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Simultaneous Analysis of Liquiritin and Glycyrrhizin in Sagunja-tang by HPLC-PDA  

Seo, Chang-Seob (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Kim, Jung-Hoon (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Pharmacognosy / v.42, no.3, 2011 , pp. 240-245 More about this Journal
Abstract
A high-performance liquid chromatography (HPLC) method was developed for quantitative analysis of liquiritin and glycyrrhizin in Sagunja-tang (SGT, Sijunzi-tang in Chinese), a traditional Korean medicine. HPLC analysis was performed using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array (PDA) detection at 254 nm and 280 nm for quantification of the two components in SGT. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Calibration curves were acquired with $r^2$ values > 0.9998, and the relative standard deviations (RSDs, %) for intra- and inter-day precision were not exceed 4.0%. The recovery of each component was in the range of 91.85 - 108.62%, with a RSD less than 4.0%. The contents of the two components in SGT were 7.94 - 13.83 mg/g.
Keywords
HPLC-PDA; Sagunja-tang (SGT); Simultaneous analysis; Herbal medicine;
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