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Agricultural and Quality Characteristics in Recombinant Inbred Lines (RILs) Population in Perilla (Perilla frutescens) (들깨 대실/잎들깨1호 재조합 자식계통(RILs)의 농업적 특성 및 품질 분석)

  • Park, Jae Eun;Lee, Myoung Hee;Oh, Ki Won;Kim, Sungup;Oh, Eunyoung;Ha, Tae Joung;Cho, Kwang-Soo;Jung, Chan Sik;Kim, Jung In
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.3
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    • pp.248-255
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    • 2021
  • This study was conducted to obtain basal information for the development of perilla cultivars with improved quality. The F7 population of recombinant inbred lines (RILs) from a cross between the parents of Daesil for seeds and Ipdeulkkae1 for vegetables was used as the material. We evaluated several agricultural characteristics and seed quality. Variations were observed in most of the measurements; for example, stem length (ranging from 66.0 to 150.0 cm), number of branches (from 5 to 23), flower cluster length (ranging from 5.1 to 10.5 cm), number of flower clusters (from 17 to 131), a-linolenic acid content (from 54.2 to 64.1%), and functional compound content (rosmarinic acid 869.5~3,508.1 ㎍/g; luteolin 47.4~864.3 ㎍/g; apigenin 57.1~296.7 ㎍/g) all showed variation. Significant correlations between stem length and the number of branches (0.561) and number of branches versus number of flower clusters (0.638) were detected in the RIL F7 population. Most agricultural characteristics and seed qualities showed a normal distribution with large variation, and transgressive segregation was observed in many descendants with characteristics to those of their parents. Daesil/Ipdeulkkae1 RIL F7 populations could be useful for future QTL analysis as well as for intermediate breeding lines for high-quality perilla cultivars.

Monitoring for Microbiological Quality of Rice Cakes Manufactured by Small-Scale Business in Korea (소규모 가공경영체 떡류의 생산과정에 따른 미생물학적 품질조사를 위한 모니터링)

  • Han, Sangha;Kim, Kyeongjun;Byun, Kye-Hwan;Kim, Duk-Hyun;Choi, Song-yi;Ha, Sang-do
    • Journal of Food Hygiene and Safety
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    • v.36 no.5
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    • pp.400-406
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    • 2021
  • The purpose of this study was to evaluate the microbial contamination level of Korean traditional rice cakes (Garaetteok, Injeolmi, Gyeongdan), as well as manufacturing environment of small-sized businesses in Korea. The contamination levels of total aerobic bacteria, coliforms, and Bacillus cereus in raw materials were 3.76-4.48, 2.21-4.14, and 1.02-1.15 log CFU/g respectively. On the other hand, Escherichia coli was not found. It has been found that the contamination level of total aerobic bacteria, coliforms, and B. cereus in the raw material decreased after the washing process, but it increased again during the soaking and grinding process. However, after the steaming stage, the contamination level increased again during the molding and cooling process, suggesting the need to take cautions in managing cooling water and molded rice cakes in the process. These results suggest that the safe management of cooling water and taking cautions in the drying process after steaming of rice cakes are necessary for controlling cross-contamination. No E. coli was detected during the manufacturing process involving all tested rice cakes. The microbial contamination level of manufacturing environment such as rice grinder and rice cake forming machine was high. Therefore, in terms of food safety strategy, it is necessary to consider introducing systematic cleansing and disinfection procedure to processing equipment and environment for the sake of reducing microbiological risks.

Comparison of ginsenoside contents and antioxidant activity according to the size of ginseng sprout has produced in a plant factory (식물공장에서 생산된 새싹인삼의 크기에 따른 진세노사이드 함량 및 항산화 활성 비교)

  • Hwang, Seung Ha;Kim, Su Cheol;Seong, Jin A;Lee, Hee Yul;Cho, Du Yong;Kim, Min Ju;Jung, Jea Gack;Jeong, Eun Hye;Son, Ki-Ho;Cho, Kye Man
    • Journal of Applied Biological Chemistry
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    • v.64 no.3
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    • pp.253-261
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    • 2021
  • In this study, the ginseng sprout has produced through smart farm was classified according to its size and divided into above-ground (AG) and below-ground (BG) parts to compare ginsenoside contents and antioxidant activity. In the case of the AG part, the total phenolic contents were the highest at 5.16 mg/g in medium (M) size and the lowest at 2.23 mg/g in largest (L) size. The BG part also showed the highest content in the M size, but there was no significant difference. Also, the total flavonoid contents were also high in the M size in both the AG (5.16 mg/g) and BG (1.28 mg/g) parts. The major ginsenosides in the AG part were Re (20.33-24.15 mg/g) > Rd (11.36-27.42 mg/g) > Rg1 (4.48-5.54 mg/g) and the main ginsenosides in the BG part were Rb1 (5.09-8.61 mg/g) > Re (4.48-5.54 mg/g) > Rc (3.11-4.11 mg/g) in orders. In the case of M size, Re and Rd were approximately 4- and 19-folds higher at 24.15 mg/g and at 27.42 mg/g in the AG part and 5.20 mg/g and 1.43 mg in the BG part, respectively. In addition, F3 and Rh1 were detected in the AG part, but not in the BG part. 2,2-diphenyl-1-picrylhydrazyl (74.95%), 2,4,6-azino-bis (3-ethylbenzothiazoline-6-sulphnoic acid) diammonium salt (94.47%), and hydroxyl (70.39%) radical scavenging activities and FRAP (2.169) assay were the highest in M size than other sizes.

Analysis of Building Characteristics and Temporal Changes of Fire Alarms (건물 특성과 시간적 변화가 소방시설관리시스템의 화재알람에 미치는 영향 분석 연구)

  • Lim, Gwanmuk;Ko, Seoltae;Kim, Yoosin;Park, Keon Chul
    • Journal of Internet Computing and Services
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    • v.22 no.4
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    • pp.83-98
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    • 2021
  • The purpose of this study to find the factors influencing the fire alarms using IoT firefighting facility management system data of Seoul Fire & Disaster Headquarters, and to present academic implications for establishing an effective prevention system of fire situation. As the number of high and complex buildings increases and former bulidings are advanced, the fire detection facilities that can quickly respond to emergency situations are also increasing. However, if the accuracy of the fire situation is incorrectly detected and the accuracy is lowered, the inconvenience of the residents increases and the reliability decreases. Therefore, it is necessary to improve accuracy of the system through efficient inspection and the internal environment investigation of buildings. The purpose of this study is to find out that false detection may occur due to building characteristics such as usage or time, and to aim of emphasizing the need for efficient system inspection and controlling the internal environment. As a result, it is found that the size(total area) of the building had the greatest effect on the fire alarms, and the fire alarms increased as private buildings, R-type receivers, and a large number of failure or shutoff days. In addition, factors that influencing fire alarms were different depending on the main usage of the building. In terms of time, it was found to follow people's daily patterns during weekdays(9 am to 6 pm), and each peaked around 10 am and 2 pm. This study was claimed that it is necessary to investigate the building environment that caused the fire alarms, along with the system internal inspection. Also, it propose additional recording of building environment data in real-time for follow-up research and system enhancement.

A Study on the Possibility of Short-term Monitoring of Coastal Topography Changes Using GOCI-II (GOCI-II를 활용한 단기 연안지형변화 모니터링 가능성 평가 연구)

  • Lee, Jingyo;Kim, Keunyong;Ryu, Joo-Hyung
    • Korean Journal of Remote Sensing
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    • v.37 no.5_2
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    • pp.1329-1340
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    • 2021
  • The intertidal zone, which is a transitional zone between the ocean and the land, requires continuous monitoring as various changes occur rapidly due to artificial activity and natural disturbance. Monitoring of coastal topography changes using remote sensing method is evaluated to be effective in overcoming the limitations of intertidal zone accessibility and observing long-term topographic changes in intertidal zone. Most of the existing coastal topographic monitoring studies using remote sensing were conducted through high spatial resolution images such as Landsat and Sentinel. This study extracted the waterline using the NDWI from the GOCI-II (Geostationary Ocean Color Satellite-II) data, identified the changes in the intertidal area in Gyeonggi Bay according to various tidal heights, and examined the utility of DEM generation and topography altitude change observation over a short period of time. GOCI-II (249 scenes), Sentinel-2A/B (39 scenes), Landsat 8 OLI (7 scenes) images were obtained around Gyeonggi Bay from October 8, 2020 to August 16, 2021. If generating intertidal area DEM, Sentinel and Landsat images required at least 3 months to 1 year of data collection, but the GOCI-II satellite was able to generate intertidal area DEM in Gyeonggi Bay using only one day of data according to tidal heights, and the topography altitude was also observed through exposure frequency. When observing coastal topography changes using the GOCI-II satellite, it would be a good idea to detect topography changes early through a short cycle and to accurately interpolate and utilize insufficient spatial resolutions using multi-remote sensing data of high resolution. Based on the above results, it is expected that it will be possible to quickly provide information necessary for the latest topographic map and coastal management of the Korean Peninsula by expanding the research area and developing technologies that can be automatically analyzed and detected.

Association Analysis of the 6-bp Deletion Variant of the MYH3 Gene with Meat Color Traits in Crossbred (Landrace × Jeju Native Black Pig) Pigs (제주재래흑돼지와 랜드레이스 교배 축군에서 MYH3 유전자의 6-bp 결실 변이와 육색 형질간의 연관성 분석)

  • Kang, Yong-Jun;Kim, Sang-Geum;Kim, Su-Yeon;Kim, Min-Ji;Kim, Hyeon-Ah;Shin, Moon-Cheol;Yoo, Ji-Hyun;Yang, Byoung-Chul;Cho, In-Cheol
    • Journal of Life Science
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    • v.31 no.7
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    • pp.626-630
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    • 2021
  • This study was conducted to examine the association between the myosin heavy chain 3 (MYH3) in 6-bp deletion variant genotypes and meat color traits in a crossbred pig population Landrace and Jeju native black pigs (JNBP). The longissimus dorsi, semimembranosus, triceps brachii and biceps femoris muscle from each carcass were used for the analysis of meat color traits. A total of 187 pigs and three meat color traits, CIE L* (lightness), CIE a* (redness), and CIE b* (yellowness), were analyzed. All experimental pigs were successfully genotyped for the MYH3 6-bp deletion variant using Polymerase chain reaction (PCR) analysis. We detected three MYH3 6-bp deletion variant genotypes qq, Qq, and QQ with 0.091, 0.551 and 0.358 genotype frequencies, respectively. Compared to qq homozygotes, the MYH3 6-bp deletion QQ genotype animals showed a higher levels of the meat colors traits CIE L* (lightness), CIE a* (redness), and CIE b* (yellowness) in longissimus dorsi (p>0.05, p<0.001, p<0.001), semimembranosus (p>0.05, p<0.001, p<0.001), triceps brachii (p<0.001, p<0.001, p<0.001), and biceps femoris (p<0.01, p<0.001, p<0.001), respectively. The QQ genotype pigs was associated with increasing meat color traits in the crossbred between Landrace and JNBP. Our findings suggest that the MYH3 6-bp deletion variant genotypes can be used as valuable genetic markers for JNBP-related breeding programs to improve meat quality and control meat color traits.

Host-Based Intrusion Detection Model Using Few-Shot Learning (Few-Shot Learning을 사용한 호스트 기반 침입 탐지 모델)

  • Park, DaeKyeong;Shin, DongIl;Shin, DongKyoo;Kim, Sangsoo
    • KIPS Transactions on Software and Data Engineering
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    • v.10 no.7
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    • pp.271-278
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    • 2021
  • As the current cyber attacks become more intelligent, the existing Intrusion Detection System is difficult for detecting intelligent attacks that deviate from the existing stored patterns. In an attempt to solve this, a model of a deep learning-based intrusion detection system that analyzes the pattern of intelligent attacks through data learning has emerged. Intrusion detection systems are divided into host-based and network-based depending on the installation location. Unlike network-based intrusion detection systems, host-based intrusion detection systems have the disadvantage of having to observe the inside and outside of the system as a whole. However, it has the advantage of being able to detect intrusions that cannot be detected by a network-based intrusion detection system. Therefore, in this study, we conducted a study on a host-based intrusion detection system. In order to evaluate and improve the performance of the host-based intrusion detection system model, we used the host-based Leipzig Intrusion Detection-Data Set (LID-DS) published in 2018. In the performance evaluation of the model using that data set, in order to confirm the similarity of each data and reconstructed to identify whether it is normal data or abnormal data, 1D vector data is converted to 3D image data. Also, the deep learning model has the drawback of having to re-learn every time a new cyber attack method is seen. In other words, it is not efficient because it takes a long time to learn a large amount of data. To solve this problem, this paper proposes the Siamese Convolutional Neural Network (Siamese-CNN) to use the Few-Shot Learning method that shows excellent performance by learning the little amount of data. Siamese-CNN determines whether the attacks are of the same type by the similarity score of each sample of cyber attacks converted into images. The accuracy was calculated using Few-Shot Learning technique, and the performance of Vanilla Convolutional Neural Network (Vanilla-CNN) and Siamese-CNN was compared to confirm the performance of Siamese-CNN. As a result of measuring Accuracy, Precision, Recall and F1-Score index, it was confirmed that the recall of the Siamese-CNN model proposed in this study was increased by about 6% from the Vanilla-CNN model.

Nutritional Analyses and Antioxidant Activity of Apple Pomace (사과 부산물의 영양성분 분석 및 항산화 효과)

  • Kim, Jieun;Shin, Jiyoung;Yang, Ji-Young
    • Journal of Life Science
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    • v.31 no.7
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    • pp.617-625
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    • 2021
  • To enhance the applications of apple pomace, which is a by-product of apple, this study analyzed the nutritional components, ursolic acid content, and antioxidant activity of different solvent (distilled water, fermented alcohol, and methanol) extracts. The samples included hot air-dried and freeze-dried apple pomace. The moisture, protein, fat, ash, and total dietary fiber contents of hot air-dried apple pomace were 3.2%, 3.9%, 2.4%, 2.0%, and 28.5%, respectively, and those of freeze-dried apple pomace were 8.2%, 3.4%, 2.4%, 1.8%, and 33.0%, respectively. Ursolic acid was not detected in the distilled water extract of either sample. However, in hot air-dried apple pomace, the methanol extract was 1,753.32 ㎍/ml, and the fermented alcohol extract was 1,532.94 ㎍/ml. In freeze-dried apple pomace, the methanol extract was 1,407.04 ㎍/ml, and the fermented alcohol extract was 1,221.81 ㎍/ml. The total polyphenol and flavonoid contents were 306.7 ㎍/ml and 950.1 ㎍/ml, respectively in methanol extracts of hot air-dried apple pomace and 277.6 ㎍/ml and 925.0 ㎍/ml, respectively in methanol extracts of freeze-dried apple pomace. 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities of hot air-dried apple pomace were 73.3% in methanol extract and 59.4% in fermented alcohol extract, and those of freeze-dried apple pomace were 76.1% in methanol extract and 66.0% in fermented alcohol extract. Both samples had the lowest antioxidant activity in distilled water extracts. Similar to DPPH radical scavenging activity, both samples showed increasing 2,2'-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid) (ABTS) radical scavenging activity in the order of methanol, fermented alcohol, and distilled water. All samples had stronger reducing power than ascorbic acid (311.5 ㎍/ ml) as a positive control.

Effect of Gamma-ray on Survival and Mutation Rates of Rooted Cuttings and Unrooted Cuttings in Rose (감마선 처리에 의한 장미 삽수의 발근 여부에 따른 생존율과 돌연변이 발생빈도)

  • Kim, Se Won;Lee, Hyo Jeong;Kim, Ye-Sol;Jo, Yeong Deuk;Ryu, Jai Hyunk;Kang, Si-Yong;Kim, Sang Hoon
    • Korean Journal of Breeding Science
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    • v.49 no.3
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    • pp.150-156
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    • 2017
  • This study was carried out to compare the survival and mutation rates and mutation spectrum by gamma-irradiation on rooted and unrooted cuttings of three spray type ('Lovelydia', 'Yellowbabe', and 'Haetsal') and two standard type ('Vital' and 'Aqua') cultivars in roses. Two groups, rooted and unrooted cuttings were gamma-irradiated at 70Gy for 24 hours. The irradiated rooted and unrooted cuttings were planted in a greenhouse, and survival, mutation rates and mutation spectrum were investigated 30 weeks after planting, respectively. As a result, survival and mutation rates of gamma-ray irradiated plants were 16.4%~50.8% and 0~5.1% for unrooted cuttings, and 39.4%~55.1% and 0.7%~7.4% for rooted cuttings, respectively. In conclusion, both survival and mutation rates were a little higher on rooted cuttings than on unrooted cuttings. However, when only survived plants after gamma-ray irradiation were considered, mutation rates were 0~10% and 1.8%~14.1% for unrooted cuttings and rooted cuttings, respectively, showing no significant difference. In addition, diverse variations on color and number of petals or shape of flowers were detected both in plants from rooted and unrooted cuttings, which indicated that there was no significant difference in mutation spectrum between two groups.

Genetic Variation of Parental Inbred Lines for Korean Waxy Corn Hybrid Varieties revealed by SSR markers (우리나라 찰옥수수 품종들의 교배친 자식계통들에 대한 유전적 변이성)

  • Park, Jun-Sung;Sa, Kyu-Jin;Park, Ki Jin;Jang, Jin-Sun;Lee, Ju Kyong
    • Korean Journal of Breeding Science
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    • v.41 no.2
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    • pp.106-114
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    • 2009
  • In maize, knowledge of genetic diversity and genetic relationships among elite inbred lines is an significant impact on the selection of parental lines for hybrid varieties. Genetic diversity and genetic relationships among 11 parental inbred lines of Korean waxy and normal corn varieties were analyzed using 50 SSR markers distributed over the whole genome. A total of 171 allele bands were detected with an average of 3.4 alleles per locus. Number of allele bands per locus ranged from two to six and gene diversity varied from 0.165 to 0.900 with an average of 0.596 depending on the SSR loci. The cluster tree recognized three major groups with 61.6% genetic similarity. Group I includes 7 inbred lines (KL103, HW1, HW4, HW6, HW7, HW8, HW9), with similarity coefficients of between 0.616 and 0.730. Group II includes 2 inbred lines (HF1, HF2), with similarity coefficients of 0.959. Group III includes 2 inbred lines (HW3, HW5), with similarity coefficients of 0.713. The present study indicates that the SSR markers chosen for this analysis are effective for the assessment of genetic diversity and genetic relationships among 11 parental inbred lines.