poultry Diagnostic and Research Center, Department of Population Health, College of Veterinary Medicine, University of Georgia)
Publication Information
Journal of Veterinary Science / v.23, no.2, 2022 , pp. 21.1-21.7 More about this Journal
Abstract
Newcastle disease (ND), infectious laryngotracheitis (ILT) and avian metapneumovirus (aMPV) can be similar making it critical to quickly differentiate them. Herein, we adapted pre-existing molecular-based diagnostic assays for NDV and ILTV, and developed new assays for aMPV A and B, for use under synchronized thermocycling conditions. All assays performed equivalently with linearity over a 5 log10 dynamic range, a reproducible (R2 > 0.99) limit of detection of ≥ 10 target copies, and amplification efficiencies between 86.8%-98.2%. Using biological specimens for NDV and ILTV showed 100% specificity. Identical amplification conditions will simplify procedures for detection in diagnostic laboratories.
Keywords
Newcastle disease virus; avian infectious laryngotracheitis virus; avian metapneumovirus; quantitative real-time PCR;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Villegas P. Viral diseases of the respiratory system. Poult Sci. 1998;77(8):1143-1145.   DOI
2 Alexander DJ. Avian Paramyxoviridae--recent developments. Vet Microbiol. 1990;23(1-4):103-114.   DOI
3 Shin HJ, Njenga MK, McComb B, Halvorson DA, Nagaraja KV. Avian pneumovirus (APV) RNA from wild and sentinel birds in the United States has genetic homology with RNA from APV isolates from domestic turkeys. J Clin Microbiol. 2000;38(11):4282-4284.   DOI
4 Van de Zande S, Nauwynck H, Cavanagh D, Pensaert M. Infections and reinfections with avian pneumovirus subtype A and B on Belgian turkey farms and relation to respiratory problems. Zentralbl Veterinarmed B. 1998;45(10):621-626.
5 Mo J, Angelichio M, Gow L, Leathers V, Jackwood MW. Validation of specific quantitative real-time RTPCR assay panel for infectious bronchitis using synthetic DNA standards and clinical specimens. J Virol Methods. 2020;276:113773.   DOI
6 Kim LM, Suarez DL, Afonso CL. Detection of a broad range of class I and II Newcastle disease viruses using a multiplex real-time reverse transcription polymerase chain reaction assay. J Vet Diagn Invest. 2008;20(4):414-425.   DOI
7 Moriya Y, Nakamura T, Okamura N, Sakaeda T, Horinouchi M, Tamura T, et al. Comparison of synthetic DNA templates with authentic cDNA templates in terms of quantification by real-time quantitative reverse transcription polymerase chain reaction. Biol Pharm Bull. 2006;29(3):535-538.   DOI
8 Kalendar R, Khassenov B, Ramankulov Y, Samuilova O, Ivanov KI. FastPCR: an in silico tool for fast primer and probe design and advanced sequence analysis. Genomics. 2017;109(3-4):312-319.   DOI
9 Svec D, Tichopad A, Novosadova V, Pfaffl MW, Kubista M. How good is a PCR efficiency estimate: recommendations for precise and robust qPCR efficiency assessments. Biomol Detect Quantif. 2015;3:9-16.   DOI
10 Bagust TJ, Jones RC, Guy JS. Avian infectious laryngotracheitis. Rev Sci Tech. 2000;19(2):483-492.   DOI
11 Dimitrov KM, Abolnik C, Afonso CL, Albina E, Bahl J, Berg M, et al. Updated unified phylogenetic classification system and revised nomenclature for Newcastle disease virus. Infect Genet Evol. 2019;74:103917.   DOI
12 Dimitrov KM, Ramey AM, Qiu X, Bahl J, Afonso CL. Temporal, geographic, and host distribution of avian paramyxovirus 1 (Newcastle disease virus). Infect Genet Evol. 2016;39:22-34.   DOI
13 Naylor C, Shaw K, Britton P, Cavanagh D. Appearance of type B avian Pneumovirus in great Britain. Avian Pathol. 1997;26(2):327-338.   DOI
14 Kaleta EF, Baldauf C. Newcastle disease in free-living and pet birds. In: Alexander DJ, editor. Newcastle Disease. Boston: Springer; 1988, 197-246.
15 Callison SA, Riblet SM, Oldoni I, Sun S, Zavala G, Williams S, et al. Development and validation of a real-time Taqman PCR assay for the detection and quantitation of infectious laryngotracheitis virus in poultry. J Virol Methods. 2007;139(1):31-38.   DOI
16 Hoorfar J, Malorny B, Abdulmawjood A, Cook N, Wagner M, Fach P. Practical considerations in design of internal amplification controls for diagnostic PCR assays. J Clin Microbiol. 2004;42(5):1863-1868.   DOI