• 제목/요약/키워드: derivative work

검색결과 123건 처리시간 0.018초

Streptococcus dysgalactiae로부터 분리된 히알루론산과 황화된 유도체의 구조와 항염증 활성 (Structural Characteristics and Anti-inflammatory Activities of Chemically Sulfated-hyaluronic Acid from Streptococcus dysgalactiae)

  • 홍창일;정의길;한국일;김용현;이성희;이홍섭;한만덕
    • 생명과학회지
    • /
    • 제26권5호
    • /
    • pp.545-554
    • /
    • 2016
  • 히알루론산(HA, Hyaluronic acid)은 β-1, 3-N-acetyl glucosamine과 β-1, 4-glucuronic acid가 반복된 선형 폴리머 고분자로서 생물학적 활성 및 생체친화성 특성 때문에 의약 및 약학분야에서 중요한 분자로 여겨지고 있다. 본 연구는 HA을 S. dysgalactiae으로 얻고, 화학적 방법을 통해 황화된 히알루론산(S-HA, Sulfated hyaluronic acid) 유도체를 합성하여 그 구조와 항염증 활성을 비교하였다. HA의 생산은 S. dysgalactiae를 5 l 생물반응기를 이용하여 대량 배양하여 수용성 히알루론산(HA-WS, water soluble hyaluronic acid)과 비수용성 히알루론산(HA-WI, water insoluble hyaluronic acid)을 분리 정제하였다. 특히 HA-WI를 황화시켜 황화된 히알루론산(S-HA) 유도체를 합성하였으며, 그 수율은 90%로 나타났다. 합성된 S-HA의 구조를 FT-IR 및 1H/13C-NMR를 통해 S. dysgalactiae로부터 생산된 표준 HA, HA-WS 및 HA-WI와 비교 분석한 결과, 황으로 치환된 양상을 확인하였다. 또한, S-HA 의 항염증 활성을 RAW 264.7 대식세포를 통해 확인한 결과, S-HA는 천연 형태의 HA (HA, HA-WS)보다 nitric oxide (NO)와 COX-2 및 PGE2 유전자 발현이 유의하게 낮게 발현되었다. 염증 매개 cytokine인 TNF-α (<80 pg/ml) 및 IL-6 (<100 pg/ml)의 생성도 S-HA가 천연 HA보다 낮은 수준으로 정량되었다. 이 같은 결과에서 황화된 S-HA은 천연 히알루론산보다 용해성이 우수하고 염증관련 사이토카인의 생성 억제를 통해 항염증 효과를 나타내므로 염증치료제, 성형 및 생체 적용 약물전달 소재로 그 활용이 기대된다.

인상주의 회화에서 색채 표현의 기호적 담론 연구 -인상주의 작품 분석을 중심으로 - (A Study on the Semiotic Discourse of Color Expression in Impressionism Paintings -Focus on Works of Impressionism -)

  • 류주현
    • 만화애니메이션 연구
    • /
    • 통권40호
    • /
    • pp.521-549
    • /
    • 2015
  • 우리는 눈으로 보는 행위를 통해 모든 사물을 인지한다. 그러나 눈에 들어온 모든 사물들이 그 사물의 본질일지는 확언할 수 없다. 이에 필자는 여기서 인간이 대상을 보는 것, 그리고 그것을 인지하는 것은 과연 무엇일까라는 의문을 던져본다. 우리가 보는 대상은 '이미지'로서의 대상이며, 따라서 필자는 우리가 대상을 인지한다는 것은 우리의 시감각과 두뇌를 거친 기호작용이라고 보고 있다. 대상들의 본질보다는 우리가 인지하는 데에 '기호'로서 그 대상의 관념이 정의되고 있기 때문이다. 포스트모더니즘 시대에 이르러 예술은 더욱 일종의 정신적 가치를 창출하는 자율적 창조 활동으로서 그것을 지각하는 독자의 삶의 가치를 향유하는데 중대한 역할을 맡고 있다. 인간의 인지에 미적 가치를 부여하는 예술 분야에서 초점을 둔 본 논문은 예술 작품을 시지각적으로 규명하는데 기호작용과 연관지어 분석되었다. 예술이라는 인간의 창작 행위는 상징적이며 기호체계와 밀접하게 연결되어있으며, 나아가 파생된 시각 기호는 그것을 바라보는 주체의 시지각성과 궤도를 나란히 한다고 볼 수 있기 때문이다. 더불어 예술작품을 기호로 인식하여 해석한다면, 색(色)이나 형(形)을 통한 폭넓은 사고로 작품을 음미할 수 있으며, 따라서 필자는 예술 작품과 기호와의 관계성을 분석하는데 색채를 중점으로 둔 색채 지각 논의로 담론화 해보고자 한다. 본 논문은 특히 미술사에서 풍부한 색채를 구사하여 그려오던 인상주의 미술에서의 색채 인식 배경과 색채의 위상 및 표현방법을 바탕으로 색채가 감각 언어로서 독자에게 전달되는 그 상관성을 고찰한다. 작가와 독자 간의 소통으로 이루어지는 예술 작품을 해명하는 데 있어서 색채의 역할은, 예술가의 경험과 투과된 자신의 정서와 생각, 감정 등이 동반되어 작품 안에서 비로소 시각 기호로 사유된다. 이는 색채가 예술작품 안에서 인지 가능한 공간으로 형성될 때 기호의 의미작용을 통해 시각 언어로 해석될 수 있다. 따라서 시각예술을 연구하는데 있어서 본 색채기호 분석이 보다 새로운 텍스트로 거듭나고 향후 효과적인 커뮤니케이션으로 확대되기를 기대해본다.

소규모 기업에 있어서 지식소싱 전략이 기업성과에 미치는 영향 고찰 (An Examination of Knowledge Sourcing Strategies Effects on Corporate Performance in Small Enterprises)

  • 최병구
    • Asia pacific journal of information systems
    • /
    • 제18권4호
    • /
    • pp.57-81
    • /
    • 2008
  • Knowledge is an essential strategic weapon for sustaining competitive advantage and is the key determinant for organizational growth. When knowledge is shared and disseminated throughout the organization, it increases an organization's value by providing the ability to respond to new and unusual situations. The growing importance of knowledge as a critical resource has forced executives to pay attention to their organizational knowledge. Organizations are increasingly undertaking knowledge management initiatives and making significant investments. Knowledge sourcing is considered as the first important step in effective knowledge management. Most firms continue to make an effort to realize the benefits of knowledge management by using various knowledge sources effectively. Appropriate knowledge sourcing strategies enable organizations to create, acquire, and access knowledge in a timely manner by reducing search and transfer costs, which result in better firm performance. In response, the knowledge management literature has devoted substantial attention to the analysis of knowledge sourcing strategies. Many studies have categorized knowledge sourcing strategies into intemal- and external-oriented. Internal-oriented sourcing strategy attempts to increase firm performance by integrating knowledge within the boundary of the firm. On the contrary, external-oriented strategy attempts to bring knowledge in from outside sources via either acquisition or imitation, and then to transfer that knowledge across to the organization. However, the extant literature on knowledge sourcing strategies focuses primarily on large organizations. Although many studies have clearly highlighted major differences between large and small firms and the need to adopt different strategies for different firm sizes, scant attention has been given to analyzing how knowledge sourcing strategies affect firm performance in small firms and what are the differences between small and large firms in the patterns of knowledge sourcing strategies adoption. This study attempts to advance the current literature by examining the impact of knowledge sourcing strategies on small firm performance from a holistic perspective. By drawing on knowledge based theory from organization science and complementarity theory from the economics literature, this paper is motivated by the following questions: (1) what are the adoption patterns of different knowledge sourcing strategies in small firms (i,e., what sourcing strategies should be adopted and which sourcing strategies work well together in small firms)?; and (2) what are the performance implications of these adoption patterns? In order to answer the questions, this study developed three hypotheses. First hypothesis based on knowledge based theory is that internal-oriented knowledge sourcing is positively associated with small firm performance. Second hypothesis developed on the basis of knowledge based theory is that external-oriented knowledge sourcing is positively associated with small firm performance. The third one based on complementarity theory is that pursuing both internal- and external-oriented knowledge sourcing simultaneously is negatively or less positively associated with small firm performance. As a sampling frame, 700 firms were identified from the Annual Corporation Report in Korea. Survey questionnaires were mailed to owners or executives who were most erudite about the firm s knowledge sourcing strategies and performance. A total of 188 companies replied, yielding a response rate of 26.8%. Due to incomplete data, 12 responses were eliminated, leaving 176 responses for the final analysis. Since all independent variables were measured using continuous variables, supermodularity function was used to test the hypotheses based on the cross partial derivative of payoff function. The results indicated no significant impact of internal-oriented sourcing strategies while positive impact of external-oriented sourcing strategy on small firm performance. This intriguing result could be explained on the basis of various resource and capital constraints of small firms. Small firms typically have restricted financial and human resources. They do not have enough assets to always develop knowledge internally. Another possible explanation is competency traps or core rigidities. Building up a knowledge base based on internal knowledge creates core competences, but at the same time, excessive internal focused knowledge exploration leads to behaviors blind to other knowledge. Interestingly, this study found that Internal- and external-oriented knowledge sourcing strategies had a substitutive relationship, which was inconsistent with previous studies that suggested complementary relationship between them. This result might be explained using organizational identification theory. Internal organizational members may perceive external knowledge as a threat, and tend to ignore knowledge from external sources because they prefer to maintain their own knowledge, legitimacy, and homogeneous attitudes. Therefore, integrating knowledge from internal and external sources might not be effective, resulting in failure of improvements of firm performance. Another possible explanation is small firms resource and capital constraints and lack of management expertise and absorptive capacity. Although the integration of different knowledge sources is critical, high levels of knowledge sourcing in many areas are quite expensive and so are often unrealistic for small enterprises. This study provides several implications for research as well as practice. First this study extends the existing knowledge by examining the substitutability (and complementarity) of knowledge sourcing strategies. Most prior studies have tended to investigate the independent effects of these strategies on performance without considering their combined impacts. Furthermore, this study tests complementarity based on the productivity approach that has been considered as a definitive test method for complementarity. Second, this study sheds new light on knowledge management research by identifying the relationship between knowledge sourcing strategies and small firm performance. Most current literature has insisted complementary relationship between knowledge sourcing strategies on the basis of data from large firms. Contrary to the conventional wisdom, this study identifies substitutive relationship between knowledge sourcing strategies using data from small firms. Third, implications for practice highlight that managers of small firms should focus on knowledge sourcing from external-oriented strategies. Moreover, adoption of both sourcing strategies simultaneousiy impedes small firm performance.