• 제목/요약/키워드: detachment

검색결과 520건 처리시간 0.028초

Effects of Ginsenosides $Rg_3$ and $Rh_2$ OH the Proliferation of Prostate Cancer Cells

  • Kim Hyun-Sook;Lee Eun-Hee;Ko Sung-Ryong;Choi Kang-Ju;Park Jong-Hee;Im Dong-Soon
    • Archives of Pharmacal Research
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    • 제27권4호
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    • pp.429-435
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    • 2004
  • Ginseng has an anti-cancer effect in several cancer models. This study was to characterize active constituents of ginseng and their effects on proliferation of prostate cancer cell lines, LNCaP and PC3. Cell proliferation was measured by $[^3H]$thymidine incorporation, the intracellular calcium concentration by a dual-wavelength spectrophotometer system, effects on mite-gen-activated protein (MAP) kinases by Western blotting, and cell attachment and morphologic changes were observed under a microscope. Among 11 ginsenosides tested, ginsenosides $Rg_3\;and\;Rh_2$ inhibited the proliferation of prostate cancer cells. $EC_{50}s\;of\;Rg_3\;and\;Rh_2$ on PC3 cells were $8.4{\mu}M\;and\;5.5{\mu}M$, respectively, and $14.1{\mu}M\;and\;4.4{\mu}M$ on LNCaP cells, respectively. Both ginsenosides induced cell detachment and modulated three modules of MAP kinases activities differently in LNCaP and PC3 cells. These results suggest that ginsenosides $Rg_3\;and\;Rh_2$-induced cell detachment and inhibition of the proliferation of prostate cancer cells may be associated with modulation of three modules of MAP kinases.

Ornithodoros sawaii (Ixodida: Argasidae) Larvae Collected from Hydrobates monorhis on Sogugul and Gaerin Islands, Jeollanam-do (Province), Republic of Korea

  • Kim, Heung-Chul;Choi, Chang-Yong;Kwon, Young-Soo;Yun, Seok-Min;Lee, Won-Ja;Chong, Sung-Tae;Robbins, Richard G.;Klein, Terry A.
    • Parasites, Hosts and Diseases
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    • 제54권2호
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    • pp.233-238
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    • 2016
  • The 65th Medical Brigade and Public Health Command District-Korea, in collaboration with the Migratory Bird Research Center, National Park Research Institute, conducted migratory bird tick surveillance at Sogugul and Gaerin Islands (small rocky bird nesting sites), Jeollanam-do (Province), Republic of Korea (ROK), on 30 July and 1 August 2009. Breeding seabirds captured by hands in their nesting burrows were banded, identified to species, and carefully examined for ticks during the nesting season. A total of 9 Ornithodoros sawaii larvae were removed from 4 adult Hydrobates monorhis (Swinhoe's storm petrel). The identification of the larvae of O. sawaii collected from migratory seabirds were molecularly confirmed using mitochondrial 16S rDNA primer sets.

Mechanisms of Oblique Shock-Induced Combustion Instability

  • Choi, Jeong-Yeol;Jeung, In-Seuck
    • 한국연소학회지
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    • 제7권1호
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    • pp.23-30
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    • 2002
  • Instability of oblique detonation waves (ODW) at off-attaching condition was investigated through a series of numerical simulations. Two-dimensional wedge of finite length was considered in $H_2/O_2/N_2$ mixtures at superdetonative condition. Numerical simulation was carried out with a compressible fluid dynamics code and a detailed hydrogen-oxygen combustion mechanism. Present result reveals that there is a chemical kinetic limit of the ODW detachment, in addition to the theoretical limit predicted by Rankine-Hugoniot theory with equilibrium chemistry. Result also presents that ODW still attaches at a wedge as an oblique shock-induced flame showing periodically unstable motion, if the Rankine-Hugoniot limit of detachment is satisfied but the chemical kinetic limit is not. Mechanism of the periodic instability is considered as interactions of shock and reaction waves coupled with chemical kinetic effects. From the investigation of characteristic chemical time, condition of the periodic instability is identified as follows; at the detaching condition of the Rankine-Hugoniot theory, (1) flow residence time is smaller than the chemical characteristic time, behind the detached shock wave with heat addition, (2) flow residence time should be greater than the chemical characteristic time, behind an oblique shock wave without heat addition.

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알루미늄 희생층을 이용한 금속 구조물의 제작 (Fabrication of metal structure using AI sacrificial layer)

  • 김정무;박재형;이상호;신동식;김용권;이윤식
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2001년도 하계학술대회 논문집 C
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    • pp.1893-1895
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    • 2001
  • In this paper, novel release technique using wet etch is proposed. The results of this technique and the results of SAMs (Self-Assembled monolayers) coated after release using this technique are compared. Fabricated structure have 100 um in width and experimental length is from 100 um to 1 mm. Thickness of aluminum sacrificial layer is 2 um and structure thickness is 2.5 um. Cantilevers and bridges are fabricated with electroplated gold and silicon nitride deposited on substrate. An aluminium sacrificial layer was evaporated thermally and removed in various wet etching solutions. Detachment length of cantilever is 200 um and detachment length of bridge is 1 mm after isooctane rinsing. And the SAMs coating condition which is appropriate for gold and nitride are studied respectively.

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Effect of Rinsing after Mordanting on the Air-permeability and Dyeing of Fabrics with Cochineal Dyestuff

  • Na, Ho-Jin;Jeon, Dong-Won;Kim, Jong-Jun
    • 패션비즈니스
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    • 제8권6호
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    • pp.137-145
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    • 2004
  • According to the experimental results, it has been reported by several researchers that the air-permeability values of the fabrics mostly decreased by the mordanting during the dyeing procedure. The exact quantitative information, however, has not been presented so far. In this study, the change of the fabric air-permeability was investigated quantitatively. At the same time, the change of the fabric air-permeability according to the dyeing procedure. In order to investigate the possibility of the detachment of the metal ions on the fiber or fabric surface, the change of the air-permeability was investigated after several rinsing of the mordanted fabrics. By comparing the color differences of the cochineal dyed fabrics which were subjected to rinsing procedure after mordanting, the effect of the rinsing of the mordanted fabrics on the dyeing of fabrics was investigated. At the range of mordant concentration, 2% Cu, 2% Sn, 2% Fe, 2% Cr, 5% Al, the metal ions are not excessively absorbed on the fiber surface. Also, any remarkable detachment of the metal ions does not accompany after the mordanting with the subsequent rinsing procedure.

개선된 Dry Air와 SF6의 혼합비에 따른 절연파괴 특성 연구 (A Study on Characteristics of Insulation Breakdown by the Mixing Ratio of enhanced Dry Air and SF6)

  • 석정후;백종현;임동영;배성우;김기채;박원주
    • 조명전기설비학회논문지
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    • 제30권3호
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    • pp.86-93
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    • 2016
  • It is very desirable that a mixture gas possessing excellent insulation performance is suggested for insulation on increasing high voltage. This paper proposes a $SF_6$ mixture gas based on the factors including dielectric strength, environmental impact and economic feasibility of manufacture for the insulation in eco-friendly power equipment. A suitable-$SF_6$ content was determined to improve the dielectric strength in $N_2$ and Dry Air. The examination results of the factors revealed that a $SF_6$/Dry Air mixture gas possessing the $SF_6$ content was more appropriate than a $SF_6/N_2$ mixture gas to the eco-friendly power equipment. In addition to the selection of the suitable $SF_6$ mixture gas, insulation characteristics as a function of $SF_6$ content were described from electron-detachment mechanism.

Colonization of Microbial Biofilms in Pipeline of Water Reuse

  • Kumjaroen, Teratchara;Chiemchaisri, Wilai;Chiemchaisri, Chart
    • Environmental Engineering Research
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    • 제19권3호
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    • pp.275-281
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    • 2014
  • Aim of this study was to investigate biofilms attached in pipeline of water reuse from the MBR system treating sewage without chlorination in correlation to the outflow water quality. Two general pipe materials: polyvinyl chloride (PVC) and polyethylene (PE) were employed in the experiment. The peak growths were found at week 4 in both pipes. The maximum biofilms in PE pipe was $33mgVSS/cm^2$ with the growth rate of $4.75mgVSS/cm^2-d$ which was significant higher than that of PVC pipe. Biofilms examined by PCR-DGGE technique revealed five bacterial species in PE biofilms namely Sinorhizobium medicae WSM419, Sinorhizobium fredii NGR234, Geobacter sp. M18, Parachlamydia acanthamoebae UV-7, and Mycobacterium chubuense NBB4. The VSS concentrations in outflow had directly correlated to the biofilm attachment and detachment. High COD concentrations of outflow appeared during biofilm detaching phase. In summary, water quality of reuse water corresponded to the biofilms attachment and detachment in the pipeline.

Effects of the Chestnut Inner Shell Extract on the Expression of Adhesion Molecules, Fibronectin and Vitronectin, of Skin Fibroblasts in Culture

  • Chi, Yeon-Sook;Heo, Moon-Young;Chung, Ji-Hun;Jo, Byoung-Kee;Kim, Hyun-Pyo
    • Archives of Pharmacal Research
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    • 제25권4호
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    • pp.469-474
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    • 2002
  • The inner shell of the chestnut (Castanea crenata S. et Z., Fagaceae) has been used as an anti-wrinkle/skin firming agent in East Asia, and preliminary experiments have found that a 70% ethanol extract from this plant material can prevent cell detachment of skin fibroblasts from culture plates. In order to examine the molecular mechanisms underlying this phenomenon, its effects on the expression of adhesion molecules, such as fibronectin and vitronectin, were investigated using the mouse skin fibroblast cell line, NIH/3T3. Using fixed-cell ELISA, Western blotting and immunofluorescence cell staining, it was clearly demonstrated that the chestnut inner shell extract enhanced the expression of the cell-associated fibronectin and vitronectin. Scoparone (6,7-dimethoxycoumarin), isolated from the extract, also possessed similar properties. These findings suggest that the enhanced expression of the adhesion molecules may be one of the molecular mechanisms for how the chestnut inner shell extract preventing cell detachment and may be also responsible for its anti-wrinkle/skin firming effect.