• 한국식품과학회지
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• 제36권2호
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• pp.189-195
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• 2004

본 연구에서는 조제분유 중에 영양강화를 위해 첨가하는 비타민 A와 E의 함량측정을 위해 각기 다른 실험방법을 사용하지 않고 2가지 비타민을 동시에 분석하는 신속분석법을 위와 같이 수행하였다. 비타민 A와 E를 유기용매로 동시에 신속하게 추출하고 역상컬럼과 PDA-HPLC를 이용하여 각각의 성분으로 모두 분리한 후 동시에 검출하는 방법을 사용하였다. 국제표준인증물질 및 조제분유를 시료로 사용하여 본 연구의 실험방법에 의해 측정된 값을 식품공전방법 및 AOAC방법에 의한 측정값과 비교한 결과 유사한 측정결과를 얻을 수 있었다. 또한 본 연구의 실험방법에 의하여 수행한 국제인증표준물질중의 비타민 A와 E의 함량측정 결과는 인증된 표준값내의 결과를 보여주었다. 따라서, 비타민 A또는 E를 강화한 분유, 이유식 등의 분말 유제품 중에서 비타민 A와 E의 함량을 측정 하고자 할 때 한정된 장비와 인력으로 각각의 2가지 실험방법을 수행하기가 어렵거나 시간단축이 필요한 경우, 본 연구에서 수행한 실험방법과 같이 시료 전처리를 간단하고 신속하게 수행할 뿐만 아니라 역상컬럼과 PDA-HPLC에 의해 비타민 A와 E를 동시에 분석함으로써 보다 효율적인 분석을 진행 할 수 있을 것으로 사료된다.

• Natural Product Sciences
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• 제24권3호
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• pp.206-212
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• 2018

Xanthii Fructus has been traditionally used for the treatment of rhinitis, rheumatoid arthritis, and eczema. In this study, a high-performance liquid chromatography-photodiode array (HPLC-PDA) method was developed and then used for the simultaneous analysis of eight phenylpropanoids in Xanthii Fructus. The analytical column used for this separation was a $SunFire^{TM}$ $C_{18}$ column, maintained at $40^{\circ}C$. The mobile phase used was 1.0% acetic acid in distilled water and 1.0% acetic acid in acetonitrile with gradient elution. For identify of each component, the mass spectrometer (MS) was used a Waters triple quadrupole mass spectrometer requipped with electrospray ionization (ESI) source. The HPLC-PDA method showed good linearity: correlation coefficients were ${\geq}0.9996$. The limits of detection and quantification of the eight compounds were 0.02 - 0.04 and $0.06-0.14{\mu}g/mL$, respectively. The extraction recoveries ranged from 97.51 to 108.67%. The relative standard deviation values of intra- and inter-day precision were 0.06 - 1.55 and 0.09 - 1.68%, respectively. The validated HPLC-PDA method was applied to simultaneously analyse the amounts of eight phenlypropanoids in Xanthii Fructus.

• Natural Product Sciences
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• 제25권3호
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• pp.275-283
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• 2019

In this study, we described the new developed method to simultaneously discriminate two herbal drugs of Artemisiae Argyi Folium and Artemisiae Iwayomogii Herba using eight marker compounds (1 - 8) on an HPLC-PDA system. The developed method was applied to quantify the major components of two herbal drugs. The pattern analysis successfully discriminated and evaluated different components between Artemisiae Argyi Folium and Artemisiae Iwayomogii Herba. Results were used for classification of different species from collected samples.

• 생약학회지
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• 제42권3호
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• pp.240-245
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• 2011

A high-performance liquid chromatography (HPLC) method was developed for quantitative analysis of liquiritin and glycyrrhizin in Sagunja-tang (SGT, Sijunzi-tang in Chinese), a traditional Korean medicine. HPLC analysis was performed using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array (PDA) detection at 254 nm and 280 nm for quantification of the two components in SGT. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Calibration curves were acquired with $r^2$ values > 0.9998, and the relative standard deviations (RSDs, %) for intra- and inter-day precision were not exceed 4.0%. The recovery of each component was in the range of 91.85 - 108.62%, with a RSD less than 4.0%. The contents of the two components in SGT were 7.94 - 13.83 mg/g.

• Natural Product Sciences
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• 제25권2호
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• pp.122-129
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• 2019

The roots of Phlomis umbrosa (Turcz.) (Phlomidis Radix) have been traditionally used to treat cold, reduce swelling and staunch bleeding. Four iridoids (1 - 3 and 5) and six phenylethanoid derivatives (4, and 6 - 10) were isolated from the roots of P. umbrosa. A simple, sensitive, and reliable analytical HPLC/PDA method was developed, validated, and applied to determine 10 marker compounds in Phlomidis Radix. Furthermore, the isolates were evaluated for cytotoxic and anti-oxidant activities as well as DPPH-HPLC method. Among them, compounds 4 and 6 - 9 displayed potent anti-oxidant capacities using DPPH assay with $IC_{50}$ values of $27.7{\pm}2.4$, $10.2{\pm}1.1$, $18.0{\pm}0.8$, $19.1{\pm}0.3$, and $19.9{\pm}0.6{\mu}M$, and compounds 6, 8, and 9 displayed significant cytotoxic activity against HL-60 with $IC_{50}$ values of $35.4{\pm}3.1$, $18.6{\pm}2.0$, and $42.9{\pm}3.0{\mu}M$, respectively.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.67-72
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• 2023

A simple and accurate method was developed for the quantitative analysis of oleanonic acid (OA) from mastic gum. The analysis was carried out using reverse-phase high-performance liquid chromatography combined with a photodiode array detector (HPLC/PDA). Our optimized method was validated by measuring various parameters, using an INNO C18 column fitted with a gradient elution system. The results revealed limits of detection and quantification of 0.34 and 1.042 ㎍/mL, respectively. The OA calibration curve exhibited excellent linearity over the concentration range of 0.0625 to 2.0 mg/mL, with r² =0.9996. Accuracy tests revealed a high recovery rate of 99.44-103.66%, with precision values below 0.15%. These results suggest that the present analytical method can identify and quantify OA in mastic gum with high precision. The HPLC approach developed in this study might be applied to routine analyses and large-scale extraction procedures for OA content quantification.

• Natural Product Sciences
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• 제25권4호
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• pp.334-340
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• 2019

Cuscuta chinensis Lam. and Cuscuta japonica Choisy are parasitic plants. C. chinensis seeds were traditionally used for treatment of kidney and liver deficiencies. C. japonica seeds were used as tonic medicine to improve liver function and strengthen kidneys, treatment of high blood pressure, chronic diarrhea, and sore eyes. Cuscutae Semen are seeds of only C. chinensis in Korean Herbal Pharmacopoeia (K.H.P.). The developed HPLC-PDA method easily, accurately, and sensitively quantified using eight marker compounds [hyperoside (1), astragalin, (2), quercetin (3), kaempferol (4), chlorogenic acid (5), 3,4-di-O-caffeoylquinic acid (6), 1,5-di-O-caffeoylquinic acid (7), and 4,5-di-O-caffeoylquinic acid (8)]. In addition, the method may be used to distinguish seeds between C. chinensis Lam. and C. japonica Choisy. Furthermore, the result from the current study was applied to clarify samples between steam processed and unprocessed samples of C. chinensis by pattern analysis.

• 한국동물위생학회지
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• 제29권3호
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• pp.339-346
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• 2006

Semi-micro-HPLC using a column-switching technique was developed for simultaneous determination of vitamin A and E contents in infant formula. Vitamin A and E were extracted by PDA - HPLC with reversed phase column using organic solvent and their contents in Certified Reference Material (CRM) and infant formula were determined and compared with hydrolysis method and rapid extraction. Developed method has many advantages of simple and rapid sample preparation and simultaneous determination of vitamin A and E by micro-HPLC using reversed phase column.

• 한국식품영양과학회지
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• 제33권3호
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• pp.549-552
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• 2004

본 연구는 식품 중 식품에 사용이 불가한 수단 1호, 2호, 3호 및 4호 등 4종의 유용성 색소에 대한 안전성 확보 및 효율적 품질관리를 위해 박층크로마토그래피 (TLC)와 고속액체 크로마토그래피 (HPLC-PDA)를 이용한 정성, 정량분석법을 확립하고자 수행하였다. $C_{18}$-silica를 사용하여 methanol : water(95 : 5)으로 전개하였을 때 수단색소들의 R$_{f}$ 값은 0.27～0.59로 산출되었다. HPLC-PDA를 이용하여 water와 acetonitrile용매를 사용하여 분석한 결과 20분내에 모두 분리되었으며 검량선의 농도 범위는 0.1 ∼ 100.0 $\mu\textrm{g}$/mL으로 나타났다. 본 연구에서 고춧가루, 김치 및 깍두기 등 식품을 대상으로 적용한 결과, 회수율은 고춧가루에서는 85.84∼96.95%, 김치에서는 86.24∼104.30%, 깍두기에서는 83.02∼99.93%의 양호한 결과를 얻었으며 , 검출한계는 수단 1호 및 2호는 0.01 $\mu\textrm{g}$/mL, 수단3호 및 4호는 0.02 $\mu\textrm{g}$/mL으로 나타났다.

• 대한수의학회지
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• 제48권1호
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• pp.9-16
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• 2008

A method for the quantification of eugenol in the medicinal herb Clove was developed and validated. For preparation of sample solutions clove was dried at $60^{\circ}C$ for 2h and ground by mixer and extracted with 95% ethanol for shaking extraction. The elutes were analyzed by HPLC system included a reversed phase column, a isocratic mobile phase of 60% methanol and PDA detector set at 280 nm. Calibration graphs were linear with very good correlation coefficients ($r^2>0.9999$) from $0.0125~1{\mu}g/ml$. The limit of detection per sample injection ($20{\mu}l$) was $0.81ng/{\mu}l$ and limit of quantification was $2.47ng/{\mu}l$. The method showed good intra-day precision (%RSD 0.08 ~ 0.27%) and inter-day precision (%RSD 0.32 ~ 1.19%).