Browse > Article
http://dx.doi.org/10.5352/JLS.2021.31.7.626

Association Analysis of the 6-bp Deletion Variant of the MYH3 Gene with Meat Color Traits in Crossbred (Landrace × Jeju Native Black Pig) Pigs  

Kang, Yong-Jun (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
Kim, Sang-Geum (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
Kim, Su-Yeon (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
Kim, Min-Ji (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
Kim, Hyeon-Ah (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
Shin, Moon-Cheol (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
Yoo, Ji-Hyun (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
Yang, Byoung-Chul (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
Cho, In-Cheol (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
Publication Information
Journal of Life Science / v.31, no.7, 2021 , pp. 626-630 More about this Journal
Abstract
This study was conducted to examine the association between the myosin heavy chain 3 (MYH3) in 6-bp deletion variant genotypes and meat color traits in a crossbred pig population Landrace and Jeju native black pigs (JNBP). The longissimus dorsi, semimembranosus, triceps brachii and biceps femoris muscle from each carcass were used for the analysis of meat color traits. A total of 187 pigs and three meat color traits, CIE L* (lightness), CIE a* (redness), and CIE b* (yellowness), were analyzed. All experimental pigs were successfully genotyped for the MYH3 6-bp deletion variant using Polymerase chain reaction (PCR) analysis. We detected three MYH3 6-bp deletion variant genotypes qq, Qq, and QQ with 0.091, 0.551 and 0.358 genotype frequencies, respectively. Compared to qq homozygotes, the MYH3 6-bp deletion QQ genotype animals showed a higher levels of the meat colors traits CIE L* (lightness), CIE a* (redness), and CIE b* (yellowness) in longissimus dorsi (p>0.05, p<0.001, p<0.001), semimembranosus (p>0.05, p<0.001, p<0.001), triceps brachii (p<0.001, p<0.001, p<0.001), and biceps femoris (p<0.01, p<0.001, p<0.001), respectively. The QQ genotype pigs was associated with increasing meat color traits in the crossbred between Landrace and JNBP. Our findings suggest that the MYH3 6-bp deletion variant genotypes can be used as valuable genetic markers for JNBP-related breeding programs to improve meat quality and control meat color traits.
Keywords
Association; genotypes; Jeju native pig; meat color; MYH3;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Li, B., Dong, C., Li, P., Ren, Z., Wang, H., Yu, F., Ning, C., Liu, K., Wei, W. and Huang, R. 2016. Identification of candidate genes associated with porcine meat color traits by genome-wide transcriptome analysis. Sci. Rep. 6, 35224.   DOI
2 Park, B. Y., Cho, S. H., Kim, J. H., Seong, P. N., Kang, G. H., Jeong, D. W., Kim, C. W., Park, H. C., Jeong, J. H. and Choi, J. S. 2010. Comparison of pork quality by different berkshire line. Food Sci. Anim. Resour. 30, 867-871.   DOI
3 Sambrook, J., Fritsch, E. F. and Maniatis, T., Molecular cloning: a laboratory manual. 1989: Cold spring harbor laboratory press.
4 Seo, S. Y., Park, Y. M., Van-Ba, H., Seo, H. W., Cho, S. H., Park, B. Y., Cho, I. C. and Seong, P. N. 2015. Pork quality characteristics of different parts from crossbred (Jeju Native Pig × Landrace) pigs. J. Agric. Life Sci. 49, 211-223.   DOI
5 Sun, D. W., Seo, H. W., Kim, B. W., Yang, H. S., Joo, S. T. and Lee, J. G. 2013. Breed effects of terminal sires on carcass traits and meat quality in commercial pig industry. Food Sci. Anim. Resour. 33, 125-132.   DOI
6 Van der Wal, P., Engel, B. and Hulsegge, B. 1997. Causes for variation in pork quality. Meat Sci. 46, 319-327.   DOI
7 Jin, S. K., Kim, C. W., Song, Y. M., Jang, W. H., Kim, Y. B., Yeo, J. S., Kim, J. W. and Kang, K. H. 2001. Physicochemical characteristics of longissimus muscle between the Korean native pig and Landrace. Kor. J. Food Sci. Anim. Resour. 21, 142-148.
8 Cho, I. C., Park, H. B., Ahn, J. S., Han, S. H., Lee, J. B., Lim, H. T., Yoo, C. K., Jung, E. J., Kim, D. H. and Sun, W. S. 2019. A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs. PLoS Genet. 15, e1008279.   DOI
9 Cho, S. H., Park, B. Y., Kim, J. H., Kim, M. J., Seong, P. N., Kim, Y. J., Kim, D. H. and Ahn, C. N. 2007. Carcass yields and meat quality by live weight of Korean Native Black Pigs. J. Anim. Sci. Technol. 49, 523-530.   DOI
10 Fortin, A., Robertson, W. and Tong, A. 2005. The eating quality of Canadian pork and its relationship with intramuscular fat. Meat Sci. 69, 297-305.   DOI
11 Kalinowski, S. T., Taper, M. L. and Marshall, T. C. 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol. Ecol. 16, 1099-1106.   DOI
12 Lee, E. Y., Lee, S. J., Hwang, Y. H., Kang, H. J. and Joo, S. T. 2019. Influences of Gender on Meat Quality, Electronic Tongue Measurements and Sensory Characteristics of Pork Loin. Ann. Anim. Resour. Sci. 30, 165-171.   DOI
13 Ryan, T. and Joiner, B. 1976. Normal probability plots and tests for normality: technical report. Statistics Department, The Pennsylvania State University, State College, PA, 1-7.
14 Wang, W., Xue, W., Jin, B., Zhang, X., Ma, F. and Xu, X. 2013. Candidate gene expression affects intramuscular fat content and fatty acid composition in pigs. J. Appl. Genet. 54, 113-118.   DOI
15 Seong, P. N. 2009 A study on value-added products of low fat pork cuts. Ph. D. thesis, Gyeongsang National Univ., Jinju, Korea.