Browse > Article
http://dx.doi.org/10.7846/JKOSMEE.2016.19.4.341

Real-time Micro-algae Flocculation Analysis Method Based on Lens-free Shadow Imaging Technique (LSIT)  

Seo, Dongmin (Department of Electronics and Information Engineering, Korea University)
Oh, Sangwoo (Department of Electronics and Information Engineering, Korea University)
Dong, Dandan (Department of Environmental Engineering, Korea University)
Lee, Jae Woo (Department of Environmental Engineering, Korea University)
Seo, Sungkyu (Department of Electronics and Information Engineering, Korea University)
Publication Information
Journal of the Korean Society for Marine Environment & Energy / v.19, no.4, 2016 , pp. 341-348 More about this Journal
Abstract
Micro-algae, one of the biological resources for alternative energy, has been heavily studied. Among various methods to analyze the status of the micro-algae including counting, screening, and flocculation, the flocculation approach has been widely accepted in many critical applications such as red tide removal study or microalgae resource study. To characterize the flocculation status of the micro-alga. A traditional optical modality, i.e., photospectrometry, measuring the optical density of the flocs has been frequently employed. While this traditional optical method needs shorter time than the counting method in flocculation status analysis, it has relatively lower detection accuracy. To address this issue, a novel real-time micro-algae flocculation analysis method based on the lens-free shadow imaging technique (LSIT) is introduced. Both single cell detection and floc detection are simultaneously available with a proposed lens-free shadow image, confirmed by comparing the results with optical microscope images. And three shadow parameters, e.g., number of flocs, effective area of flocs, and maximum size of floc, enabling quantification of the flocculation phenomenon of micro-alga, are firstly demonstrated in this article. The efficacy of each shadow parameter is verified with the real-time flocculation monitoring experiments using custom developed cohesive agents.
Keywords
Lensfree shadow imaging technique; Micro-algae; Flocculation; Jar test; Floc analyzer;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Aruoja, V., Dubourguier, H.-C., Kasemets, K. and Kahru, A., 2009, "Toxicity of nanoparticles of CuO, ZnO and $TiO_2$ to microalgae Pseudokirchneriella subcapitata", Sci. Total Environ., Vol. 407, No. 4, 1461-1468.   DOI
2 Das, P., Thaher, M. I., Hakim, M. A. Q. M. A., Al-Jabri, H. M. S.J. and Alghasal, G. S. H.S., 2016, "Microalgae harvesting by pH adjusted coagulation-flocculation, recycling of the coagulant and the growth media", Bioresour. Technol., Vol. 216, 824-829.   DOI
3 Franklin, N. M., Stauber, J. L. and Lim, R. P., 2001, "Development of flow cytometry-based algal bioassays for assessing toxicity of copper in natural waters", Environ. Toxicol. Chem., Vol. 20, No. 1, 160-170.   DOI
4 Holm, E. R., Stamper, D. M., Brizzolara, R. A., Barnes, L., Deamer, N. and Burkholder, J. M., 2008, "Sonication of bacteria, phytoplankton and zooplankton: application to treatment of ballast water", Mar. Pollut. Bull., Vol. 56, No. 6, 1201-1208.   DOI
5 Jakob, G., Stephens, E., Feller, R., Oey, M., Hankamer, B. and Ross, I. L., 2016, "Triggered exocytosis of the protozoan tetrahymena as a source of bioflocculation and a controllable dewatering method for efficient harvest of microalgal cultures", Algal Res., Vol. 13, 148-158.   DOI
6 Jin, G., Yoo, I.-H., Pack, S. P., Yang, J.-W., Ha, U.-H., Paek, S.-H. and Seo, S., 2012, "Lens-free shadow image based high-throughput continuous cell monitoring technique", Biosens. Bioelectron., Vol. 38, No. 1, 126-131.   DOI
7 Johnk, K. D., Huisman, J., Sharples, J., Sommeijer, B., Visser, P. M. and Stroom, J. M., 2008, "Summer heatwaves promote blooms of harmful cyanobacteria", Glob. Change Biol., Vol. 14, No. 3, 495-512.   DOI
8 Kim, D.-S., Choi, J.-H., Nam, M.-H., Yang, J.-W., Pak, J. J. and Seo, S., 2011, "LED and CMOS image sensor based hemoglobin concentration measurement technique", Sens. Actuators, B, Vol. 157, 103-109.   DOI
9 Kwak, Y. H., Lee, J., Lee, J., Kwak, S. H., Oh, S., Paek, S.-H., Ha, U.-H. and Seo, S., 2014, "A simple and low-cost biofilm quantification method using LED and CMOS image sensor", J. Microbiol. Methods, Vol. 107, 150-156.   DOI
10 Lee, E. J., Ahn, K.-Y., Lee, J.-H., Park, J.-S., Song, J.-A., Sim, S. J., Lee, E. B., Cha, Y. J. and Lee, J., 2012, "A novel bioassay platform using ferritin-based nanoprobe hydrogel", Adv. Mater., Vol. 24, No. 35, 4739-4744.   DOI
11 Lee, J., Kwak, Y. H., Paek, S.-H., Han, S. and Seo, S., 2014, "CMOS image sensor-based ELISA detector using lens-free shadow imaging platform", Sens. Actuators, B, Vol. 196, 511-517.   DOI
12 Moreno-Garrido, I., Lubian, L. M. and Soares, A. M. V. M., 2000, "Influence of cellular density on determination of EC50 in microalgal growth inhibition tests", Ecotoxicol. Environ. Saf., Vol. 47, No. 2, 112-116.   DOI
13 Lee, Y.-C., Jin, E.S., Jung, S. W., Kim, Y.-M., Chang, K. S., Yang, J.-W., Kim, S.-W., Kim, Y.-O. and Shin, H.-J., 2013, "Utilizing the algicidal activity of aminoclay as a practical treatment for toxic red tides", Sci. Rep., Vol. 3, No. 1292, 1-8.
14 Li, M., Zhu, W. and Gao, L., 2014, "Analysis of cell concentration, volume concentration, and colony size of microcystis via laser particle analyzer", Environ. Manage., Vol. 53, No. 5, 947-958.   DOI
15 Mata, T. M., Martins, A. A. and Caetano, N. S., 2010, "Microalgae for biodiesel production and other applications: a review", Renewable Sustainable Energy Rev., Vol. 14, No. 1, 217-232.   DOI
16 Ozcan, A. and Demirci, U., 2007, "Ultra wide-field lens-free monitoring of cells on-chip", Lab Chip, Vol. 8, No. 1, 98-106.   DOI
17 Patil, P. D., Gude, V. G., Mannarswamy, A., Deng, S., Cooke, P., Munson-McGee, S., Rhodes, I., Lammers, P. and Nirmalakhandan, N., 2011, "Optimization of direct conversion of wet algae to biodiesel under supercritical methanol conditions", Bioresour. Technol., Vol. 102, No. 1, 118-122.   DOI
18 Promdaen, S., Wattuya, P. and Sanevas, N., 2014, "Automated microalgae image classification", Procedia Comput. Sci., Vol. 29, 1981-1992.   DOI
19 Roy, M., Seo, D., Oh, C.-H., Nam, M.-H., Kim, Y. J. and Seo, S., 2015, "Low-cost telemedicine device performing cell and particle size measurement based on lens-free shadow imaging technology", Biosens. Bioelectron., Vol. 67, 715-723.   DOI
20 Reddy, K. R., 1981, "Diel variations of certain physico-chemical parameters of water in selected aquatic systems", Hydrobiologia, Vol. 85, No. 3, 201-207.   DOI
21 Sengco, M. R. and Anderson, D. M., 2004, "Controlling harmful algal blooms through clay flocculation", J. Eukaryor. Microbiol., Vol. 51, No. 2, 169-172.   DOI
22 Seo, S., Su, T.-W., Tseng, D. K., Erlinger, A. and Ozcan, A., 2008, "Lensfree holographic imaging for on-chip cytometry and diagnostics", Lab Chip, Vol. 9, No. 6, 777-787.   DOI
23 Shin, Y.-H, Barnett, J. Z., Song, E., Gutierrez-Wing, M. T., Rusch, K. A. and Choi, J.-W., 2015, "A portable fluorescent sensor for on-site detection of microalgae", Microelectron. Eng., Vol. 144, 6-11.   DOI
24 Spolaore, P., Joannis-Cassan, C., Duran, E. and Isambert, A., 2006, "Commercial applications of microalgae", J. Biosci. Bioeng., Vol. 101, No. 2, 87-96.   DOI
25 Stumplf, R. P., Tomlinson, M. C., Calkins, J. A., Kirkpatrick, B., Fisher, K., Nierenberg, K., Currier, R. and Wynne, T. T., 2009, "Skill assessment for an operational algal bloom forecast system", J. Marine Syst., Vol. 76, No. 1-2, 151-161.   DOI
26 Stybayeva, G., Mudanyali, O., Seo, S., Silangcruz, J., Macal, M., Ramanculov, E., Dandekar, S., Erlinger, A., Ozcan, A. and Revzin, A., 2010, "Lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function", Anal. Chem., Vol. 82, No. 9, 3736-3744.   DOI
27 Tassinari, B., Conaghan, S., Freeland, B. and Marison, I. W., 2015, "Application of turbidity meters for the quantitative analysis of flocculation in a jar test apparatus", J. Environ. Eng., Vol. 149, No. 9, 04015015-1-04015015-8.
28 Wang, B., Li, Y., Wu, N. and Lan, C. Q., 2008, "CO2 bio-mitigation using microalgae", Appl. Microbiol. Biotechnol., Vol. 79, No. 5, 707-718.   DOI
29 Usov, A. I., Smirnova, G. P. and Klochkova, N. G., 2001, "Polysaccharides of Algae: 55. polysaccharide composition of several brown algae from kamchatka", Russ. J. Bioorg. Chem., Vol. 27, No. 6, 444-448.
30 Vandamme, D., Pontes, S. C. V., Goiris, K., Foubert, I., Pinoy, L. J. J. and Muylaert, K., 2011, "Evaluation of electro-coagulationflocculation for harvesting marine and freshwater microalgae", Biotechnol. Bioeng., Vol. 108, No. 10, 2320-2329.   DOI
31 Williams, P. J. I. B. and Laurens, L. M. L., 2010, "Microalgae as biodiesel & biomass feedstocks: review & analysis of the biochemistry, energetics & economics", Energy Environ. Sci., Vol. 3, No. 5, 554-590.   DOI
32 Woelkerling, W. J., Kowal, R. R. and Gough, S. B., 1976, "Sedgwick-rafter cell counts: a procedural analysis", Hydrobiologia, Vol. 48, No. 2, 95-107.   DOI