• 생물정신의학
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• 제29권1호
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• pp.9-14
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• 2022

Objectives Sleep is fundamental to maintaining homeostatic control and has behavioral and psychological effects on humans. To better understand the function and pathophysiology of sleep, specific gene expressions in reference to sleep deprivation have been studied. In this study, we investigated the gene expression of peripheral blood mononuclear cells after sleep deprivation to better understand the functional consequence of sleep. Methods In eight healthy men, 24 h sleep deprivation was induced. Blood was sampled at 14:00, before and after sleep deprivation. mRNA was isolated and analyzed via microarrays. cDNAs before and after sleep deprivation were coupled to Cy3 or Cy5, respectively, and normalized cDNAs were selected with a ratio greater than two as a significant gene. Results are expressed as mean. Results Among 41174 transcripts, 38852 genes were selected as reliable, and only a small minority (< 1%) of the genes were up-or down-regulated. Total six and eleven genes were selected as significant upregulated and downregulated genes, respectively. Protein tyrosine phosphatase receptor type O was most upregulated (6.9-fold), and low-density lipoprotein receptor-related protein 5-like protein showed the most substantial inhibition (0.06-fold). Conclusions This study showed significant associations between sleep deprivation and the immune system. Acute sleep deprivation affects pathways in proinflammatory cytokines as well as metabolic pathways of glutamate and purine, neurotransmitters related to sleep and wake cycle.

• 동의생리병리학회지
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• 제22권6호
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• pp.1462-1469
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• 2008

The purpose of the study was to confirm what effect HRHDT treatment had on cell extinction by damage of endoplasmic reticulum induced to PC-12 cell damage by glucose deprivation. The study confirmed what effect it had on forming the condition of glucose deprivation within a culture fluid of PC-12 cell and on a nerve cell's survival rates and tested whether HRHDT could prevent extinction of PC-12 cell by glucose deprivation. Also, the study confirmed what effect HRHDT treatment had on the emitted quantity of LDH by glucose deprivation. To examine PC-12 cell's behavioral change under the condition of glucose deprivation and a protective effect of HRHDT on the change, the study observed PC-12 cell's behavioral change with a microscope. Also, the study confirmed density of calcium ion within cells followed by a culture time in the condition of glucose deprivation with FACS and confirmed what effect HRHDT treatment had on the above density of calcium ion within cells. Finally, the study carried out the western blot and confirmed what effect HRHDT treatment had on revelation of GRP 78 and CHOP protein and a segmental type of aspase 12. In this study, HRHDT rescued PC-12 cells from glucose deprivation-induced cell death. HRHDT also prevents the LDH release, Ca++ accumulation, and morphological change, which was associated with the ER stress. Furthermore, HRHDT reduced the expression of ER chaperone (Grp78 and CHOP) proteins by glucose deprivation in PC-12 cells. These results suggest that HRHDT might provide a useful therapeutic strategy in treatment of the neurodegenerative diseases caused by glucose deprivation injuries.

• 한국노년학
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• 제38권2호
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• pp.271-290
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• 2018

이 연구는 사회경제적 박탈 경험이 노인의 자살 생각에 미치는 영향을 분석하는 것을 목적으로 한다. 분석 자료는 한국복지패널 9차년도 자료를 사용하였으며, SPSS를 활용하여 빈도분석, 평균차이분석, 상관분석, 로지스틱 회귀분석 등을 실시하였다. 주요 분석결과는 다음과 같다. 첫째, 공적연금을 받지 못했던 경험, 근로능력이 있으나 실업 상태였던 경험, 경제적 어려움으로 균형 있는 식사를 할 수 없었던 경험, 먹을 것이 떨어졌는데도 더 살 돈이 없었던 경험 등의 순으로 박탈 빈도가 높았다. 둘째, 배우자가 없는 경우, 학력과 소득수준이 낮을수록 자살 생각이 높은 것으로 나타났다. 셋째, 돈이 없어서 본인이나 가족이 병원에 갈수 없었던 경험, 2달 이상 집세가 밀렸거나 집세를 낼 수 없어 집을 옮긴 경험, 먹을 것을 살 돈이 없어 균형 있는 식사를 할 수 없었던 경험, 공과금을 기한 내 납부하지 못한 경험, 근로능력이 있으나 직업을 갖지 못한 경험, 경제적인 어려움 때문에 먹을 것이 떨어졌는데도 더 살 돈이 없었던 경험 등이 노인의 자살 생각에 통계적으로 유의미한 영향을 미치는 것으로 나타났다. 이러한 연구 결과를 토대로 이 논문은 의료급여 사례관리 확대, 노인 주거급여 및 정주 여건 개선, 노인 식생활실태 조사 강화, 노인 공과금 지원 방안 확대 등의 정책 방안을 제시하였다.

• Nutrition Research and Practice
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• 제4권6호
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• pp.455-461
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• 2010

The tumor microenvironment, particularly sufficient nutrition and oxygen supply, is important for tumor cell survival. Nutrition deprivation causes cancer cell death. Since apoptosis is a major mechanism of neuronal loss, we explored neuronal apoptosis in various microenvironment conditions employing neuroblastoma (NB) cells. To investigate the effects of tumor malignancy and differentiation on apoptosis, the cells were exposed to poor microenvironments characterized as serum-free, low-glucose, and hypoxia. Incubation of the cells in serum-free and low-glucose environments significantly increased apoptosis in less malignant and more differentiated N-type IMR32 cells, whereas more malignant and less differentiated I-type BE(2)C cells were not affected by those treatments. In contrast, hypoxia (1 % $O_2$) did not affect apoptosis despite cell malignancy. It is suggested that DLK1 constitutes an important stem cell pathway for regulating self-renewal, clonogenicity, and tumorigenicity. This raises questions about the role of DLK1 in the cellular resistance of cancer cells under poor microenvironments, which cancer cells normally encounter. In the present study, DLK1 overexpression resulted in marked protection from apoptosis induced by nutrient deprivation. This in vitro model demonstrated that increasing severity of nutrition deprivation and knock-down of DLK1 caused greater apoptotic death, which could be a useful strategy for targeted therapies in fighting NB as well as for evaluating how nutrient deprived cells respond to therapeutic manipulation.

• 한국지역사회생활과학회지
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• 제17권4호
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• pp.81-95
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• 2006

This study was made to find out how stress affect on nutrition status of the college freshmen who were experiencing physical growth and development as well as drastic emotional change. 400 male and female freshmen in 4 year colleges were surveyed respectively through the health check-up procedure for college entrance in February, In order to find out the stress in each group frustration, deprivation, lack of self efficacy, type A behavior and anxiety response were surveyed through 10 questions with total 40 points by assigning 4 points for each question. Diet Status was expressed by DDS (Dietary Diversity Score by 5 food groups) and DVS(Dietary Variety Score). 24-hrs recall method was used to find out the quantity of daily nutrient of EAR(estimated adquacy ratio) by KDRIs(Korean Dietary Recommended Intakes). Nutrition level was analyzed by Can-Pro for professionals (Korea Nutrition Association). And for the quality intake, percentage was calculated and MAR(Mean Adequacy Ratio) were produced. Highest point was obtained in the stress of anxiety with the total 40 score of 30.20, and the scores were 29.79, 28.67, and 28.39 for deprivation, type A behavior and frustration respectively. There was no difference of blood components in accordance with stress type. Stress type was divided into less sensitive group and highly sensitive one and the relationship with the blood nutrient status was observed. The difference of blood component and blood pressure in sensitive and highly sensitive groups was observed in deprivation and anxiety. The index of blood pressure(p<0.05), hemoglobin(p<0.01), HDL-cholesterol(p<0.05), and Fe(p<0.05) was high in the deprivation of sensitive group. Blood pressure and hemoglobin was high in type A of sensitive group(p<0.05). And the contents of blood triglyceride was high in the anxiety of sensitive group(p<0.001) The result of nutrition intake analysis according to stress type showed that there was low intake for energy, riboflavin, and niacin. When the degree of deprivation was high there was a lack of riboflavin intake and there was no significant difference of nutrition intake in lack of self efficacy, type A behavior and anxiety response. Thus, it is necessary for colleges to educate the students to maintain mental stability through various programs and activities after catching a kind and extent of the stress college students we meeting with like the confusion of value system, open heterosexual relationship, and the employment difficulties linked with political uncertainty and economic recession.

• 보건교육건강증진학회지
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• 제26권4호
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• pp.11-22
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• 2009

Objectives: The researchers investigated the relationship between sleep pattern (circadian rhythm/sleep deprivation), eating habit, and the perceived skin condition of female adults, in orders to provide valuable information to women who want to maintain healthy skin and professionals in health promotion. Methods: The participants were 297 female adults whose ages ranged from 20 to 60 (M=35.14, SD=10.37). The questionnaires and psychological tests used in this research included the following: Circadian Rhythm Questionnaire, Sleep Deprivation Scale, Eating Habit Questionnaire, Skin Condition Questionnaire, Scale for Perceived Skin Health. Results: Results indicated that evening type women had more fatty skin and felt their skins less healthier than morning type. Although women who did not deprived their sleep had more fatty and sensitive skin and felt their skins less healthier than women who deprived their sleep, sleep deprivation was positively related to the morningness, and the morningness was negatively related to the preference of fatty and spicy foods. Only the preference of fatty foods among eating habit was positively related to the fatty and sensitive skin, and negatively to the perceived skin health. Regression analyses with circadian rhythm and the preference of fatty foods revealed that only circadian rhythm was significant predictor for the fatty skin, while the preference of fatty foods was only significant predictor for the sensitive skin and the perceived skin health. And, circadian rhythm and the preference of fatty foods accounted for around 12.0% variance of the fatty skin. Conclusion: This study reiterates the roles of fatty foods on skin health, and found the role of circadian rhythm on skin health, and it is needed to explore the relationship between sleep deprivation and skin condition further. These results may provide useful information for health practitioners.

• 보건행정학회지
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• 제31권1호
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• pp.114-124
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• 2021

Background: In this study, we aimed to investigate the interaction effects of individual socioeconomic status and regional deprivation on the onset of diabetes complications and diabetes-related hospitalization among type 2 diabetes patients. Methods: Korean National Health Insurance Service National Sample Cohort data from 2002 to 2013 were used. A total of 50,954 patients newly diagnosed with type 2 diabetes from 2004 to 2012 and aged 30 years or above were included. We classified patients into six groups according to individual income level and neighborhood deprivation: 'high in advantaged,' 'high in disadvantaged,' 'middle in advantaged,' 'middle in disadvantaged,' 'low in advantaged,' and 'low in disadvantaged.' We calculated hazard ratios (HR) of onset of diabetes complication and diabetes-related hospitalization using the Cox proportional hazard model, with the reference group as diabetes patients with high income in advantaged regions. Results: In terms of the interaction effects of individual income level and regional socioeconomic level, even with the same low individual income level, the group with a high regional socioeconomic level (low in advantaged) showed low HRs for the onset of diabetes complication (HR, 1.04; 95% confidence interval [CI], 1.00-1.08) compared to the 'low in disadvantaged' group (HR, 1.10; 95% CI, 1.05-1.16). In addition, the 'high in advantaged' group showed slightly higher HRs for the onset of diabetes complication (HR, 1.06; 95% CI, 1.00-1.11) compared to the 'low in advantaged' and it appeared to be associated with slight mitigation of the risk of diabetes complication. For the low-income level, the patients in disadvantaged regions showed the highest HRs for diabetes-related hospitalization (HR, 1.29; 95% CI, 1.19-1.41) compared to the other groups. Conclusion: Although we need to perform further investigations to reveal the mechanisms that led to our results, interaction effects individual socioeconomic status and regional deprivation might be associated with on onset of diabetes complications and diabetes-related hospitalization among type 2 diabetes patients.

• 한국식품영양과학회지
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• 제42권8호
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• pp.1167-1174
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• 2013

암세포를 포함한 생체 내 빠른 분열을 요구하는 세포 집단에서 세포 내 구성요소 및 에너지원으로서 글루타민의 요구량이 증대되지만, 종양세포의 글루타민 의존적 대사작용에 관한 기전은 여전히 잘 알려진 바 없다. 본 연구에서는 LnCaP 전립선 암세포의 이동성 및 침윤성에 미치는 글루타민 결핍효능을 조사하였다. 본 연구의 결과에 의하면 LnCaP 세포에서 글루타민 결핍에 의하여 세포의 이동성 및 세포의 침윤성이 현저하게 억제되었으며, 이러한 이동성 및 침윤성 억제는 TIMPs의 발현 증대에 의한 MMPs의 발현 감소 및 그들의 효소적 활성 저하와 연관성이 있었다. 또한 글루타민이 결핍된 조건에서 배양된 LnCaP 세포에서 TER의 현저한 증가가 관찰되었는데, 이는 TJs의 조절인자인 claudin family 발현의 차단에 의한 것으로 생각되어진다. 본 연구의 결과에 의하면 암세포의 증식에서 글루타민의 결핍은 TJ의 결합력 증대와 MMPs의 활성을 저하시킴으로써 암세포 전이에 가장 기본적인 과정인 암세포의 이동성과 침윤성을 억제시킬 수 있을 것으로 생각된다.

• Animal cells and systems
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• 제12권4호
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• pp.211-217
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• 2008

Bax, a pro-apoptotic member of Bcl-2 family proteins, plays a central role in the mitochondria-dependent apoptosis. Apoptotic signals induce the translocation of Bax from cytosol into the mitochondria, which triggers the release of apoptogenic molecules such as cytochrome C and apoptosis-inducing factor, AIF. Bax-inhibiting peptide(BIP) is a cell permeable peptide comprised of five amino acids designed from the Bax-interaction domain of Ku70. Because BIP inhibits Bax translocation and Bax-mediated release of cytochrome C, BIP suppresses Bax-dependent apoptosis. In this study, we observed that BIP inhibited staurosporine-induced neuronal death in cultured cerebral cortex and cerebellar granule cells, but BIP failed to rescue granule cells from trophic signal deprivation-induced neuronal death, although both staurosporine-induced and trophic signal deprivation-induced neuronal death are dependent on Bax. These findings suggest that the mechanisms of the Bax activation may differ depending on the type of cell death induction, and thus BIP exhibits selective suppression of a subtype of Bax-dependent neuronal death.

• 한국가금학회지
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• 제17권1호
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• pp.33-44
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• 1990

본 연구는 육계와 육용오리의 도체특성을 비교하고 육계의 성, 사육형질, 계절 및 절식시간에 따른 도체율과 복강지방축적률을 조사하기 위하여 육계 240수와 육용오리 20수에 대한 도체조사기록을 이용하여 연구하였는바 그 결과를 요약하면 다음과 같다. 1. 육계와 육용오리의 도체률은 각각 65.43%와 66.79%였으며, 복강지방축적률은 육계가 육용오리가 비하여 현저하게 높았다. 2. 육계와 육용오리의 부분육 생산비율은 현저한 차이가 있었으며, 육계의 경우 부분육 생산비율은 다리, 가슴, 등, 날개 및 목의 순서로 높았다. 3. 육계의 도체율은 성별에 따라 차이가 없었으나 복강지방축적율은 암컷이 수컷에 비하여 현저하게 높았다. 4. 사육형태간에 도체율은 차이가 없었으나 복강지방축적율은 케이지가 평사에 비하여 다소 높은 경향이었다. 5. 사육계절간에도 도체율은 차이가 없었으나 복강지방축적률은 하계에 가장 높았고 동계에 가장 낮았다. 6. 절식ㆍ절수시간이 1시간 경과함에 따라 육계의 체중은 약0.28365%씩 감소하였으며, 도체율은 절식 6시간 후 가장 높았다.