• 한국해양환경ㆍ에너지학회지
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• 제19권4호
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• pp.341-348
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• 2016

미세조류는 대체에너지를 위한 생물자원 중 하나로 다양한 분야에서 관련된 연구가 활발히 진행되고 있다. 미세조류의 상태를 분석하기 위한 방법으로는 계수법, 스크리닝법, 응집법 등이 사용되고 있는데, 이 중 응집법은 적조 제거제 연구, 미세조류 자원화 연구 등에 효과적으로 이용되고 있다. 미세조류의 응집 상태 분석에는 현재 분광광도법이 주로 사용되고 있는데, 이는 미세조류의 응집 상태를 광학밀도 계측을 통해 분석하는 방법으로 미세조류 계수법에 비해 소요되는 분석 시간은 작지만, 측정 결과의 오차가 상대적으로 큰 단점을 갖고 있다. 본 논문에서는 이러한 단점을 개선하기 위해서 렌즈프리 그림자 이미징 기술을 이용하여 미세조류의 응집 현상을 실시간으로 분석하는 방법을 제안한다. 본 연구에서는 렌즈프리 그림자 이미지를 이용하여 단일 미세조류의 측정과 응집 미세조류 측정이 동시에 가능함을 현미경 이미지와의 비교를 통해 입증하였다. 또한, 해당 기술을 기반으로 미세조류의 응집 현상을 정량적으로 분석할 수 있는 세 가지의 그림자 파라미터(플록들의 개수, 플록들의 유효면적 및 최대크기 플록의 면적)를 제안하였다. 각 파라미터의 유효성은 응집 효율이 다른 응집제를 이용한 실험을 통해 시간에 따른 미세조류의 응집상태를 실시간으로 분석하여 입증할 수 있음을 확인하였다.

• 자원환경지질
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• 제21권1호
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• pp.1-15
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• 1988

This is a study on the mineral compositions, SK numbers of refractoriness and the genesis of the clay mineral deposits in Cheonnam Province and Handong area. 1. Jindo kaolin deposits: Chief clay minerals of the deposits are kaolinite, quartz and alunite. The SK number of the ore is from $34^+$(the highest) to 27(the lowest). On the genesis of the deposits some geologists believe that the deposits were formed by the alteration of the siliceous tuff. But the deposits seems to be formed by the hydrothermal alteration of the rhyolite lava beds. This area is formed by alternative beds of tuff; and kaoline deposits. 2. Hadong area: Chief mineralogy of Hadong kaolin area is $10{\AA}$ halloysite and kaolinite. The SK number of some of the ore is up to $36^+$. The theoretic SK number of kaolinitic composition is 35. So one of the highest alumina minerals of gibbsite is formed in the ores of $36^+$ SK numbers. 3. Hampyong kaolin deposits: Most of kaolin has black color. The chief minerals are kaolinite, quartz and muscovite. Some of the kaoline contains rutile crystals. SK number ranges from 30 to 17. The kaolin deposit is formed by the transported sedimentation in lower part of the seashore. 4. Jangsan kaoline deposits: Chief minerals of the kaolin is kaolinite, quartz and muscovite. Some kaoline contains small crystals of pyrite. This area consists almost of the tuffs. Kaolin deposits also would be formed by the alteration of the tuffs. 5. Nohwado pyrophyllite deposits: Quartz and pyrophyllite are chief minerals. SK number of the ore ranges from 32 to 30. The pyrophyllite deposits would be formed by the hydrothermal alteration of the rhyolitic lava beds. This area consists of alterative beds of tuffs and rhyolitic lavas. 6. Songsuk pyrophyllite deposits: Chief minerals are quartz, kaolinite, pyrophyllite and iron oxides. In the pyrophyllite deposits egg-like inclusions of diaspore and kaolinite in composition. This area almost consists of tuffs. Several faults are developed and along the fault the tuff would begin to alter to pyrophyllite and some parts to diaspore and kaolinite nodules by the acts of hydrothermal solution.

• 자원환경지질
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• 제29권5호
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• pp.545-559
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• 1996

Rare metal (Nb-Zr-REE) ore deposits are located in the Chungju area. Geotectonically, the rare metal ore deposits are situated in the transitional zone between Kyeonggi massif and Okcheon belt. The rare metal deposits are distributed in Kyemyeongsan Formation which consist of schist and alkaline igneous rocks. Alkali granite has suffered extensive post-magmatic metasomatism and hydrothermal processes. The ore contains mainly Ce-La, Ta-Nb, Y, Y-Nd, Nd-Th group minerals. More than 15 RE and REE minerals are found in the ore deposits. Allanite, one of the Ce-La rich REE minerals belonging to the epidote group, is the most common mineral in the studied area. The allanite- bearing rocks may be devided into seven types by features of occurrence and mineral associations; zircon type (ZT), allanite-vein type (AT), feldspar type (KT), fluorite type (FT), quartz-mica type (QT), iron-oxide type (MT), and amphibole type (HT). The allanite veins (AT) and zircon rich rocks (ZT) contain the highest total REE contents. Differences in REE abundance can be interpreted in terms of varying portions of magmatic hydrothermal fluid. Petrographical and chemical data are presented for allanites which were collected from different types. The allanites show wide variations in optical properties, due in part to differences in their chemical composition (depending on the types) and to the degree of crystallinity of the individual specimens. Allanite metamicts in biotite are generally surrounded by well developed pleochroic haloes. Usually, allanite is accompanied by zircon and other REE-bearing minerals. CaO and total REE contents $({\sum}RE_2O_3)$ range from 9.29 to 18.79% and 11.66 to 26.31%, respectively. Also, SiO, (28.87~32.61%), $Al_2O_3$ (8.30~16.88%), and $Fc_2O_3$ (16.74~24.38%) contents show varying contents from type to type. The ${\sum}RE_2O_3$ of allanite has positive relationships with $Fe_2O_3$ and negative relaton with CaO, $SiO_2$, and $Al_2O_3$ Backscattered electron microscope images (BEl) of allanite shows that the its mineral composition and texture is very complex. The allanite-bearing hosts show distinct light REE enrichment with strong negative Eu anomaly except for HI. The HT has an almost flat REE distribution pattern with a small negative Eu anomaly. The chemical variation of the allanites with occurrences and mineral association can be related to condition of temperature and oxidation states in precipitation environment.

• 한국해안·해양공학회논문집
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• 제27권5호
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• pp.324-338
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• 2015

진행파 혹은 임의 반사율을 갖는 부분중복파 혹은 완전중복파-흐름-해저지반의 상호작용에 관한 해석해가 Lee et al.(2014; 2015a; 2015b; 2015c; 2015d) 및 Yamamoto et al.(1978)과 같은 다수의 연구자들에 의해 유도되었으며, 그들은 진동간극수압과 잔류간극수압을 별개로 취급하여 각 파동에 의한 지반응답을 논의하였다. 그러나, 실제 현장이나 실험에서 해저지반내 간극수압은 진동성분과 잔류성분이 별개로 나타나는 것이 아니고 그의 합 (전간극수압)으로 주어지기 때문에 전간극수압의 관점에서 반드시 검토될 필요가 있다. 따라서, 본 연구에서는 진동간극수압과 잔류간극수압뿐만 아니라 전간극수압의 측면에서 파동조건, 지반조건 및 흐름조건의 변화에 따른 지반응답의 변동특성을 논의하였으며, 더불어 이에 따른 액상화의 연직깊이에서 특성변화를 검토하였다. 이로부터 진행파와 순방향의 흐름의 공존장에서는 흐름속도가 증가할수록 무차원진동간극수압이 증가하고, 무차원잔류간극수압은 감소하여 결과적으로 무차원전간극수압이 작아지며, 무차원액상화 깊이도 감소하는 등의 지반응답특성을 확인할 수 있었다.

• The Plant Pathology Journal
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• 제32권2호
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• pp.112-122
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• 2016

Virus-induced gene silencing (VIGS) is an effective tool for the study of soybean gene function. Successful VIGS depends on the interaction between virus spread and plant growth, which can be influenced by environmental conditions. Recently, we developed a new VIGS system derived from the Soybean yellow common mosaic virus (SYCMV). Here, we investigated several environmental and developmental factors to improve the efficiency of a SYCMV-based VIGS system to optimize the functional analysis of the soybean. Following SYCMV: Glycine max-phytoene desaturase (GmPDS) infiltration, we investigated the effect of photoperiod, inoculation time, concentration of Agrobacterium inoculm, and growth temperature on VIGS efficiency. In addition, the relative expression of GmPDS between non-silenced and silenced plants was measured by qRT-PCR. We found that gene silencing efficiency was highest at a photoperiod of 16/8 h (light/dark) at a growth temperature of approximately $27^{\circ}C$ following syringe infiltration to unrolled unifoliolate leaves in cotyledon stage with a final SYCMV:GmPDS optimal density $(OD)_{600}$ of 2.0. Using this optimized protocol, we achieved high efficiency of GmPDS-silencing in various soybean germplasms including cultivated and wild soybeans. We also confirmed that VIGS occurred in the entire plant, including the root, stem, leaves, and flowers, and could transmit GmPDS to other soybean germplasms via mechanical inoculation. This optimized protocol using a SYCMV-based VIGS system in the soybean should provide a fast and effective method to elucidate gene functions and for use in large-scale screening experiments.

• 자원환경지질
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• 제18권4호
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• pp.369-379
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• 1985

The geological structures and some of the stratigraphy in the area studied are being thought to be ambiguous and controvertible. The present study intended to clarify these ambiguities by correct interpretation of the geological structures and lithostratigraphy of the area concerned. The so-called "Sambangsan formation", which was designated as an unknown age by the Taebaksan Geological Investigation Corps (1962) and as the mid Cambrian age by T. Kobayashi (1966) and I.S. Kim (1983), has been determined by the present study as the $Hongj{\breve{o}}m$ series of Carbo-Permian age resting unconformably on the Cambro-Ordovician limestone formations. This determination was supported by conodont study concurrently carried out by I.S.Kim. The so-called "Daehari formation", which was renamed by the later study group after the original "Sambangsan formation" distributed in the area from southwest of Sambangsan toward southwest to $Juch{\breve{o}}n$, possesses more or less the same lithlogy as "Sambangsan formation" of the old designation in the eastern of the area, but different lithology in the western localities where Sadong formation, the basal sandstone member of the Kobangsan formation and the green shale member of the Nokam formation are cropped out. The narrow belt of the complex mixture of the $Py{\breve{o}}ngan$ group in-between limestone formations extending over 16km with a width of 500m to 1000m was formed by the faults: the northern boundary with the limestone formations is a fault contact all the way through entire area and the southern boundary is either fault contact in most of the area and unconformity in some other area. The $Hongj{\breve{o}}m$ formation on the Mt. Sambangsan shows rather steeply dipping nearly isoclinal folds which plunges $10^{\circ}$ to $20^{\circ}$ southward. There are also field evidences that the limestone formations distributed in both north and south of the Hongjom formation (erstwhile "Sambangsan formation") along the Sambangsan ridge are the same formations and show the same folding as the $Hongj{\breve{o}}m$ formation. Therefore, these limestone formations should be rezoned in the light of the new structural interpretation although they were differently designated in the previous studies as $Py{\breve{o}}ngchang$ and $Y{\breve{o}}ngw{\breve{o}}l$-type of the Joson Group. The structures developed in the area mostly faults, which acted as one of the guides for the new interpretation of the geology and structure of the area are described and shown on the geologic map.

• 자원환경지질
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• 제19권3호
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• pp.225-230
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• 1986

Various interpretations on the boundary between the $Okch{\check{o}}n$ system and the Great Limestone series of the $Chos{\check{o}}n$ system, and on the geologic structure and stratigraphy of the $Okch{\check{o}}n$ system have been yielded by the previous studies, and they are still in hot debate. The present work has mainly studied on the boundary between the $Okch{\check{o}}n$ and $Chos{\check{o}}n$ systems in the south of $Jech{\check{o}}n$, and the geology in its vicinity to clarify the previous misinterpretations if any on the geologic structure and in trun stratigraphy of the area concerned. The boundary between the $Okch{\check{o}}n$ system and the Great Limestone series of the $Chos{\check{o}}n$ system has been thought to be (1) gradational relation which means two systems are the same formation, (2) unconformable relation in which the $Okch{\check{o}}n$ system overlies the $Chos{\check{o}}n$ system, (3) unconformable relation in which the $Chos{\check{o}}n$ system overlies the Okchon system indicating that the age of the $Okch{\check{o}}n$ system is Precambrian, and (4) fault contact in which the $Okch{\check{o}}n$ system of Precambrian age comes in contact with the $Chos{\check{o}}n$ system of Cambro-Ordovician age. The present study clearly found that the relationship between the two systems is a fault zone contact. Shear zone of a width of 300 to 400m is developed, and andesitic volcanics and basic dikes are intruded along the fault zone. This fault contact is exactly the north extension of the Bonghwajae fault, which was denominated long time ago by two of the present authors. The eastern side of the fault has been uplifted so that the $S{\check{o}}changri$ formation of the $Okch{\check{o}}n$ system cropped out in the zone of the Great Limestone series. All the previous workers thought that the $S{\check{o}}changri$ formation rests on the Great Limestone series, but the present study found an overthrust having a strike of $N8^{\circ}E$ and dip of $30^{\circ}NW$ between them, and the $S{\check{o}}changri$ formation has thrusted over the Great Limestone series at the central part of the study area. In the southern and northern parts of this uplifted $S{\check{o}}changri$ formation, the Great Limestone series rests unconformably on it. In the eastern part of the study area where the Mt. Dangdu is located and the previous workers thought that the $S{\check{o}}changri$ formation rests on the Great Limestone series, Precambrian basement rock whose age is older than 1720+50 m.y. crops out in the northern part of the east-west trending high angle fault, and the Great Limestone series rests unconformably on the basement.

• 원예과학기술지
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• 제34권6호
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• pp.860-870
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• 2016

환경요인에 대한 계속적인 모니터링은 농민들에게 온실에서 생육한 작물의 품질과 생산성을 개선할 수 있는 유용한 정보를 제공해 줄 것이다. 이 연구의 목적은 개방형의 저비용 microcontroller를 사용하여 온실 환경 계측 시스템을 개발하기 위함이다. 측정하기 위한 온실 환경 요인들은 대기 온도, 상대습도와 이산화탄소 등이다. 온도, 상대습도와 이산화탄소 농도 측정범위는 $40{\sim}120^{\circ}C$, 0~100%와 0-10,000 ppm이다. 온실 환경 자료를 실시간으로 모니터링하기 위해 $128{\times}64$ 그래픽 LCD을 사용하였다. 컴퓨터와 통신하기 위해 USB 인터페이스를 구성한 아두이노 Uno R3는 6개의 아날로그 입력과 14개의 디지털 입출력 핀으로 구성되어 있다. 온도/습도 센서는 디지털 핀 2번과 3번에 연결하였다. 이산화탄소 센서는 디지털 핀 12번과 13번에 연결하였다. LCD는 디지털 1번(TX)에 연결하였다. 스케치는 아두이노 프로그램 (IDE)로 프로그래밍하였다. 아두이노보드, 센서 및 액세서리 등을 포함한 측정 시스템은 저비용(총 244$)으로 개발되었다. 벤로형 온실에서 환경 요인들은 문제 없이 잘 측정되었다. 우리는 개방형 소프트웨어를 사용한 저비용 microcontroller가 우리 나라의 대부분의 면적을 차지하는 비닐 온실의 대기 환경을 측정하기 위해서 유용하게 사용되리라 예상할 수 있었다.

• KSBB Journal
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• 제24권3호
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• pp.227-238
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• 2009

현재까지의 농약 검출용 바이오센서는 화학 센서, immunoassay, 화학 테스트 킷과 같은 다른 잘 알려진 분석 방법들과 경쟁적으로 연구되어 지고 있다. 바이오센서가 농약을 증명하는 간단하고 저렴한 방법으로 chromatography 방법들을 대체할 잠재력을 가지고 있음에도 불구하고 정확한 정량적 분석 방법이 아직도 미비하다. 안정하고 강력한 바이오센서의 발전을 위해서 유전자 조작을 이용한 효소 개량을 통해 좀 더 민감하고 반응속도가 빠른 생촉매와 특이성이 높은 항체의 개발이 요구되고 있다. 바이오센서의 안정성 개선과 transducer 표면으로부터의 빠른 신호 전달을 위해 새로운 고정화 방법이 탐구되어져야 한다. 비록 약간의 방법들이 시료의 전처리를 필요로 하지 않을 지라도 센서의 안정성은 또 다른 개념으로 접근해야 한다. 그래서 현장 적용을 위해, 보다 간편한 시료의 전처리과정 혹은 직접적인 분석 방식이 동일시되어 개선되어야 한다. 향상된 fabrication 기술을 이용한 소형화 센서 혹은 일회용 킷의 개발은 개인용 및 산업용, 의약용 등의 여러 분야에서 실시간으로 분석이 가능하게 할 것이다. 실제 샘플의 빠르고 자동화 및 소형화된 분석 시스템의 구축을 위해 장차 매우 선택적인 다중 검출 바이오센서의 설계에 더 많은 중점을 둘 필요가 있다. 앞으로 잔류농약 검출을 위한 휴대형 바이오센서의 개발과 상용화를 위해서, 무엇보다 현재의 GC, LC (혹은 GC/MS, LC/MS) 분석을 위해 이루어지고 있는 샘플 전처리 방법의 경우, 다량의 샘플로부터 유기용매 등을 이용한 추출방법으로 진행되고 있기 때문에 샘플 전처리를 간소화하고 간단한 측정방법으로 전체의 측정결과를 대변할 수 있는 방안을 구축하고 잔류농약의 법적 허용기준과 적용이 가능한 방법을 찾아내는 노력이 절실히 요구되는 상황이다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권7호
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• pp.1054-1060
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• 2017

Objective: This study was aimed at evaluating effects of cattle breed resources and alternative mixed-feeding practices on meat productivity and emission intensities from household farming systems (HFS) in Daklak Province, Vietnam. Methods: Records from Local $Yellow{\time}Red$ Sindhi (Bos indicus; Lai Sind) and 1/2 Limousin, 1/2 Drought Master, and 1/2 Red Angus cattle during the growth (0 to 21 months) and fattening (22 to 25 months) periods were used to better understand variations on meat productivity and enteric methane emissions. Parameters were determined by the ruminant model. Four scenarios were developed: (HFS1) grazing from birth to slaughter on native grasses for approximately 10 h plus 1.5 kg dry matter/d (0.8% live weight [LW]) of a mixture of guinea grass (19%), cassava (43%) powder, cotton (23%) seed, and rice (15%) straw; (HFS2) growth period fed with elephant grass (1% of LW) plus supplementation (1.5% of LW) of rice bran (36%), maize (33%), and cassava (31%) meals; and HFS3 and HFS4 computed elephant grass, but concentrate supplementation reaching 2% and 1% of LW, respectively. Results: Results show that compared to HFS1, emissions ($72.3{\pm}0.96kg\;CH_4/animal/life$; least squares $means{\pm}standard$ error of the mean) were 15%, 6%, and 23% lower (p<0.01) for the HFS2, HFS3, and HFS4, respectively. The predicted methane efficiencies ($CO_2eq$) per kg of LW at slaughter ($4.3{\pm}0.15$), carcass weight ($8.8{\pm}0.25kg$) and kg of edible protein ($44.1{\pm}1.29$) were also lower (p<0.05) in the HFS4. In particular, irrespective of the HSF, feed supply and ratio changes had a more positive impact on emission intensities when crossbred 1/2 Red Angus cattle were fed than in their crossbred counterparts. Conclusion: Modest improvements on feeding practices and integrated modelling frameworks may offer potential trade-offs to respond to climate change in Vietnam.